Effects of sodium ferulate on the ultrarapid delayed rectifier K^+ current in human atrial myocytes

Objective：To study the effects of sodium ferulate on the ultrarapid delayed rectifier K^＋ current（IKur） in human atrial myocytes. Methods：Human atrial myocytes were isolated by enzyme dispersion method. IKur, in human atrial myocytes were recorded by using the whole cell patch clamp. The changes of IKur were compared in the absence and the presence of sodium ferulate. Results：There was no effect of 0.4 g/L sodium ferulate on I-V relation of IKur. However, 0.4 g/L sodium ferulate inhibited IKur to some degrees at each test pulse. The current densities of IKur at ＋60 mV decreased from 4.997 ± 0.35 PA/PF to 3.331 ± 0.26 PA/PF（n = 6, P 〈 0.05）. The inhibitory effect was concentration-dependent. IC50 was（0.41 ±0.03）g/L and the Hill coefficient was 0.95 ± 0.05. Conclusion：Sodium ferulate as a potassium channel blocker can inhibit IKur in human atrial myocytes effectively.

Effects of Atractylodes Macrocephala on the Cytomembrane Ca^(2+)-activated K^+ Currents in Cells of Human Pregnant Myometrial Smooth Muscles

The study examined the inhibitory effect of Atractylodes macrocephala （AM） on the uterine contraction during premature delivery and explored its electrophysiological mechanism by studying the effects of AM on the Ca^2＋-activated K^＋ currents of pregnant human myometrial smooth muscle cells with or without the treatment with intedeukin-6. Single cells were acutely isolated from pregnant human myometrial smooth muscles. Whole-cell Ca^2＋-activated K^＋ currents were recorded by using an Axopatchl-D amplifier. The cells were divided into three groups： group A in which AM was added into perfusate, group B, in which interleukin-6 was added into perfusate） and group C in which AM was added into perfusate after addition of interleukin-6. IL-6 10 ng/mL inhibited BKca by 36.9%±13.7% as compared with control （P〈0.01）. AM at 2 mg/mL raised BKca by 36.7%±22.6% or 45.2%±13.7% with or without the treatment of IL-6, respectively （P〈0.01）. It is concluded that AM was able to enhance the BKca of pregnant human myometrial smooth muscle cells treated or untreated with interleukin-6 and its effect on the BKca IL-treated cells was stronger that its effect on BKca of untreated cells. Our results suggested that AM can help to maintain the membrane potentials and the resting status of pregnant human myometrial smooth muscle cells.

Action Mechanism Research of Lanthanons to Slow Vacuolar Ion Channels in Raphanus Satirus L.(Xinlimei) Radish by Patch-Clamp

We used whole-vacuolar patch-clamp recording mode to study the action mechanism of La3+ to Slow Vacuolar (SV) channels for the first time. We recorded SV channel currents of Xinlimei (Raphanus satirus L.) vacuolars. The minimum activation potentials of voltage-dependent SV channels tied in 25+/-5 mV. The increase in cytoplasmic Ca2+ led to enhancement of SV-type currents. It was found that the threshold potential of activation shifted towards more depolarized values whenever cytoplasmic Ca2+ was increased. When 10(-10) mol/L free La3+ was added to the bath, SV-type current was suppressed by 60 similar to 75%. These data showed La3+ reduced ion permeabilities of Xinlimei root vacuolar membrane.

Protective Effect of Carvedilol on Abnormality of L-type Calcium Current Induced by Oxygen Free Radical in Cardiomyocytes

The protective effect of carvedilol on abnormality of L-type calcium current induced by oxygen free radical in single guinea pig ventricular myocytes was studied. Whole-cell patch clamp technique was used to study the effect of H 2O 2 (0.5 mmol/L) on L-type calcium current in single guinea pig ventricular myocytes and the action of pretreatment with carvedilol (0.5 μmol/L). 0.5 μmol/L carvedilol had no significant effect on I Ca,L and its channel dynamics. In the presence of 0.5 mmol/L H 2O 2, peak current of I Ca,L was reduced significantly (P<0.001), the I-V curve of I Ca,L was shifted upward, steady-state activation curve and steady-state deactivation curve of I Ca,L were shifted left and recovery time of I Ca,L was delayed significantly (P<0.001). 0.5 μmol/L carvedilol significantly alleviated the inhibitory effect of H 2O 2 on I Ca,L as compared with that in H 2O 2 group (P<0.01). In addition, carvedilol reversed the changes of dynamics of I Ca,L induced by H 2O 2. It was concluded that carvedilol could alleviate the abnormality of L-type calcium current induced by oxygen free radical in cardiomyocytes. It shows partly the possible mechanism of the special availability of carvedilol in chronic heart failure.

The Changes of Potassium Currents in Rabbit Ventricle with Healed Myocardial Infarction

To elucidate the mechanism of arrhythmia in healed myocardial infarction (HMI), the changes of action potential duration (APD), transient outward potassium current (I to), delayed rectifier potassium current (I K) and inward rectifier potassium current (I K1) of left ventricular myocytes in non-infarcted zone of HMI were investigated. Rabbits were randomly assigned into two groups: HMI group, in which animals were subjected to thoracotomy and ligation of the circumflex coronary and sham-operated group, in which rabbits underwent thoracotomy but no conorary ligation. 3 months after the operation, the whole myocyte patch clamp technique was used to record APD, I to, I K, and I K1 of ventricular myocytes in non-infarcted zone.Our results showed that the membrane capacitance was larger in HMI group than in sham-operated group. Action potential duration was significantly lengthened in HMI group and early afterdepolarization (EAD) appeared in HMI group. The densities of I to, I K, tail, and I K1 were reduced significantly in HMI group, from 6.72±0.42 pA/pF, 1.54±0.13 pA/pF and 25.6±2.6 pA/pF in sham-operated group to 4.03±0.33 pA/pF, 1.14±0.11 pA/pF and 17.6 ±2.3 pA/pF, respectively. It is concluded that the reduced densities of I to , I K, tail and I K1 in ventricular myocytes of non-infarcted zone in HMI were responsible for the prolongation of APD and the presentation of EAD which played important roles in the development of malignant arrhythmia in HMI.

The Effect of Extrogenous Phosphocreatine on L-type Calcium Current in Ischemic Guinea Pig Ventricular Myocytes

Objectives Heart failure （HF） is one of the most common outcome for all kinds of heart diseases, the effects of energetic therapy on HF remains controversial, especially to ischemic HF. The aim of this study was to explore the effect of exogenous phosphocreatine with different concentration on L-type calcium（I Cc-L） current in ischemic ventricular myocytes of guinea pig and to investigate its underlying electrophysiological mechanism for the treatment of ischemic HF. Methods Single ventricular myocytes were isolated enzymatically from left ventricle of guinea pig. Peak I Ca-L current were recorded using patch clamp techniques in the whole-cell configuration when myocytes had been superfused with normal Tyrode solution, simple ischemic solution, ischemic solution containing phosphocreatine with different concentration for 10 minutes respectively. Results Peak I Ca-L current density of myocytes superfused with simple simulated ischemic solution was remarkably inhibited by 80.6 ± 5.2% compared with myocytes superfused with normal Tyrode solution（P〈0.05）. Ischemic solution containing phosphocreatine of 5, 10, 20, 30mmol/L inhibited Peak I Ca-L current density by （53.8±6.7）%, （41.8 ± 8.2）%, （38.1±7.4）%, （36.6±9.7）% respectively. There was no statistical significance among phosphocreation of 10, 20, 30 mmol / L. Conclusions Extrogenous phosphocreatine could reverse the inhibition of I Ca-L current under ischemic condition, which could be the ionic basis for the treatment of ischemic heart failure. 0-10 mmol/L phosphocreatine exerted significant dose-effect relationship which no longer existed as concentration more than 10 mmol/L. It is supposed that phosphocreatine increased I Ca-L current by many pathways rather than simple substrate for ATP synthesis.

CHARACTERISTICS OF ACTIONPOTENTIAL AND THEIR UNDERLYING OUTWARD CURRENTS IN MAMMALIAN TASTE RECEPTOR CELLS

Many rat taste receptor cells conduct action potentials(APs).APs had a mean threshold of -35 mV(n=95 cells)and a spike height of 52mV above threshold in current clamp(hold= -80mV).Aps could be classified into two significantly different (P<0.001) groups-fast,with short half-time durations and large outward currents (mean1.3 ms and 2.7nA),and slow,with long duration and small outward currents(mean9.2ms and 0. 29nA).AP upstrokes were conducted by TTX-sensitive sodium currents whereas the downstroke by TEA-blockable outward currents. Voltage dependent analysis of outward current separated transient and sustained components.The transient component was specifically blocked by 4-AP(1mmol/L).A calcium-dependent outward component was also revealed modulating voltage and external calcium concentration.The fast recovery phase of the AP appears related the sustained outward current whereas the after hyperpolarization(AHP) was blocked by 4AP suggesting a significant contribution of the transient component.Forskolin (FSK),which elevates cAMP,reversibly blocked the majority of the sustained current without influencing the transient. FSK greatly exaggerated the AHP without changing the spike height or duration. These data suggest that several components of the outward current contribute specifically to the gustatory AP and that the AP may be modulated by cyclic nucleotides.

Effects of Angiotensin Ⅱ and ACE Inhibitor,Captopril on L-type Calcium Current and Sodium Current of Single Guinea Myocytes

Objectives To investigate effect of Angll, captopril on single guinea myocytes on L - type calcium current and sodium current. Methods Membrane patch clamp whole cell recording technique was used to investigate effect of angll, captopril on L - Ca maximum current density and sodium maximum current density. Resutls Angll increased the maximum current density compared with control after perfused 5 min, 357. 7 ±219. 7 Vs 279. 5± 240. 5 PA/PF, increase rate is 27. 9 %, the shape of current - voltage relationship curve was unchanged, peaked at + 10 mv, indicated that angll increased L - Ca current density in voltage - dependent. After perfused with captopril, captopril + angll 3, 5 min, L - Ca current was recorded, results suggest L - Ca maximum current density decreased significantly compared with control, in captopril group, 128. 4 ± 92. 6Vs286. 2 ± 89. 7, 66. 7±68. 3Vs 286. 2 ± 89. 7, respectively, rate of inhibition is 55. 1 %, 76. 6 %, respectively. L - Ca current further decreased in captopril perfused 5 min compared with 3 min, 66. 7 ± 68. 3 Vs 128. 4 ± 92. 6, in captopril + angll group, L - Ca current decreased greatly in 3, 5 min than control, 143. 4±117. 6Vs 267. 7±141. 4, 96. 4±82. 5 Vs 267. 7±141. 4, respectively, rate of inhibition is 46. 4 % , 63. 9 % respectively. We also investigated effect of captopril on Na current, which decreased significantly in 1 min and 3 min compared with control, 939. 1 ±319. 1 Vs 1398. 0±144. 6 PA/PF, 469. 95 ± 314. 9 Vs 1398. 0 ±144. 6 PA/PF, respectively, rate of inhibition is 32. 8 % , 66. 3 % , respectively. Na current density decreased significantly in 3 min compared with 1 min, 469. 9±314. 9 Vs 939. 1±319. 1PA/PF, rate of inhibition is 49. 9 % . Conclusions Angiotensin Ⅱexerts increased maximum current density of L - Ca in voltage dependent, captopril decreased maximum current density of L - Ca in voltage dependent, decreased sodium maximum current density, which is the prominently antiarrhythmia mechanisms through inhibition of angiotensin Ⅱ evoked calcium dependent transient inward current and calcium overload.

Sex Difference in the Repolarization Currents of Rabbit Ventricular Cells

The current difference between male and female rabbit ventricular myocytes was investigated for elucidating the mechanism of longer QT interval and higher incidence of drug-associated torsade de pointes in female rabbits than in male rabbits. Whole cell patch clamp technique was used to record APD, I_to, I_K,tail, I_K1 and I_Ca,L of myocytes from left ventricular apex. There was no difference in the membrane capacitance between male and female rabbit myocytes. APD_90 was longer in female rabbits (560.4±26.5 ms, n=15) than in male ones (489.0±20.7 ms, n=14), P<0.05. In female rabbit myocytes, I_K,tail, I_to, I_K1 and I_Ca,L were 0.71±0.05 pA/pF (n=17), 8.28±1.03 pA/pF (n=18), 24.5±3.6 pA/pF (n=12) and 9.0±2.3 pA/pF (n=15) respectively. In male rabbit myocytes, they were 0.84±0.07 pA/pF (n=18), 8.60±1.20 pA/pF (n=18), 25.9±4.5 pA/pF (n=14) and 9.3±2.6 pA/pF (n=16) respectively. I_K,tail in female rabbits was significantly lower than that of male rabbits (P<0.05), but there was no difference in I_to, I_K1 and I_Ca,L between male rabbits and female rabbits (P>0.05). The lower I_K,tail of female rabbit myocytes may contribute to the longer repolarization and the higher incidence of drug-associated torsade de pointes.

马钱子碱抑制豚鼠心室肌细胞钠电流的作用研究

目的本研究旨在分析马钱子碱(brucine)对分离的单个豚鼠心室肌细胞钠电流(INa+)的作用,并探索其抗心律失常的可能机制。方法共选取24只健康的成年豚鼠作为实验对象,雌雄不拘,随机分为4组,每组6只:第1组是未接受任何处理的对照组,之后通过微量加样器使培养皿中brucine药物的终浓度分别达到3μmol/L、10μmol/L、30μmol/L。采用急性酶解法分离获得单个豚鼠心室肌细胞,通过全细胞膜片钳技术测量离子通道电流,以检测不同浓度的brucine对豚鼠心室肌细胞INa+的影响。结果正常的对照组没有明显的INa+电流峰值的变化,而brucine 3μmol/L组给药前后基本不影响INa+电流峰值,当brucine的浓度增加到10μmol/L时,给药前后INa+电流峰值显著下降(P<0.05),在30μmol/L组给药前后INa+电流峰值大幅度减少(P<0.01),并且这种抑制作用呈浓度依赖性。正常对照组及brucine 3μmol/L剂量组中,电流-电压曲线(Ⅰ~Ⅴ曲线)并未受到显著的影响;然而,10μmol/L、30μmol/L剂量组与对照组比较,INa+的Ⅰ~Ⅴ曲线上移,无平行移动,且曲线形状不变。结论Brucine能够通过抑制心室肌细胞钠通道电流发挥抗心律失常作用。

三电平辅助变流器寿命优化控制

辅助变流系统是动车组车辆上关键的电气组成部分,主要为空调机组、风机、照明等交流负载提供稳定的三相电源。结温是影响动车组三电平辅助变流器IGBT(Insulated-gate Bipolar Transistor)模块寿命的主要因素。为了提高动车组三电平中点钳位(Neutral-Point Clamped,NPC)型辅助变流器寿命,提出一种降低结温的模型预测电流控制(Model Predictive Current Control,MPCC)策略。首先,考虑传统MPCC在每个采样周期需要代入27个电压矢量循环计算,计算量较大,因此将三电平基本电压矢量划分在间隔60°的6个扇区中,通过计算电压矢量在两相静止坐标下的相角,进而判断参考电压矢量所处扇区,将备选矢量数目从27缩减至10,减少了计算量;其次,传统MPCC直接应用于动车组辅助变流器会使IGBT模块的结温较高,老化速度加快,对变流器安全稳定运行产生不可预测的损害,本文对IGBT模块的开关损耗和导通损耗进行近似等效,得到了和集射极电压、集电极电流相关的功率损耗因子。通过预测每相电流的方向,进而预测IGBT模块的开关和导通情况,动态加入功率损耗因子,并在代价函数中约束每相IGBT及其续流二极管(Free Wheeling Diode,FWD)的功率损耗,使得最优电压矢量在降低功率损耗的同时保证一定的控制性能。通过仿真与实验验证,本文所提方法相比传统MPCC策略降低了功率器件的结温,提高了变流器寿命。

谐振方式对有源钳位反激变换器工作状态影响的研究

传统有源钳位反激变换器依赖变压器漏感和原边钳位电容之间的谐振实现软开关,消除了开关损耗,但传导损耗大,输出整流器存在电流振荡现象。为解决上述问题,选取变换器最佳工作模式,建立不同谐振方式下的等效电路模型,探究不同谐振方式对变换器性能的影响,以副边谐振方式为基础制作实验样机。由实验结果可知:副边谐振方式有源钳位反激变换器可实现功率器件软开关,降低原边电流有效值,消除副边电流振荡现象,所设计的变换器最大满载效率达94.06%,整机效率高于原边谐振方式。

Protein Kinase C Enhances the Swelling-Induced Chloride Current in Human Atrial Myocytes

Swelling-activated chloride currents（ICl.swell） are thought to play a role in several physiologic and pathophysiologic processes and thus represent a target for therapeutic approaches. However, the mechanism of ICl.swell regulation remains unclear. In this study, we used the whole-cell patch-clamp technique to examine the role of protein kinase C（PKC） in the regulation of ICl.swell in human atrial myocytes. Atrial myocytes were isolated from the right atrial appendages of patients undergoing coronary artery bypass and enzymatically dissociated. ICl.swell was evoked in hypotonic solution and recorded using the whole-cell patch-clamp technique. The PKC agonist phorbol dibutyrate（PDBu） enhanced ICl.swellin a concentration-dependent manner, which was reversed in isotonic solution and by a chloride current inhibitor, 9-anthracenecarboxylicacid. Furthermore, the PKC inhibitor bis-indolylmaleimide attenuated the effect and 4α-PDBu, an inactive PDBu analog, had no effect on ICl.swell. These results, obtained using the whole-cell patch-clamp technique, demonstrate the ability of PKC to activate ICl,swell in human atrial myocytes. This observation was consistent with a previous study using a single-channel patch-clamp technique, but differed from some findings in other species.

Two outward potassium current types are expressed during the neural differentiation of neural stem cells

The electrophysiological properties of potassium ion channels are regarded as a basic index for determining the functional differentiation of neural stem cells. In this study, neural stem cells from the hippocampus of newborn rats were induced to differentiate with neurotrophic growth factor, and the electrophysiological properties of the voltage-gated potassium ion channels were observed. Immunofluorescence staining showed that the rapidly proliferating neural stem cells formed spheres in vitro that expressed high levels of nestin. The differentiated neurons were shown to express neuron-specific enolase. Flow cytometric analysis revealed that the neural stem cells were actively dividing and the percentage of cells in the S ＋ G2/M phase was high. However, the ratio of cells in the S ＋ G2/M phase decreased obviously as differentiation proceeded. Whole-cell patch-clamp re- cordings revealed apparent changes in potassium ion currents as the neurons differentiated. The potassium ion currents consisted of one transient outward potassium ion current and one delayed rectifier potassium ion current, which were blocked by 4-aminopyridine and tetraethylammonium, respectively. The experimental findings indicate that neural stem cells from newborn rat hippo- campus could be cultured and induced to differentiate into functional neurons under defined condi- tions in vitro. The differentiated neurons expressed two types of outward potassium ion cur＇ents similar to those of mature neurons in vivo.

电容电压平衡的四电平有源中性点钳位逆变器的改进模型预测电流控制

多电平有源中点钳位(ANPC)逆变器具有功率器件电压应力小、电流谐波低和器件损耗可调控等优点,被广泛应用于工业驱动中。针对四电平ANPC(4L-ANPC)逆变器的控制与调制研究相对较少,现有的调制方案实现复杂,中点电压(NPV)控制难度较大。因此,该文针对三相4L-ANPC逆变器,提出一种改进的有限集模型预测电流控制(FS-MPCC)算法,避免了复杂的调制过程。首先,使用仅包含电流误差的代价函数对位于六边形顶点的6个大电压矢量进行寻优;其次,对参与第一步寻优的大矢量划分扇区,使用包含电流误差和电容电压误差的代价函数对最优大矢量所在扇区的所有电压矢量滚动寻优,得到目标电压矢量并作用于逆变器;最后,仿真和实验结果表明,与传统的FS-MPCC相比,所提方法在降低系统计算负担的同时,还具有较好的控制性能和电容电压平衡能力。

环状RNA mmu_circ_0005019影响小鼠心肌细胞钙激活钾通道电流及动作电位

目的 探索环状RNA mmu_circ_0005019调控小电导钙激活钾(small-conductance calcium-activated potassium, SK)通道蛋白亚基SK3编码基因Kcnn3的表达,以及对小电导钙激活钾通道电流(IK,Ca)和动作电位时程(action potential duration, APD)的影响。方法 在小鼠HL-1细胞中分别构建mmu_circ_0005019过表达和干扰模型,分为过表达组(n=3)、空质粒组(n=3)、干扰1组(n=3)、干扰2组(n=3)、对照组(n=3),通过RT-qPCR、Western blot分析mmu_circ_0005019调节Kcnn3表达的分子机制,应用膜片钳技术电流钳模式记录全细胞的IK,Ca,并用电压钳模式记录APD。结果 成功构建了环状RNA mmu_circ_0005019过表达和干扰的HL-1细胞模型。过表达组的Kcnn3基因表达与空质粒组相比明显上调(P<0.05);干扰1组和干扰2组的Kcnn3基因表达与对照组相比均明显下调(P<0.05)。电生理发现过表达mmu_circ_0005019增加了HL-1细胞IK,Ca电流密度,APD显著缩短;相反,干扰mmu_circ_0005019减少了IK,Ca电流密度,APD显著延长。结论 mmu_circ_0005019可以上调小鼠HL-1细胞Kcnn3的表达水平,从而改变IK,Ca和APD,提示环状RNA mmu_circ_0005019可能在房颤发生中起到促进作用。

紫云英苷对SD大鼠心肌细胞瞬时外向钾通道电流的影响

为探究紫云英苷(astragalin,AG)对心肌细胞瞬时外向钾通道电流Ito的作用,用langendorff主动脉逆向灌流法急性分离出单个SD大鼠心肌细胞,进行全细胞膜片钳试验,记录给药前后Ito的变化及激活、失活和复活过程中的动力学特征。结果表明:不同浓度的AG均表现出对Ito的抑制作用,在50、100、200μmol·L^(-1)浓度范围内,随着药物浓度的增加,抑制作用逐渐增强。以给药前Ito幅值为参照,给予各浓度AG后,电流分别降至原来的(84.06±2.75)%、(66.09±4.65)%、(48.79±5.96)%,后2个浓度的效应与给药前相比具有统计学意义。在动力学特征方面,50、100、200μmol·L^(-1)浓度的AG使瞬时外向钾通道激活阈值上升,失活加速,从失活状态到活化状态的转换延长。综上,AG对SD大鼠心肌细胞上的Ito有抑制作用,此效应的产生与通道的激活、失活和失活后恢复动力学过程改变有关。

换流变压器铁心夹件接地电流谐波特性分析

为了解决仅依据接地电流的全电流值无法准确有效判断换流变压器的检修状态问题,以及更深入探究换流变压器铁心、夹件接地电流的特性,采用汉宁窗插值快速傅里叶变换算法对采样信号进行谐波分析,得到接地电流基波和2~50次谐波的幅值及含有率。对比分析基波与全电流的变化趋势,以及不同时刻接地电流的全电流、基波、谐波数据,得到谐波幅值变化幅度,进一步揭示换流变压器铁心、夹件接地电流值过大和跳动的问题,为判断换流变压器铁心、夹件接地电流状态与变压器运行状况提供新的思路。

IGCT过应力关断下动态雪崩诱发的电压自箝位效应

作为高压功率器件,集成门极换流晶闸管(IGCT)关断过程中必然发生的动态雪崩效应是限制器件反偏安全工作区(RBSOA)的重要因素。通过建立能更好地反映电流集中效应的多单元仿真结构模型,对IGCT过应力条件下的关断特性进行了仿真,发现了一种与开关自箝位模式(SSCM)非常相似的电压自箝位效应。然而,与SSCM不同的是,这种自箝位效应是由动态雪崩效应诱发的。在强烈的动态雪崩下,雪崩产生的载流子与被移除的载流子达到了动态平衡,耗尽区电场无法展宽导致了电压箝位。由于自箝位期间雪崩产生的电流丝无法快速移动,器件极易因局部过热而损毁。并且,随着器件电流增益的增大,这种效应更容易发生。研究结果可为IGCT提供更清晰的RBSOA。

基于相电流查表法的工业机器人用PMSM逆变器非线性补偿策略

针对传统方法无法完全考虑所有逆变器非线性因素的问题,提出一种基于相电流预估值查表法的误差电压精确计算方法。在综合考虑逆变器关断/开通延时、死区时间、寄生电容等多种非线性因素的影响的基础上,采用滤波后的电流指令进行误差电压查表,有效避免反馈电流引入谐波、电流钳位等干扰因素对查表法逆变器非线性补偿策略的影响。通过搭建伺服电机对托平台,验证了所提方法在工业机器人控制中的有效性。实验结果表明:与传统逆变器非线性补偿策略相比,所提算法具有更优秀的电流谐波抑制能力,可实现更优异的补偿效果,补偿后相电流THD小于1.5%。