Detecting exogenous gene copy numbers is crucial for examining genetically modified organisms. Traditional methods face a trade-off between feasibility and cost, highlighting the need for a high-precision and low-cost...Detecting exogenous gene copy numbers is crucial for examining genetically modified organisms. Traditional methods face a trade-off between feasibility and cost, highlighting the need for a high-precision and low-cost alternative. In this study, we introduced the RISE method, which is based on Rare Allele Infusion and Sanger Sequencing Estimation, for rice.展开更多
At 2 a.m.on March 29,2023(Beijing Time),the Institute of High Energy Physics(IHEP)of the Chinese Academy of Sciences(CAS),together with some 40 research institutions worldwide,released their latest discoveries on the ...At 2 a.m.on March 29,2023(Beijing Time),the Institute of High Energy Physics(IHEP)of the Chinese Academy of Sciences(CAS),together with some 40 research institutions worldwide,released their latest discoveries on the brightest Gamma-Ray Burst(dubbed as GRB 221009A)ever detected by humanity.With the unique observations made by two Chinese space telescopes,namely Insight-HXMT and GECAM-C,scientists were able to accurately measure how bright and how much energy was released by this burst,which is the key to understanding this historical event.展开更多
从EMS诱变日本晴种子的M2代中筛选得到一个植株明显矮化、分蘖数急剧增多的突变体,命名为det1(dwarf and excessively-tillering1)。det1还显示出明显的穗型、粒型和育性等方面的生殖发育缺陷。遗传分析表明,det1受一对隐性基因控制。以...从EMS诱变日本晴种子的M2代中筛选得到一个植株明显矮化、分蘖数急剧增多的突变体,命名为det1(dwarf and excessively-tillering1)。det1还显示出明显的穗型、粒型和育性等方面的生殖发育缺陷。遗传分析表明,det1受一对隐性基因控制。以det1与品种DZ60杂交建立了F2定位群体,利用微卫星标记分析F2群体中的突变体,将DET1基因定位在第6染色体长臂端,标记SSR2和SSR3之间约68kb的范围内。已知该区间内存在一个与水稻株高和分蘖有关的基因D3。测序结果表明,DET1与D3是同一个基因,编码一个LRR型的F-box蛋白。与d3相比,det1还显示了新的表型特征,推测可能是等位基因的不同突变位点造成的。det1/d3的表型特征提示,DET1/D3在调节水稻生长发育过程中具有多效性。因此,利用det1开展进一步的研究将有助于全面揭示DET1/D3的分子功能。展开更多
基金supported by the Natural Science Foundation of Hunan Province for Distinguished Young Scholars, China (Grant No.2021JJ10041)the Biological Breeding-National Science and Technology Major Project, China (Grant No.2023ZD04072)the Hainan Yazhou Bay Seed Laboratory and National Seed Group, China (Grant Nos.B23CQ15HP and B23YQ1517)。
文摘Detecting exogenous gene copy numbers is crucial for examining genetically modified organisms. Traditional methods face a trade-off between feasibility and cost, highlighting the need for a high-precision and low-cost alternative. In this study, we introduced the RISE method, which is based on Rare Allele Infusion and Sanger Sequencing Estimation, for rice.
文摘At 2 a.m.on March 29,2023(Beijing Time),the Institute of High Energy Physics(IHEP)of the Chinese Academy of Sciences(CAS),together with some 40 research institutions worldwide,released their latest discoveries on the brightest Gamma-Ray Burst(dubbed as GRB 221009A)ever detected by humanity.With the unique observations made by two Chinese space telescopes,namely Insight-HXMT and GECAM-C,scientists were able to accurately measure how bright and how much energy was released by this burst,which is the key to understanding this historical event.
文摘从EMS诱变日本晴种子的M2代中筛选得到一个植株明显矮化、分蘖数急剧增多的突变体,命名为det1(dwarf and excessively-tillering1)。det1还显示出明显的穗型、粒型和育性等方面的生殖发育缺陷。遗传分析表明,det1受一对隐性基因控制。以det1与品种DZ60杂交建立了F2定位群体,利用微卫星标记分析F2群体中的突变体,将DET1基因定位在第6染色体长臂端,标记SSR2和SSR3之间约68kb的范围内。已知该区间内存在一个与水稻株高和分蘖有关的基因D3。测序结果表明,DET1与D3是同一个基因,编码一个LRR型的F-box蛋白。与d3相比,det1还显示了新的表型特征,推测可能是等位基因的不同突变位点造成的。det1/d3的表型特征提示,DET1/D3在调节水稻生长发育过程中具有多效性。因此,利用det1开展进一步的研究将有助于全面揭示DET1/D3的分子功能。
