Heparin-binding proteins from boar seminal plasma affecting the release of prostaglandins and interleukin-6 by porcine endometrial and cervical cells and bovine endometrial cells

The objectives of this study were to explore whether heparin-binding proteins, separated by fast protein liquid chromatography from boar seminal plasma influence the release of prostaglandins F2α, (PGF2α), E2 (PGE2) and interleukin-6 (IL-6) by porcine endometrial and cervical cells and even bovine endometrial cells. In Experiment I, we showed that release of PGF2α by endometrial epithelial, endometrial stromal and cervical stromal cells to the medium was inhibited (p α (TNFα) stimulated release of IL-6 by endometrial and cervical stromal cells after 24 h incubation, but in the presence of heparin-binding proteins, this stimulation was attenuated. Release of PGF2α by cryopreserved (Experiment II) and primary (Experiment III) cervical stromal cells was significantly inhibited after 3 h incubation with 66 - 95.4 μg of heparin- binding proteins. A significant inhibition of PGE2 release by cryopreserved and primary cervical stromal cells was already achieved after incubation with 16.5 - 23.9 μg of heparin-binding proteins. The release of IL-6 by cryopreserved cells was stimulated after 3 h incubation with heparin- binding proteins in a dose dependent manner in contrast to the release of IL-6 by freshly isolated cervical stromal cells. We also found (Experiment IV) that porcine heparin-binding seminal plasma proteins inhibited release of PGF2α and stimulated release of IL-6 by bovine endometrial epithelial cells. In conclusion, a group of heparin-binding proteins separated by fast protein liquid chromatography from boar seminal plasma inhibit PGF2α, PGE2 and stimulate IL-6 release by porcine endometrial and cervical cells and even by bovine endometrial cells. Thus, these proteins have a similar effect as the entire seminal plasma.

心脏移植后急性排异反应体外模型的建立及其应用研究

分离成年Ｗｉｓｔａｒ大鼠心肌细胞（２×１０６ｍｌ－１）ＳＤ大鼠的脾细胞（１×１０７ｍｌ－１），前者做刺激细胞，后者做反应细胞，进行混合细胞培养，建立大鼠心脏移植后急性排异反应体外模型。该模型包括４组：①阴性对照组，心肌细胞与经丝裂霉素Ｃ处理的脾细胞共同培养。②阳性对照组，心肌细胞与脾细胞共同培养。③药物对照组，在阳性组基础上加入ＣＳＡ（环孢霉素Ａ１０μｇ／ｍｌ）。④实验组，在阳性组基础上加入实验药物（ＨＳＰ，人类精浆，１０μｇ／ｍｌ）。应用光镜、电镜观察各组心肌细胞的受损情况和脾细胞转化为淋巴母细胞的数量，并用３Ｈ－ＴｄＲ掺入法测定脾细胞的转化率，证实了ＣＳＡ和ＨＳＰ有相似的免疫抑制作用。用大鼠腹腔内心脏异位移植体外模型来重复验证ＣＳＡ和ＨＳＰ的免疫抑制作用，两者结果相同，从而证明了心肌细胞混合培养的体外模型不仅简便易行，可靠性强，而且为大规模地筛选免疫抑制剂提供了一种方便、廉价的实验工具。

美国主流媒体表达中国文化特色词汇的显异策略——基于对2009年《纽约时报》涉华报道的实证研究

本文以2009年全年《纽约时报》涉华报道中所有译入英语的中国文化特色词汇为研究样本,通过定量分析证实了《纽约时报》在表达中国文化特色概念时以异化为主的翻译策略,并分析了该报在处理中国文化特色词汇时的种种"显异"手法;本文讨论了以上研究结果对我国外宣翻译的种种启示,并认为在保证国外读者理解的前提下,应优先采用异化方法翻译中国文化特色词汇,以达到传播中华文化,展示中国文化软实力的目的。

精液液化异常者精浆四种生化指标检测及其临床意义

目的：探讨精液液化异常时精浆生化指标的变化。 方法：选择１１８ 例精浆样本，其中正常生育组３６ 例，液化正常组 ５４ 例，液化异常组２８ 例，分别测定了精浆柠檬酸、酸性磷酸酶、唾液酸、果糖的含量。 结果：精液液化异常时，精浆柠檬酸和酸性磷酸酶显著下降，而精浆唾液酸、果糖无明显变化。 结论：精液生化成分的改变，是导致精液不液化的重要原因之一。

浙南华侨华人与中欧文化交流

不同文化之间需要双向互动交流。中外文化交流内容包括人员往来、物产移植、衣食住行、婚丧嫁娶等风俗习惯的互相影响,以及宗教、文学、艺术方面的传播等等。在中欧文化交流中,浙南华侨华人充当了重要媒介。他们将中国传统文化重要组成部分的浙南地域文化传到欧洲,使之成为欧洲多元文化的组成部分之一。随着他们与家乡联系和沟通的加强,又把部分欧洲文化传回故土,对侨乡文化的发展产生重要影响。

陈寅恪与兰克史学

陈寅恪在日本、美国和德国留学期间接受了兰克史学的熏陶。陈寅恪的史学与兰克史学多有相似之处:史学方法上强调广泛占有资料并严加考证;在史学思想、史学观点方面奉行人文主义、客观主义,注意运用民族(种族)文化观念治史。陈氏归国后又在大学教学中实行了“习明纳尔”这种教学方法。