Different operating conditions (e.g. design and off-design) may lead to a significant difference in the hydrodynamics performance of a ship, especially in the total resistance and wake field of ships. This work inve...Different operating conditions (e.g. design and off-design) may lead to a significant difference in the hydrodynamics performance of a ship, especially in the total resistance and wake field of ships. This work investigated the hydrodynamic performance of the well-known KRISO 3600 TEU Container Ship (KCS) under three different operating conditions by means of Particle Image Velocimetry (P/V) and Computational Fluid Dynamics (CFD). The comparison results show that the use of PIV to measure a ship's nominal wake field is an important method which has the advantages of being contactless and highly accurate. Acceptable agreements between the results obtained by the two different methods are achieved. Results indicate that the total resistances of the KCS model under two off-design conditions are 23.88% and 13.92% larger than that under the designed condition, respectively.展开更多
Experimental tests were conducted to evaluate the hydrodynamic performance of an L-type podded propulsor in straight-ahead motion and off-design conditions using an open-water measuring instrument developed by the aut...Experimental tests were conducted to evaluate the hydrodynamic performance of an L-type podded propulsor in straight-ahead motion and off-design conditions using an open-water measuring instrument developed by the authors for podded propulsors, a ship model towing tank, and under water particle image velocimetry (PIV) measurement systems. Under the three types of conditions, the main parameters of an L-type podded propulsor were measured, including the propeller thrust and torque, as well as the thrust, side force, and moment of the whole pod unit.In addition, the flow field on the section between the propeller and the strut was analyzed. Experimental results demonstrate that the dynamic azimuthing rate and direction and the turning direction affect the forces on the propeller and the whole pod unit. Forces are asymmetrically distributed between the left and right azimuthing directions because of the effect of propeller rotation. The findings of this study provide a foundation for further research on L-type podded propulsors.展开更多
Harmful algal blooms(HABs) have led to extensive ecological and environmental issues and huge economic losses.Various HAB control techniques have been developed,and biological methods have been paid more attention.Alg...Harmful algal blooms(HABs) have led to extensive ecological and environmental issues and huge economic losses.Various HAB control techniques have been developed,and biological methods have been paid more attention.Algicidal bacteria is a general designation for bacteria which inhibit algal growth in a direct or indirect manner,and kill or damage the algal cells.A metabolite which is strongly toxic to the dinoflagellate Alexandrium tamarense was produced by strain DH46 of the alga-lysing bacterium Alteromonas sp.The culture conditions were optimized using a single-factor test method.Factors including carbon source,nitrogen source,temperature,initial pH value,rotational speed and salinity were studied.The results showed that the cultivation of the bacteria at 28℃ and 180 r min-1with initial pH 7 and 30 salt contcentration favored both the cell growth and the lysing effect of strain DH46.The optimal medium composition for strain DH46 was determined by means of uniform design experimentation,and the most important components influencing the cell density were tryptone,yeast extract,soluble starch,NaNO3 and MgSO4.When the following culture medium was used(tryptone 14.0g,yeast extract 1.63g,soluble starch 5.0 g,NaNO3 1.6 g,MgSO4 2.3 g in 1L),the largest bacterial dry weight(7.36 g L-1) was obtained,which was an enhancement of 107% compared to the initial medium;and the algal lysis rate was as high as 98.4% which increased nearly 10% after optimization.展开更多
Public vehicle seats are used under diverse conditions, therefore, the design of these seats is desired to be universal design. In the past study, seat swing function used in public vehicle seats, and it was optimized...Public vehicle seats are used under diverse conditions, therefore, the design of these seats is desired to be universal design. In the past study, seat swing function used in public vehicle seats, and it was optimized from the mechanical aspect under diverse conditions. Seat swing function is the function to prevent hip-sliding force, which causes sitting uncomfortable feeling. Recent study clarified that the reduction of blood flow in the legs causes physiological dysfunction, which is one of the causes of Long Flight Thrombosis, and the social issue. Thus, in the design of seat swing function, it is necessary to consider not only mechanical aspects but also physiological aspects. For the universal design of seat swing function considering the physiological aspect (blood flow), the influence of blood flow on seat swing function in diverse conditions was analyzed. The method of this study was measuring blood flow and body pressure distribution on seat cushion of seat swing function under diverse conditions. From the analysis, it was indicated that it was necessary to reduce the compressive force behind the knees of the user conditions; the short physique and the high weight physique in standard sitting posture, for the universal design of seat swing function to prevent reduction of blood flow in the legs.展开更多
[ Objective] This study was to improve the biomass of Navicula tenera, and thus to provide reference for achieving the industrial production of Navicula tenera. [Method] The feasibility of using sewage to cultivate Na...[ Objective] This study was to improve the biomass of Navicula tenera, and thus to provide reference for achieving the industrial production of Navicula tenera. [Method] The feasibility of using sewage to cultivate Navicula tenera was preliminarily investigated based on the consideration of regional characteristics; and a series of culture conditions including the nutrient source of nitrogen(N), phosphorus(P), iron(Fe), silicon( Si ) and the salinity in medium for culturing Navicula tenera, were optimized by single factor test and orthogonal design.[Result] The optimized conditions for cultivating Navicula tenera using sewage are as follows: the water from Xiaoerlou Artificial Lake of Nanjing University of Technology as basic solvent; 360 mg/L urea; 150 mg/L N2HPO4 · 12H2O; 50 mg/L ferric citrate; 2 000 mg/L Na2SiO3 · 9H2O; 2.0 mol/L salinity. Navicula tenera was strongly adaptive to sewage and could well uptake the nutrient sources in the sewage. Under the optimized conditions, the culture cost decreased, and meanwhile the biomass of Navicula tenera reached 4. 766 g/L which is 3.57 multiples over original medium and 1.9 multiples over optimized medium No. 1.[ Conclusion] This study laid a foundation for the combination of culturing Navicula tenera in large scale and sewage treatment.展开更多
We studied how bioflocculants,produced by white-rot fungi,affect flocculation in slime water.Based on a test in an orthogonal design,flocculation conditions were optimized.The results show that flocculation activity i...We studied how bioflocculants,produced by white-rot fungi,affect flocculation in slime water.Based on a test in an orthogonal design,flocculation conditions were optimized.The results show that flocculation activity is at its highest when the following conditions are met:slime water concentration 27.42 g/L;coagulant aid(CaCl_2) mass concentration 5.0 g/L;two-segment stirrings:the first at a stirring speed of 60 r/min for 180 s and the second 180 r/min for 60 s;a pH of 11 and a flocculant concentration of 15 mL/L.The flocculation activity can be up to 98.71%of bioflocculants at the time.Further experiments indicate that most of the flocculation active material is found outside the mycelium cells.This is the extracellular secretion produced by mycelium cells during the fermentation process.This flocculant has strong thermal stability.Many kinds of cations have a flocculation function to assist bioflocculants.This aid-flocculation effect of the divalent cation Ca^(2+) is obvious in the bioflocculant produced by the white-rot fungus.Therefore,this is of great value when applied to control engineering in the battle against water pollution.展开更多
[Objective] This study aimed to increase the expression level of immun- odominant membrane protein gene (Imp) of phytoplasmas in E. coil BL21 (DE3). [Method] On the basis of orthogonal experiment, effects of diffe...[Objective] This study aimed to increase the expression level of immun- odominant membrane protein gene (Imp) of phytoplasmas in E. coil BL21 (DE3). [Method] On the basis of orthogonal experiment, effects of different culture conditions on recombinant bacteria E. coil BL21-pET-28a(+)-Imp were investigated. Based on the obtained optimal culture condition, effects of different induction conditions on the ex- pression level of Imp protein were explored. The expression level of Imp fusion pro- tein was analyzed by using SDS-PAGE and Gene Tools software. [Result] The re- sults showed that the optimal conditions for culture were at 37℃, pH 7.0, with liq- uid volume of 20% and oscillation speed of 200 r/min, for induction were at 37℃ for 6 h, with initial OD600 of about 1.5 and IPTG final concentration of 0.1 mmol/L. [Conclusion] The expression level of Imp achieved 70.98 mg/L under the optimal conditions. Optimized conditions for expression of Imp fusion protein in E. coil were determined.展开更多
Sequential methodology based on the application of three types of experimental designs was used to optimize the fermentation conditions for elastase production from mutant strain ZJUEL31410 of Bacillus licheniformis i...Sequential methodology based on the application of three types of experimental designs was used to optimize the fermentation conditions for elastase production from mutant strain ZJUEL31410 of Bacillus licheniformis in shaking flask cul- tures. The optimal cultivation conditions stimulating the maximal elastase production consist of 220 r/min shaking speed, 25 h fermentation time, 5% (v/v) inoculums volume, 25 ml medium volume in 250 ml Erlenmeyer flask and 18 h seed age. Under the optimized conditions, the predicted maximal elastase activity was 495 U/ml. The application of response surface methodology resulted in a significant enhancement in elastase production. The effects of other factors such as elastin and the growth factor (corn steep flour) on elastase production and cell growth were also investigated in the current study. The elastin had no significant effect on enzyme-improved production. It is still not clear whether the elastin plays a role as a nitrogen source or not. Corn steep flour was verified to be the best and required factor for elastase production and cell growth by Bacillus licheniformis ZJUEL31410.展开更多
An algorithm based on the Boundary Element Method(BEM)is presented for designing the High Skew Propeller(HSP)used in an Underwater Vehicle(UV).Since UVs operate under two different kinds of working conditions(i.e.surf...An algorithm based on the Boundary Element Method(BEM)is presented for designing the High Skew Propeller(HSP)used in an Underwater Vehicle(UV).Since UVs operate under two different kinds of working conditions(i.e.surface and submerged conditions),the design of such a propeller is an unwieldy task.This is mainly due to the fact that the resistance forces as well as the vessel efficiency under these conditions are significantly different.Therefore,some factors are necessary for the design of the opti-mum propeller to utilize the power under the mentioned conditions.The design objectives of the optimum propeller are to obtain the highest possible thrust and efficiency with the minimum torque.For the current UV,the main dimensions of the propeller are pre-dicted based on the given required thrust and the defined operating conditions.These dimensions(number of blades,pitch,diameter,expanded area ratio,thickness and camber)are determined through iterative procedure.Because the propeller operates at the stern of the UV where the inflow velocity to the propeller is non-uniform,a 5-blade HSP is preferred for running the UV.Finally,the propel-ler is designed based on the numerical calculations to acquire the improved hydrodynamic efficiency.展开更多
During the 2006 experiment campaign of HL-2A, about 2000 shots have been implemented, the plasma current of 433 kA and 3.0 s duration have been obtained. The wall conditioning methods such as glow discharge cleating ...During the 2006 experiment campaign of HL-2A, about 2000 shots have been implemented, the plasma current of 433 kA and 3.0 s duration have been obtained. The wall conditioning methods such as glow discharge cleating (GDC) , siliconization and Ti sublimation were applied. The experiment results of wall conditioning research will be introduced in this report. At the same time, the conceptual design of modification of HL-2A tokamak has obtained great progress, two modification schemes have been obtained, and the conceptual design results will be presented.展开更多
Air conditioning design has become an interesting aspect of human life with the aim of seeking environmental comfort for indoor spaces. Analytical work involves in the design of air-conditioning system is often time c...Air conditioning design has become an interesting aspect of human life with the aim of seeking environmental comfort for indoor spaces. Analytical work involves in the design of air-conditioning system is often time consuming and laborious. This work therefore aims at developing a visual interactive computer package for the design of air conditioning system using the international conference centre, University of Ibadan, Nigeria as a case study. Procedures for calculating heat gain through various building components were discussed in detail. Cooling load analysis was carried out using standard cooling load equations obtained from the American Society of Heating, Refrigeration and Air conditioning Engineers (ASHRAE) handbook. A computer program was developed using java programming language to compute the total cooling load in the building. The result from the computer program was obtained within a very short period of time when compared with that of the manual one. The cooling load of the building obtained was 3,194,414 btu/hr;a visual interactive computer package for the design of air conditioning system has been developed and tested positively;hence can be used to design air conditioning system for any building.展开更多
Two building factors-a longer thermal lag of more than one hour for building envelops and a lag of indoor radiation to convert into cooling load-have impact on the instantaneous heat input and instantaneous cooling lo...Two building factors-a longer thermal lag of more than one hour for building envelops and a lag of indoor radiation to convert into cooling load-have impact on the instantaneous heat input and instantaneous cooling load.So the two factors should be taken into account when selecting the weather parameters for air-conditioning system design.This paper developed a new statistic method for the rational selection of coincident solar irradiance,dry-bulb and wet-bulb temperatures.The method was applied to historic weather records of 25 years in Hong Kong to generate coincident design weather data.And the results show that traditional design solar irradiance,dry-bulb and wet-bulb temperatures may be significantly overestimated in many conditions,and the design weather data for the three different constructions is not kept constant.展开更多
In this paper, the author gives a brief introduction to the evolution of FIDIC conditions of contract, particularly the background of the 1999 new editions. A detailed discussion is made of the applying prospects refe...In this paper, the author gives a brief introduction to the evolution of FIDIC conditions of contract, particularly the background of the 1999 new editions. A detailed discussion is made of the applying prospects referring to the new editions of FIDIC Conditions of Contract in China in 6 aspects: revisions of the existing model contract forms; finding solutions to the payment delay problem; advocating the Design/Build and EPC turnkey project delivery system; clarifying the roles of supervising engineers and opening up the new regions of their work. This paper also prepares a short form model contract for small projects in China, improving the settlement of construction disputes.展开更多
By combining the practices of deep mine mining in Changguang Mine field and using the Universal Distinct Element Code 3 0(UDEC3 0) numerical computing method, the distribution characteristics of deformation field and ...By combining the practices of deep mine mining in Changguang Mine field and using the Universal Distinct Element Code 3 0(UDEC3 0) numerical computing method, the distribution characteristics of deformation field and stress field as well as the surrounding rock deformation regularity of soft rock roadway are analyzed under extremely complicated geological conditions, a technical principle of bolting to control the surrounding rock of roadway is put forward. And also using a dynamic control for surrounding rocks designing method, the supporting parameters and implement plan are rationally determined. The experimental tests have obtained a good controlling result of surrounding rock.展开更多
Actually recent investigation in developing semiconducting-superconducting composites based in CdS and Bi-based superconductors has attracted interest in processing thin superconducting films. In this work are reporte...Actually recent investigation in developing semiconducting-superconducting composites based in CdS and Bi-based superconductors has attracted interest in processing thin superconducting films. In this work are reported Bi-Pb-Sr-Ca-Cu-O (BPSCCO) thin films grown on MgO substrates by spray pyrolysis technique from a solution containing Bi(NO3)3, Pb(NO3)2, Sr(NO3)2, Ca(NO3)2 and Cu(NO3)2, with a subsequent solid state reaction for growing the Bi-based superconducting phases. Annealed films were characterized by X-ray diffraction, atomic absorption spectroscopy and resistance measurements. Interdependence between Pb content, annealing time and temperature, in the formation of superconducting phases was studied applying a fractional factorial design 3III4-2. Interrelation between Pb content, ta and Ta exists. The presence of Pb is necessary to stabilize the high-Tc phase but its content depends on the annealing conditions.展开更多
The high-speed EMUs for Jakarta-Bandung high-speed railway(HSR)represent an important image and symbol of the Jakarta-Bandung HSR Project.By relying on the technical platform of the model CR400 EMU,the EMU is upgraded...The high-speed EMUs for Jakarta-Bandung high-speed railway(HSR)represent an important image and symbol of the Jakarta-Bandung HSR Project.By relying on the technical platform of the model CR400 EMU,the EMU is upgraded for the customized design in terms of adaptability to geographical environment and track conditions,integration of Indonesian cultural characteristics,etc.In the course of development,in order to accurately grasp the boundary conditions and solve matching adaptation problems,studies are conducted on the exploration of design boundary and the related special technology.With the systematic analysis and design experience summary of the high-speed EMUs for Jakarta-Bandung HSR,the paper aims to provide reference for the O&M of high-speed EMUs for Jakarta-Bandung HSR and the other similar overseas HSR projects.展开更多
Recently,deep learning has been used to establish the nonlinear and nonintuitive mapping between physical structures and electromagnetic responses of meta-atoms for higher computational efficiency.However,to obtain su...Recently,deep learning has been used to establish the nonlinear and nonintuitive mapping between physical structures and electromagnetic responses of meta-atoms for higher computational efficiency.However,to obtain sufficiently accurate predictions,the conventional deep-learning-based method consumes excessive time to collect the data set,thus hindering its wide application in this interdisciplinary field.We introduce a spectral transfer-learning-based metasurface design method to achieve excellent performance on a small data set with only 1000 samples in the target waveband by utilizing open-source data from another spectral range.We demonstrate three transfer strategies and experimentally quantify their performance,among which the“frozen-none”robustly improves the prediction accuracy by∼26%compared to direct learning.We propose to use a complex-valued deep neural network during the training process to further improve the spectral predicting precision by∼30%compared to its real-valued counterparts.We design several typical teraherz metadevices by employing a hybrid inverse model consolidating this trained target network and a global optimization algorithm.The simulated results successfully validate the capability of our approach.Our work provides a universal methodology for efficient and accurate metasurface design in arbitrary wavebands,which will pave the way toward the automated and mass production of metasurfaces.展开更多
基金supported by the National Natural Science Foundation of China(Grant Nos.41176074,51209048,51379043,and 51409063)the High Technology Ship Scientific Research Project of Ministry of Industry and Information Technology of China(Grant No.G014613002)
文摘Different operating conditions (e.g. design and off-design) may lead to a significant difference in the hydrodynamics performance of a ship, especially in the total resistance and wake field of ships. This work investigated the hydrodynamic performance of the well-known KRISO 3600 TEU Container Ship (KCS) under three different operating conditions by means of Particle Image Velocimetry (P/V) and Computational Fluid Dynamics (CFD). The comparison results show that the use of PIV to measure a ship's nominal wake field is an important method which has the advantages of being contactless and highly accurate. Acceptable agreements between the results obtained by the two different methods are achieved. Results indicate that the total resistances of the KCS model under two off-design conditions are 23.88% and 13.92% larger than that under the designed condition, respectively.
基金Foundation item: Supported by the National Natural Science Foundation of China (Grant Nos. 41176074, 51379043 and 51409063)Acknowledgement This project was supported by the National Natural Science Foundation of China (Grant Nos. 41176074,51379043 and 51409063) and was conducted in response to the great support received from a basic research project entitled "Multihull Ship Technology Key Laboratory of Fundamental Science for National Defence", which was conducted at Harbin Engineering University. The authors would like to extend their sincere gratitude to their colleagues in the towing tank laboratory.
文摘Experimental tests were conducted to evaluate the hydrodynamic performance of an L-type podded propulsor in straight-ahead motion and off-design conditions using an open-water measuring instrument developed by the authors for podded propulsors, a ship model towing tank, and under water particle image velocimetry (PIV) measurement systems. Under the three types of conditions, the main parameters of an L-type podded propulsor were measured, including the propeller thrust and torque, as well as the thrust, side force, and moment of the whole pod unit.In addition, the flow field on the section between the propeller and the strut was analyzed. Experimental results demonstrate that the dynamic azimuthing rate and direction and the turning direction affect the forces on the propeller and the whole pod unit. Forces are asymmetrically distributed between the left and right azimuthing directions because of the effect of propeller rotation. The findings of this study provide a foundation for further research on L-type podded propulsors.
基金financially supported by the National Natural Science Foundation(40930847,31070442)the Natural Science Foundation of Fujian Province(2012J01150)Public science and technology research funds projects of ocean(201305016,201305041,201305022) and MELRI1003
文摘Harmful algal blooms(HABs) have led to extensive ecological and environmental issues and huge economic losses.Various HAB control techniques have been developed,and biological methods have been paid more attention.Algicidal bacteria is a general designation for bacteria which inhibit algal growth in a direct or indirect manner,and kill or damage the algal cells.A metabolite which is strongly toxic to the dinoflagellate Alexandrium tamarense was produced by strain DH46 of the alga-lysing bacterium Alteromonas sp.The culture conditions were optimized using a single-factor test method.Factors including carbon source,nitrogen source,temperature,initial pH value,rotational speed and salinity were studied.The results showed that the cultivation of the bacteria at 28℃ and 180 r min-1with initial pH 7 and 30 salt contcentration favored both the cell growth and the lysing effect of strain DH46.The optimal medium composition for strain DH46 was determined by means of uniform design experimentation,and the most important components influencing the cell density were tryptone,yeast extract,soluble starch,NaNO3 and MgSO4.When the following culture medium was used(tryptone 14.0g,yeast extract 1.63g,soluble starch 5.0 g,NaNO3 1.6 g,MgSO4 2.3 g in 1L),the largest bacterial dry weight(7.36 g L-1) was obtained,which was an enhancement of 107% compared to the initial medium;and the algal lysis rate was as high as 98.4% which increased nearly 10% after optimization.
文摘Public vehicle seats are used under diverse conditions, therefore, the design of these seats is desired to be universal design. In the past study, seat swing function used in public vehicle seats, and it was optimized from the mechanical aspect under diverse conditions. Seat swing function is the function to prevent hip-sliding force, which causes sitting uncomfortable feeling. Recent study clarified that the reduction of blood flow in the legs causes physiological dysfunction, which is one of the causes of Long Flight Thrombosis, and the social issue. Thus, in the design of seat swing function, it is necessary to consider not only mechanical aspects but also physiological aspects. For the universal design of seat swing function considering the physiological aspect (blood flow), the influence of blood flow on seat swing function in diverse conditions was analyzed. The method of this study was measuring blood flow and body pressure distribution on seat cushion of seat swing function under diverse conditions. From the analysis, it was indicated that it was necessary to reduce the compressive force behind the knees of the user conditions; the short physique and the high weight physique in standard sitting posture, for the universal design of seat swing function to prevent reduction of blood flow in the legs.
基金Supported by National Basic Research Program of China(973 Pro-gram)(2003CB71600)~~
文摘[ Objective] This study was to improve the biomass of Navicula tenera, and thus to provide reference for achieving the industrial production of Navicula tenera. [Method] The feasibility of using sewage to cultivate Navicula tenera was preliminarily investigated based on the consideration of regional characteristics; and a series of culture conditions including the nutrient source of nitrogen(N), phosphorus(P), iron(Fe), silicon( Si ) and the salinity in medium for culturing Navicula tenera, were optimized by single factor test and orthogonal design.[Result] The optimized conditions for cultivating Navicula tenera using sewage are as follows: the water from Xiaoerlou Artificial Lake of Nanjing University of Technology as basic solvent; 360 mg/L urea; 150 mg/L N2HPO4 · 12H2O; 50 mg/L ferric citrate; 2 000 mg/L Na2SiO3 · 9H2O; 2.0 mol/L salinity. Navicula tenera was strongly adaptive to sewage and could well uptake the nutrient sources in the sewage. Under the optimized conditions, the culture cost decreased, and meanwhile the biomass of Navicula tenera reached 4. 766 g/L which is 3.57 multiples over original medium and 1.9 multiples over optimized medium No. 1.[ Conclusion] This study laid a foundation for the combination of culturing Navicula tenera in large scale and sewage treatment.
基金the Shenhuo Mining Group Co.Ltd.,China for its financial support.At the same time,we also thank the National Natural Science Foundation of China(No.40373044)the Natural Science Foundation of Jiangsu Province (No.05KJD610209) for their supportthe Jiangsu Key Laboratory of Resources and Environmental Information Engineering for its technical support.
文摘We studied how bioflocculants,produced by white-rot fungi,affect flocculation in slime water.Based on a test in an orthogonal design,flocculation conditions were optimized.The results show that flocculation activity is at its highest when the following conditions are met:slime water concentration 27.42 g/L;coagulant aid(CaCl_2) mass concentration 5.0 g/L;two-segment stirrings:the first at a stirring speed of 60 r/min for 180 s and the second 180 r/min for 60 s;a pH of 11 and a flocculant concentration of 15 mL/L.The flocculation activity can be up to 98.71%of bioflocculants at the time.Further experiments indicate that most of the flocculation active material is found outside the mycelium cells.This is the extracellular secretion produced by mycelium cells during the fermentation process.This flocculant has strong thermal stability.Many kinds of cations have a flocculation function to assist bioflocculants.This aid-flocculation effect of the divalent cation Ca^(2+) is obvious in the bioflocculant produced by the white-rot fungus.Therefore,this is of great value when applied to control engineering in the battle against water pollution.
基金Supported by Science and Technology Project of Shenzhen City (JC200903180710A)~~
文摘[Objective] This study aimed to increase the expression level of immun- odominant membrane protein gene (Imp) of phytoplasmas in E. coil BL21 (DE3). [Method] On the basis of orthogonal experiment, effects of different culture conditions on recombinant bacteria E. coil BL21-pET-28a(+)-Imp were investigated. Based on the obtained optimal culture condition, effects of different induction conditions on the ex- pression level of Imp protein were explored. The expression level of Imp fusion pro- tein was analyzed by using SDS-PAGE and Gene Tools software. [Result] The re- sults showed that the optimal conditions for culture were at 37℃, pH 7.0, with liq- uid volume of 20% and oscillation speed of 200 r/min, for induction were at 37℃ for 6 h, with initial OD600 of about 1.5 and IPTG final concentration of 0.1 mmol/L. [Conclusion] The expression level of Imp achieved 70.98 mg/L under the optimal conditions. Optimized conditions for expression of Imp fusion protein in E. coil were determined.
文摘Sequential methodology based on the application of three types of experimental designs was used to optimize the fermentation conditions for elastase production from mutant strain ZJUEL31410 of Bacillus licheniformis in shaking flask cul- tures. The optimal cultivation conditions stimulating the maximal elastase production consist of 220 r/min shaking speed, 25 h fermentation time, 5% (v/v) inoculums volume, 25 ml medium volume in 250 ml Erlenmeyer flask and 18 h seed age. Under the optimized conditions, the predicted maximal elastase activity was 495 U/ml. The application of response surface methodology resulted in a significant enhancement in elastase production. The effects of other factors such as elastin and the growth factor (corn steep flour) on elastase production and cell growth were also investigated in the current study. The elastin had no significant effect on enzyme-improved production. It is still not clear whether the elastin plays a role as a nitrogen source or not. Corn steep flour was verified to be the best and required factor for elastase production and cell growth by Bacillus licheniformis ZJUEL31410.
基金supported by the marine research center of Amirkabir University of Technology
文摘An algorithm based on the Boundary Element Method(BEM)is presented for designing the High Skew Propeller(HSP)used in an Underwater Vehicle(UV).Since UVs operate under two different kinds of working conditions(i.e.surface and submerged conditions),the design of such a propeller is an unwieldy task.This is mainly due to the fact that the resistance forces as well as the vessel efficiency under these conditions are significantly different.Therefore,some factors are necessary for the design of the opti-mum propeller to utilize the power under the mentioned conditions.The design objectives of the optimum propeller are to obtain the highest possible thrust and efficiency with the minimum torque.For the current UV,the main dimensions of the propeller are pre-dicted based on the given required thrust and the defined operating conditions.These dimensions(number of blades,pitch,diameter,expanded area ratio,thickness and camber)are determined through iterative procedure.Because the propeller operates at the stern of the UV where the inflow velocity to the propeller is non-uniform,a 5-blade HSP is preferred for running the UV.Finally,the propel-ler is designed based on the numerical calculations to acquire the improved hydrodynamic efficiency.
文摘During the 2006 experiment campaign of HL-2A, about 2000 shots have been implemented, the plasma current of 433 kA and 3.0 s duration have been obtained. The wall conditioning methods such as glow discharge cleating (GDC) , siliconization and Ti sublimation were applied. The experiment results of wall conditioning research will be introduced in this report. At the same time, the conceptual design of modification of HL-2A tokamak has obtained great progress, two modification schemes have been obtained, and the conceptual design results will be presented.
文摘Air conditioning design has become an interesting aspect of human life with the aim of seeking environmental comfort for indoor spaces. Analytical work involves in the design of air-conditioning system is often time consuming and laborious. This work therefore aims at developing a visual interactive computer package for the design of air conditioning system using the international conference centre, University of Ibadan, Nigeria as a case study. Procedures for calculating heat gain through various building components were discussed in detail. Cooling load analysis was carried out using standard cooling load equations obtained from the American Society of Heating, Refrigeration and Air conditioning Engineers (ASHRAE) handbook. A computer program was developed using java programming language to compute the total cooling load in the building. The result from the computer program was obtained within a very short period of time when compared with that of the manual one. The cooling load of the building obtained was 3,194,414 btu/hr;a visual interactive computer package for the design of air conditioning system has been developed and tested positively;hence can be used to design air conditioning system for any building.
文摘Two building factors-a longer thermal lag of more than one hour for building envelops and a lag of indoor radiation to convert into cooling load-have impact on the instantaneous heat input and instantaneous cooling load.So the two factors should be taken into account when selecting the weather parameters for air-conditioning system design.This paper developed a new statistic method for the rational selection of coincident solar irradiance,dry-bulb and wet-bulb temperatures.The method was applied to historic weather records of 25 years in Hong Kong to generate coincident design weather data.And the results show that traditional design solar irradiance,dry-bulb and wet-bulb temperatures may be significantly overestimated in many conditions,and the design weather data for the three different constructions is not kept constant.
文摘In this paper, the author gives a brief introduction to the evolution of FIDIC conditions of contract, particularly the background of the 1999 new editions. A detailed discussion is made of the applying prospects referring to the new editions of FIDIC Conditions of Contract in China in 6 aspects: revisions of the existing model contract forms; finding solutions to the payment delay problem; advocating the Design/Build and EPC turnkey project delivery system; clarifying the roles of supervising engineers and opening up the new regions of their work. This paper also prepares a short form model contract for small projects in China, improving the settlement of construction disputes.
文摘By combining the practices of deep mine mining in Changguang Mine field and using the Universal Distinct Element Code 3 0(UDEC3 0) numerical computing method, the distribution characteristics of deformation field and stress field as well as the surrounding rock deformation regularity of soft rock roadway are analyzed under extremely complicated geological conditions, a technical principle of bolting to control the surrounding rock of roadway is put forward. And also using a dynamic control for surrounding rocks designing method, the supporting parameters and implement plan are rationally determined. The experimental tests have obtained a good controlling result of surrounding rock.
文摘Actually recent investigation in developing semiconducting-superconducting composites based in CdS and Bi-based superconductors has attracted interest in processing thin superconducting films. In this work are reported Bi-Pb-Sr-Ca-Cu-O (BPSCCO) thin films grown on MgO substrates by spray pyrolysis technique from a solution containing Bi(NO3)3, Pb(NO3)2, Sr(NO3)2, Ca(NO3)2 and Cu(NO3)2, with a subsequent solid state reaction for growing the Bi-based superconducting phases. Annealed films were characterized by X-ray diffraction, atomic absorption spectroscopy and resistance measurements. Interdependence between Pb content, annealing time and temperature, in the formation of superconducting phases was studied applying a fractional factorial design 3III4-2. Interrelation between Pb content, ta and Ta exists. The presence of Pb is necessary to stabilize the high-Tc phase but its content depends on the annealing conditions.
文摘The high-speed EMUs for Jakarta-Bandung high-speed railway(HSR)represent an important image and symbol of the Jakarta-Bandung HSR Project.By relying on the technical platform of the model CR400 EMU,the EMU is upgraded for the customized design in terms of adaptability to geographical environment and track conditions,integration of Indonesian cultural characteristics,etc.In the course of development,in order to accurately grasp the boundary conditions and solve matching adaptation problems,studies are conducted on the exploration of design boundary and the related special technology.With the systematic analysis and design experience summary of the high-speed EMUs for Jakarta-Bandung HSR,the paper aims to provide reference for the O&M of high-speed EMUs for Jakarta-Bandung HSR and the other similar overseas HSR projects.
基金support from the National Natural Science Foundation of China (Grant Nos.62027820,61975143,61735012,and 62205380).
文摘Recently,deep learning has been used to establish the nonlinear and nonintuitive mapping between physical structures and electromagnetic responses of meta-atoms for higher computational efficiency.However,to obtain sufficiently accurate predictions,the conventional deep-learning-based method consumes excessive time to collect the data set,thus hindering its wide application in this interdisciplinary field.We introduce a spectral transfer-learning-based metasurface design method to achieve excellent performance on a small data set with only 1000 samples in the target waveband by utilizing open-source data from another spectral range.We demonstrate three transfer strategies and experimentally quantify their performance,among which the“frozen-none”robustly improves the prediction accuracy by∼26%compared to direct learning.We propose to use a complex-valued deep neural network during the training process to further improve the spectral predicting precision by∼30%compared to its real-valued counterparts.We design several typical teraherz metadevices by employing a hybrid inverse model consolidating this trained target network and a global optimization algorithm.The simulated results successfully validate the capability of our approach.Our work provides a universal methodology for efficient and accurate metasurface design in arbitrary wavebands,which will pave the way toward the automated and mass production of metasurfaces.