Investigation of the Acceptability of Cultured Meat in University Students

Background: Over the past 20 years, cultured meat has drawn a lot of public attention as a potential solution to issues with animal husbandry, including inadequate use of natural sources, improper animal welfare practices, and possible risks to public health and safety. The novel method of producing meat through culture reduces the need for animals to produce muscle fiber, thereby obviating the necessity for animal slaughter. Apart from its ethical advantages, cultured meat presents a possible way to fulfill the expanding need for food among growing populations. The purpose of this research was to find out whether Turkish students would be willing to pay for and accept cultured meat. Technique: Method: 371 university students who willingly consented to fill out a questionnaire and provide demographic data make up the research sample. Questions from previous studies on the acceptability of cultured meat were compiled to create the survey. The research’s data collection took place in March and April of 2022. The research was completed in June 2022 after the data had been processed and analyzed. Results: The results showed that the majority of participants were female and had omnivorous eating habits. Based on the results of the Bonferroni correction test, students with a higher intention to purchase and consume cultured meat were those who received economics and business education. Students with two years of university education had a higher overall survey score than those with four years of education (p < 0.05). Furthermore, it is discovered that there is a negative correlation between the participants’ ages and their Factor 2 (using cultured meat as an alternative to industrial meat) and Factor 3 (consuming and purchasing it) section points (r = -109, p = 0.036) (r = -0.121, p = 0.019). In conclusion, university students generally have a negative outlook on health-related issues, such as eating cultured meat as an alternative.

A comparative study on the contents of vitamins in the natural and the cultured prawn

A systematically comparative study on the contents of vitamins in the natural and the cultured prawn, Penaeus chinensis, was made by using High-efficiency Liquid Phase Chromatography in 1989. The results show that the vitamin C (Vc) content in the natural prawn was 8. 19% higher than that of the cultured prawn.As the natural prawn's body length increasing, the Vc content in cephalothorax increased continuously, but after August 10, the Vc content in the cultured prawn fell down rapidly. No significant differences in vitamin B1, B2,B6, nicotinic acid (NAc), nicotinamide (NAm) and folio acid (FAc) were found between the natural and the cultured prawn. Therefore, Vc probably is one of major factors limiting the growth and development of Chinese prawn.

Establishment and evaluation of the primary cultured tibial osteoblast model of broiler chicks

Osteoblasts are considered as a major factor contributing to bone development and mineralization,however,few studies have been done to establish and evaluate the primary cultured tibial osteoblast model of broiler chicks.Therefore,in the present study,two experiments were conducted to establish and evaluate the primary cultured tibial osteoblast model of broiler chicks.In experiment 1,osteoblasts were isolated from the tibia of one-day-old Arbor Acre male broiler chicks using the explant method and identified through the cell morphology,alkaline phosphatase(ALP)and alizarin red staining.Experiment 2 was carried out to evaluate the vitality and mineralization of primary cultured tibial osteoblasts of broilers on days 4,8,12,16,20,24,28 and 32 after incubation,respectively.The results from experiment 1 demonstrated that primary cultured tibial osteoblasts of broilers showed a spindle-shaped,triangular or polygonal morphology.More than 95%of the cells were stained blue-black after ALP staining,and mineralized nodules were formed after 4 days of continuous incubation.In experiment 2,lactate dehydrogenase(LDH)activity stayed at a relatively stabilized level although incubation time affected(P=0.0012)it during the whole culture period.Additionally,incubation time affected(P≤0.0001)the number and proportion of the area of mineralized nodules.They increased linearly and quadratically(P<0.04)with the increase of incubation time,and remained at a stabilized level from 24 to 32 days of incubation.The estimates of the optimal incubation time were 17 and 26 days based on the best fitted broken-line or quadratic models(P<0.0001)of the number and proportion of the area of mineralized nodules,respectively.These results indicate that the primary cultured tibial osteoblast model of broilers has been established successfully by the explant method,and it showed typical osteoblast morphology and characteristics of ALP activity and mineralization,and could maintain a relatively stabilized vitality from 4 to 32 days of incubation;and the optimal incubation time of primary tibial osteoblasts was 17 to 26 days.Therefore,it could be used to further study the underlying mechanisms of bone development and mineralization of broiler chicks.

Analysis and Evaluation of Nutrient Composition in the Wild, Pond- and Lake-Cultured Topmouth Culter (Culter alburnus)

Research was conducted on topmouth culter (Culter alburnus) grown in ponds and lakes as well as wild types in order to determine their chemical composition and nutritional value. There are three types of fish that differ in their proximate composition, amino acids, fatty acids, and minerals. Wild fish had a significantly lower crude lipid contents than cultured fish (P P ∑PUFAs) showed an obviously opposite trend. As compared with cultured fish, wild fish had significantly higher levels of n-3 PUFAs, arachidonic acid (AA), eicosapentaenoic acid (EPA) and docosahxaenoic acid (DHA) (P P > 0.05), except for Na, Fe and Se. In conclusion, diet composition and external aqueous environment may determine the differences between wild and cultured topmouth culter.

Sequential extraction of RNA,DNA and protein from cultured cells of the same group

BACKGROUND Efficient extraction of nucleic acids and proteins(ENAP)from cells is a prerequisite for precise annotation of gene function,and has become laboratory routine for revealing the mysteries of life.However,cell samples are often from different culture dishes,resulting in inevitable experimental errors and sometimes poor repeatability.AIM To explore a method to improve the efficiency of ENAP,minimizing errors in ENAP processes,enhancing the reliability and repeatability of subsequent experimental results.METHODS A protocol for the sequential isolation of RNA,DNA,and proteins from the same cultured HepG2 cells using RNAzol reagent is presented here.The first step involves culturing HepG2 cells to the exponential phase,followed by the sequential isolation of RNA,DNA,and proteins from the same cultured cells in the second step.The yield of nucleic acids and proteins is detected in the third step,and their purity and integrity are verified in the last step.RESULTS The procedure takes as few as 3-4 d from the start to quality verification and is highly efficient.In contrast to the existing kits and reagents,which are primarily based on independent isolation,this RNAzol reagent-based method is characterized by the sequential isolation of RNA,DNA,and proteins from the same cells,and therefore saves time,and has low cost and high efficiency.CONCLUSION The RNA,DNA,and proteins isolated using this method can be used for reverse transcription-polymerase chain reaction,polymerase chain reaction,and western blotting,respectively.

Repetitive administration of cultured human CD34+cells improve adenine-induced kidney injury in mice

BACKGROUND There is no established treatment to impede the progression or restore kidney function in human chronic kidney disease(CKD).AIM To examine the efficacy of cultured human CD34+cells with enhanced proliferating potential in kidney injury in mice.METHODS Human umbilical cord blood(UCB)-derived CD34+cells were incubated for one week in vasculogenic conditioning medium.Vasculogenic culture significantly increased the number of CD34+cells and their ability to form endothelial progenitor cell colony-forming units.Adenineinduced tubulointerstitial injury of the kidney was induced in immunodeficient non-obese diabetic/severe combined immunodeficiency mice,and cultured human UCB-CD34+cells were administered at a dose of 1×106/mouse on days 7,14,and 21 after the start of adenine diet.RESULTS Repetitive administration of cultured UCB-CD34+cells significantly improved the time-course of kidney dysfunction in the cell therapy group compared with that in the control group.Both interstitial fibrosis and tubular damage were significantly reduced in the cell therapy group compared with those in the control group(P<0.01).Microvasculature integrity was significantly preserved(P<0.01)and macrophage infiltration into kidney tissue was dramatically decreased in the cell therapy group compared with those in the control group(P<0.001).CONCLUSION Early intervention using human cultured CD34+cells significantly improved the progression of tubulointerstitial kidney injury.Repetitive administration of cultured human UCB-CD34+cells significantly improved tubulointerstitial damage in adenine-induced kidney injury in mice via vasculoprotective and anti-inflammatory effects.

习近平文化思想:新时代党领导文化建设的科学理论指南与根本行动遵循

习近平文化思想是新时代中国共产党领导文化建设实践的理论升华,构成了习近平新时代中国特色社会主义思想的文化篇。学习贯彻习近平文化思想,需要坚持理论与实践相结合、历史与现实相统一的原则,梳理百余年来中国共产党领导文化建设的脉络,回顾党的十八大以来历次宣传思想工作会议及其理论成果,揭示习近平文化思想正式提出的划时代意义。习近平文化思想涵括了文化的地位作用、前进方向、建设目标、实践路径、发展动力等丰富内涵;彰显了明体达用、体用贯通,与时俱进、开放包容,人民至上、胸怀天下等理论品格;体现了坚定文化自信和文化主体性的极端重要性、“两个结合”的方法准则、新的文化使命等原创性贡献。新时代党领导文化建设在加强党对宣传思想文化工作的领导、培育和践行社会主义核心价值观等方面取得了历史性成就。切实担负起新的文化使命,务必以习近平文化思想为指导,明确新的文化使命的本质要义和精神底蕴,强化理论武装、坚持自信自强、推动文化建设高质量发展,在继续推动文化繁荣、建设文化强国、建设中华民族现代文明的奋斗实践中展现新作为。

习近平文化思想的价值、特质与内在逻辑

习近平文化思想坚持马克思主义的立场观点方法,坚守中华文化立场,是习近平新时代中国特色社会主义思想的文化篇,是中国共产党最新的文化思想成果。习近平文化思想明确了新时代文化建设的路线图和任务书,具有鲜明的实践导向性,为新形势下宣传思想文化工作提供了强大思想武器和科学行动指南。习近平文化思想具有丰富的价值意蕴,丰富和发展了马克思主义文化理论,指明和标注了新时代新的文化使命,也为实现中华民族伟大复兴提供行动指南并注入强大精神动力。习近平文化思想展现出了时代特质,坚守人民立场和政治底色、坚持宏微结合和体用贯通,并彰显了民族特性与世界眼光。习近平文化思想坚持和实现了历史性与现实性的统一,文化本体与文化形态的统一,合规律性与合目的性的统一,内在发展和外在保障的统一。把握习近平文化思想的内在逻辑,有利于深刻认识和理解习近平文化思想生成、存在和发展的内外动因。

“公共文化共同体”的社会价值和时代价值

文章以广州市自2022年起在基层公共文化服务体系建设中逐步开展的试点项目为依据,运用人类命运共同体理念、人类共同价值观和国家治理建设的基本理论,对广州市提出的公共文化共同体新概念、新理念和新模式进行分析,认为公共文化共同体有利于实现从公共文化服务体系到公共文化协作联盟、从公共文化社会参与到公共文化公民参与、从公共文化保障力到公共文化影响力、从公共文化共建共享到公共文化共治共创四个方面的转型升级,有利于体现公共文化权利价值、公共文化共同利益价值、公共文化效能价值和公共文化治理价值,指出公共文化共同体实施的从“公共文化+”向“公共文化×”转型升级是基层公共文化服务体系建设的必然趋势。

习近平文化思想的哲学阐释

习近平文化思想是习近平新时代中国特色社会主义思想的重要组成部分,是新时代中国共产党领导文化建设实践经验的理论概括和科学总结。习近平文化思想深刻体现了新时代中国共产党人文化主体性的守正创新,以“两个结合”为深厚底蕴,坚持民族性与世界性相融通,将“魂脉”与“根脉”相结合,在认识论、历史观和辩证法层面实现了对马克思主义哲学的时代创新。习近平文化思想正面回应了西方文明的现代性困境,聚焦西方现代文明的自反性问题和价值异化倾向,提出构建人类命运共同体与创造人类文明新形态的科学论断。习近平文化思想坚持守正与创新相结合、坚持主体性与多样性相融汇、坚持理论与实践相贯通,在实践中深化了对中国特色社会主义文化建设规律的深刻认识,展现了习近平文化思想蕴含的哲学意蕴,必将在新时代中国特色社会主义文化建设中展现思想伟力,在担当新时代新的文化使命中彰显马克思主义的真理力量。

习近平文化思想形成历程论纲

党的十八大以来,习近平同志站在实现中华民族伟大复兴的战略高度和历史高度,面对世情、国情、党情不断变化的新形势,提出了新时代中国特色社会主义文化建设方面的一系列新思想新观点新论断,形成了习近平文化思想。作为一个不断展开的、开放式的思想体系,习近平文化思想之所以能够具备“明体达用、体用贯通”的理论品格与思想特质,必当有其渊源有自的形成历程。揆诸习近平文化思想形成历程,文化自信塑造了其理论底色,文化发展道路明确了其发展指向,文明交流互鉴彰显了其天下立场,文化强国与中华民族现代文明展现了其宏远目标。习近平文化思想体现了鲜明的实践性与总体性、民族性与时代性、现实性与理想性、包容性与原则性的高度统一,是引领新时代中国特色社会主义文化建设的行动指南。

“公共文化服务共同体”:大文化视野下的现代治理新路径——兼论公共文化设施质效测算模型构建

“公共文化服务共同体”建设是公共文化服务治理体系和治理能力现代化进程中的新产物,是具有中国特色的公共文化现代治理新路径。文章探讨在大文化视野下,公共文化设施的新型样态及其评价标准,提出公共文化设施质效测算模型,从公共文化设施的有效服务供给能力、有效资源保障能力、有效吸引能力和公众满意度四个方面进行测算,以期以统一的、简单的、少人工的指标进行实时动态数据分析,全面展现大文化视野下公共文化服务供给现状和实际质效,从而以科学决策促进“公共文化服务共同体”可持续发展,推动公共文化服务高质量发展。

论习近平文化思想对马克思主义文化理论的继承与发展

习近平文化思想与马克思主义文化理论一脉相承,是马克思主义文化理论的时代表达,实现了对马克思主义文化理论的继承与发展。从马克思主义立场来看,习近平文化思想继承与发展了马克思主义文化理论的唯物主义哲学立场、人民群众政治立场与共产主义价值立场;从马克思主义观点来看,习近平文化思想继承与发展了马克思主义文化理论关于文化认识、文化价值、意识形态文化、民族传统文化与世界文化的观点;从马克思主义方法来看,习近平文化思想继承与发展了马克思主义文化理论的系统分析法、矛盾分析法与唯物辩证法。习近平文化思想内涵丰富、意义重大,开拓了马克思主义文化理论的新境界、开启了中华文化传承与发展的新篇章、开拓了新时代文化强国建设的新征程。

我国红色文化资源数字化建设

红色文化是中国共产党智慧的结晶,是中华优秀传统文化的重要组成部分。在信息化语境下,用数字化手段对红色文化资源进行挖掘、传播和创新,推动红色文化资源的数字化建设,近年来成为热点议题。从文献计量视角出发,运用共词分析和可视化分析方法,以研究趋势、学术团体、关键词共现网络等信息为逻辑分析框架。通过研究发现,我国红色文化资源数字化建设研究共形成了四大热点主题,即新媒体环境下红色文化传播研究、信息化语境下红色文化与高校思政教育融合研究、红色文化资源的数字化传播和传承研究、网络时代下的红色旅游研究。

高校留学生跨文化适应性提升策略分析

在我国教育国际化战略实施进程中,高校留学生教育与管理一直是备受关注的热点和重点,而如何有效提升留学生的跨文化适应性则是令人棘手的难题。高校校园文化与社区文化高度互补,二者对接有助于提升留学生的跨文化适应性。因此,高校不仅要采取措施帮助留学生尽快适应校园生活,还要借助社交媒体APP、跨文化交流活动、社会实践活动、研学旅游、VR技术等工具来帮助留学生获得社会支持、深度融入中国文化环境。这既有利于增强中国高校在国际教育服务贸易中的竞争力,也有利于增进国际的信任与理解。

“互联网+”背景下贵州非遗文创产业发展路径研究

非物质文化遗产是人类历史长河和在人们日常生活中凝聚而成的文化精粹,非遗文化的艺术价值、工艺价值、文化价值以及商业价值等都将在文创设计时焕发新机,而形成产业将会激发出最大的发展潜能。目前,随着信息技术和互联网的飞速发展,贵州非遗的保护、传承以及发展迎来了新契机,而非遗文创产业是对其传承与保护的重要途径,也是推动经济增长的有效方式。本文在“互联网+”背景下分析贵州非遗基本现状及存在的问题,并为贵州非遗文创产业提出发展路径建议,以期促进贵州非遗产业的可持续发展,进而助力贵州经济发展。

实施国家文化数字化战略:站位与定位

本文根据《关于推进实施国家文化数字化战略的意见》明确的战略目标、战略意义和战略框架,分析了文化数字化上升为国家战略的主要原因,将文化大数据的体系框架概括为“两侧四端”,即供给侧、需求侧,以及资源端、生产端、消费端和云端,通过建设文化数据服务中心、搭建文化数据服务平台、创建标识解析体系,实现文化大数据体系云端部署。文化机构只要进入文化大数据体系,就可以共享、交易数据,利用大模型等技术对数据进行深度加工,并将文化数字内容通过云端的文化数据服务中心实现多网多终端分发,最终使中华文化数字化成果的分享路径更加便捷,加快国家文化数字化战略的深入实施。

习近平文化思想的科学性与实践性

党的十八大以来,习近平总书记高度重视党的宣传思想文化工作,主持召开一系列重要会议、发表一系列重要讲话、提出一系列新思想新观点新论断,形成了习近平文化思想。这一思想是一个内涵丰富、逻辑严密的理论体系,科学回答了关于党的宣传思想文化工作的一系列方向性、根本性、战略性的重大问题。这一思想具有深厚的实践基础,新时代十余年来党的宣传思想文化工作在习近平新时代中国特色社会主义思想的指引下,经过“正本清源”与“守正创新”接续努力,取得了历史性成就、发生了历史性变革,也积累了一系列宝贵经验。这一思想正是对党的宣传思想文化工作经验,尤其是新时代实践经验的科学总结。从新时代党的宣传思想文化工作重要经验及其背后逻辑来看,习近平文化思想的科学性和实践性得到进一步彰显和印证,我们党对宣传思想文化工作的规律性认识也进一步深化。

2023设计:技术洪流中的文化追寻

2023年,中国经济提质增效持续推进,人工智能设计相关的工具技术广泛应用,设计在产业领域的作用机制、思维方法、价值影响等进一步向系统化、数据化的方向拓展和深化。关于“人机关系”的忧虑反思,也促使设计中“人”之主体的不可替代性成为人们思辨和探寻的焦点。事实上,在技术工具迭代更新的同时,中国设计在文化向度上也在不断深化,呈现出对传统文化设计更新等“场域”式地综合观照与实践。同时,在社会民生领域,设计作为日常生活善与美的优化路径,表现出精微、质实且深刻的意义。整体上,2023年中国设计在技术工具迭代演进的大趋势中实现了从产业运筹到文化精神追寻与建构的新发展。

习近平文化思想的理论、历史和实践逻辑

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