The germination of pollen grain in vitro and the growth of pollen tube in the pistil of rice were observed with a microscope. The stigma was removed at different time points after pollination to study its effect on se...The germination of pollen grain in vitro and the growth of pollen tube in the pistil of rice were observed with a microscope. The stigma was removed at different time points after pollination to study its effect on seed setting rate. The rice pollen grain started to germinate at 2 min after pollination and the pollen tube penetrated stigma into style in 5-10 min, 30 min later the end of pollen tube reached the bottom of ovary, and only some pollen tubes arrived at embryo sac at 40 min after pollination. Meanwhile, a small amount of callose began to deposit in the pollen tubes, a great deal of callose was observed at 50 min after pollination, whereas the pollen grain began to shrink. The growing rates of pollen tube in the rice stigma, style and ovary were 1500, 5000, and 5400 μm/h, respectively. The seed setting rate was quite low when the stigma was removed at about 10-15 min after pollination, gradually increased when it removed at 20 min to 50 min after pollination, and over 60% when it removed at 50 min after pollination and finally tended to be stable.展开更多
The airborne pollen grains of Afyon have been studied for a two_year period (1999-2000) with a Durham sampler. A total of 14 367 pollen grains belonging to 40 taxa have been identified and recorded with some unidentif...The airborne pollen grains of Afyon have been studied for a two_year period (1999-2000) with a Durham sampler. A total of 14 367 pollen grains belonging to 40 taxa have been identified and recorded with some unidentified ones. Of them, 6 732 were identified in 1999 and 7 635 in 2000. Of the total pollen grains, 69.67% were arboreal, 26.64% non_arboreal and 3.68 % unidentified. The majority of the investigated pollen grains were from Pinus, Gramineae, Cupressaceae, Platanus , Chenopodiaceae/Amaranthaceae, Quercus, Ailanthus, Moraceae, Juglans , Salix, Cedrus and Rosaceae. The highest level of pollen grains was in May.展开更多
Pollen grains and plant spores have emerged as innovative biomaterials for various applications such as drug/vaccine delivery,catalyst support,and the removal of heavy metals.The natural microcapsules comprising spore...Pollen grains and plant spores have emerged as innovative biomaterials for various applications such as drug/vaccine delivery,catalyst support,and the removal of heavy metals.The natural microcapsules comprising spore shells and pollen grain are designed for protecting the genetic materials of plants from exterior impairments.Two layers make up the shell,the outer layer(exine)that comprised largely of sporopollenin,and the inner layer(intine)that built chiefly of cellulose.These microcapsule shells,namely hollow sporopollenin exine capsules have some salient features such as homogeneity in size,non-toxic nature,resilience to both alkalis and acids,and the potential to withstand at elevated temperatures;they have displayed promising potential for the microencapsulation and the controlled drug delivery/release.The important attribute of mucoadhesion to intestinal tissues can prolong the interaction of sporopollenin with the intestinal mucosa directing to an augmented effectiveness of nutraceutical or drug delivery.Here,current trends and prospects related to the application of plant pollen grains for the delivery of vaccines and drugs and vaccine are discussed.展开更多
Polygala paniculata L.is a medicinal plant that grows in the Brazilian Atlantic coast,known as‘barba-de-São-João’,‘barba-de-bode’,‘vassourinha branca’,and‘mimosa’.In this study,pollen viability was e...Polygala paniculata L.is a medicinal plant that grows in the Brazilian Atlantic coast,known as‘barba-de-São-João’,‘barba-de-bode’,‘vassourinha branca’,and‘mimosa’.In this study,pollen viability was estimated by three different staining methods:2%acetic orcein,2%acetic carmine,and Alexander’s stain.The young inflorescences of twenty accessions were collected and fixed in a solution of ethanol:acetic acid(3:1)for 24 hours,then stored in ethanol 70%under refrigeration.Six slides per plant,two for each stain,were prepared by squashing,and 300 pollen grains per slide were analyzed.Pollen viability was high(>70%)for most accessions of P.paniculata using the Alexander’s stain,which proved the most adequate method to estimate pollen viability.展开更多
Aim of study: Ancient literature alluded to the use of a number of plants/preparations as sex enhancer. One of such botanicals is Phoenix dactylifera in which the pollen grain has been acclaimed to be used as an aphro...Aim of study: Ancient literature alluded to the use of a number of plants/preparations as sex enhancer. One of such botanicals is Phoenix dactylifera in which the pollen grain has been acclaimed to be used as an aphrodisiac. However, the validity has not been scientifically tested. Dopamine is known to facilitate male sexual function. Therefore, the present study was undertaken to evaluate the effect of aqueous extract of Phoenix dactylifera pollen on the sexual behavior of male rats and to measure of serum Estradiol and Testostrone. Also, dopamine transmission in the nucleus accumbence (NAc) was studied in male rats using in vivo microdialysis. Methods and Materials: sixty male rats were randomized into 6 groups (A-F). Group A received 0.2 ml of Normal Saline mixed with Dimethyl Sulphate (DMSO), while groups B-F were injected same volume containing 35 mg/kg, 70 mg/kg, 105 mg/kg, 140 mg/kg and 350 mg/kg of DPP extract, respectively. Sexual behavioral parameters including mounting, intromission and ejaculation frequencies and latencies were recorded in male rats one hour after injection of extract by mating with a receptive female (1:1). The male serum testosterone and estradiol concentrations were also determined. Results: All doses stimulated male sexual behavior. Extract significantly increased mount, ejaculation, intromission frequencies and ejaculation latency in comparison to controlled ones (p 0.001). Mount and intromission latencies significantly reduced (p 0.001). Maximum effect was observed in dose 140 mg/kg. This extract was found to enhance Testestrone, Estradiol and the orientation of males toward female ones by increasing mounting and ano-genital investigatory behavior. Conclusions: Data from this study identified that the aqueous extract of Phoenix dactylifera pollen grain enhanced sexual behaviour in male rats. The improved sexual appetitive behaviour in male rats may be attributed, to the alkaloids, saponins, and or flavonoids since these phytochemicals has engorgement, androgen enhancing. Also, our findings support the traditional use of this plant as acclaimed aphrodisiac and for the treatment of pre-ejaculation and impotency.展开更多
The aim of the current study was to investigate the process of microgametogenesis and diagnostic of condition of the Pinus sylvestris male generative system in the tree stands exposed to the influence of Reftinskiy GR...The aim of the current study was to investigate the process of microgametogenesis and diagnostic of condition of the Pinus sylvestris male generative system in the tree stands exposed to the influence of Reftinskiy GRES power plant (RGpp) emission during ontogenesis in connection with the level of technogenic pollution. It was detected that in the impact area, smoke emissions from the RGpp in the south-west direction zones of technogenic pollution were formed. Those vary in qualitative composition of the snow pack pollutants and their quantitative characteristics. The content of water insoluble compounds in melt snow water and the compile suspended matter, decreases regularly at the longer distance from the source of air pollutants. The highest content of water soluble compounds in the melt snow water was indicated at the distances of 5, 8 and 14 km from the source of air pollutants, authentically lower in the nearest and farthest sampling points of the snow trials. Scots pine (Pinus sylvestris) pollen significative with high prognostic value in relation to the level of toxic load upon the eco-systems and the condition of male generative system were discovered. It was detected that the significative meanings of pollen with morphological abnormalities decreased, and the meanings of functional significatives increased at the longer distance from the source of air pollutants. Pollen fertility, pollen tube length and reserve substances content significative is negatively related to the presence of micro and macro elements in the snow water;those identify the toxic load level on the ecosystem probably to the highest degree. Pine male gametophyte elimination, while forming in the impact zone of the GRES power plant, takes place at the stage of pollen grain germination as well as pollen tubes’ growth stage.展开更多
A medicolegal/botanical collaboration has to lead to an interdisciplinary procedure for developing methods when studying dead bodies found in water with no clear circumstances. Hereby we present a report or a prelimin...A medicolegal/botanical collaboration has to lead to an interdisciplinary procedure for developing methods when studying dead bodies found in water with no clear circumstances. Hereby we present a report or a preliminary attempt as a basis for encouraging further work in the field. The body of an unidentified 25 - 35 years old woman was found immersed in a fresh water ditch in state of decomposition. Structural alterations avoid any a priori identification of asphyxia or traumatic cervical lesions. The last cause of death was estimated as a cardio respiratory collapse and a drowning investigation was proposed. Taxonomic species identification was carried out to relate the pollen aspiration with the water medium in lung, spleen, liver and water medium. We presented pollen grains in spleen, as a closed organ, as a clue for death investigation in the absence of diatom findings. Information about where and when pollen grains occur may ascertain that a body or an object was in some place at a certain time. Since the consequences of aspiration depend on many factors, such as the quantity, size and solidity of the material aspirated and whether the aspiration is chronically recurrent or acute, the pollen investigation must be done with precaution. We conclude that in the absence of other microscopic structures, biochemical blood analyses, or histological findings, forensic palynology may be of help to relate the presumptive drowned body with its death scenario.展开更多
良渚遗址群是距杭州市西北18 km的良渚镇和瓶窑镇毗邻地带间许多遗址的总称,代表了良渚文化发展的最高成就,是良渚文化的中心。根据在2007年发现的良渚古城墙外侧开挖的良渚北剖面、良渚西剖面沉积样品的粒度分析、孢粉分析数据,以及综...良渚遗址群是距杭州市西北18 km的良渚镇和瓶窑镇毗邻地带间许多遗址的总称,代表了良渚文化发展的最高成就,是良渚文化的中心。根据在2007年发现的良渚古城墙外侧开挖的良渚北剖面、良渚西剖面沉积样品的粒度分析、孢粉分析数据,以及综合区域内多个剖面地层对比,恢复了该地区全新世环境的演变过程。研究表明,全新世良渚遗址群内环境演变可以分为4个阶段：（1）7 ka BP以前的平原区多为静水沉积环境,水域范围较大,水位较高,且水深逐渐增加,不太适合人类活动;（2）7~5.1 ka BP区域水位有所下降,水域面积缩小,部分较高的地区露出水面,较少接受沉积,一些低洼地区接受了沼泽相或河流泛滥相沉积,一些人类活动遗址开始出现;（3）5.1~4.3 ka BP水位较低,文化快速发展,平原上多见良渚文化遗迹,一些遗址堆积了较厚的良渚文化层;（4）4.3 ka BP以后水位再次升高,良渚文化衰落,多数良渚文化层之上都覆盖一套黄色或黄褐色泛滥相沉积。该地区全新世时期的水位波动与人类文化兴衰演化历史相关的现象表明,良渚遗址区域内地貌特征及水文环境的变化是影响良渚文化兴衰演化的重要原因之一。展开更多
基金the National Natural Science Foundation of China (Grants Nos. 30070454 and 30471045)
文摘The germination of pollen grain in vitro and the growth of pollen tube in the pistil of rice were observed with a microscope. The stigma was removed at different time points after pollination to study its effect on seed setting rate. The rice pollen grain started to germinate at 2 min after pollination and the pollen tube penetrated stigma into style in 5-10 min, 30 min later the end of pollen tube reached the bottom of ovary, and only some pollen tubes arrived at embryo sac at 40 min after pollination. Meanwhile, a small amount of callose began to deposit in the pollen tubes, a great deal of callose was observed at 50 min after pollination, whereas the pollen grain began to shrink. The growing rates of pollen tube in the rice stigma, style and ovary were 1500, 5000, and 5400 μm/h, respectively. The seed setting rate was quite low when the stigma was removed at about 10-15 min after pollination, gradually increased when it removed at 20 min to 50 min after pollination, and over 60% when it removed at 50 min after pollination and finally tended to be stable.
文摘The airborne pollen grains of Afyon have been studied for a two_year period (1999-2000) with a Durham sampler. A total of 14 367 pollen grains belonging to 40 taxa have been identified and recorded with some unidentified ones. Of them, 6 732 were identified in 1999 and 7 635 in 2000. Of the total pollen grains, 69.67% were arboreal, 26.64% non_arboreal and 3.68 % unidentified. The majority of the investigated pollen grains were from Pinus, Gramineae, Cupressaceae, Platanus , Chenopodiaceae/Amaranthaceae, Quercus, Ailanthus, Moraceae, Juglans , Salix, Cedrus and Rosaceae. The highest level of pollen grains was in May.
文摘Pollen grains and plant spores have emerged as innovative biomaterials for various applications such as drug/vaccine delivery,catalyst support,and the removal of heavy metals.The natural microcapsules comprising spore shells and pollen grain are designed for protecting the genetic materials of plants from exterior impairments.Two layers make up the shell,the outer layer(exine)that comprised largely of sporopollenin,and the inner layer(intine)that built chiefly of cellulose.These microcapsule shells,namely hollow sporopollenin exine capsules have some salient features such as homogeneity in size,non-toxic nature,resilience to both alkalis and acids,and the potential to withstand at elevated temperatures;they have displayed promising potential for the microencapsulation and the controlled drug delivery/release.The important attribute of mucoadhesion to intestinal tissues can prolong the interaction of sporopollenin with the intestinal mucosa directing to an augmented effectiveness of nutraceutical or drug delivery.Here,current trends and prospects related to the application of plant pollen grains for the delivery of vaccines and drugs and vaccine are discussed.
基金Acknowledgments We are grateful to Dr Michael E Maguire （Case Western Reserve University, USA） for providing the Salmonella mutant strain MM281 and advice on tracer uptake. This work was supported by grants from National Natural Science Foundation of China （30370794）, Hunan Young Scientist Program （03JJY1003）, and Hunan Provincial NSFC （08JJ3088）.
文摘Polygala paniculata L.is a medicinal plant that grows in the Brazilian Atlantic coast,known as‘barba-de-São-João’,‘barba-de-bode’,‘vassourinha branca’,and‘mimosa’.In this study,pollen viability was estimated by three different staining methods:2%acetic orcein,2%acetic carmine,and Alexander’s stain.The young inflorescences of twenty accessions were collected and fixed in a solution of ethanol:acetic acid(3:1)for 24 hours,then stored in ethanol 70%under refrigeration.Six slides per plant,two for each stain,were prepared by squashing,and 300 pollen grains per slide were analyzed.Pollen viability was high(>70%)for most accessions of P.paniculata using the Alexander’s stain,which proved the most adequate method to estimate pollen viability.
文摘Aim of study: Ancient literature alluded to the use of a number of plants/preparations as sex enhancer. One of such botanicals is Phoenix dactylifera in which the pollen grain has been acclaimed to be used as an aphrodisiac. However, the validity has not been scientifically tested. Dopamine is known to facilitate male sexual function. Therefore, the present study was undertaken to evaluate the effect of aqueous extract of Phoenix dactylifera pollen on the sexual behavior of male rats and to measure of serum Estradiol and Testostrone. Also, dopamine transmission in the nucleus accumbence (NAc) was studied in male rats using in vivo microdialysis. Methods and Materials: sixty male rats were randomized into 6 groups (A-F). Group A received 0.2 ml of Normal Saline mixed with Dimethyl Sulphate (DMSO), while groups B-F were injected same volume containing 35 mg/kg, 70 mg/kg, 105 mg/kg, 140 mg/kg and 350 mg/kg of DPP extract, respectively. Sexual behavioral parameters including mounting, intromission and ejaculation frequencies and latencies were recorded in male rats one hour after injection of extract by mating with a receptive female (1:1). The male serum testosterone and estradiol concentrations were also determined. Results: All doses stimulated male sexual behavior. Extract significantly increased mount, ejaculation, intromission frequencies and ejaculation latency in comparison to controlled ones (p 0.001). Mount and intromission latencies significantly reduced (p 0.001). Maximum effect was observed in dose 140 mg/kg. This extract was found to enhance Testestrone, Estradiol and the orientation of males toward female ones by increasing mounting and ano-genital investigatory behavior. Conclusions: Data from this study identified that the aqueous extract of Phoenix dactylifera pollen grain enhanced sexual behaviour in male rats. The improved sexual appetitive behaviour in male rats may be attributed, to the alkaloids, saponins, and or flavonoids since these phytochemicals has engorgement, androgen enhancing. Also, our findings support the traditional use of this plant as acclaimed aphrodisiac and for the treatment of pre-ejaculation and impotency.
文摘The aim of the current study was to investigate the process of microgametogenesis and diagnostic of condition of the Pinus sylvestris male generative system in the tree stands exposed to the influence of Reftinskiy GRES power plant (RGpp) emission during ontogenesis in connection with the level of technogenic pollution. It was detected that in the impact area, smoke emissions from the RGpp in the south-west direction zones of technogenic pollution were formed. Those vary in qualitative composition of the snow pack pollutants and their quantitative characteristics. The content of water insoluble compounds in melt snow water and the compile suspended matter, decreases regularly at the longer distance from the source of air pollutants. The highest content of water soluble compounds in the melt snow water was indicated at the distances of 5, 8 and 14 km from the source of air pollutants, authentically lower in the nearest and farthest sampling points of the snow trials. Scots pine (Pinus sylvestris) pollen significative with high prognostic value in relation to the level of toxic load upon the eco-systems and the condition of male generative system were discovered. It was detected that the significative meanings of pollen with morphological abnormalities decreased, and the meanings of functional significatives increased at the longer distance from the source of air pollutants. Pollen fertility, pollen tube length and reserve substances content significative is negatively related to the presence of micro and macro elements in the snow water;those identify the toxic load level on the ecosystem probably to the highest degree. Pine male gametophyte elimination, while forming in the impact zone of the GRES power plant, takes place at the stage of pollen grain germination as well as pollen tubes’ growth stage.
文摘A medicolegal/botanical collaboration has to lead to an interdisciplinary procedure for developing methods when studying dead bodies found in water with no clear circumstances. Hereby we present a report or a preliminary attempt as a basis for encouraging further work in the field. The body of an unidentified 25 - 35 years old woman was found immersed in a fresh water ditch in state of decomposition. Structural alterations avoid any a priori identification of asphyxia or traumatic cervical lesions. The last cause of death was estimated as a cardio respiratory collapse and a drowning investigation was proposed. Taxonomic species identification was carried out to relate the pollen aspiration with the water medium in lung, spleen, liver and water medium. We presented pollen grains in spleen, as a closed organ, as a clue for death investigation in the absence of diatom findings. Information about where and when pollen grains occur may ascertain that a body or an object was in some place at a certain time. Since the consequences of aspiration depend on many factors, such as the quantity, size and solidity of the material aspirated and whether the aspiration is chronically recurrent or acute, the pollen investigation must be done with precaution. We conclude that in the absence of other microscopic structures, biochemical blood analyses, or histological findings, forensic palynology may be of help to relate the presumptive drowned body with its death scenario.
文摘良渚遗址群是距杭州市西北18 km的良渚镇和瓶窑镇毗邻地带间许多遗址的总称,代表了良渚文化发展的最高成就,是良渚文化的中心。根据在2007年发现的良渚古城墙外侧开挖的良渚北剖面、良渚西剖面沉积样品的粒度分析、孢粉分析数据,以及综合区域内多个剖面地层对比,恢复了该地区全新世环境的演变过程。研究表明,全新世良渚遗址群内环境演变可以分为4个阶段：（1）7 ka BP以前的平原区多为静水沉积环境,水域范围较大,水位较高,且水深逐渐增加,不太适合人类活动;（2）7~5.1 ka BP区域水位有所下降,水域面积缩小,部分较高的地区露出水面,较少接受沉积,一些低洼地区接受了沼泽相或河流泛滥相沉积,一些人类活动遗址开始出现;（3）5.1~4.3 ka BP水位较低,文化快速发展,平原上多见良渚文化遗迹,一些遗址堆积了较厚的良渚文化层;（4）4.3 ka BP以后水位再次升高,良渚文化衰落,多数良渚文化层之上都覆盖一套黄色或黄褐色泛滥相沉积。该地区全新世时期的水位波动与人类文化兴衰演化历史相关的现象表明,良渚遗址区域内地貌特征及水文环境的变化是影响良渚文化兴衰演化的重要原因之一。
