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Generation and characterization of expressed sequence tags(ESTs) from coralloid root cDNA library of Cycas debaoensis 被引量:1
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作者 Yunhua Wang Nan Li +1 位作者 Ting Chen Yiqing Gong 《Plant Diversity》 SCIE CAS CSCD 2018年第5期245-252,共8页
A normalized full-length cDNA library was constructed from the coralloid roots of Cycas debaoensis by the DSN (duplex-specific nuclease) normalization method combined with the SMART (Switching Mechanism At 5' end ... A normalized full-length cDNA library was constructed from the coralloid roots of Cycas debaoensis by the DSN (duplex-specific nuclease) normalization method combined with the SMART (Switching Mechanism At 5' end of the RNA Transcript) technique. The titer of the original cDNA library was about 1.5 × 10^6 cfu·mL^-1 and the average insertion size was about 1 kb with a high recombination rate (97%). The 5011 high-quality expressed sequence tags (ESTs) were obtained from 5393 randomly picked cDNA clones. Clustering and assembly of ESTs resulted in 2984 unique sequences, consisting of 618 contigs and 2366 singlets. EST sequence annotation revealed that 2333 and 1901 unigenes were functionally anno- tated in the NCBI non-redundant database and Swiss-Prot protein database, respectively. Functional analysis demonstrated that 1495 (50.1%) unigenes were associated with 4082 Gene Ontology (GO) terms. A total of 847 unigenes were grouped into 22 Cluster of Orthologous Groups (COG) functional categories. Based on the EST dataset, 22 ESTs that encoded putative receptor-like protein kinase (RLK) genes were screened. Furthermore, a total of 94 simple sequence repeats (SSRs) were discovered, of which 20 loci were successfully amplified in C debaoensis. This study is the first EST analysis for the coralloid roots of C debaoensis and provides a valuable genomic resource for novel gene discovery, gene expression and comparative genomics, conservation and management studies as well as applications in C debaoensis and related cycad species. 展开更多
关键词 cycas debaoensis Coralloid root cDNA library Expressed sequence tags Symbiosis and defense SSRS
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德保苏铁幼苗和成年植株的光合特性
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作者 潘李泼 陈泰国 +3 位作者 朱舒靖 韦霄 邹蓉 唐健民 《中南农业科技》 2024年第7期54-57,共4页
通过研究德保苏铁(Cycas debaoensis)幼苗和成年植株的光合特性,为其种质资源保育及引种栽培提供理论依据。以德保苏铁幼苗和成年植株为材料,用Li-6400型便携式光合仪器测定其叶片的净光合速率、气孔导度、蒸腾速率等光合生理指标,并绘... 通过研究德保苏铁(Cycas debaoensis)幼苗和成年植株的光合特性,为其种质资源保育及引种栽培提供理论依据。以德保苏铁幼苗和成年植株为材料,用Li-6400型便携式光合仪器测定其叶片的净光合速率、气孔导度、蒸腾速率等光合生理指标,并绘制在一定的光照辐射强度梯度下的光响应曲线。结果表明,德保苏铁在不同时期对强光和弱光都有较高的利用能力;德保苏铁在幼苗阶段对弱光有更强的转化利用能力。日变化中,幼苗净光合速率与气孔导度、胞间CO_(2)浓度、蒸腾速率呈负相关,与光强呈正相关;成年植株净光合速率与气孔导度、蒸腾速率、光强呈负相关,与胞间CO_(2)浓度呈正相关。 展开更多
关键词 德保苏铁(cycas debaoensis) 光响应曲线 幼苗 成年植株 光合特性
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