本文采用水热合成法合成表面具有大量绒毛分级结构蒲公英状Ag_3PO_4,并利用X射线衍射仪(XRD)、电子扫描显微镜(SEM)、紫外可见分光光度计等对其进行表征。实验结果表明,水热合成法制备的Ag_3PO_4与非水热合成的Ag_3PO_4颗粒在形貌上有...本文采用水热合成法合成表面具有大量绒毛分级结构蒲公英状Ag_3PO_4,并利用X射线衍射仪(XRD)、电子扫描显微镜(SEM)、紫外可见分光光度计等对其进行表征。实验结果表明,水热合成法制备的Ag_3PO_4与非水热合成的Ag_3PO_4颗粒在形貌上有明显不同。可见光下,在甲基橙(MO)光催化实验中,非水热合成法得到的Ag_3PO_4光催化效率明显低于蒲公英状Ag_3PO_4,这得益于蒲公英状Ag_3PO_4表面具有大量的光催化活性位点。蒲公英状Ag_3PO_410 min内对100 m L10 mg/L的甲基橙的降解效率是普通Ag_3PO_4颗粒的3.08倍。展开更多
Background： In the current society, infertility related to age has become a social problem. The in vitro fertilization （IVF） success rate in women with poor ovarian response （POR） is very low. Dandelion extract T...Background： In the current society, infertility related to age has become a social problem. The in vitro fertilization （IVF） success rate in women with poor ovarian response （POR） is very low. Dandelion extract T-1 （DE-T1） is an effective component of the extract from the leaves and stems of Traxacum officinale, which is one of the medicines used in some patients with POR, but its molecular mechanism remains unclear. Methods： Following IVF, ovarian granulosa cells （GCs） of sixty patients were extracted and divided into normal ovarian response （NOR） and POR groups. GCs were cultured in a dose-dependent and time-dependent manner with DE-TI, proliferation of GCs was determined by Cell Counting Kit-8 assay, and mRNA levels of insulin-like growth factor 1 receptor （IGF-1R）, luteotropic hormone receptor （LHR）, follicle-stimulating hormone receptor （FSHR）, LHR, and CYP19A1 （aromatase） were determined by quantitative polymerase chain reaction. Progesterone and estradiol （E2） concentrations were determined by enzyme-linked immunosorbent assay. Results： The cell viability gradually increased with the progressive increase in the DE-T1 concentration. Compared with the control group （without DE-T1）, the mRNA expressions of FSHR, LHR, IGF-IR, and CYPIgAI were upregulated after the addition of DE-T l, especially in the 2.5% DE-T 1 group （P 〈 0.01 ）. The expression of IGF- 1R was upregulated approximately 25 times （24.97 ± 4.02 times） in the POR group with 2.5% DE-T1. E2 and progesterone levels increased with the increasing DE-T1 concentration. There were highly significant differences in the E2 and progesterone secretion between the NOR and POR groups （P 〈 0.01）. Conclusion： DE-TI may promote steroid hormone synthesis by promoting GC proliferation and upregulating GC receptor expression, thereby improving ovarian endocrine function.展开更多
文摘本文采用水热合成法合成表面具有大量绒毛分级结构蒲公英状Ag_3PO_4,并利用X射线衍射仪(XRD)、电子扫描显微镜(SEM)、紫外可见分光光度计等对其进行表征。实验结果表明,水热合成法制备的Ag_3PO_4与非水热合成的Ag_3PO_4颗粒在形貌上有明显不同。可见光下,在甲基橙(MO)光催化实验中,非水热合成法得到的Ag_3PO_4光催化效率明显低于蒲公英状Ag_3PO_4,这得益于蒲公英状Ag_3PO_4表面具有大量的光催化活性位点。蒲公英状Ag_3PO_410 min内对100 m L10 mg/L的甲基橙的降解效率是普通Ag_3PO_4颗粒的3.08倍。
文摘Background： In the current society, infertility related to age has become a social problem. The in vitro fertilization （IVF） success rate in women with poor ovarian response （POR） is very low. Dandelion extract T-1 （DE-T1） is an effective component of the extract from the leaves and stems of Traxacum officinale, which is one of the medicines used in some patients with POR, but its molecular mechanism remains unclear. Methods： Following IVF, ovarian granulosa cells （GCs） of sixty patients were extracted and divided into normal ovarian response （NOR） and POR groups. GCs were cultured in a dose-dependent and time-dependent manner with DE-TI, proliferation of GCs was determined by Cell Counting Kit-8 assay, and mRNA levels of insulin-like growth factor 1 receptor （IGF-1R）, luteotropic hormone receptor （LHR）, follicle-stimulating hormone receptor （FSHR）, LHR, and CYP19A1 （aromatase） were determined by quantitative polymerase chain reaction. Progesterone and estradiol （E2） concentrations were determined by enzyme-linked immunosorbent assay. Results： The cell viability gradually increased with the progressive increase in the DE-T1 concentration. Compared with the control group （without DE-T1）, the mRNA expressions of FSHR, LHR, IGF-IR, and CYPIgAI were upregulated after the addition of DE-T l, especially in the 2.5% DE-T 1 group （P 〈 0.01 ）. The expression of IGF- 1R was upregulated approximately 25 times （24.97 ± 4.02 times） in the POR group with 2.5% DE-T1. E2 and progesterone levels increased with the increasing DE-T1 concentration. There were highly significant differences in the E2 and progesterone secretion between the NOR and POR groups （P 〈 0.01）. Conclusion： DE-TI may promote steroid hormone synthesis by promoting GC proliferation and upregulating GC receptor expression, thereby improving ovarian endocrine function.
