Objective: Granules of herbal extracts are a popular medicinal preparation consumed in traditional Chinese medicine clinical practice. However, their quality and efficacy evaluation are lacking. This study aimed to co...Objective: Granules of herbal extracts are a popular medicinal preparation consumed in traditional Chinese medicine clinical practice. However, their quality and efficacy evaluation are lacking. This study aimed to compare the quality and anti-oxidant activity of Dan Shen(Salvia miltiorrhiza Radix & Rhizoma)granule extracts with their herbal extracts.Methods: Chromatographic method was used to determine the content of 7 marker compounds in the water extracts of the herb compared to that of 12 granule extracts. Agglomerative hierarchical clustering(AHC) and principal component analysis(PCA) distinguished the herbal and granule extracts based on the content of the marker compounds. The antioxidant activities of herbal and granule extracts were evaluated by 2, 2'-azino-bis(3-ethylbenz-thiazoline-6-sulfonic acid)(ABTS), organic chemical compound 2,2-diphenyl-1-picrylhydrazyl(DPPH) and ferric ion reducing antioxidant power(FRAP) assays.Results: The herbal extracts group showed significantly higher contents of salvianolic acid B, sodium danshensu and cryptotanshinone compared with that of the granule group. This corresponded to significantly higher ABTS, DPPH and FRAP(P <.05) activities of the herbal extracts. The AHC and PCA analysis distinguished granule extracts from most herbal extracts predominantly by the content of salvianolic acid B.Conclusion: The results confirm the need for the assessment of granule products so that healthcare practitioners and consumers are better informed of their quality and efficacy.展开更多
目的探讨复方丹参注射液对氧化所致损伤的血管内皮细胞的保护作用。方法采用脐静脉血管内皮细胞(hum anumb ilical ve in cells),用倒置相差显微镜和扫描电镜观察各组内皮细胞(endothelial cell,EC)的形态学特点。分光光度计测定细胞上...目的探讨复方丹参注射液对氧化所致损伤的血管内皮细胞的保护作用。方法采用脐静脉血管内皮细胞(hum anumb ilical ve in cells),用倒置相差显微镜和扫描电镜观察各组内皮细胞(endothelial cell,EC)的形态学特点。分光光度计测定细胞上清液中丙二醛的含量。结果扫描电镜观察显示过氧化氢组EC皱缩变小,细胞表面干裂,表面微绒毛减少甚至消失,细胞间隙增宽,而经丹参预处理过的过氧化氢组细胞形态结构则明显改善。MDA含量测定表明过氧化氢组的MDA含量明显增高。相反,正常组和丹参组中MDA含量减少(P<0.01)。结论复方丹参注射液通过其抗氧化作用,对氧化所引起的受损血管EC有保护作用。展开更多
基金supported by the Joint Chair in Traditional Chinese Medicine (JCTCM) Programfunded by the Office of Science and Research in NSW+5 种基金the University of SydneyWestern Sydney University, Australiasupported by the International Postgraduate Research Scholarship, Western Sydney Universitythe Linkage Project from the Australian Research Council (ARC) grant (LP160101594)supported by Research Support Program Fellowship, Western Sydney Universitysupported by Western Sydney University Research Grant Scheme (P00021202)。
文摘Objective: Granules of herbal extracts are a popular medicinal preparation consumed in traditional Chinese medicine clinical practice. However, their quality and efficacy evaluation are lacking. This study aimed to compare the quality and anti-oxidant activity of Dan Shen(Salvia miltiorrhiza Radix & Rhizoma)granule extracts with their herbal extracts.Methods: Chromatographic method was used to determine the content of 7 marker compounds in the water extracts of the herb compared to that of 12 granule extracts. Agglomerative hierarchical clustering(AHC) and principal component analysis(PCA) distinguished the herbal and granule extracts based on the content of the marker compounds. The antioxidant activities of herbal and granule extracts were evaluated by 2, 2'-azino-bis(3-ethylbenz-thiazoline-6-sulfonic acid)(ABTS), organic chemical compound 2,2-diphenyl-1-picrylhydrazyl(DPPH) and ferric ion reducing antioxidant power(FRAP) assays.Results: The herbal extracts group showed significantly higher contents of salvianolic acid B, sodium danshensu and cryptotanshinone compared with that of the granule group. This corresponded to significantly higher ABTS, DPPH and FRAP(P <.05) activities of the herbal extracts. The AHC and PCA analysis distinguished granule extracts from most herbal extracts predominantly by the content of salvianolic acid B.Conclusion: The results confirm the need for the assessment of granule products so that healthcare practitioners and consumers are better informed of their quality and efficacy.
文摘目的探讨复方丹参注射液对氧化所致损伤的血管内皮细胞的保护作用。方法采用脐静脉血管内皮细胞(hum anumb ilical ve in cells),用倒置相差显微镜和扫描电镜观察各组内皮细胞(endothelial cell,EC)的形态学特点。分光光度计测定细胞上清液中丙二醛的含量。结果扫描电镜观察显示过氧化氢组EC皱缩变小,细胞表面干裂,表面微绒毛减少甚至消失,细胞间隙增宽,而经丹参预处理过的过氧化氢组细胞形态结构则明显改善。MDA含量测定表明过氧化氢组的MDA含量明显增高。相反,正常组和丹参组中MDA含量减少(P<0.01)。结论复方丹参注射液通过其抗氧化作用,对氧化所引起的受损血管EC有保护作用。
