The neutrophil–osteogenic cell axis promotes bone destruction in periodontitis

The immune-stromal cell interactions play a key role in health and diseases. In periodontitis, the most prevalent infectious disease in humans, immune cells accumulate in the oral mucosa and promote bone destruction by inducing receptor activator of nuclear factor-κB ligand (RANKL) expression in osteogenic cells such as osteoblasts and periodontal ligament cells. However, the detailed mechanism underlying immune–bone cell interactions in periodontitis is not fully understood. Here, we performed single-cell RNAsequencing analysis on mouse periodontal lesions and showed that neutrophil–osteogenic cell crosstalk is involved in periodontitis-induced bone loss. The periodontal lesions displayed marked infiltration of neutrophils, and in silico analyses suggested that the neutrophils interacted with osteogenic cells through cytokine production. Among the cytokines expressed in the periodontal neutrophils, oncostatin M (OSM) potently induced RANKL expression in the primary osteoblasts, and deletion of the OSM receptor in osteogenic cells significantly ameliorated periodontitis-induced bone loss. Epigenomic data analyses identified the OSM-regulated RANKL enhancer region in osteogenic cells, and mice lacking this enhancer showed decreased periodontal bone loss while maintaining physiological bone metabolism. These findings shed light on the role of neutrophils in bone regulation during bacterial infection, highlighting the novel mechanism underlying osteoimmune crosstalk.

Triaxial mechanical properties and precursors of instability damage in rocks with different laminar dip angles

To investigate the mechanical failure characteristics of volcanic breccia with different bedding dip angles in deep layers,triaxial compression experiments were conducted on specimens taken from volcanic rock layers in the Junggar Basin,Xinjiang.By changing the confining pressure,we compared the mechanical properties,acoustic emission characteristics,and fractal features of the acoustic emission sequence for volcanic breccia with different bedding dip angles.The research results indicate that as the bedding dip angle increases,peak strength,internal friction angle,and cohesion exhibit a“U-shaped”variation pattern.The correlation dimension of the acoustic emission amplitude was calculated using the G-P algorithm,revealing that volcanic breccia exhibits fractal characteristics.Furthermore,a positive correlation between the correlation dimension and the bedding dip angle was observed.When the stress of the test specimen reaches 0.8σ_(c),there is a significant decrease in the fractal dimension,serving as a criterion for predicting the failure of the specimen.By analyzing the changes in fractal dimension at different stress levels,the slope of the fractal dimension△D<-0.5 can be used as a precursor to failure for test specimens with a bedding dip angle of 15°≤β≤45°.Similarly,when the bedding dip angle of the specimen is 0°≤β<15°or 45°<β≤90°,△D<0 can be considered as a precursor to specimen failure.This study provides theoretical references for understanding the failure patterns of volcanic rock.

Strength and damage evolution mechanism of rock mass with holes under cyclic loading

The damage and failure law of rock mass with holes is of great significance to the stability control of roadways. This study investigates the mechanical properties and failure modes of porous rock masses under cyclic loading, elucidates the acoustic emission (AE) characteristics and their spatial evolution, and establishes the interrelation among AE, stress, strain, time, and cumulative damage. The results reveal that the rock mass with holes and the intact rock mass show softening and hardening characteristics after cyclic loading. The plastic strain of the rock mass with holes is smaller than that of the intact rock mass, and the stress −strain curve shows hysteresis characteristics. Under uniaxial compression, the pore-bearing rock mass shows the characteristics of higher ringing count, AE energy, b-value peak, and more cumulative ringing count in the failure stage, while it shows lower characteristics under cyclic action. At the initial stage of loading, compared with the intact rock mass, the pore-containing rock mass shows the characteristics of a low b-value. The AE positioning and cumulative damage percentage are larger, and the AE positioning is denser around the hole. The specimen with holes is mainly shear failure, and the complete specimen is mainly tensile shear failure.

Macro-micro behaviors and failure mechanism of frozen weakly cemented mudstone

Understanding the mechanical properties and multiscale failure mechanism of frozen soft rock is an important prerequisite for the construction safety of tunnels,artificially frozen ground and other infrastructure in cold regions.In this study,the triaxial compression test are performed on mudstone in the weakly cemented soft rock strata in the mining area of western China,and the mechanical characteristics and failure mechanism of weakly cemented mudstone are systematically investigated under the combined action of freezing and loading.Furthermore,the quantitative relationship between the microstructural parameters and the macroscopic strength and deformation parameters is established based on fractal theory.Thus,the failure mechanism of frozen weakly cemented mudstone is revealed on both micro- and macro-scales.The results show that temperature and confining pressure significantly affects the elastic modulus and peak strength of weakly cemented mudstone.With decreasing temperature,the compressive strength increases,while the corresponding peak strain decreases gradually.On the deformation curve,the plastic deformation stage is shortened,and the brittle fracture feature at the post-peak stage is more prominent,and the elastic modulus correspondingly increases with decreasing temperature.Under low-temperature conditions,most of the weakly cemented mudstone undergoes microscopic shear failure along the main fracture surface.The micro-fracture morphology characteristics of weakly cemented mudstone under different temperatures are quantified via the fractal dimension,and an approximately exponential relationship can be obtained among the fractal dimension and the temperature,compressive strength and elastic modulus.

Airblast evolution initiated by Wangjiayan landslides in the M_(s)8.0 Wenchuan earthquake and its destructive capacity analysis

Airblasts,as one common phenomenon accompanied by rapid movements of landslides or rock/snow avalanches,commonly result in catastrophic damages and are attracting more and more scientific attention.To quantitatively analyze the intensity of airblast initiated by landslides,the Wangjiayan landslide,occurred in the Wenchuan earthquake,is selected here with the landslide propagation and airblast evolution being studied using FLUENT by introducing the Voellmy rheological law.The results reveal that:(1)For the Wangjiayan landslide,its whole travelling duration is only 12 s with its maximum velocity reaching 36 m/s at t=10 s;(2)corresponding to the landslide propagation,the maximum velocity,28 m/s,of the airblast initiated by the landslide also appears at t=10 s with its maximum pressure reaching594.8 Pa,which is equivalent to violent storm;(3)under the attack of airblast,the load suffered by buildings in the airblast zone increases to 1300 Pa at t=9.4 s and sharply decreased to-7000 Pa as the rapid decrease of the velocity of the sliding mass at t=10 s,which is seriously unfavorable for buildings and might be the key reason for the destructive collapse of buildings in the airblast zone of the Wangjiayan landslide.

Characteristics and main controlling factors of helium resources in the main petroliferous basins of the North China Craton

At present, the main controlling factors of helium accumulation is one of the key scientific problems restricting the exploration and development of helium reservoir. In this paper, based on the calculation results of He generation rate and the geochemical characteristics of the produced gas, both the similarities and differences between natural gas and He resources in the Bohai Bay, Ordos and the surrounding Songliao Basin are compared and analyzed, discussing the main controlling factors of helium resources in the three main petroliferous basins of the North China Craton. It is found that the three basins of Bohai Bay, Ordos and Songliao have similar characteristics of source rocks, reservoirs and cap rocks, that's why their methane resource characteristics are essentially the same. The calculated ~4He generation per cubic metamorphic crystalline basement in the three basins is roughly equivalent, which is consistent with the measured He resources, and it is believed that the ~4He of radiogenic from the crust is the main factor controlling the overall He accumulation in the three basins;there is almost no contribution of the mantle-derived CH_4, which suggests that the transport and uplift of mantle-derived ~3He carried by the present-day magmatic activities along the deep-large faults is not the main reason for the mantle-derived ~3He mixing in the basins. Combined with the results of regional volcanic and geophysical studies,it is concluded that under the background of the destruction of North China Craton, magma intrusion carried a large amount of mantle-derived material and formed basic volcanic rocks in the Bohai Bay Basin and Songliao Basin, which replenished mantle-derived ~3He for the interior of the basins, and that strong seismic activities in and around the basins also promoted the upward migration of mantle source ~3He. This study suggests that the tectonic zone with dense volcanic rocks in the Cenozoic era and a high incidence of historical strong earthquakes history may be a potential area for helium resource exploration.

Bone destruction of orbital wall in idiopathic orbital inflammatory pseudotumor:does it always imply malignancy?

AIM:To assess the clinical presentations and outcomes of idiopathic orbital inflammatory pseudotumor(IOIP)patients with orbital wall bone destruction(OWBD)and to propose an expanded classification system that includes bone destruction.METHODS:The study retrospectively reviewed clinical presentations,imaging findings,treatment modalities,and outcomes of six patients diagnosed histopathologically with IOIP and OWBD at the Beijing Tongren Hospital,Capital Medical University between October 2018 and June 2021.RESULTS:Over two years,6(10%)of 60 IOIP patients at our hospital exhibited OWBD,but this may overrepresent severe cases.The cohort consisted of three men and three women,aged 17 to 60y(mean 35.5±16.1y).Presenting symptoms included proptosis,eyelid swelling,decreased visual acuity with pain,and palpable mass.Imaging revealed multiple anatomical structures involved with the medial wall being the most common site of bone destruction.Histopathological examination showed classic type in five patients and sclerosing type in one patient.All patients underwent surgical resection followed by methylprednisolone treatment.Follow-up(mean 30.3±3.1mo)indicated three patients had no recurrence,while others had varying degrees of symptom persistence or recurrence.CONCLUSION:IOIP with bone destruction is a rare but significant subtype that mimics malignancy,leading to potential diagnostic and therapeutic challenges.Our findings suggest that complete surgical resection combined with adjunctive glucocorticoid therapy can yield favorable outcomes.However,larger-scale studies are needed to further optimize therapeutic approaches.

The Al_(2)O_(3)and Mn/Al_(2)O_(3)sorbents highly utilized in destructive sorption of NF_(3)

NF_(3)is commonly used as an etching and cleaning gas in semiconductor industry,however it is a strongly greenhouse gas.Therefore,the destruction of disposal NF_(3)is an urgent task to migrate the greenhouse effect.Among the technologies for NF_(3)abatement,the destructive sorption of NF_(3)over metal oxides sorbents is an effective way.Thus,the search for a highly reactive and utilized sorbent for NF_(3)destruction is in great demand.In this work,AlOOH supported on carbon-sphere(AlOOH/CS)as precursors were synthesized hydrothermally and heat-treated to prepare the Al_(2)O_(3)sorbents.The influence of AlOOH/CS hydrothermal temperatures on the reactivity of derived Al_(2)O_(3)sorbents for NF_(3)destruction was investigated,and it is shown that the Al2O3 from AlOOH/CS hydro-thermalized at 120℃is superior to others.Subsequently,the optimized Al_(2)O_(3)was covered by Mn(OH)x to prepare Mn/Al_(2)O_(3)sorbents via changing hydrothermal temperatures and Mn loadings.The results show that the Mn/Al_(2)O_(3)sorbents are more utilized than bare Al_(2)O_(3)in NF_(3)destructive sorption due to the promotional effect of Mn_(2)O_(3)as surface layer on the fluorination of Al_(2)O_(3)as substrate,especially the optimal 5%Mn/Al2O3(160℃)exhibits a utilization percentage as high as 90.4%,and remarkably exceeds all the sorbents reported so far.These findings are beneficial to develop more efficient sorbents for the destruction of NF_(3).

Preclinical Verification of Modulated Electro-Hyperthermia—Part II. In Vivo Research

The treatments of malignant diseases nowadays are rapidly developing. One of the groups of novel therapies applies electromagnetic fields to destroy the malignant lesions. The thermal (heating) and nonthermal (polarization, molecular excitations) processes are combined in novel methods. The non-ionizing energy absorption from the electric field may produce substantial heat, increasing the targeted lesion’s temperature and inducing hyperthermic effects. The modulated electro-hyperthermia (mEHT) uses thermal conditions to optimize and accelerate the chemical reactions induced by the nonthermal excitation of the electric field. The mEHT cooperates with the body’s homeostatic control and harmonizes the mutual efforts to destroy the malignancy. Our objective is to show in vivo proof of the combined complementary electromagnetic impact on various tumors produced by mEHT. Furthermore, we present evidence of the increasing efficacy of the complementary application of mEHT with conventional treatments.

Life-Cycle Bearing Capacity for Pre-Stressed T-beams Based on Full-Scale Destructive Test

To investigate the evolution of load-bearing characteristics of pre-stressed beams throughout their service life and to provide a basis for accurately assessing the actual working state of damaged pre-stressed concrete T-beams,destructive tests were conducted on full-scale pre-stressed concrete beams.Based on the measurement and ana-lysis of beam deflection,strain,and crack development under various loading levels during the research tests,combined with the verification coefficient indicators specified in the codes,the verification coefficients of bridges at different stages of damage can be examined.The results indicate that the T-beams experience complete,incom-plete linear,and non-linear stages during the destructive test process.In the complete linear elastic stage,both the deflection and bottom strain verification coefficients comply with the specifications,indicating a good structural load-bearing capacity no longer adheres to the code’s requirements.In the non-linear stage,both coefficients exhi-bit a sharp increase,resulting in a further decrease in the structure’s load-bearing capacity.According to the pro-visions of the current code,the beam can be in the incomplete linear stage when both values fall within the code’s specified range.The strain verification coefficient sourced from the compression zone at the bottom of theflange is not recommended for assessing the bridge’s load-bearing capacity.

Gold mineralization in Jiaodong Peninsula and destruction of North China Craton:Insights from Mesozoic granite

Large-scale tectonic magmatism and extensive gold mineralization have occurred in the eastern North China Craton(NCC)(Jiaodong and Liaodong peninsulas)since Mesozoic,which indicated that the region experienced decratonization process.The genesis type of granites evolved from S-type to I-type and A-type during Late Jurassic to Early Cretaceous,and thus reflects the evolution of geodynamics in the Late Mesozoic,indicating the varied subduction process of the Paleo-Pacific Plate(PPP)and the craton destruction.The evolution of geochemical features shows that the mantle beneath the Jiaodong Peninsula evolved from EM-II in Jurassic to EM-I in Early Cretaceous,demonstrating that the mantle switched from enriched to depleted.The gold of decratonic gold deposits in Jiaodong Peninsula mainly comes from the lithospheric mantle domains metasomatized by fluids derived from the metamorphism and dehydration of the subducted slab in the mantle transition zone.The rapid decomposition of minerals leads to the concentrated release of ore-forming fluids,and this process leads to the explosive gold mineralization during the craton destruction.Extensive magmatic uplift and extensional structures,triggered by the craton destruction in Early Cretaceous formed the extensional tectonic system,providing space for the decratonic gold deposits in Jiaodong Peninsula.

Ethylene Copolymerization with Linear and End-Cyclized Olefins via a Metallocene Catalyst:Polymerization Behavior and Thermal Properties of Copolymers

Olefin solution polymerization can be used to obtain high-performance polyolefin materials that cannot be obtained via other polymerization processes.Polyolefin elastomers(POE)are a typical example.Due to cost,only a few linear a-olefins(e.g.,1-butene,1-hexene,and 1-octene)are used as comonomers in solution polymerization in industry.However,a-olefin comonomers with other structures may have different effects on polymerization in comparison with common linear ones.Moreover,the properties of the corresponding materials may differ significantly.In this work,copolymers of ethylene with linear and endcyclized a-olefins are synthesized using a metallocene catalyst.The copolymerization of ethylene with linear a-olefins results in a higher turn-over frequency(TOF)and lower incorporation than copolymerization with end-cyclized a-olefins,which may indicate that end-cyclized a-olefins have a higher coordination probability and lower insertion rate.In this reaction,the comonomer is distributed randomly in the polymer chain and efficiently destroys crystallization.End-cyclized a-olefins exhibit a much stronger crystallization destructive capacity(CDC)in the copolymer than linear a-olefins,possibly because linear a-olefins act mainly in the radial direction of the main chain of the polymer,while end-cyclized a-olefins act mainly in the axial direction of the main chain.

Influence of vacuoles with gas–liquid inclusions on the thermobaric destruction conditions of natural quartz under dynamic heating in an RF-TICP torch system

In the present work,the turbulent mixing process of a polydisperse quartz particle flow with a plasma stream generated by a radio-frequency(RF)inductively coupled plasma torch was numerically studied.The thermobaric stress in the quartz particles under dynamic heating in a heterogeneous plasma flow was determined by a two-stage approximation approach.The effect of the presence of vacuoles in natural quartz on the particle thermobaric destruction conditions was studied.It was found that the equivalent thermal and baric stresses in quartz particles may significantly increase in the presence of vacuoles within a small gas volume fraction.The influence of the regime and energetic working conditions of an RF inductively coupled plasma torch system on the particle thermobaric destruction conditions was examined,and a recommendation was given to promote the degree of thermobaric destruction of quartz particles,which is of substantial importance for improving the overall enrichment efficiency of quartz concentrates.

miR-483-5p regulates osteoclast generation by targeting Timp2

Objective:To investigate whether miR-483-5p regulates osteoclast generation by targeting Timp2.miR-483-5p can promote osteoclast differentiation and bone destruction.Methods:Target genes of miR-483-5p were predicted by miRNAs target gene prediction software TargetScan8.0,and wild type and mutant 3'UTR plasmids were constructed.Dual luciferase reporter genes were used to verify whether target genes had a targeted regulatory relationship with miR-483-5p.Western blotting was used to detect the corresponding changes in the expression level of target protein after adjusting the level of miR-483-5p in cells.Cells were transfected or infected with target gene siRNA or target protein lentivirus,and TRAP staining and q-PCR assays were performed.In addition,for osteoclast induction experiment,RAW264.7 cells were co-transfected with ago-miR-483-5p and target protein-overexpressed lentiviruses q-PCR and TRAP staining were performed respectively.Results:Bioinformatics software was used to predict the target gene of miR-483-5p,and the Timp2 gene was found to regulate osteoclasts,and the dual luciferase reporter detection system found that miR-483-5p could be associated with the 3-UTR of the predicted target gene Timp2 gene.There are complementary loci and targeted regulatory relationship between them.Subsequently,we upregulated miR-483-5p in RAW264.7 cells to reduce the expression of Timp2.Compared with the normal group,the number of osteoclasts and the expression of osteoclast-specific genes increased significantly after the induction of Timp2 in knockdown cells.After co-transfection of target gene and miR-483-5p into cells,the number of osteoclasts and the expression of specific genes decreased significantly compared with the normal group.Conclusion:Timp2 is a downstream target gene of miR-483-5p and is involved in and inhibits osteoclast generation.

Radio Direction Finding Method to Mitigate Tsunami Risk in Sierra Leone

In this study,the Radio Direction Finding method is proposed for the detection of electromagnetic signals,in the VLF band,to try to anticipate the occurrence of potentially destructive geophysical events.The experimentation concerns the interception of electromagnetic anomalies in Sierra Leone,in the five-day time window,associated with seismic events that could potentially generate tsunamis.The area of investigation is Sierra Leone,whose coastline is subjected to tidal wave hazards triggered by earthquakes generated in the Mid-Atlantic Ridge.Although Sierra Leone is not affected by recurrent earthquakes,there is nevertheless a low probability,estimated at 2 percent,of the occurrence of destructive earthquakes in the next 50 years.Also in estimates,the risk of rogue and potentially damaging waves is estimated to strike the Sierra Leone coast at least once in the next 10 years.The Radio Direction Finding experiment carried out continuously 24/7,has shown a close relationship between increased radio-anomalies,in the frequencies of 6,000 Hz,a time window between electromagnetic anomaly detection and the imminence of an earthquake,and higher frequency times for the risk of earthquake occurrence in the Mid-Atlantic Ridge.

Theoretical Study on Destruction Mechanism of 2,3,7,8-TCDD by O3 and NO3

In order to improve the destruction efficiency of dioxins and also for developing new dioxin control technology, the destruction mechanisms of 2,3,7,8-tetrachlorodihenzo-p-dioxin （2,3,7,8-TCDD） by O3 and NO3, were investigated employing quantum chemical calculations. For involved reactions, the microcosmic reaction processes were analyzed and depicted in detail based on geometry optimizations made by the B3LYP/6-31G（d） method. At the same time, the reaction activation energies were also calculated at the MP2/6- 311G（d,p）//B3LYP/6-31G（d） level. Configuration analysis indicated that 2,3,7,8-TCDD could be destroyed by 03 and NO3 in two different ways. The destruction of 2,3,7,8-TCDD by 03 proceeded via the addition of 03 and the cleavage of C=C while the destruction of 2,3,7,8-TCDD by NO3 proceeded via the substitution of chlorine by NO3. Calculated results show that, the activation energy of the destruction reaction of 2,3,7,8-TCDD by NO3 （267.48 kJ/mol） is much larger than that of the destruction reaction of 2,3,7,8-TCDD by O3 （51.20 kJ/mol）. This indicated that the destruction of 2,3,7,8-TCDD by 03 is much more efficient than that of 2,3,7,8-TCDD by NO3. The reason why the activation energy for the destruction reaction of 2,3,7,8-TCDD by NO3 is so large, is also discussed.

快速破坏性髋关节病2例

病例快速破坏性髋关节病(Rapidly destructive hipdisease,RDHD)是一种不常见、病因不明确、侵犯股骨头及髋臼、导致髋关节在短期内迅速破坏的的髋关节疾病。现将我院诊断的2例报告如下。例1,男,54岁。1997年7月在外院诊断为'左髋结核',

Flexural destructive process of unidirectional carbon/carbon composites reinforced with in situ grown carbon nanofibers

Unidirectional carbon/carbon（C/C） composites modified with in situ grown carbon nanofibers（CNFs） were prepared by catalysis chemical vapor deposition. The effect of in situ grown CNFs on the flexural properties of the C/C composites was investigated by detailed analyses of destructive process. The results show that there is a sharp increase in the flexural load-displacement curve in the axial direction of the CNF-C/C composites, followed by a serrated yielding phenomenon similar to the plastic materials. The failure mode of the C/C composites modified with in situ grown CNFs is changed from the pull-out of single fiber to the breaking of fiber bundles. The existence of interfacial layer composed by middle-textured pyrocarbon, CNFs and high-textured pyrocarbon can block the crack propagation and change the propagation direction of the main crack, which leads to the higher flexural strength and modulus of C/C composites.

Determination of Optimum Conditions for Cell-wall Destruction of Auricularia auricula Mycelia under the Synergistic Effect of Ultrasonic Waves and Repeated Freeze-thaw Cycles

We aimed to investigate the synergistic effects of ultrasonic waves and repeated freeze-thaw cycles on cel-wal destruction of Auricularia auric-ula mycelia, and determine the best combination of conditions for cel-wal destruc-tion of Auricularia auricula mycelia. [Method] The effects of destruction time, added water, destruction times, freeze time and number of freeze-thaw cycles during ultra-sonic treatment on polysaccharide yield were investigated by single-factor test in our research. The optimum conditions for cel-wal destruction of Auricularia auricula mycelia by the synergistic effect of ultrasonic waves and repeated freeze-thaw cycles were ascertained by orthogonal test. [Result] The results of single test indicated the best combination of conditions was as fol ows： freeze time, 30 min; destruction time, 20 min; added water, 15 times; destruction times, 2 times; number of freeze-thaw cycles, 3 cycles. The results of orthogonal test indicated the influencing factors ranked as destruction time 〉 destruction times 〉 freeze time. The best combination of conditions was as fol ows： freeze time, 30 min; destruction time, 25 min; destruc-tion times, 2 times. Under above conditions, the polysaccharide yield reached 57.76 mg/g. [Conclusion] This research would provide a basis and reference for practical production.

Study on the Ecological Construction in the Process of Urbanization——A Case Study of Ecological Protection Green Belt Design for Shanghai A20 Out Ring (Changning Section)

With the further development of urbanization,the conflicts between human beings and the nature has been gradually aggravated;it is urgent to establish balanced natural ecology in the process of urbanization,and harmony between human beings and environment.This study took the ecological landscape project of Shanghai A20 Out Ring (Changning Section) for an example to research and rethink about the ecological construction in the process of urbanization.