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Non-volatile dynamically switchable color display via chalcogenide stepwise cavity resonators 被引量:1
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作者 Kuan Liu Zhenyuan Lin +2 位作者 Bing Han Minghui Hong Tun Cao 《Opto-Electronic Advances》 SCIE EI CAS CSCD 2024年第1期16-27,共12页
High-resolution multi-color printing relies upon pixelated optical nanostructures,which is crucial to promote color display by producing nonbleaching colors,yet requires simplicity in fabrication and dynamic switching... High-resolution multi-color printing relies upon pixelated optical nanostructures,which is crucial to promote color display by producing nonbleaching colors,yet requires simplicity in fabrication and dynamic switching.Antimony trisulfide(Sb_(2)S_(3))is a newly rising chalcogenide material that possesses prompt and significant transition of its optical characteristics in the visible region between amorphous and crystalline phases,which holds the key to color-varying devices.Herein,we proposed a dynamically switchable color printing method using Sb_(2)S_(3)-based stepwise pixelated Fabry-Pérot(FP)cavities with various cavity lengths.The device was fabricated by employing a direct laser patterning that is a less timeconsuming,more approachable,and low-cost technique.As switching the state of Sb_(2)S_(3) between amorphous and crystalline,the multi-color of stepwise pixelated FP cavities can be actively changed.The color variation is due to the profound change in the refractive index of Sb_(2)S_(3) over the visible spectrum during its phase transition.Moreover,we directly fabricated sub-50 nm nano-grating on ultrathin Sb_(2)S_(3) laminate via microsphere 800-nm femtosecond laser irradiation in far field.The minimum feature size can be further decreased down to~45 nm(λ/17)by varying the thickness of Sb_(2)S_(3) film.Ultrafast switchable Sb_(2)S_(3) photonic devices can take one step toward the next generation of inkless erasable papers or displays and enable information encryption,camouflaging surfaces,anticounterfeiting,etc.Importantly,our work explores the prospects of rapid and rewritable fabrication of periodic structures with nano-scale resolution and can serve as a guideline for further development of chalcogenide-based photonics components. 展开更多
关键词 TUNABLE color displays Fabry-Pérot cavity resonators color printing chalcogenide materials
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Coupling of reduced inorganic fertilizer with plant-based organic fertilizer as a promising fertilizer management strategy for colored rice in tropical regions 被引量:1
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作者 Tingcheng Zhao Aibin He +3 位作者 Mohammad Nauman Khan Qi Yin Shaokun Song Lixiao Nie 《Journal of Integrative Agriculture》 SCIE CSCD 2024年第1期93-107,共15页
Colored rice is a type of high-quality,high-added-value rice that has attracted increasing attention in recent years.The use of large amounts of inorganic nitrogen fertilizer in rice fields results in low fertilizer u... Colored rice is a type of high-quality,high-added-value rice that has attracted increasing attention in recent years.The use of large amounts of inorganic nitrogen fertilizer in rice fields results in low fertilizer use efficiency and high environmental pollution.Organic fertilizer is a promising way to improve soil quality and sustain high yields.However,most studies focus on the effect of animal-based organic fertilizers.The effects of different ratios of plantbased organic fertilizer and inorganic fertilizer on the grain yield and quality of colored rice have rarely been reported.Therefore,a two-year field experiment was conducted in 2020 and 2021 to study the effects of replacing inorganic N fertilizers with plant-based organic fertilizers on the yield,nitrogen use efficiency(NUE),and anthocyanin content of two colored rice varieties in a tropical region in China.The experimental treatments included no nitrogen fertilization(T1),100% inorganic nitrogen fertilizer(T2),30%inorganic nitrogen fertilizer substitution with plant-based organic fertilizer(T3),60%inorganic nitrogen fertilizer substitution with plant-based organic fertilizer(T4),and 100% plantbased organic fertilizer(T5).The total nitrogen provided to all the treatments except T1 was the same at 120 kg ha-1.Our results showed that the T3 treatment enhanced the grain yield and anthocyanin content of colored rice by increasing nitrogen use efficiency compared with T2.On average,grain yields were increased by 9 and 8%,while the anthocyanin content increased by 16 and 10% in the two colored rice varieties under T3 across the two years,respectively,as compared with T2.Further study of the residual effect of partial substitution of inorganic fertilizers showed that the substitution of inorganic fertilizer with plant-based organic fertilizer improved the soil physiochemical properties,and thus increased the rice grain yield,in the subsequent seasons.The highest grain yield of the subsequent rice crop was observed under the T5 treatment.Our results suggested that the application of plantbased organic fertilizers can sustain the production of colored rice with high anthocyanin content in tropical regions,which is beneficial in reconciling the relationship between rice production and environmental protection. 展开更多
关键词 colored rice organic fertilizer soil quality grain yield ANTHOCYANIN
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A chromosome-level genome assembly for Chinese plum‘Wushancuili'reveals the molecular basis of its fruit color and susceptibility to rain-cracking 被引量:1
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作者 Kun Zhou Jingwen Wang +8 位作者 Lin Pan Fang Xiang Yi Zhou Wei Xiong Ming Zeng Donald Grierson Wenbin Kong Lingyu Hu Wanpeng Xi 《Horticultural Plant Journal》 SCIE CAS CSCD 2024年第3期672-688,共17页
Chinese plum(Prunus salicina Lindl.)originates from China and makes a large contribution to the global production of plums.The P.salicina‘Wushancuili'has a green coloration and high fruit quality and is economica... Chinese plum(Prunus salicina Lindl.)originates from China and makes a large contribution to the global production of plums.The P.salicina‘Wushancuili'has a green coloration and high fruit quality and is economically important in eliminating poverty and protecting ecology in the Yangtze River Three Gorges Reservoir.However,rain-induced cracking(rain-cracking,literally skin cracking caused by rain)is a limitation to‘Wushancuili'fruit production and causes severe losses.This study reported a high-quality‘Wushancuili'genome assembly consisting of a 302.17-Mb sequence with eight pseudo-chromosomes and a contig N50 of 23.59 Mb through the combination of Illumina sequencing,Pacific Biosciences HiFiⅢsequencing,and high-throughput chromosome conformation capture technology.A total of 25109 protein-coding genes are predicted and 54.17%of the genome is composed of repetitive sequences.‘Wushancuili'underwent a remarkable orthoselection during evolution.Gene identification revealed that loss-of-function in four core MYB10 genes results in the anthocyanin deficiency and absence of red color,revealing the green coloration due to the residual high chlorophyll in fruit skin.Besides,the occurrence of cracking is assumed to be closely associated with cell wall modification and frequently rain-induced pathogen enrichment through transcriptomic analysis.The loss of MYB10 genes might render fruit more susceptible to pathogen-mediated cracking by weakening the epidermal strength and reactive oxygen species(ROS)scavenging.Our findings provided fundamental knowledge regarding fruit coloration and rain-cracking and will facilitate genetic improvement and cultivation management in Chinese plums. 展开更多
关键词 Chinese plum Fruit coloration Fruit epidermis Genome MYB10 Rain-cracking
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Synthesis and Coloring Mechanism of Metallic Glaze in R_(2)O-RO-Al_(2)O_(3)-SiO_(2)-P_(2)O_(5)System
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作者 CHEN Yanlin FU Qiong +3 位作者 YANG Yuhang WEI Feng LIU Dongyang WANG Juan 《Journal of Wuhan University of Technology(Materials Science)》 SCIE EI CAS CSCD 2024年第6期1416-1424,共9页
A copper-red and silver-white metallic glaze of R_(2)O-RO-Al_(2)O_(3)-SiO_(2)-P_(2)O_(5)system was synthesized by adjusting the firing temperature and glaze components.The coloration mechanism of the metallic glaze wa... A copper-red and silver-white metallic glaze of R_(2)O-RO-Al_(2)O_(3)-SiO_(2)-P_(2)O_(5)system was synthesized by adjusting the firing temperature and glaze components.The coloration mechanism of the metallic glaze was revealed via investigation of the microstructure of the glaze.Our research reveals that the metallic glaze with different colors is mainly due to the amount of Fe_(2)O_(3).The metallic glaze shows a silver-white luster due to a structural color ofα-Fe_(2)O_(3)crystals with a good orientation when the sample contains 0.0939 mol of Fe_(2)O_(3),maintaining temperatures at 1150℃for 0.5 h.The metallic glaze is copper-red which is dominated by the coupling of chemical and structural color ofα-Fe_(2)O_(3)crystals when the sample contains 0.0783 mol of Fe_(2)O_(3).After testing the amount of SiO_(2),we find that 4.0499 mol is the optimal amount to form the ceramic network,and 0.27 mol AlPO_(4)is the best amount to promote phase separation. 展开更多
关键词 metallic glaze phase separation coloring mechanism chemical color structural color
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Identification of Shell Color-Associated Genes Using Mantle Branch-Specific RNA Sequencing of Yellow-Colored Line of Pearl Oyster Pinctada fucata martensii
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作者 PENG Haiming LIAO Yongshan +3 位作者 YANG Chuangye MKUYE Robert DENG Yuewen YUE Chenyang 《Journal of Ocean University of China》 SCIE CAS CSCD 2024年第4期1076-1086,共11页
The yellow-colored line of pearl oyster Pinctada fucata martensii displays a yellow prismatic layer and a white nacreous layer that can be used as an ideal model for research on shell color formation.Micro-Raman spect... The yellow-colored line of pearl oyster Pinctada fucata martensii displays a yellow prismatic layer and a white nacreous layer that can be used as an ideal model for research on shell color formation.Micro-Raman spectroscopy and transcriptome analyses were performed to explore the potential molecular mechanism underlying the phenotype differentiation.The micro-Raman spectroscopy results indicate that the prismatic layer exhibits distinct characteristic peaks of carotenoids,while these peaks are not prominent in the nacreous layer.In the transcriptome comparison of the central zone of mantle and mantle edge tissue,which function in nacreous and prismatic layer formation,respectively,935 significantly differentially expressed genes(DEGs)were identified,with 385 genes upregulated and 227 genes downregulated(|log_(2)(Fold change)|>1 and false discovery rate<0.05)in the mantle edge tissue.Among these genes,some were associated with melanoma/melanogenesis,such as tyrosinase,zinc metalloprotease,glutathione S-transferase,and ATP-binding cassette sub-family;some were associated with the carotenoid-related pathway,including scavenger receptors,cytochrome P450 and lipoprotein receptor.Genes associated with porphyrin metabolism,including porphobilinogen deaminase,and copper/zinc superoxide dismutase,and genes associated with shell matrix protein,including amorphous calcium carbonate binding protein,shematrin,PIF,and collagen,also exhibited significantly different expressions.It is speculated that the different colours between prismatic layer and nacreous layer in the yellow-colored line of P.f.martensii might be resulted from melanin,carotenoids and porphyrin metabolism,while genes related to shell structure and biomineralization might also affect coloration.Our results provide new insights to understand the mechanism of shell color formation in mollusca. 展开更多
关键词 Pinctada fucata martensii shell coloration TRANSCRIPTOME
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Reconfigurable and polarization-dependent optical filtering for transflective full-color generation utilizing low-loss phase-change materials
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作者 Shuo Deng Mengxi Cui +7 位作者 Jingru Jiang Chuang Wang Zengguang Cheng Huajun Sun Ming Xu Hao Tong Qiang He Xiangshui Miao 《Journal of Semiconductors》 EI CAS CSCD 2024年第7期46-53,共8页
All-dielectric metasurface, which features low optical absorptance and high resolution, is becoming a promising candidate for full-color generation. However, the optical response of current metamaterials is fixed and ... All-dielectric metasurface, which features low optical absorptance and high resolution, is becoming a promising candidate for full-color generation. However, the optical response of current metamaterials is fixed and lacks active tuning. In this work, we demonstrate a reconfigurable and polarization-dependent active color generation technique by incorporating low-loss phase change materials(PCMs) and CaF_2 all-dielectric substrate. Based on the strong Mie resonance effect and low optical absorption structure, a transflective, full-color with high color purity and gamut value is achieved. The spectrum can be dynamically manipulated by changing either the polarization of incident light or the PCM state. High transmittance and reflectance can be simultaneously achieved by using low-loss PCMs and substrate. The novel active metasurfaces can bring new inspiration in the areas of optical encryption, anti-counterfeiting, and display technologies. 展开更多
关键词 structural color RECONFIGURABLE all-dielectric metasurfaces phase change material
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MXenes for Bioinspired Soft Actuators:Advancements in Angle-Independent Structural Colors and Beyond
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作者 Siavash Iravani Rajender S.Varma 《Nano-Micro Letters》 SCIE EI CAS CSCD 2024年第8期18-34,共17页
Soft actuators have garnered substantial attention in current years in view of their potential appliances in diverse domains like robotics,biomedical devices,and biomimetic systems.These actuators mimic the natural mo... Soft actuators have garnered substantial attention in current years in view of their potential appliances in diverse domains like robotics,biomedical devices,and biomimetic systems.These actuators mimic the natural movements of living organisms,aiming to attain enhanced flexibility,adaptability,and versatility.On the other hand,angle-independent structural color has been achieved through innovative design strategies and engineering approaches.By carefully controlling the size,shape,and arrangement of nanostructures,researchers have been able to create materials exhibiting consistent colors regardless of the viewing angle.One promising class of materials that holds great potential for bioinspired soft actuators is MXenes in view of their exceptional mechanical,electrical,and optical properties.The integration of MXenes for bioinspired soft actuators with angle-independent structural color offers exciting possibilities.Overcoming material compatibility issues,improving color reproducibility,scalability,durability,power supply efficiency,and cost-effectiveness will play vital roles in advancing these technologies.This perspective appraises the development of bioinspired MXene-centered soft actuators with angleindependent structural color in soft robotics. 展开更多
关键词 MXenes MXene-based composites Bioinspired soft robotics Angle-independent structural color
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Quantitative trait loci identification reveals zinc finger protein CONSTANS-LIKE 4 as the key candidate gene of stigma color in watermelon(Citrullus lanatus)
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作者 Shuang Pei Zexu Wu +4 位作者 Ziqiao Ji Zheng Liu Zicheng Zhu Feishi Luan Shi Liu 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2024年第7期2292-2305,共14页
Stigma color is a critical agronomic trait in watermelon that plays an important role in pollination.However,there are few reports on the regulation of stigma color in watermelon.In this study,a genetic analysis of th... Stigma color is a critical agronomic trait in watermelon that plays an important role in pollination.However,there are few reports on the regulation of stigma color in watermelon.In this study,a genetic analysis of the F2 population derived from ZXG1553(P1,with orange stigma)and W1-17(P2,with yellow stigma)indicated that stigma color is a quantitative trait and the orange stigma is recessive compared with the yellow stigma.Bulk segregant analysis sequencing(BSA-seq)revealed a 3.75 Mb segment on chromosome 6 that is related to stigma color.Also,a major stable effective QTL Clqsc6.1(QTL stigma color)was detected in two years between cleaved amplified polymorphic sequencing(CAPS)markers Chr06_8338913 and Chr06_9344593 spanning a~1.01 Mb interval that harbors 51 annotated genes.Cla97C06G117020(annotated as zinc finger protein CONSTANS-LIKE 4)was identified as the best candidate gene for the stigma color trait through RNA-seq,quantitative real-time PCR(qRT-PCR),and gene structure alignment analysis among the natural watermelon panel.The expression level of Cla97C06G117020 in the orange stigma accession was lower than in the yellow stigma accessions with a significant difference.A nonsynonymous SNP site of the Cla97C06G117020 coding region that causes amino acid variation was related to the stigma color variation among nine watermelon accessions according to their re-sequencing data.Stigma color formation is often related to carotenoids,and we also found that the expression trend of ClCHYB(annotated asβ-carotene hydroxylase)in the carotenoid metabolic pathway was consistent with Cla97C06G117020,and it was expressed in low amounts in the orange stigma accession.These data indicated that Cla97C06G117020 and ClCHYB may interact to form the stigma color.This study provides a theoretical basis for gene fine mapping and mechanisms for the regulation of stigma color in watermelon. 展开更多
关键词 WATERMELON stigma color gene mapping zinc finger protein CONSTANS-LIKE 4
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Fine-mapping and primary analysis of candidate genes associated with seed coat color in mung bean(Vigna radiata L.)
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作者 Qian Wang Huimin Cao +10 位作者 Jingcheng Wang Zirong Gu Qiuyun Lin Zeyan Zhang Xueying Zhao Wei Gao Huijun Zhu Hubin Yan Jianjun Yan Qingting Hao Yaowen Zhang 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2024年第8期2571-2588,共18页
Seed coat color affects the appearance and commodity quality of mung beans(Vigna radiata L.).The substances that affect mung bean seed coat color are mainly flavonoids,which have important medicinal value.Mapping the ... Seed coat color affects the appearance and commodity quality of mung beans(Vigna radiata L.).The substances that affect mung bean seed coat color are mainly flavonoids,which have important medicinal value.Mapping the seed coat color gene in mung beans would facilitate the development of new varieties and improve their value.In this study,an F2 mapping population consisting of 546 plants was constructed using Jilv9(black seed coat)and BIS9805(green seed coat).Using bulk segregated analysis(BSA)sequencing and kompetitive allele-specific PCR(KASP)markers,the candidate region related to seed coat color was finally narrowed to 0.66 Mb on chromosome(Chr.)4 and included eight candidate genes.Combined transcriptome and metabolome analyses showed that three of the eight candidate genes(LOC106758748,LOC106758747,and LOC106759075)were differentially expressed,which may have caused the differences in flavonoid metabolite content between Jilv9 and BIS9805.These findings can provide a research basis for cloning the genes related to seed coat color and accelerate molecular markerassisted selection breeding in mung beans. 展开更多
关键词 mung bean seed coat color bulk segregated analysis sequencing TRANSCRIPTOME METABOLISM FLAVONOIDS
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CmMYB3-like negatively regulates anthocyanin biosynthesis and flower color formation during the post-flowering stage in Chrysanthemum morifolium
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作者 Lijie Zhou Shenhui Liu +6 位作者 Yiguang Wang Yuxi Wang Aiping Song Jiafu Jiang Sumei Chen Zhiyong Guan Fadi Chen 《Horticultural Plant Journal》 SCIE CAS CSCD 2024年第1期194-204,共11页
Color fading caused by a decrease in anthocyanin accumulation during the post-flowering stage significantly affects postharvest quality of chrysanthemum.However,the underlying mechanism by which anthocyanin accumulati... Color fading caused by a decrease in anthocyanin accumulation during the post-flowering stage significantly affects postharvest quality of chrysanthemum.However,the underlying mechanism by which anthocyanin accumulation decreases during the post-flowering stage still unclear,which greatly restricts design of molecular breeding in chrysanthemum.Here,a chrysanthemum SG7 R2R3 MYB transcription factor(TF),CmMYB3-like,was identified to have a function in regulating anthocyanin biosynthesis during the post-flowering stage.Quantitative real time PCR(qRT-PCR)assays showed that the expression of CmMYB3-like was gradually downregulated when anthocyanin content increased during the flowering stage and was significantly upregulated during the post-flowering stage.Genetic transformation of chrysanthemum and dual-luciferase assays in N.benthamiana leaves showed that CmMYB3-like suppressed anthocyanin accumulation by inhibiting the transcription of CmCHS and CmANS directly and that of CmF3H indirectly.However,overexpression or suppression of CmMYB3-like did not affect the biosynthesis of flavones or flavonols.Genetic transformation of chrysanthemum revealed that the overexpression of CmMYB3-like inhibited anthocyanin accumulation,but its suppression prevented the decrease in anthocyanin accumulation during the post-flowering stage.Our results revealed a crucial role of CmMYB3-like in regulating the color of petals during the post-flowering stage and provided a target gene for molecular design breeding to improve the postharvest quality of chrysanthemum. 展开更多
关键词 Post-flowering stage color fading Anthocyanins CmMYB3-like CHRYSANTHEMUM Molecular breeding
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Low-fat microwaved peanut snacks production:Effect of defatting treatment on structural characteristics,texture,color,and nutrition
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作者 Bo Jiao Xin Guo +6 位作者 Yiying Chen Shah Faisal Wenchao Liu Xiaojie Ma Bicong Wu Guangyue Ren Qiang Wang 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2024年第7期2491-2502,共12页
This study develops low-fat microwaved peanut snacks(LMPS)using partially defatted peanuts(PDP)with different defatting ratios,catering to people’s pursuit of healthy,low-fat cuisine.The effects of defatting treatmen... This study develops low-fat microwaved peanut snacks(LMPS)using partially defatted peanuts(PDP)with different defatting ratios,catering to people’s pursuit of healthy,low-fat cuisine.The effects of defatting treatment on the structural characteristics,texture,color,and nutrient composition of LMPS were comprehensively explored.The structural characteristics of LMPS were characterized using X-ray micro-computed tomography(Micro-CT)and scanning electron microscope(SEM).The results demonstrated that the porosity,pore number,pore volume,brightness,brittleness,protein content,and total sugar content of LMPS all significantly increased(P<0.05)with the increase in the defatting ratio.At the micro level,porous structure,cell wall rupture,and loss of intracellular material could be observed in LMPS after defatting treatments.LMPS made from PDP with a defatting ratio of 64.44%had the highest internal pore structural parameters(porosity 59%,pore number 85.3×10^(5),pore volume 68.23 mm3),the brightest color(L^(*) 78.39±0.39),the best brittleness(3.64±0.21)mm^(–1)),and the best nutrition(high protein content,(34.02±0.38)%;high total sugar content,(17.45±0.59)%;low-fat content,(27.58±0.85)%).The study provides a theoretical basis for the quality improvement of LMPS. 展开更多
关键词 low-fat microwaved peanut snacks(LMPS) X-ray micro-computed tomography(Micro-CT) porosity TEXTURE color NUTRITION
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A Cognitive Psychological Study on the Acquisition of Chinese Word’s Coloring Meaning--Chinese as a Second Language
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作者 LIU Yan 《Sino-US English Teaching》 2024年第5期217-221,共5页
Lexical meaning mainly includes rational meaning,grammatical meaning,and coloring meaning.Mastering the coloring meaning of vocabulary is of great significance for foreign students to use Chinese vocabulary correctly.... Lexical meaning mainly includes rational meaning,grammatical meaning,and coloring meaning.Mastering the coloring meaning of vocabulary is of great significance for foreign students to use Chinese vocabulary correctly.This study mainly examines the psychological mechanism of Chinese second language learners mastering the coloring meaning of words,examines the psychological characteristics of students mastering the color meaning of words from the perspectives of second language learning theory and cognitive theory,establishes a cognitive schema for coloring meaning learning,and proposes corresponding learning models and teaching strategies. 展开更多
关键词 Chinese vocabulary acquisition coloring meaning COGNITION
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Application of Color Psychology in Korean Brand Identity Design-Etude House
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作者 Jingyao Luo Choi Albort Young 《Cultural and Religious Studies》 2024年第1期24-31,共8页
The purpose of this analysis is to delve into the application of color psychology in the logo design of Korean cosmetic brands,using Etude House as an example for an exhaustive analysis.By examining the history of the... The purpose of this analysis is to delve into the application of color psychology in the logo design of Korean cosmetic brands,using Etude House as an example for an exhaustive analysis.By examining the history of the Etude House brand,the evolution of the logo design,and the changes in color choices,we analyze the traditional concepts of color symbolism in Korean culture and the culture of color in contemporary society in order to reveal the important role of color in cosmetic brand image.Through an in-depth analysis of the use of color in Etude House’s brand identity,we further analyze the impact of color on consumer emotions and purchasing behavior,as well as the potential impact of brand identity changes on market performance.Finally,the conclusions of the analysis summarize the practical application of color psychology in Etude House’s brand logo design,suggest recommendations for other Korean cosmetic brands to draw upon in their logo design,and discuss future directions. 展开更多
关键词 color psychology brand identity design Etude House Korean cosmetics consumer emotions market performance
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Exosome-Transmitted miR-224-5p Promotes Colorectal Cancer Cell Proliferation via Targeting ULK2 in p53-Dependent Manner
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作者 YANG Le Mei ZHENG Qi +5 位作者 LIU Xiao Jia LI Xian Xian Veronica Lim CHEN Qi ZHAO Zhong Hua WANG Shu Yang 《Biomedical and Environmental Sciences》 SCIE CAS CSCD 2024年第1期71-84,共14页
Objective To investigate the role and molecular mechanism of exosomal miR-224-5p in colorectal cancer(CRC).Methods The miR-224-5p expression in CRC patient tissues and cell-derived exosomes was measured by laser captu... Objective To investigate the role and molecular mechanism of exosomal miR-224-5p in colorectal cancer(CRC).Methods The miR-224-5p expression in CRC patient tissues and cell-derived exosomes was measured by laser capture microdissection and qRT-PCR,respectively.Dual-luciferase reporter gene assay was used to determine the target gene of miR-224-5p.The protein expressions of p53 and unc-51 like kinase 2(ULK2)in CRC cells were detected by western blot.Flow cytometry was used to detect cell cycle and apoptosis.Cell proliferation was measured by CCK8 and EdU assay.Results The miR-224-5p expression was upregulated in CRC tissues and increased progressively with the rise of CRC stage.CRC cells secreted extracellular miR-224-5p mainly in an exosome-dependent manner,and then miR-224-5p could be transferred to surrounding tumor cells to regulate cell proliferation in the form of autocrine or paracrine.Moreover,ULK2 was characterized as a direct target of miR-224-5p and was downregulated in CRC tissues.Interestingly,ULK2 inhibited CRC cell proliferation in a p53-dependent manner.Furthermore,exosome-derived miR-224-5p partially reversed the proliferation regulation of ULK2 on CRC cells.Conclusion Our findings demonstrate that exosome-transmitted miR-224-5p promotes p53-dependent cell proliferation by targeting ULK2 in CRC,which may offer promising targets for CRC prevention and therapy. 展开更多
关键词 miR-224-5p EXOSOME ULK2 P53 Cell proliferation colorectal cancer
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Association of KRAS Gene and microRNA-124-3p in Sporadic Colorectal Tumours
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作者 Ozkan Bagci 《Journal of Biosciences and Medicines》 2024年第1期150-161,共12页
Aim: To reveal the exonic and 3’UTR sequences of KRAS, TP53, APC, BRAF, PIK3CA genes in sporadic colorectal tumors and to investigate the clinical relevance of 3’UTR variations in miRNA profiles. Methods: In the stu... Aim: To reveal the exonic and 3’UTR sequences of KRAS, TP53, APC, BRAF, PIK3CA genes in sporadic colorectal tumors and to investigate the clinical relevance of 3’UTR variations in miRNA profiles. Methods: In the study, the exonic and 3’UTR sequences of five genes in 12 sporadic colorectal tumors were extracted by next generation sequencing. In tumors with variation in the 3’UTR region, the changes caused by the variation in the miRNA binding profile were detected. The expression profile of these miRNAs in colorectal and other solid tumors compared to normal tissue was determined. Pathway analysis was performed to determine which signaling pathways miRNAs affect. Results: Case-10 in our study was wild type KRAS and received cetuximab treatment and developed drug resistance. In this case, it was concluded that the expression of KRAS increased and tumorigenesis progressed due to miRNAs that do not bind to this region due to variations in the 3’UTR region. Among these miRNAs, hsa-miR-124-3p was found to have decreased expression in colorectal tumors and to be associated with the ECM-receptor interaction pathway. Conclusion: Variations in the 3’UTR regions of genes critical in the process of carsinogenesis are associated with drug resistance and the process of tumorigenesis. 展开更多
关键词 colorectal Tumours Drug Resistance Personalised Medicine microRNA-124-3p
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On the (Δ + 2)-Total-Colorability of Planar Graphs with 7-Cycles Containing at Most Two Chords
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作者 Jian Chang Jingru Liu Fan Zhang 《Journal of Applied Mathematics and Physics》 2024年第7期2702-2710,共9页
The Total Coloring Conjecture (TCC) proposes that every simple graph G is (Δ + 2)-totally-colorable, where Δ is the maximum degree of G. For planar graph, TCC is open only in case Δ = 6. In this paper, we prove tha... The Total Coloring Conjecture (TCC) proposes that every simple graph G is (Δ + 2)-totally-colorable, where Δ is the maximum degree of G. For planar graph, TCC is open only in case Δ = 6. In this paper, we prove that TCC holds for planar graph with Δ = 6 and every 7-cycle contains at most two chords. 展开更多
关键词 Planar Graph 7-Cycle 8-Totally-colorable Maximum Degree
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The combined detection of carcinoembryonic antigen,carcinogenic antigen 125,and carcinogenic antigen 19-9 in colorectal cancer patients
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作者 Ling-Zhen Gong Qian-Wen Wang Jie-Wen Zhu 《World Journal of Gastrointestinal Surgery》 SCIE 2024年第7期2073-2079,共7页
BACKGROUND Hepatic metastases are common and difficult to treat after colorectal cancer(CRC)surgery.The predictive value of carcinoembryonic antigen(CEA),cancer antigen(CA)125 and CA19-9 combined tests for liver metas... BACKGROUND Hepatic metastases are common and difficult to treat after colorectal cancer(CRC)surgery.The predictive value of carcinoembryonic antigen(CEA),cancer antigen(CA)125 and CA19-9 combined tests for liver metastasis is unclear.AIM To evaluate predictive value of combined tests for CEA,CA125,and CA19-9 levels in patients with liver metastases of CRC.METHODS The retrospective study included patients with CRC alone(50 cases)and patients with CRC combined with liver metastases(50 cases)who were hospitalized between January 2021 and January 2023.Serum CEA,CA125 and CA19-9 levels were compared between the two groups,and binary logistic regression was used to analyze the predictive value of the combination of these tumor markers in liver metastasis.In addition,we performed receiver operating characteristic(ROC)curve analysis to assess its diagnostic accuracy.RESULTS The results showed that the serum CEA,CA125 and CA19-9 levels in the CRC with liver metastasis group were significantly higher than those in the CRC alone group.Specifically,the average serum CEA level in the CRC with liver metastasis group was 162.03±810.01 ng/mL,while that in the CRC alone group was 5.71±9.76 ng/mL;the average serum CA125 levels were 43.47±83.52 U/mL respectively.and 13.5±19.68 U/mL;the average serum CA19-9 levels were 184.46±473.13 U/mL and 26.55±43.96 U/mL respectively.In addition,binary logistic regression analysis showed that CA125 was significant in predicting CRC liver metastasis(P<0.05).ROC curve analysis results showed that the areas under the ROC curves of CEA,CA125 and CA19-9 were 0.607,0.692 and 0.586.CONCLUSION These results suggest that combined detection of these tumor markers may help early detection and intervention of CRC liver metastasis,thereby improving patient prognosis. 展开更多
关键词 colorectal cancer Liver metastasis Serum markers Carcinoembryonic antigen Cancer antigen 125 Cancer antigen 19-9 Receiver operating characteristic analysis
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基于特征图像组合与改进ResNet-18的电能质量扰动识别方法 被引量:2
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作者 张逸 欧杰宇 +1 位作者 金涛 毕贵红 《中国电机工程学报》 EI CSCD 北大核心 2024年第7期2531-2544,I0003,共15页
针对传统电能质量扰动(power quality disturbance,PQD)识别体系中单一图像特征信息受限与算法识别能力不足等问题,依据特征融合的思想,提出一种基于特征图像组合与改进ResNet-18的PQD识别方法。首先,对PQD信号进行变分模态分解(variati... 针对传统电能质量扰动(power quality disturbance,PQD)识别体系中单一图像特征信息受限与算法识别能力不足等问题,依据特征融合的思想,提出一种基于特征图像组合与改进ResNet-18的PQD识别方法。首先,对PQD信号进行变分模态分解(variational mode decomposition,VMD)得到一系列固有模态函数(intrinsic mode functions,IMFs)与残差分量;其次,将IMFs、残差分量、原始扰动信号与Subtract分量纵向拼接成分量矩阵,利用信号-图像转化方法生成特征分量彩色图;再次,对原始扰动信号进行连续小波变换(continuous wavelet transform,CWT)生成小波时-频图;最后,将特征分量彩色图与小波时-频图组合输入改进的六通道ResNet-18中训练学习并完成扰动识别。通过仿真对PQD识别方法进行分析并将其与目前常用识别体系进行比较。结果表明,所提方法具有较好的抗噪性能并且能够更好地提取PQD特征信息,达到更高的识别准确率。 展开更多
关键词 电能质量扰动 变分模态分解 特征分量彩色图 小波时-频图 残差网络
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基于SPI-RRV指数中国气象干旱及其风险时空演变特征研究 被引量:3
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作者 杨肖丽 罗定 +4 位作者 叶周兵 谢灵枫 任立良 江善虎 袁山水 《水资源保护》 EI CSCD 北大核心 2024年第1期44-51,共8页
为全面揭示变化环境下我国多维气象干旱特征,耦合气象干旱指数(SPI)和可靠性-回弹性-脆弱性(RRV)指数,提出了一种基于SPI-RRV指数的干旱风险评价方法,定量评价了中国气象干旱及其风险的时空演变特征。结果表明:SPI-RRV指数具有特征稳定... 为全面揭示变化环境下我国多维气象干旱特征,耦合气象干旱指数(SPI)和可靠性-回弹性-脆弱性(RRV)指数,提出了一种基于SPI-RRV指数的干旱风险评价方法,定量评价了中国气象干旱及其风险的时空演变特征。结果表明:SPI-RRV指数具有特征稳定和时空可比性强的特点,能够较为准确地评估气象干旱风险时空演变特征;南方平均干旱栅格比、干旱月占比和频次大于北方,湿润区和半湿润区干旱历时短、烈度大,半干旱区和干旱区干旱历时长、烈度相对较小;干旱高风险区转移具有显著年代际变化规律,空间上从西北向西南地区转移。 展开更多
关键词 气象干旱 标准化降水指数 可靠性-回弹性-脆弱性指数 干旱风险
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深部煤储层孔裂隙结构对煤层气赋存的影响-以鄂尔多斯盆地东缘大宁-吉县区块为例 被引量:2
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作者 邓泽 王红岩 +3 位作者 姜振学 丁蓉 李永洲 王涛 《煤炭科学技术》 EI CAS CSCD 北大核心 2024年第8期106-123,共18页
深部煤储层孔隙–裂缝结构对深部煤层气资源潜力评价和勘探开发具有重要意义。选取鄂尔多斯盆地东缘大宁–吉县区块DJ57井本溪组5个煤岩样品为研究对象,在煤岩煤质参数测试的基础上,采用气体吸附法、高压压汞法和微米CT扫描等测试手段,... 深部煤储层孔隙–裂缝结构对深部煤层气资源潜力评价和勘探开发具有重要意义。选取鄂尔多斯盆地东缘大宁–吉县区块DJ57井本溪组5个煤岩样品为研究对象,在煤岩煤质参数测试的基础上,采用气体吸附法、高压压汞法和微米CT扫描等测试手段,对深部煤储层中的纳米级孔隙-微米级裂缝进行多尺度定量表征,综合评价不同尺度的孔裂隙结构特征。再结合渗透率和甲烷等温吸附试验,探讨了微观孔裂隙对深部煤储层中煤层气的赋存和渗流的影响。研究结果表明:基于多种孔隙表征方法对深部煤储层孔裂隙进行多尺度定量表征,其孔裂隙体积分布类型主要以“U”型为主,呈现出微孔与微裂缝并存双峰态,主要集中在0.3~1.5 nm和>100μm的范围内。其中,微孔(<2 nm)、介孔(2~50 nm)、宏孔(50 nm~10μm)和微裂缝(>10μm)体积平均分别占总孔裂隙体积的80.18%,6.70%,1.65%和11.47%。随着微孔发育而吸附气量呈增大的趋势,微孔可以提供大量吸附点位,为深部煤层气的吸附和赋存提供场所。随着微裂缝发育而游离气量呈增大的趋势,微裂缝可以提供大量储集空间,为深部煤层气的富集提供空间条件。此外,微裂缝在三维空间中相互连通,形成网状结构,连通性强。随着微裂缝越发育,煤储层渗透率越大,微裂缝增强了煤层气的渗流能力。纳米级孔隙和微米级裂隙发育特征分别控制着深部煤层气吸附能力和开发潜力。 展开更多
关键词 深部煤层气 孔隙-裂缝 全尺度表征 大宁-吉县区块
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