Effects of WIN 55,212-2 on I_K Current in Cultured Trigeminal Ganglion Neurons of Rat

Summary: To investigate the effects of WIN 55,212-2 on I K in cultured rat trigeminal ganglion (TG) neurons, whole-cell patch clamp techniques were used to record the I K before and after WIN 55,212-2 perfusion at different concentrations. 30 μmol/L WIN 55,212-2 markedly (35 7 %± 7 3 %, P<0.01, n=8) inhibited I K currents, and the currents were partially recovered after washing. 30 μmol/L WIN 55,212-2 also induced a significant depolarizing shift in conductance-voltage parameters (control: V 0 5=10 43 ± 4.25 mV, k=16 27±3 86; WIN 55,212-2: V 0.5=24.71±3.91 mV, k =16.69±2.75; n = 8, P<0.01 for V 0.5). 0.01 μmol/L WIN 55,212-2 slightly (27.0 %± 7.9 %, P<0.05, n=7) increased I K currents, but had no significant change in conductance–voltage parameters (control: V 0.5=10.74±5.27 mV, k=17.33±2.96; WIN 55,212-2: V 0.5=11.06±2.05 mV, k=19.69±6.60; n=7, P>0.05 for V 0.5 and k). These results suggested that WIN 55,212-2 has dual action, which might be through different receptors.

A, ■-Ω METHOD FOR 3-D EDDY CURRENT ANALYSIS

After the field equations and the snonumuoo conditions between the interfaces for 3D eddy current problems Under various gauges were discussed, it was pointed cut in this paper that using the magnetic vector potential A. the electric scalar potential and Coulomb gauge △ .A = 0 in eddy current regions and using the magntetic scalar potential Ω in the non-conducting regions are more suitable. All field equations, the boundary conditions, the interface continuity conditions and the corresponding variational principle of this method are also given

EFFECTS OF THE HOE 694 ON TRANSIENT INWARD CURRENT AND NA^+- CA^(2+) EXCHANGE IN GUINEA PIG CARDIOMYOCYTES

To determine the effects of HOE 694, a new and potent Na＋- H＋ exchanger blocker, on transient inward current (Iti) and Na＋- Ca2＋ exchange during hypoxia- reoxygenation in guinea pig cardiomyocytes. Methods. Cardiomyocytes were isolated from adult guinea pig ventricle. Experiment was performed in an experimental chamber that allowed the cells to be exposed to a sufficiently low O2 pressure. The cells were subjected to hypoxia and reoxygenation. The ionic currents were studied with patch clamp technique. Results. In the absence of HOE 694, hypoxia- reoxygenation induced Iti in 12 of 15 experiments; but in cardiomyocytes pretreated with HOE 694 (10～ 50μ mol/L), the incidence of Iti observed during reoxygenation was reduced to 5 of 11 experiments and 3 of 10 experiments, P Conclusions. Blockade of the Na＋- H＋ exchange by HOE 694 could reduce Ca2＋ overload upon hypoxia- reoxygenation, and inhibition of Na＋- H＋ exchange may also indirectly decrease Na＋- Ca2＋ exchange activity during hypoxia.

Exact Traveling Wave Solutions of Nano-Ionic Solitons and Nano-Ionic Current of MTs Using the exp(-φ (ξ ))-Expansion Method

In this work, the exp(-φ (ξ )) -expansion method is used for the first time to investigate the exact traveling wave solutions involving parameters of nonlinear evolution equations. When these parameters are taken to be special values, the solitary wave solutions are derived from the exact traveling wave solutions. The validity and reliability of the method are tested by its applications to Nano-ionic solitons wave’s propagation along microtubules in living cells and Nano-ionic currents of MTs which play an important role in biology.

Effects of Angiotensin Ⅱ and ACE Inhibitor,Captopril on L-type Calcium Current and Sodium Current of Single Guinea Myocytes

Objectives To investigate effect of Angll, captopril on single guinea myocytes on L - type calcium current and sodium current. Methods Membrane patch clamp whole cell recording technique was used to investigate effect of angll, captopril on L - Ca maximum current density and sodium maximum current density. Resutls Angll increased the maximum current density compared with control after perfused 5 min, 357. 7 ±219. 7 Vs 279. 5± 240. 5 PA/PF, increase rate is 27. 9 %, the shape of current - voltage relationship curve was unchanged, peaked at + 10 mv, indicated that angll increased L - Ca current density in voltage - dependent. After perfused with captopril, captopril + angll 3, 5 min, L - Ca current was recorded, results suggest L - Ca maximum current density decreased significantly compared with control, in captopril group, 128. 4 ± 92. 6Vs286. 2 ± 89. 7, 66. 7±68. 3Vs 286. 2 ± 89. 7, respectively, rate of inhibition is 55. 1 %, 76. 6 %, respectively. L - Ca current further decreased in captopril perfused 5 min compared with 3 min, 66. 7 ± 68. 3 Vs 128. 4 ± 92. 6, in captopril + angll group, L - Ca current decreased greatly in 3, 5 min than control, 143. 4±117. 6Vs 267. 7±141. 4, 96. 4±82. 5 Vs 267. 7±141. 4, respectively, rate of inhibition is 46. 4 % , 63. 9 % respectively. We also investigated effect of captopril on Na current, which decreased significantly in 1 min and 3 min compared with control, 939. 1 ±319. 1 Vs 1398. 0±144. 6 PA/PF, 469. 95 ± 314. 9 Vs 1398. 0 ±144. 6 PA/PF, respectively, rate of inhibition is 32. 8 % , 66. 3 % , respectively. Na current density decreased significantly in 3 min compared with 1 min, 469. 9±314. 9 Vs 939. 1±319. 1PA/PF, rate of inhibition is 49. 9 % . Conclusions Angiotensin Ⅱexerts increased maximum current density of L - Ca in voltage dependent, captopril decreased maximum current density of L - Ca in voltage dependent, decreased sodium maximum current density, which is the prominently antiarrhythmia mechanisms through inhibition of angiotensin Ⅱ evoked calcium dependent transient inward current and calcium overload.

针-板电极空气隙直流放电光信息采集

为研究气体击穿的放电机理,采集直流电压下针-板电极空气隙流注放电击穿瞬间的光信号,实验设计了气体放电光学采集装置.利用光纤,光谱仪和ICCD组成光信号检测系统,其中ICCD单独使用可以捕捉光现象,配合光谱仪可采集光谱.实验中采用具有高速触发脉冲的外部触发方式操作控制器,进行图像及光谱的采集,并对采集到的结果进行分析和讨论.

度量空间中Current的某些特征

作者从代数观念出发研究了Current的表示定理．对于完备不变Current,利用构建的极小三元组技巧,给出了一个类似的Radon-Nikodym定理．

Ionic Conduction and Fuel Cell Performance of Ba0.98Ce0.8Tm0.2O3-α Ceramic

The perovskite-type oxide solid solution Ba0.98Ce0.8Tm0.2O3-α was prepared by high temperature solid-state reaction and its single phase character was confirmed by X-ray diffraction. The conduction property of the sample was investigated by alternating current impedance spectroscopy and gas concentration cell methods under different gases atmospheres in the temperature range of 500-900 ℃. The performance of the hydrogen-air fuel cell using the sample as solid electrolyte was measured. In wet hydrogen, the sample is a pure protonic conductor with the protonic transport number of 1 in the range of 500-600 ℃, a mixed conductor of proton and electron with the protonic transport number of 0.945-0.933 above 600 ℃. In wet air, the sample is a mixed conductor of proton, oxide ion, and electronic hole. The protonic transport numbers are 0.010-0.021, and the oxide ionic transport numbers are 0.471-0.382. In hydrogen-air fuel cell, the sample is a mixed conductor of proton, oxide ion and electron, the ionic transport numbers are 0.942 0.885. The fuel cell using Ba0.98Ce0.8Tm0.2O3-α as solid electrolyte can work stably. At 900 ℃, the maximum power output density is 110,2 mW/cm2, which is higher than that of our previous cell using Ba0.98Ce0.8Tm0.2O3-α （x〈≤1, RE=Y, Eu, Ho） as solid electrolyte.

Effects of alternating current interference on corrosion of X60 pipeline steel

With rapid economic development in China, demand for energy and transportation is growing. Due to the limitations of factors such as terrain and traffic, a large number of buried oil and gas pipelines are parallel to high- voltage transmission lines and electrified railways over long distances. Alternating pipelines is very serious in laboratory experiments were current （AC） corrosion of such cases. In this work, carried out with an electrochemical method in a simulated soil solution at various AC current densities from 0 to 200 A]m2 and AC frequencies from 10 to 200 Hz. Experimental results indicated that with an increase in the AC current density, the corrosion po- tential of an X60 steel electrode shifted negatively, the anodic current density increased significantly, and the corrosion rate increased. Moreover, with an increase in the AC frequency, the corrosion potential of the X60 electrode shifted positively and the anodic current density decreased, which led to a decrease in the corrosion rate. Furthermore, the morphology of X60 electrodes indicated that uniform corrosion occurred at a low AC current density; while corrosion pits were found on the X60 electrode surface at a high AC current density, and deep corrosion pits seriously damaged the pipelines and might lead to leakage.

一种模拟3 V锂电池供电的DC-DC设计

微光像增强器采用3 V锂电池供电,随着电池电压下降,直流工作点变化或调整可能引起其内部高压电源输出异常,导致像管出现闪烁、熄灭或高亮等故障。通常使用具有“电池模拟功能”的直流电源进行供电特性验证,该类设备功率大,功能多,价格也昂贵,像增强器功率小,若采用这种设备则利用率低以至于研发成本高。基于恒流源控制LDO输出,设计一种模拟1.4 V^3.4 V锂电池充放电的DC-DC电路,经仿真和实测验证,输出电压能够线性上升或下降且速率可在数十毫秒至数小时内可调,具备模拟电池供电功能,该电路成本较低,其改进线路还可用于其它领域功率产品作直流扫描分析验证。

On the horizontal distribution of algal-bloom in Chaohu Lake and its formation process

Based on the remote sensing images of algae, the present work analyzes the horizontal distribution characteristics of algal blooms in Chaohu Lake, China, which also reveals the frequency of algal blooms under different wind directions. Further, an unstructured-grid, three-dimensional finite-volume coastal ocean model （FVCOM） is applied to investigate the wind-induced currents and the transport pro- cess to explain the reason why algal blooms occur at the detected places. We first deduce the primary distribution of biomass from overlaid satellite images, and explain the formation mechanism by analyzing the pollution sources, and simulating the flow field and transportation process under prevailing wind over Chaohu Lake. And then, we consider the adjustment action of the wind on the corresponding day and develop a two-time scale approach to describe the whole formation process of algae horizontal distribution in Chaohu Lake. That is, on the longer time scale, i.e., during bloom season, prevailing wind determines the primary distribution of biomass by inducing the characteristic flow field; on the shorter time scale, i.e., on the day when bloom occurs, the wind force adjusts the primary distribution of biomass to form the final distribution of algal bloom.

Formation of epitaxial Tl_2Ba_2Ca_2Cu_3O_(10) superconducting films by dc-magnetron sputtering and triple post-annealing method

For obtaining pure phase T12Ba2Ca2Cu3O10 （T1-2223） films with good superconducting properties, the growth technique is improved by dc magnetron sputtering and a triple post-annealing process. The triple post-annealing process comprises annealing twice in argon and once in oxygen at different temperatures. In the first low-temperature annealing phase in argon, T12Ba2CaCu2O8 （T1-2212） is obtained to effectively minimize evaporation in the next step. With the increase of temperature in the second annealing stage in argon, the previously prepared T1-2212 inter-phase is converted into T1-2223 phase. An additional annealing in oxygen is also adopted to improve the properties of T1-2223 films, each containing an optimal oxygen content value. The results of X-ray diffraction （XRD） θ-2θ scans, 09 scans and rotational φ scans show that each of the T1-2223 films has a high phase purity and an epitaxial structure. Smooth films are observed by scanning electron microscopy （SEM）. The critical temperatures Tc of the films are measured to be about 120 K and the critical current densities Jc can reach 4.0 MA/cm2 at 77 K at self field.

Multi-train modeling and simulation integrated with traction power supply solver using simplified Newton–Raphson method

Multi-train modeling and simulation plays a vital role in railway electrification during operation and planning phase. Study of peak power demand and energy consumed by each traction substation needs to be deter- mined to verify that electrical energy flowing in its railway power feeding system is appropriate or not. Gauss-Seidel, conventional Newton-Raphson, and current injection methods are well-known and widely accepted as a tool for electrical power network solver in DC railway power supply study. In this paper, a simplified Newton-Raphson method has been proposed. The proposed method employs a set of current-balance equations at each electrical node instead of the conventional power-balance equation used in the conventional Newton-Raphson method. This concept can remarkably reduce execution time and computing complexity for multi-train simulation. To evaluate its use, Sukhumvit line of Bangkok transit system （BTS） of Thai- land with 21.6-km line length and 22 passenger stopping stations is set as a test system. The multi-train simulation integrated with the proposed power network solver is developed to simulate 1-h operation service of selected 5-min headway. From the obtained results, the proposed method is more efficient with approximately 18 % faster than the conventional Newton-Raphson method and just over 6 % faster than the current injection method.

Inhibition characteristics of circulating current in parallel inverter driving system of mine hoist

This research investigated the outputting circulation current inhibition characteristic which are controlled by the instantaneous feedback voltage in inverter parallel driving of the mine hoist. We established a transfer function of the parallel inverters controlled by the close-loop adjustment of instantaneous voltage feedback. The influence of the parameters of the close-loop feedback circuit to the inhibition effects to the outputting circulation current is observed. After analyzing the circulating current inhibition characteristics, the proportion integration （PI） controller is introduced into the close-loop adjustment by instantaneous voltage feedback. The characteristics equation is gained to determine the PI parameters by drawing the Bode plots. The inhibition effects of the proposed controller are examined by the established simulation model of parallel inverter system. The harmonic distortion rate at the outputting voltage frequency value of 4, 10, 20, 41 and 50 Hz, are all lower than 2.32 % by the instantaneous outputting voltage feedback.

Tides and short-term variabilities in the Kuroshio west of Yonakuni-jima

Direct current measurements at the mooring station M southwest of Yonakuni-jima are carried out from May 18 to June 1, 1996. The Observed Kuroshio Current at 290 and 594 m depths of the mooring station M is quite steady ddring the pened of Observation. The rotary spectral estimates of the current data by the maximum entropy method show that there are prominent diurnal and semidiurnal spectral peaks. The semidiurnal tide is predominant at 290 m depth while there is the current fluctuation with the inertial period except for the tidal oscillation at 594 m depth. There are also peaks at the pened of 4-7 d. There is a significant coherence between two time series of currents at 290 and 594 m depths in the pened range of 3 - 5 d. The Japan Meteorological Agency (JMA) wind data during the same period as the oceanic measurement are used in comparison with the current meter data. Rotary spectral estimates for the wind data show significant peaks at the period of 3 - 5 d. It is concluded from the cross spectra between the wind and the current that the current fluctuation of 3 - 5 d period at 290 m depth response to the wind fluctuation of the same periods with time lags smaller than 1 d.

LOW TEMPERATURE ALUMINUM FLOATING ELECTROLYSIS IN HEAVY ELECTROLYTE Na_3AlF_6-AlF_3-BaC1_2-NaCl BATH SYSTEM

Multiple regression equations of liquidus temperature, electrical conductivity and bath density of the Na_3AlF_6-AlF_3-BaC1_2-NaCl system were obtained from experiments by using orthogonal regression method. The experiments were carried out in 100A cell with low melting point electrolyte, the influences of cathodic current density, electrolytic temperature, density differences of bath and liquid aluminum on current efficiency (CE) were studied; when the electrolyte cryolite ratio was 2.5, w(BaC1_2) and w(NaCl) were 48% and 10%, respectively, CE reached 90% and specific energy consumption was 10.97k Wb/kg/kg. Because of the fact that aluminum metal obtained floated on the surface of molten electrolyte, this electrolysis method was then defined as low temperature aluminum floating electrolysis. The results showed that the new low temperature aluminum electrolysis process in the Na_3AlF_6-AlF_3-BaC1_2-NaCl bath system was practical and promising.

AC current automatic calibration using two different TCC designs

AC currents are automatically calibrated by two different thermal current converter(TCC)designs.The two designs are different in the used number of the thermal-elements(TEs).Consequently they differ in their output electro motive force(EMF).Studying the effect of changing the output EMF is done in this paper through calibrating AC currents.5 mA and 5 A are accurately calibrated at different frequencies 55 Hz,1 kHz and10 kHz by the two TCCs.A comparison is made between the results to evaluate the effect of the output EMF value on the accuracy and the uncertainty of the low and higher AC current calibration.A LabVIEW program is designed for this accurate automatic calibration to overcome the problems of the manual calibration on the thermal converters.

Solar Flare Activity and Variability of Electric Current Helicity

Recent study using Huairou vector magnetograph data shows that durlug flare activity, the current helicity exhibits rapid and substantial variations and, in some cases, a recovering phase. We considered various representative the magnetic configurations. First, interacting twisted magnetic flux tubes are analyzed separately for positive, negative and mixed-sign helicity regions. The results show that the helicity during flares decreases in positive-sign, and increases in negative-sign regions. Then, flaring arcade also shows that the magnitude of the helicity decreases after flares. We also investigated current sheets formed by sheared magnetic field and showed that the current helicity (with either positive and negative signs) vanishes after reconnection. The emergence of twisted flux tubes which can trigger flares may be another source of flare-associated variability of current helicity. We demonstrate the relevance of current helicity to the description of flare activity by comparing its variation with that of shear angle in the active region AR 6891.

Observational Features of Large-Scale Structures as Revealed by the Catastrophe Model of Solar Eruptions

Large-scale magnetic structures are the main carrier of major eruptions in the solar atmosphere. These structures are rooted in the photosphere and are driven by the unceasing motion of the photospheric material through a series of equilibrium configurations. The motion brings energy into the coronal magnetic field until the system ceases to be in equilibrium. The catastrophe theory for solar eruptions indicates that loss of mechanical equilibrium constitutes the main trigger mechanism of major eruptions, usually shown up as solar flares, eruptive prominences, and coronal mass ejections （CMEs）. Magnetic reconnection which takes place at the very beginning of the eruption as a result of plasma instabilities/turbulence inside the current sheet, converts magnetic energy into heating and kinetic energy that are responsible for solar flares, and for accelerating both plasma ejecta （flows and CMEs） and energetic particles. Various manifestations are thus related to one another, and the physics behind these relationships is catastrophe and magnetic reconnection. This work reports on recent progress in both theoretical research and observations on eruptive phenomena showing the above manifestations. We start by displaying the properties of large-scale structures in the corona and the related magnetic fields prior to an eruption, and show various morphological features of the disrupting magnetic fields. Then, in the framework of the catastrophe theory, we look into the physics behind those features investigated in a succession of previous works, and discuss the approaches they used.

基于漏电流处理的高灵敏度多碱光电阴极制作

高灵敏度多碱光电阴极是决定超二代像增强器性能的关键因素。但在真空蒸发制作中,钾、钠蒸发经常出现漏电流过大,轻则降低了多碱光电阴极的灵敏度,重则导致不能形成多碱光电阴极结构。因之,本文从实际工艺流程出发,分析了产生漏电流的可能原因,试验量化对比了漏电流处理与否对多碱光电阴极灵敏度及其超二代像增强器成像质量的影响程度,提出了一种K-Na-Sb循环漏电流处理策略。基于K-Na-Sb循环漏电流处理的高灵敏度多碱光电阴极,有利于提升超二代像增强器的使用性能和经济效益。