Water-soluble crude polyseccharide（PIP） was extracted from cultured mycelium of the fungus Phellinus igniarius. After ethanol precipitation and sepharose CL-6B gel filtration, the fraction of PIP1 was obtained, whic...Water-soluble crude polyseccharide（PIP） was extracted from cultured mycelium of the fungus Phellinus igniarius. After ethanol precipitation and sepharose CL-6B gel filtration, the fraction of PIP1 was obtained, which was shown to be a homogeneous polysaccharide by means of high-performance liquid chromatography. The structure of PIPt was determined by using several methods. C.,C analysis indicates that PIP1 is composed of the monosaccharides of glucose, galactose, and mannose. Their malar ratio is 3. 70： 4. 06： 1.00. The molar weight was estimated to be 17 kd via HPLC. IR, GC, partial hydrolysis with acid, pefiedate oxidation, Smith degradation, methylation, and GC-MS analysis were used for the structural analyses of PIP1. The results show that PIP1 has a small quantity of branch structure, The main glycosidic linkage of PIP1 has a β-configurafion. The main chain is made up of a large mass of glucose （ 1→3 ） and few mannose （ 1→4 ） ; the side chain is composed of glucose （ 1 →3 ） and galactose （ 1→6 ） ; the nonreduced end is composed of galactose and glucose. The side chains are branched at 6-0 of glucose（ 1→3,6） and mannose（1→4,6）. On an average, there are three branches among 20 residues. It is presumable that the existence of 1,3-linked Glc in the main and side chains is the main reason for its higher antitumor activity.展开更多
In order to expand the sources of raw materials for edible fungus cultivation, reduce the use of wood, and realize the harmless treatment and efficient utilization of bamboo sawdust, bamboo sawdust was used to partial...In order to expand the sources of raw materials for edible fungus cultivation, reduce the use of wood, and realize the harmless treatment and efficient utilization of bamboo sawdust, bamboo sawdust was used to partially replace the broad-leaved wood sawdust in the conventional formula, and the growth of Ganoderma lucidum and Stropharia rugosoannulata mycelia in a large test tube in different matrix formulas was studied. The results show that the mycelia of the two edible fungi could grow normally in the matrices with bamboo sawdust;the growth of the mycelia in various formulas was different, and the performances of different strains of the same species were also different. Compared with the conventional formula, the suitable substitution amount of bamboo sawdust for the G. lucidum strains was 30%-45%, and that for S. rugosoannulata strains was 16%-32%.展开更多
基金Supported by the Science and Technique Bureau of Wenzhou City(No. S2005A003).
文摘Water-soluble crude polyseccharide（PIP） was extracted from cultured mycelium of the fungus Phellinus igniarius. After ethanol precipitation and sepharose CL-6B gel filtration, the fraction of PIP1 was obtained, which was shown to be a homogeneous polysaccharide by means of high-performance liquid chromatography. The structure of PIPt was determined by using several methods. C.,C analysis indicates that PIP1 is composed of the monosaccharides of glucose, galactose, and mannose. Their malar ratio is 3. 70： 4. 06： 1.00. The molar weight was estimated to be 17 kd via HPLC. IR, GC, partial hydrolysis with acid, pefiedate oxidation, Smith degradation, methylation, and GC-MS analysis were used for the structural analyses of PIP1. The results show that PIP1 has a small quantity of branch structure, The main glycosidic linkage of PIP1 has a β-configurafion. The main chain is made up of a large mass of glucose （ 1→3 ） and few mannose （ 1→4 ） ; the side chain is composed of glucose （ 1 →3 ） and galactose （ 1→6 ） ; the nonreduced end is composed of galactose and glucose. The side chains are branched at 6-0 of glucose（ 1→3,6） and mannose（1→4,6）. On an average, there are three branches among 20 residues. It is presumable that the existence of 1,3-linked Glc in the main and side chains is the main reason for its higher antitumor activity.
基金Supported by the Science and Technology Project of Guizhou Province ((2022)YB176,[2020]1Y073,(2019)2773,[2019]3007)。
文摘In order to expand the sources of raw materials for edible fungus cultivation, reduce the use of wood, and realize the harmless treatment and efficient utilization of bamboo sawdust, bamboo sawdust was used to partially replace the broad-leaved wood sawdust in the conventional formula, and the growth of Ganoderma lucidum and Stropharia rugosoannulata mycelia in a large test tube in different matrix formulas was studied. The results show that the mycelia of the two edible fungi could grow normally in the matrices with bamboo sawdust;the growth of the mycelia in various formulas was different, and the performances of different strains of the same species were also different. Compared with the conventional formula, the suitable substitution amount of bamboo sawdust for the G. lucidum strains was 30%-45%, and that for S. rugosoannulata strains was 16%-32%.
