An efficient culture procedure for micro-propagation of rice regeneration plants

In this paper,we describe briefly an efficientculture procedure for micro-propagation of riceregeneration plants.The procedure consists ofthe following steps: 1.Preparation of materials Calli wereinduced from mature seeds of an indica rice lineG67 by culturing the husked and disinfectedseeds on agar medium,consisting of Nbasalelements,3％ sucrose,1000mg/L proline,and 32mg/L 2,4-D.After one month＇s in- duction culture and 3 wk subculture,compactand nodular calli were picked out and trans- ferred into liquid medium for suspension cul-tures.The liquid medium contained the same

Effect of agar concentration in media on green plantlet regeneration frequency in rice anther culture

We studied the effect of agar concentration inmedia on callus induction rate and green plant-let regeneration frequency in rice.Materialswere Fgeneration of indica/indica or indica/japonica,which were 96E76(Hei’e/Zhaiye- qing 8),96E80[(IR 24/Guanglu＇ai 4//Zhongnan’ai)/Yifengzao],96E86(Zhong- munong 9/Zhaiyeqing 8).The induction mediaused was M8+2mg/L 2,4-D,and agar con-centrations were 0.6％,0.8％,and 1.0％,respectively.Regeneration media was MS+2mg/L KT+0.5mg/L IAA+0.5mg/LNAA,and agar concentrations were 0.6％ and1.0％.Results indicated that the calli induc-

NEUROBIOLOGICAL EFFECTS OF NRCF DERIVED FROM DISTAL STUMPS OF MOTOR NERVE AND SENSORY NERVE AND b-FGF ON CULTURED MOTONEURON IN VITRO

Objective: To explore the mechanism of neurotropism in peripheral nerve regeneration by assessing the bioactivity of regeneration microcircumstance on motoneurons. Methods The motor branch Of femoral nerve to quadriceps was incised and the distal stump was sutured with one-end silicone chamber. The nerve regeneration chamber fluids from distal motor nerve stumps (motor branch of femoral nerve ) (MD-NRCF) was collected 7d post-operatively, and with the same method, nerve regeneration conditioned fluids from distal stumps nerve stumps (saphenous nerve ) (SD-NRCF) was collected. The dissociated rat’s motoneurons were co-cultured with MD-NRCF, SD-NRCF, basic fibroblast growth factor (b-FGF) and serum-free medium for 72h respectively and then were photographed under phase-contrast microscope. The longest neurites and cellbody areas of motoneurons from each group were measured by cell image processing computer system. MTT colorimetric assay was also used to measure cell activation. Results The cells of MD-NRCF group had significantly longer neurites than the other 3 groups, and their activation was also superior to those of the other groups. Conclusion These results indicate that MD-NRCF has more significant neurite-promoting and neurobiological effects on motoneuron than SD-NRCF and b-FGF.

Examining the properties and therapeutic potential of glial restricted precursors in spinal cord injury

In the aftermath of spinal cord injury,glial restricted precursors（GRPs） and immature astrocytes offer the potential to modulate the inflammatory environment of the injured spinal cord and promote host axon regeneration.Nevertheless clinical application of cellular therapy for the repair of spinal cord injury requires strict quality-assured protocols for large-scale production and preservation that necessitates long-term in vitro expansion.Importantly,such processes have the potential to alter the phenotypic and functional properties and thus therapeutic potential of these cells.Furthermore,clinical use of cellular therapies may be limited by the inflammatory microenvironment of the injured spinal cord,altering the phenotypic and functional properties of grafted cells.This report simulates the process of large-scale GRP production and demonstrates the permissive properties of GRP following long-term in vitro culture.Furthermore,we defined the phenotypic and functional properties of GRP in the presence of inflammatory factors,and call attention to the importance of the microenvironment of grafted cells,underscoring the importance of modulating the environment of the injured spinal cord.

Rural Elite Flow and Protection of Intangible Cultural Heritage in the Social Transformation Period

Under the violent impact of the social transformation period,traditional cultural heritage faces a great crisis of protection,inheritance and development.In the context of collapse of basic structure of traditional rural society,the protection of traditional cultural heritage becomes an important task in the new period.Although both the state and the society attach great importance to the protection of intangible cultural heritage,it still faces a huge crisis of disappearance.In the system,this is because cultural elite list system and intangible cultural heritage protection system are confronted with many practical problems.The cultural elite of the rural society,as main group of intangible culture inheritance,is always ignored and this has become a hidden trouble for cultural heritage protection.In this situation,it is recommended to constantly restore excellent cultural ecological environment,improve the supporting facilities of the intangible cultural protection system,cultivate cultural elites of rural areas at the level of endogenous driving force,improve the rural centripetal force centering on cultural elites,restore its functions in transmitting culture and guiding value in rural society,and rebuild the folk cultural elite regeneration mechanism,so as to real y push the intangible cultural heritage protection towards the sustainable development of inheritance.

Plant regeneration from dry mature endosperm cultures of Eucommia ulmoides Oliv.

EUCOMMIA ulmoides is not only a famous medicinal plant,but also a most promising rubber-source plant in the temperate zone.All the individuals present in this species are diploid(2n=2x=34),and the objective of this work is to establish a novel triploid type of it so as to im-prove its overall biomass output or rubber content in the leaf cells by the characteristic cell en-