CTB(cell to body)电池车身一体化技术在提升续航里程、整车刚度和耐撞性等方面具有很大优势,已成为新能源汽车行业发展新方向,但要将电池上盖与车身地板二合为一,密封是限制CTB技术发展的最大难题之一,目前行业在CTB密封领域的研究还...CTB(cell to body)电池车身一体化技术在提升续航里程、整车刚度和耐撞性等方面具有很大优势,已成为新能源汽车行业发展新方向,但要将电池上盖与车身地板二合为一,密封是限制CTB技术发展的最大难题之一,目前行业在CTB密封领域的研究还是空白。本文从CTB密封策略、密封结构设计、密封组件选型、失效后果分析和用户工况设计验证展开研究,首次提出攻克行业内CTB密封设计难题的解决方案,加速CTB技术普及应用,推动全球新能源汽车产业电动化转型。展开更多
The effects of leptin on cytotrophoblast proliferation and invasion activity were investigated. Immunohistochemistry was used to determine the placental expression of leptin in first-trimester pregnancy. By using RT-P...The effects of leptin on cytotrophoblast proliferation and invasion activity were investigated. Immunohistochemistry was used to determine the placental expression of leptin in first-trimester pregnancy. By using RT-PCR and quantitative real-time PCR, the expression of leptin in cytotrophoblast and the effect of leptin on cytotrophoblast secretion were detected. The potential of cell proliferation, invasiveness and migration was assessed by MTT, Transwell invasion assay and migration assay respectively when the cytotrophoblast was cultured with different concentrations of leptin. The results showed that： （1） Leptin was distributed diffusely around cell membrane, in cytoplasma, and on nuclear mem- brane of cytotrophoblast; （2） Leptin mRNA was expressed in cytotrophoblast. Ten ng/mL leptin could promote the secretion of cytotrophoblast significantly （P〈0.01）; （3） After culture with different concentrations of leptin for 24 h or longer, the proliferation of cytotrophoblast was inhibited, while in 24 h leptin could promote cytotrophoblast invasion and migration. Leptin at a concentration of 500 ng/mL could promote cytotrophoblast invasiveness and migration significantly as compared with controls （P〈0.05）. It was suggested that leptin could inhibit cytotrophoblast proliferation, and promote cytotro- phoblast invasion and migration activity.展开更多
文摘CTB(cell to body)电池车身一体化技术在提升续航里程、整车刚度和耐撞性等方面具有很大优势,已成为新能源汽车行业发展新方向,但要将电池上盖与车身地板二合为一,密封是限制CTB技术发展的最大难题之一,目前行业在CTB密封领域的研究还是空白。本文从CTB密封策略、密封结构设计、密封组件选型、失效后果分析和用户工况设计验证展开研究,首次提出攻克行业内CTB密封设计难题的解决方案,加速CTB技术普及应用,推动全球新能源汽车产业电动化转型。
文摘The effects of leptin on cytotrophoblast proliferation and invasion activity were investigated. Immunohistochemistry was used to determine the placental expression of leptin in first-trimester pregnancy. By using RT-PCR and quantitative real-time PCR, the expression of leptin in cytotrophoblast and the effect of leptin on cytotrophoblast secretion were detected. The potential of cell proliferation, invasiveness and migration was assessed by MTT, Transwell invasion assay and migration assay respectively when the cytotrophoblast was cultured with different concentrations of leptin. The results showed that： （1） Leptin was distributed diffusely around cell membrane, in cytoplasma, and on nuclear mem- brane of cytotrophoblast; （2） Leptin mRNA was expressed in cytotrophoblast. Ten ng/mL leptin could promote the secretion of cytotrophoblast significantly （P〈0.01）; （3） After culture with different concentrations of leptin for 24 h or longer, the proliferation of cytotrophoblast was inhibited, while in 24 h leptin could promote cytotrophoblast invasion and migration. Leptin at a concentration of 500 ng/mL could promote cytotrophoblast invasiveness and migration significantly as compared with controls （P〈0.05）. It was suggested that leptin could inhibit cytotrophoblast proliferation, and promote cytotro- phoblast invasion and migration activity.
