Objective:To explore the effects of 6-gingerol,asarinin,and deoxyschizandrindthe main components of Zingiber officinale(Willd.)Rosc.(Gan Jiang),Asarum heterotropoides f.var.mandshuricum(Maxim.)(Xi Xin),and Schisandra ...Objective:To explore the effects of 6-gingerol,asarinin,and deoxyschizandrindthe main components of Zingiber officinale(Willd.)Rosc.(Gan Jiang),Asarum heterotropoides f.var.mandshuricum(Maxim.)(Xi Xin),and Schisandra chinensis(Turcz.)Baill.(Wu Wei Zi),respectivelydon an interleukin(IL)-13einduced BEAS-2B cell model in vitro.Methods:The BEAS-2B cell model was established using 25 ng/mL IL-13 combined with 1%fetal bovine serum(FBS)in vitro.Mitoquinone mesylate(Mito-Q)treatment was used as a positive control group,and different concentrations of 6-gingerol,asarinin,and deoxyschizandrin were used to treat the models.The level of reactive oxygen species(ROS)production was detected by flow cytometry.The expression levels of LC3B,Beclin1,adenosine 50-monophosphate(AMP)eactivated protein kinase(AMPK),phosphory-lated-AMPeactivated protein kinase(P-AMPK),dynamin-related protein 1(DRP1),and mitochondrial fusion protein 2(MFN2)were detected by Western blot.Mitochondrial membrane potential(MMP)assay kit with JC-1 was utilized to detect the level of MMP.Results:The BEAS-2B cells exposed to 25 ng/mL IL-13 with 1%FBS showed an increased ROS level and a decreased MMP.6-Gingerol,asarinin,and deoxyschizandrin were able to downregulate ROS level and upregulate the MMP in the BEAS-2B model.Asarinin and deoxyschizandrin reduced the expression of autophagy protein LC3B,while deoxyschizandrin significantly increased the expression of DRP1 in the BEAS-2B model.Conclusion:6-Gingerol,asarinin,and deoxyschizandrin can reduce ROS generation and increase MMP,but have different regulatory effects on the expression of autophagy protein and mitochondrial mitotic protein.The three components have both synergistic and complementary effects in classic medicine compatibility.This study may provide an innovative strategy to reduce the lung inflammation related to IL-13.展开更多
A high-speed counter-current chromatography(HSCCC)method was successfully developed for the preparative separation and purification of deoxyschizandrin from Schisandrae Sphenantherae Fructus in one step.The purity of ...A high-speed counter-current chromatography(HSCCC)method was successfully developed for the preparative separation and purification of deoxyschizandrin from Schisandrae Sphenantherae Fructus in one step.The purity of deoxyschizandrin was 98.5%,and the structure was identified by MS,UV and NMR.This method was simple,fast,convenient and appropriate to prepare pure compound as reference substances for related research on Schisandrae Sphenantherae Fructus.展开更多
DEOXYSCHIZANDRIN 10,isolated from Schizandra chinensis baill,exhibits anti-hepatitisvirus activity.Recently considerable interest in the synthesis of 10 has arisen from its bioactiv-ities.In this note a new highly eff...DEOXYSCHIZANDRIN 10,isolated from Schizandra chinensis baill,exhibits anti-hepatitisvirus activity.Recently considerable interest in the synthesis of 10 has arisen from its bioactiv-ities.In this note a new highly efficient route to synthesize(±)-deoxyschizandrin is re-ported.Our synthetic strategy(outlined in scheme 1)started from gallic acid 1 in 10 stepswith the overall yield of 12%,and employed I<sub>2</sub>/NaOEt oxidative coupling reaction as a展开更多
目的:研究五味子甲素对人肝星状细胞系LX-2细胞的增殖、纤维化指标及凋亡相关蛋白的影响。方法:采用不同浓度的五味子甲素与肝星状细胞LX-2共孵育,MTT法检测五味子甲素对细胞的抑制作用,RT-PCR法和Western blot法测定纤维化指标I型胶原...目的:研究五味子甲素对人肝星状细胞系LX-2细胞的增殖、纤维化指标及凋亡相关蛋白的影响。方法:采用不同浓度的五味子甲素与肝星状细胞LX-2共孵育,MTT法检测五味子甲素对细胞的抑制作用,RT-PCR法和Western blot法测定纤维化指标I型胶原α亚基1(collagen type I alpha 1,collagen I-1)和α-平滑肌动蛋白(α-smooth muscle actin,α-SMA)的表达量,并检测五味子甲素对凋亡相关蛋白Bcl-2和Bax表达量的影响。结果:五味子甲素在不同浓度时可抑制人肝星状细胞系LX-2细胞的增殖,可降低collagenⅠ和α-SMA的mRNA水平和蛋白表达量(P<0.05),并能降低Bcl-2的mRNA水平和蛋白表达量以及Bcl-2/Bax的比值(P<0.05)。结论:五味子甲素可能通过抑制细胞增殖、降低纤维化指标的表达,从而发挥抗肝纤维化的作用。展开更多
Deoxyschizandrin was extracted from Schisandra incarnata by methanol reflux. The extraction processand RP-HPLC separation conditions were optimized. The optimal extraction conditions were as follows: nine volumes of80...Deoxyschizandrin was extracted from Schisandra incarnata by methanol reflux. The extraction processand RP-HPLC separation conditions were optimized. The optimal extraction conditions were as follows: nine volumes of80% methanol reflux 120min, for two times. A C18 column was used with the mobile phase of methanol-water(85∶15)atthe detection wavelength of 220nm. The column temperature was 30℃ with the flow rate of 1ml/min. The average contentof deoxyschizandrin in Schisandra incarnata was 0.22%.展开更多
基金This study was supported by the National Natural Science Foundation of China(81403313)the Vertical Development Fund of Beijing University of Chinese Medicine(2019-ZXFZJJ-062).
文摘Objective:To explore the effects of 6-gingerol,asarinin,and deoxyschizandrindthe main components of Zingiber officinale(Willd.)Rosc.(Gan Jiang),Asarum heterotropoides f.var.mandshuricum(Maxim.)(Xi Xin),and Schisandra chinensis(Turcz.)Baill.(Wu Wei Zi),respectivelydon an interleukin(IL)-13einduced BEAS-2B cell model in vitro.Methods:The BEAS-2B cell model was established using 25 ng/mL IL-13 combined with 1%fetal bovine serum(FBS)in vitro.Mitoquinone mesylate(Mito-Q)treatment was used as a positive control group,and different concentrations of 6-gingerol,asarinin,and deoxyschizandrin were used to treat the models.The level of reactive oxygen species(ROS)production was detected by flow cytometry.The expression levels of LC3B,Beclin1,adenosine 50-monophosphate(AMP)eactivated protein kinase(AMPK),phosphory-lated-AMPeactivated protein kinase(P-AMPK),dynamin-related protein 1(DRP1),and mitochondrial fusion protein 2(MFN2)were detected by Western blot.Mitochondrial membrane potential(MMP)assay kit with JC-1 was utilized to detect the level of MMP.Results:The BEAS-2B cells exposed to 25 ng/mL IL-13 with 1%FBS showed an increased ROS level and a decreased MMP.6-Gingerol,asarinin,and deoxyschizandrin were able to downregulate ROS level and upregulate the MMP in the BEAS-2B model.Asarinin and deoxyschizandrin reduced the expression of autophagy protein LC3B,while deoxyschizandrin significantly increased the expression of DRP1 in the BEAS-2B model.Conclusion:6-Gingerol,asarinin,and deoxyschizandrin can reduce ROS generation and increase MMP,but have different regulatory effects on the expression of autophagy protein and mitochondrial mitotic protein.The three components have both synergistic and complementary effects in classic medicine compatibility.This study may provide an innovative strategy to reduce the lung inflammation related to IL-13.
基金supported by the International Scientific and Technological Cooperation Program of China(No.2009DFA31230)the Industry-University-Research Cooperation Program from Science and Technology Department of Guangdong Province(No.2010B090400533)
文摘A high-speed counter-current chromatography(HSCCC)method was successfully developed for the preparative separation and purification of deoxyschizandrin from Schisandrae Sphenantherae Fructus in one step.The purity of deoxyschizandrin was 98.5%,and the structure was identified by MS,UV and NMR.This method was simple,fast,convenient and appropriate to prepare pure compound as reference substances for related research on Schisandrae Sphenantherae Fructus.
文摘DEOXYSCHIZANDRIN 10,isolated from Schizandra chinensis baill,exhibits anti-hepatitisvirus activity.Recently considerable interest in the synthesis of 10 has arisen from its bioactiv-ities.In this note a new highly efficient route to synthesize(±)-deoxyschizandrin is re-ported.Our synthetic strategy(outlined in scheme 1)started from gallic acid 1 in 10 stepswith the overall yield of 12%,and employed I<sub>2</sub>/NaOEt oxidative coupling reaction as a
文摘目的:研究五味子甲素对人肝星状细胞系LX-2细胞的增殖、纤维化指标及凋亡相关蛋白的影响。方法:采用不同浓度的五味子甲素与肝星状细胞LX-2共孵育,MTT法检测五味子甲素对细胞的抑制作用,RT-PCR法和Western blot法测定纤维化指标I型胶原α亚基1(collagen type I alpha 1,collagen I-1)和α-平滑肌动蛋白(α-smooth muscle actin,α-SMA)的表达量,并检测五味子甲素对凋亡相关蛋白Bcl-2和Bax表达量的影响。结果:五味子甲素在不同浓度时可抑制人肝星状细胞系LX-2细胞的增殖,可降低collagenⅠ和α-SMA的mRNA水平和蛋白表达量(P<0.05),并能降低Bcl-2的mRNA水平和蛋白表达量以及Bcl-2/Bax的比值(P<0.05)。结论:五味子甲素可能通过抑制细胞增殖、降低纤维化指标的表达,从而发挥抗肝纤维化的作用。
文摘Deoxyschizandrin was extracted from Schisandra incarnata by methanol reflux. The extraction processand RP-HPLC separation conditions were optimized. The optimal extraction conditions were as follows: nine volumes of80% methanol reflux 120min, for two times. A C18 column was used with the mobile phase of methanol-water(85∶15)atthe detection wavelength of 220nm. The column temperature was 30℃ with the flow rate of 1ml/min. The average contentof deoxyschizandrin in Schisandra incarnata was 0.22%.