A mechanism to improve early diabetic nephropathy by modulating autophagy-related mechanisms with Yuye Decotion

Objective: To investigate the molecular mechanisms of Yuye Decotion in the treatment of diabetic kidney disease using network pharmacology methods and molecular docking techniques. Methods: Obtain the transcriptome gene expression of diabetic nephropathy through GEO database, and extract genes related to autophagy. Screen the active ingredients and corresponding targets of Yuye Decoction through the TCMSP database, and map the drug prediction targets and disease targets to obtain the autophagy-related Yuye treatment targets for diabetic nephropathy point. Use String database combined with Cytoscape 3.7.2 software to construct the "drug-active ingredient-target" network and protein interaction network of Yuyetang for the treatment of diabetic nephropathy. The target point of liquid soup in the treatment of diabetic nephropathy was analyzed by GO biological process enrichment analysis and KEGG pathway enrichment analysis, and finally used Pymol and other software to analyze the core active components of Yuye Decotion and The core target protein undergoes molecular docking verification. Results: (i)100 eligible diabetic nephropathy and autophagy related genes were screened, and the potential targets of Yuye Decoction were 1,428. The acquired genes related to diabetic nephropathy and autophagy were mapped to potential targets of Yuye Decoction, and 22 therapeutic targets were obtained. GO biological process enrichment analysis and KEGG pathway enrichment analysis found that the pathways related to autophagy in the treatment of diabetic nephropathy by Yuye Decoction may include mTOR signaling pathway, phospholipase D signaling pathway, insulin resistance, EGFR tyrosine kinase inhibitor resistance, Apoptosis, PI3K /Akt signaling pathway, NF-κB signaling pathway, etc. (ii)The protein interaction network shows that VEGFA, ERBB2, GASP3, MAPK8, MYC, CDKN1A, EGFR, BCL2L1 may be the key targets of Yuye Decoction in the treatment of diabetic nephropathy. Molecular docking realizes the binding of 4 core active ingredients to 8 core target proteins. Conclusions: The research results show that Yuye Decoction treats diabetic nephropathy through multi-component, multi-target, and multi-pathway action, and provides new theoretical basis for the study of pharmacological effects and clinical application of Yuye Decoction in the treatment of diabetic nephropathy in autophagy-related aspects.

Clinical Study on Rexiao decotion for asthma of heat wheezing type at acute stage

Objective:To study the safety,efficacy and mechanism of Wang Yulin self-made recipe Rexiao decoction in the treatment of acute bronchial asthma(BA).Methods:80 patients with acute attack BA were randomly divided into western medicine group(n=40)and traditional Chinese and western medicine group(n=40).The control group was treated with routine western medicine:salmeterol was inhaled twice a day,combined with oxygen therapy and inducement therapy.The Chinese and Western medical group was treated with Rexiao decoction in the western medical group.The course of treatment is 15 days.Their syndrome score,blood gas analysis(PaC02,Pa02)and induced sputum IL-5,EOS,IL-1 were observed.3.Pulmonary function(record of kidney,liver function,blood routine and adverse reactions in PEF%Pred,FEV1%Pred),group).Results:the total effective rate was 85%in western medicine group and 92.5%in traditional Chinese and western medicine group(P<0.01).After treatment,the score of TCM syndromes in the Chinese and western medicine group was better than that in the western medicine group(P<0.05).After treatment,the pulmonary function(FEV1%Pred)of the traditional Chinese and western medicine group was better than that of the western medicine group,and the PEF%Pred of the Chinese and western medicine group was better than that of the western medicine group.Analysis of Blood Gas in traditional Chinese and Western Medicine Group after treatment(PaO2)was significantly higher than that in the control group(P<0.05).There was no significant difference in the improvement of blood gas analysis(PaCO2)between the western medicine group and the traditional Chinese and western medicine group(P>0.05).The decrease of IL-13,EOS and IL-5 in sputum of traditional Chinese and western medicine group was more than that of western medicine group(P<0.01).There was no significant difference in liver function(AST,ALT),renal function(Cr,Urea),blood routine(WBC,RBC,PLT)before and after treatment in traditional Chinese and western medicine group(P>0.05),but no obvious adverse reactions were found.Conclusions:heat Asthma decoction is safe and effective in the treatment of hot asthma syndrome in acute attack.The target and inflammatory factors,eosinophils are likely to decrease,and the effect of airway inflammation and high reaction are decreased.

生化汤对剖宫产术后子宫复旧作用的临床观察

目的探讨加味生化汤对剖宫产术后患者子宫复旧的治疗作用。方法对120例剖宫产术后患者随机分为对照组60例,采用缩宫素治疗;观察组60例,加用加味生化汤治疗。计算宫底下降速度,观察恶露持续时间,产后42天宫腔积血发生率。结果 120例全部进入结果分析。观察组宫底高度下降速度较对照组快,两组比较差异有极显著性意义(P<0.01);观察组恶露持续时间明显缩短(P<0.01);产后42天两组子宫三径对比,观察组恢复好,但与对照组相比差别统计学无显著性意义(P>0.05),宫腔积血发生率,观察组为5%,对照组为11.7%。结论加味生化汤可加快产后子宫底下降速度,缩短恶露持续时间,降低产后宫腔积血发生率。

Professor Xiaoyan Wang used Yinhuo Decoction to Treat Yang Insomnia from not Entering Yin Type

Yinhuo decoction is recorded by Shiduo Chen,a doctor in the Qing Dynasty,in the Dialectical Record.It is mainly used to treat4*"tonsillitis with yin asthenia.^^Its pathogenesis is deficiency of kidney Yin and Yang-function insufficiency of kidney Zang,resulting in vacuity heat floating upward.Professor Xiaoyan Wang,the tutor,believes that the general pathogenesis of insomnia is that Vang does not enter Yin.The application of Yinhuo Decoction in clinic to treat insomnia is quite effective.

Bawu Decoction(八物汤) Ameliorates Benign Prostatic Hyperplasia in Rats

Objective：To evaluate the efficacy of Bawu Decoction（八物汤,BWD,Palmul-tang in Korean）against benign prostatic hyperplasia（BPH）.Methods：Twenty-four male Wistar rats were divided into 4 groups,with 6 rats in each group.The 4 study groups included sham-operated group（CON）,BPH model group,finasteride-treated group,and BWD-treated group.All the groups except CON group received a subcutaneous injection of 10 mg/kg of testosterone,while CON group received saline.Finasteride at a dose of 5 mg/kg was administered to the finasteride-treated group for a period of 4 weeks.BWD group received BWD at a dose of 200 mg/kg for 4 weeks.The prostatic weight,prostate weight to body weight ratio,relative prostate weight ratio,serum testosterone and dihydrotestosterone（DHT）level,and histological analysis of prostatic tissue were analyzed.Results：Compared to BPH model group,BWD administration was associated with reductions in prostatic weight,prostate and relative prostate weight ratio weight to body weight ratio（P〈0.05）.The concentration of serum testosterone and DHT were higher in BPH group compared with CON group（P〈0.05）.Administration of finasteride and BWD suppressed the elevation of serum testosterone and DHT levels significantly（both P〈0.05）.In addition,BWD suppressed the growth of prostatic tissue（P〈0.05）.Conclusion：BWD has suppressant effects on development of BPH through inhibition of serum testosterone and DHT.