Study on Growth and Decline Law of Contagious Pleuro-pneumonia in Piglets

In order to establish the immune procedure for Actinobacillus pleuropneumonia and to determine the date for the first immunization on piglets, the growth and decline law of A. pleuropneumonia material antibodies in the piglets borne by cows inoculated with A. pleuropneumonia vaccines （type I, II, and VII） before delivery was detected. The results showed that type I, II, and VII maternal antibodies in piglets decreased gradually with the age growing overall, and was at the critical protection value at the ages of 42-50 days （type I） and 28 days （type VII）, lower than the quantification rate; and the antibodies all turned to be negative until the ages of 70 days （type I）, 60 days （type VII） and 35 days （type II）. The first immunization should be carried out at the age of 42-50 days using type I A. pleuropneumonia vaccine, and at the age of 28 days using type VII A. pleuropneumonia vaccine. However, type II A. pleuropneumonia maternal antibody had lower level and positive rate and could not well protect piglets, so the various A. pleuropneumonia vaccines differed in the date for the first immunization. In order to achieve a better immunization effect, A. pleuropneumonia vaccines with different valences should be further researched and developed.

Shale oil enrichment evaluation and production law in Gulong Sag,Songliao Basin,NE China

Based on the results of drilling,tests and simulation experiments,the shales of the Cretaceous Qingshankou Formation in the Gulong Sag of the Songliao Basin are discussed with respect to hydrocarbon generation evolution,shale oil occurrence,and pore/fracture evolution mechanism.In conjunction with a substantial amount of oil testing and production data,the Gulong shale oil enrichment layers are evaluated and the production behaviors and decline law are analyzed.The results are drawn in four aspects.First,the Gulong shales are in the stage of extensive hydrocarbon expulsion when R_(0) is 1.0%-1.2%,with the peak hydrocarbon expulsion efficiency of 49.5%approximately.In the low-medium maturity stage,shale oil migrates from kerogen to rocks and organic pores/fractures.In the medium-high maturity stage,shale oil transforms from adsorbed state to free state.Second,the clay mineral intergranular pores/fractures,dissolution pores,and organic pores make up the majority of the pore structure.During the transformation,clay minerals undergo significant intergranular pore/fracture development between the minerals such as illite and illite/smectite mixed layer.A network of pores/fractures is formed by organic matter cracking.Third,free hydrocarbon content,effective porosity,total porosity,and brittle mineral content are the core indicators for the evaluation of shale oil enrichment layers.Class-I layers are defined as free hydrocarbon content equal or greater than 6.0 mg/g,effective porosity equal or greater than 3.5%,total porosity equal or greater than 8.0%,and brittle mineral content equal or greater than 50%.It is believed that the favourable oil layers are Q2-Q3 and Q8-Q9.Fourth,the horizontal wells in the core area of the light oil zone exhibit a high cumulative production in the first year,and present a hyperbolic production decline pattern,with the decline index of 0.85-0.95,the first-year decline rate of 14.5%-26.5%,and the single-well estimated ultimate recovery(EUR)greater than 2.0×10^(4)t.In practical exploration and production,more efforts will be devoted to the clarification of hydrocarbon generation and expulsion mechanisms,accurate testing of porosity and hydrocarbon content/phase of shale under formation conditions,precise delineation of the boundary of enrichment area,relationship between mechanical properties and stimulated reservoir volume,and enhanced oil recovery,in order to improve the EUR and achieve a large-scale,efficient development of shale oil.