Repetitive administration of cultured human CD34+cells improve adenine-induced kidney injury in mice

BACKGROUND There is no established treatment to impede the progression or restore kidney function in human chronic kidney disease(CKD).AIM To examine the efficacy of cultured human CD34+cells with enhanced proliferating potential in kidney injury in mice.METHODS Human umbilical cord blood(UCB)-derived CD34+cells were incubated for one week in vasculogenic conditioning medium.Vasculogenic culture significantly increased the number of CD34+cells and their ability to form endothelial progenitor cell colony-forming units.Adenineinduced tubulointerstitial injury of the kidney was induced in immunodeficient non-obese diabetic/severe combined immunodeficiency mice,and cultured human UCB-CD34+cells were administered at a dose of 1×106/mouse on days 7,14,and 21 after the start of adenine diet.RESULTS Repetitive administration of cultured UCB-CD34+cells significantly improved the time-course of kidney dysfunction in the cell therapy group compared with that in the control group.Both interstitial fibrosis and tubular damage were significantly reduced in the cell therapy group compared with those in the control group(P<0.01).Microvasculature integrity was significantly preserved(P<0.01)and macrophage infiltration into kidney tissue was dramatically decreased in the cell therapy group compared with those in the control group(P<0.001).CONCLUSION Early intervention using human cultured CD34+cells significantly improved the progression of tubulointerstitial kidney injury.Repetitive administration of cultured human UCB-CD34+cells significantly improved tubulointerstitial damage in adenine-induced kidney injury in mice via vasculoprotective and anti-inflammatory effects.

Impacts of Corporate Culture on Merger

By studying the cultural background and corporate culture in China, Japan and USA, this paper analyzes the effects of cultural background and corporation culture on the transaction cost and the efficiency of control right, thus shows that cultural backgrounds have great impact on merger. This paper further studies the choice and application of the mode of culture combination of the transnational merger.

Relationship between Tomato (Solanum lycopersicum) Nutrition and the Severity of the Vascular Fusariosis

The vascular fusariosis is one of the main obstacles in the tomato crop production. Currently, the management of the nutrient solution is presented as a control option for fusariosis, for such reason different nutrient solutions with nitrogen (N), phosphorus (P), potassium (K) and calcium (Ca) modifications;in four saladette tomato varieties Bony Best (BB), Manapal (M), Walter (W) and FLA were evaluated in this research, in order to analyze the damage caused by Fusarium oxysporum f.sp. lycopersici (FOL) severity. Tomato plants were inoculated with 3 FOL breeds, establishing a completely random design with a factorial arrangement with six repetitions. Disease’s severity was evaluated 30 days after the inoculation using levels of (0 - 4) of the methodology established by Marlatt and Correll (1988). Afterwards, a statistical analysis was done through the Kruskal Wallis test, in which it was observed that treatments 93 (Ca 207 mg∙L-1, R3, variety BB), 95 (207 mg∙L-1 Ca, R3, variety W), 81 (0Ca, R3, BB) showed less disease severity. In the foliar analysis, the best treatments were 22 (193 mg∙L-1 N, R3, M) for N, the greatest P content was treatment 94 (207 mg∙L-1 Ca, R3, M) for K treatment three and 93 (207 mg∙L-1 Ca, R3, variety BB) greatest Ca content.

The ecological effects of rice straw mulching on vineyard

Yongzhou often encounters drought condition in July and August. Sometimes no rainfall lasts for 20 to 40 days, causes a great damage to grape production Our four-year rice straw mulching test on vineyard indicates that if favorable mulching is supplied properly, it reduces soil temperature and conserves soil moisture in summer, increases soil temperature in winter, keeps soil loose and mellow, depresses weeds, and increases soil organic matter content in vineyard to secure good quality and higher yield even under drought condition.