Structured illumination microscopy(SIM)is one of the most widely applied wide field super resolution imaging techniques with high temporal resolution and low phototoxicity.The spatial resolution of SIM is typically li...Structured illumination microscopy(SIM)is one of the most widely applied wide field super resolution imaging techniques with high temporal resolution and low phototoxicity.The spatial resolution of SIM is typically limited to two times of the diffraction limit and the depth of field is small.In this work,we propose and experimentally demonstrate a low cost,easy to implement,novel technique called speckle structured illumination endoscopy(SSIE)to enhance the resolution of a wide field endoscope with large depth of field.Here,speckle patterns are used to excite objects on the sample which is then followed by a blind-SIM algorithm for super resolution image reconstruction.Our approach is insensitive to the 3D morphology of the specimen,or the deformation of illuminations used.It greatly simplifies the experimental setup as there are no calibration protocols and no stringent control of illumination patterns nor focusing optics.We demonstrate that the SSIE can enhance the resolution 2–4.5 times that of a standard white light endoscopic(WLE)system.The SSIE presents a unique route to super resolution in endoscopic imaging at wide field of view and depth of field,which might be beneficial to the practice of clinical endoscopy.展开更多
The point spread function(PSF)caused by a wavefront coding system with a cubic phase mask has big side-lobes which leads to bad image restoration.This paper proposes a novel apodized cubic phase mask to suppress the s...The point spread function(PSF)caused by a wavefront coding system with a cubic phase mask has big side-lobes which leads to bad image restoration.This paper proposes a novel apodized cubic phase mask to suppress the side-lobes of the PSF.Simulated annealing algorithm is used to optimize the cubic and the truncation parameter of the phase mask.The system with the novel phase mask has better performance in the modulation transfer function(MTF)especially in low-and-medium spatial frequency region.The simulation results show that the restored images with the novel phase mask are superior to the one with the classic cubic phase mask in contrast and ringing effect.The experimental results show that the side-lobes of the PSF are suppressed by using the apodized cubic phase mask.展开更多
We propose optical experiments to study the depth of field for a thermal light lensless ghost imaging system. It is proved that the diaphragm is an important factor to influence the depth of field, and the ghost image...We propose optical experiments to study the depth of field for a thermal light lensless ghost imaging system. It is proved that the diaphragm is an important factor to influence the depth of field, and the ghost images of two detected objects with longitudinal distance less than the depth of field can be achieved simultaneously. The longitudinal coherence scale of the thermal light lensless ghost imaging determines the depth of field. Theoretical analysis can well explain the experimental results.展开更多
A concept of divergence angle of light beams(DALB)is proposed to analyze the depth of field(DOF)of a 3D light-field display system.The mathematical model between DOF and DALB is established,and the conclusion that DOF...A concept of divergence angle of light beams(DALB)is proposed to analyze the depth of field(DOF)of a 3D light-field display system.The mathematical model between DOF and DALB is established,and the conclusion that DOF and DALB are inversely proportional is drawn.To reduce DALB and generate clear depth perception,a triple composite aspheric lens structure with a viewing angle of 100°is designed and experimentally demonstrated.The DALB-constrained 3D light-field display system significantly improves the clarity of 3D images and also performs well in imaging at a 3D scene with a DOF over 30 cm.展开更多
A key limitation in the observation of instruments used in operations and heart sutures during a procedure is the scattering and absorption during optical imaging in the presence of blood.Therefore,we propose a novel ...A key limitation in the observation of instruments used in operations and heart sutures during a procedure is the scattering and absorption during optical imaging in the presence of blood.Therefore,we propose a novel real-time fiber-optic infrared imaging system simultaneously capturing a flexible wide field of view(FOV) and large depth of field infrared image in real time.The assessment criteria for imaging quality of the objective and coupling lens have been optimized and evaluated.Furthermore,the feasibility of manufacturing and assembly has been demonstrated with tolerance sensitivity and the Monte Carlo analysis.The simulated results show that the optical system can achieve a large working distance of 8 to25 mm,a wide FOV of 120°,and the relative illuminance is over 0.98 in the overall FOV.To achieve high imaging quality in the proposed system,the modulation transfer function is over 0.661 at 16.7 Ip/mm for a 320×256 short wavelength infrared camera sensor with a pixel size of 30 μm.展开更多
Objective:As traditional techniques for microscopic identification of Chinese medicines currently lack objective and high-quality reference images,here we developed a systemic procedure to be used in microscopic ident...Objective:As traditional techniques for microscopic identification of Chinese medicines currently lack objective and high-quality reference images,here we developed a systemic procedure to be used in microscopic identification of Chinese medicines,which would lead to more objective,effective and accurate identification process.Methods:Spatholobi Caulis(Jixueteng in Chinese)was used as the specimen in the development of such procedure.Jixueteng samples were microscopically examined in bright-and dark-field microscopy.Microscopic images were obtained by regular,EDF,and image stitching techniques.Results:The microscopic images of the characteristics in pulverized Jixueteng were captured,thanks to EDF imaging and image stitching techniques which allowed the detailed and full sighting of each characteristic to be obtained simultaneously.Different layers in anatomical transverse section,including cork,phelloderm,cortex,phloem,cambium,xylem and pith,were distinctively observed.Moreover,by comparing images of bright-and dark-field microscopy,birefringent and non-birefringent components could readily be distinguished.Conclusion:With application of the developed procedure,high-definition,panoramic and microscopic images were acquired,which could be used as the reference images for microscopic identification of Chinese medicines.展开更多
Tracking moving particles in cells by single particle tracking is an important optical approach widely used in biological research. In order to track multiple particles within a whole cell simultaneously, a parallel t...Tracking moving particles in cells by single particle tracking is an important optical approach widely used in biological research. In order to track multiple particles within a whole cell simultaneously, a parallel tracking approach with large depth of field was put forward. It was based on distorted grating and dual-objective bifocal imaging, making use of the distorted grating to expand the depth of field, dual-objective to gather as many photons as possible, and bifocal plane imaging to realize three-dimensional localization. Simulation of parallel tracking of two particles moving along the z axis demonstrated that even when the two are axially separated by10 μm, they can both be localized simultaneously with transversal precision better than 5 nm and axial precision better than 20 nm.展开更多
Background:Presbyopia has become a global disease affecting the world's aging population.Among various treatments,cataract extraction and intraocular lens(IOL)implantation have become the most popular and common m...Background:Presbyopia has become a global disease affecting the world's aging population.Among various treatments,cataract extraction and intraocular lens(IOL)implantation have become the most popular and common methods of presbyopia correction.During the twentieth century,IOLs have underwent significant innovation and advancements to meet the patients'high demands for functional vision at all distances.Main Text:To meet the increasing needs for excellent near and intermediate vision for daily activities,some premium IOLs with more than one focus have been developed,for example,the refractive MfIOLs,diffractive MfIOLs,extended depth of field(EDOF)IOLs,and accommodating IOLs(AIOLs)were introduced to meet this need.In addition,the add-on MfIOLs have been explored as promising supplementary IOLs for pseudophakic presbyopia.When selecting the MfIOLs,the IOLs'features,patients’characteristics,preoperative eye conditions,and treatment expectations should be considered.Conclusions:In this review,we focus on the multifocal IOLs(MfIOLs)commonly used for presbyopia correction and systematically summarized their optical designs and clinical outcomes.More evidence-based studies are required to provide guidelines for MfIOL selection,provide maximum visual benefits,and develop personalized visual solutions in the future.展开更多
基金partially supported by the Gordon and Betty Moore Foundation Grant No.5722
文摘Structured illumination microscopy(SIM)is one of the most widely applied wide field super resolution imaging techniques with high temporal resolution and low phototoxicity.The spatial resolution of SIM is typically limited to two times of the diffraction limit and the depth of field is small.In this work,we propose and experimentally demonstrate a low cost,easy to implement,novel technique called speckle structured illumination endoscopy(SSIE)to enhance the resolution of a wide field endoscope with large depth of field.Here,speckle patterns are used to excite objects on the sample which is then followed by a blind-SIM algorithm for super resolution image reconstruction.Our approach is insensitive to the 3D morphology of the specimen,or the deformation of illuminations used.It greatly simplifies the experimental setup as there are no calibration protocols and no stringent control of illumination patterns nor focusing optics.We demonstrate that the SSIE can enhance the resolution 2–4.5 times that of a standard white light endoscopic(WLE)system.The SSIE presents a unique route to super resolution in endoscopic imaging at wide field of view and depth of field,which might be beneficial to the practice of clinical endoscopy.
文摘The point spread function(PSF)caused by a wavefront coding system with a cubic phase mask has big side-lobes which leads to bad image restoration.This paper proposes a novel apodized cubic phase mask to suppress the side-lobes of the PSF.Simulated annealing algorithm is used to optimize the cubic and the truncation parameter of the phase mask.The system with the novel phase mask has better performance in the modulation transfer function(MTF)especially in low-and-medium spatial frequency region.The simulation results show that the restored images with the novel phase mask are superior to the one with the classic cubic phase mask in contrast and ringing effect.The experimental results show that the side-lobes of the PSF are suppressed by using the apodized cubic phase mask.
基金Supported by the Beijing Natural Science Foundation under Grant No 4133086the Fundamental Research Funds for th Central Universities under Grant No 2-9-2014-022
文摘We propose optical experiments to study the depth of field for a thermal light lensless ghost imaging system. It is proved that the diaphragm is an important factor to influence the depth of field, and the ghost images of two detected objects with longitudinal distance less than the depth of field can be achieved simultaneously. The longitudinal coherence scale of the thermal light lensless ghost imaging determines the depth of field. Theoretical analysis can well explain the experimental results.
基金supported by the National Key Research and Development Program of China(No.2021YFB3600504)the National Natural Science Foundation of China(Nos.62175015 and 62075016)。
文摘A concept of divergence angle of light beams(DALB)is proposed to analyze the depth of field(DOF)of a 3D light-field display system.The mathematical model between DOF and DALB is established,and the conclusion that DOF and DALB are inversely proportional is drawn.To reduce DALB and generate clear depth perception,a triple composite aspheric lens structure with a viewing angle of 100°is designed and experimentally demonstrated.The DALB-constrained 3D light-field display system significantly improves the clarity of 3D images and also performs well in imaging at a 3D scene with a DOF over 30 cm.
基金supported by the Shanghai Science and Technology Committee Innovation Grant (No. 19ZR1404600)the National Natural Science Foundation of China (No. 52075100)
文摘A key limitation in the observation of instruments used in operations and heart sutures during a procedure is the scattering and absorption during optical imaging in the presence of blood.Therefore,we propose a novel real-time fiber-optic infrared imaging system simultaneously capturing a flexible wide field of view(FOV) and large depth of field infrared image in real time.The assessment criteria for imaging quality of the objective and coupling lens have been optimized and evaluated.Furthermore,the feasibility of manufacturing and assembly has been demonstrated with tolerance sensitivity and the Monte Carlo analysis.The simulated results show that the optical system can achieve a large working distance of 8 to25 mm,a wide FOV of 120°,and the relative illuminance is over 0.98 in the overall FOV.To achieve high imaging quality in the proposed system,the modulation transfer function is over 0.661 at 16.7 Ip/mm for a 320×256 short wavelength infrared camera sensor with a pixel size of 30 μm.
基金financially supported by National Key R&D Program of China(No.2018YFC1707900)。
文摘Objective:As traditional techniques for microscopic identification of Chinese medicines currently lack objective and high-quality reference images,here we developed a systemic procedure to be used in microscopic identification of Chinese medicines,which would lead to more objective,effective and accurate identification process.Methods:Spatholobi Caulis(Jixueteng in Chinese)was used as the specimen in the development of such procedure.Jixueteng samples were microscopically examined in bright-and dark-field microscopy.Microscopic images were obtained by regular,EDF,and image stitching techniques.Results:The microscopic images of the characteristics in pulverized Jixueteng were captured,thanks to EDF imaging and image stitching techniques which allowed the detailed and full sighting of each characteristic to be obtained simultaneously.Different layers in anatomical transverse section,including cork,phelloderm,cortex,phloem,cambium,xylem and pith,were distinctively observed.Moreover,by comparing images of bright-and dark-field microscopy,birefringent and non-birefringent components could readily be distinguished.Conclusion:With application of the developed procedure,high-definition,panoramic and microscopic images were acquired,which could be used as the reference images for microscopic identification of Chinese medicines.
基金supported by the National Natural Science Foundation of China(Nos.11774242,61975131,61605120,and 61335001)Specially Funded Program on National Key Scientific Instruments and Equipment Development(No.2012YQ15009203)+1 种基金Natural Science Foundation of Guangdong Province(No.2018A030313362)Shenzhen Science and Technology Planning Project(Nos.JCYJ20170818142804605 and JCYJ20170818141701667)
文摘Tracking moving particles in cells by single particle tracking is an important optical approach widely used in biological research. In order to track multiple particles within a whole cell simultaneously, a parallel tracking approach with large depth of field was put forward. It was based on distorted grating and dual-objective bifocal imaging, making use of the distorted grating to expand the depth of field, dual-objective to gather as many photons as possible, and bifocal plane imaging to realize three-dimensional localization. Simulation of parallel tracking of two particles moving along the z axis demonstrated that even when the two are axially separated by10 μm, they can both be localized simultaneously with transversal precision better than 5 nm and axial precision better than 20 nm.
基金the National Natural Science Foundation of China(Grant No.81970779 to Zhu Yanan).
文摘Background:Presbyopia has become a global disease affecting the world's aging population.Among various treatments,cataract extraction and intraocular lens(IOL)implantation have become the most popular and common methods of presbyopia correction.During the twentieth century,IOLs have underwent significant innovation and advancements to meet the patients'high demands for functional vision at all distances.Main Text:To meet the increasing needs for excellent near and intermediate vision for daily activities,some premium IOLs with more than one focus have been developed,for example,the refractive MfIOLs,diffractive MfIOLs,extended depth of field(EDOF)IOLs,and accommodating IOLs(AIOLs)were introduced to meet this need.In addition,the add-on MfIOLs have been explored as promising supplementary IOLs for pseudophakic presbyopia.When selecting the MfIOLs,the IOLs'features,patients’characteristics,preoperative eye conditions,and treatment expectations should be considered.Conclusions:In this review,we focus on the multifocal IOLs(MfIOLs)commonly used for presbyopia correction and systematically summarized their optical designs and clinical outcomes.More evidence-based studies are required to provide guidelines for MfIOL selection,provide maximum visual benefits,and develop personalized visual solutions in the future.