Objective: To observe changes in RBC count, changes, RBC morphology, and serum potassium during compressed transfusion of WBC-removal whole blood. Methods: Prepared human WBC-removal whole blood and connected transf...Objective: To observe changes in RBC count, changes, RBC morphology, and serum potassium during compressed transfusion of WBC-removal whole blood. Methods: Prepared human WBC-removal whole blood and connected transfusion apparatus with different sizes of intravenous detaining needles (18G, 20G, 22G and 24G). Observed RBC count and serum potassium concentration under different pressures (100 mmHg, 200 mmHg, and 300 mmHg) as blood flowed out of the pinhead end of the intravenous detaining needle. Samples obtained with the 20G needle were smeared on glass slides, and RBC morphologic changes were observed under an oil immersion lens. Results: RBC count and serum potassium changed slightly under different pressures with different sizes of intravenous detaining needles as blood flowed through the transfusion apparatus. In addition, the observation of blood samples under a common light microscope revealed that coarse- prick, oblong, and spindle cell counts in the visual fields increased gradually as the pressure increased. Additionally, a portion of cells had undergone splintering. Conclusions: While applying 18G, 20G, 22G and 24G intravenous detaining needles for blood transfusion under less than 300 mmHg of pressure, no significant RBC count change was found in blood samples in the short term. However, there were significant RBC morphologic changes. The results could offer more basis to ensure the clinical safetv of oatients undereoing blood transfilsion.展开更多
Purpose: When hypertonic solution 20% mannitol solution was injected into vein, inflammatory mediators and Mitogen-activated protein kinases activated by mannitol can directly induce the fading of vascular endothelial...Purpose: When hypertonic solution 20% mannitol solution was injected into vein, inflammatory mediators and Mitogen-activated protein kinases activated by mannitol can directly induce the fading of vascular endothelial cell, which leads to phlebitis. The study aims to observe the influences of reparil-gel N coated at the proximal parts of the puncture point and basing on this along with infusing heated mannitol to veins to the injure and ultrastructure of veins which were infused the 20% mannitol solution by indwelling needle in vein. Methodology: There are 15 adult New Zealand rabbits. We randomly divided 24 ear veins of 12 adult New Zealand rabbits into Control group, Gelatum group, Gel heated group and injected 20% mannitol solution by vein detained needle in three groups. In Gelatum group, we coated the proximal end of the puncture point with a thin layer of compound aescine gel. Based on Gelatum group, we heated 20% mannitol solution to 35oC-37oC in Gel heated group. Then we observed the intravenous parts and took the veins of each group out to observe their morphology and ultrastructural after the second day of transfusion. 6 ear veins of the rest 3 rabbits as Health group weren’t given any interventions, the veins were taken out to observe their morphology. Results: Comparison between Gelatum group and Gelatum heated group on vascular dilatation, Infiltration of inflammatory cell and Formation of thrombus had no significance, P> 0.05, while the case was different for the comparison on injury of vascular wall, perivascular edema and perivascular hemorrhage, P< 0.05). The statistical significance exists between control group and Gelatum group and Gel heated group, P< 0.05. It was observed under the electron microscope that, in control group, the membrane of endothelial cell peeled off and the mitochondria swelled and vacuolized. In Gelatum group, the membrane of endothelial cell was defective, the parts of the mitochondria were fuzzy, intercellular substance was slightly edematous. In Gel heated group, the mitochondria were clear and intercellular substance slightly swelled. It could be found that the function of phagocyte was complete. Conclusions: Compound aescine gel can prevent phlebitis or reduce the incidence of phlebitis. The combined intervention of coating with a thin layer of compound aescine gel and heating mannitol solution can produce better effect.展开更多
Emerging evidence suggests that intron-detaining transcripts(IDTs)are a nucleus-detained and polyadenylated mRNA pool for cell to quickly and effectively respond to environmental stimuli and stress.However,the underly...Emerging evidence suggests that intron-detaining transcripts(IDTs)are a nucleus-detained and polyadenylated mRNA pool for cell to quickly and effectively respond to environmental stimuli and stress.However,the underlying mechanisms of detained intron(DI)splicing are still largely unknown.Here,we suggest that post-transcriptional DI splicing is paused at the Bact state,an active spliceosome but not catalytically primed,which depends on Smad Nuclear Interacting Protein 1(SNIP1)and RNPS1(a serine-rich RNA binding protein)interaction.RNPS1 and Bact components preferentially dock at DIs and the RNPS1 docking is sufficient to trigger spliceosome pausing.Haploinsufficiency of Snip1 attenuates neurodegeneration and globally rescues IDT accumulation caused by a previously reported mutant U2 snRNA,a basal spliceosomal component.Snip1 conditional knockout in the cerebellum decreases DI splicing efficiency and causes neurodegeneration.Therefore,we suggest that SNIP1 and RNPS1 form a molecular brake to promote spliceosome pausing,and that its misregulation contributes to neurodegeneration.展开更多
Cage-like octa(trimethylsiloxy)silsesquioxane [(Me3SiO)SiO1.5]8 has been synthesized via the trimethylsilylation of cubic tetramethylammonium silicate octamer [(Me4NO)-SiO1.5]8 with chlorotrimethylsilane. The silicate...Cage-like octa(trimethylsiloxy)silsesquioxane [(Me3SiO)SiO1.5]8 has been synthesized via the trimethylsilylation of cubic tetramethylammonium silicate octamer [(Me4NO)-SiO1.5]8 with chlorotrimethylsilane. The silicate octamer can be selectively formed by the reaction of tetraethoxysilane Si(OEt)4 with aqueous tetramethylammonium hydroxide in equal展开更多
Yahoo,我最喜欢浏览的网站,Oddly Enough是我最喜欢的专栏之一。这个栏目绝少有中国故事。不料,刚刚开学,就在Yahoo上读到北京大学生在动物园“作孽”的故事。“作孽”者往熊身上泼洒硫酸,后果严重,但他竟然解释:Iwanted to test whethe...Yahoo,我最喜欢浏览的网站,Oddly Enough是我最喜欢的专栏之一。这个栏目绝少有中国故事。不料,刚刚开学,就在Yahoo上读到北京大学生在动物园“作孽”的故事。“作孽”者往熊身上泼洒硫酸,后果严重,但他竟然解释:Iwanted to test whether or not they really are stupid。但是,I wanted to ask whetheror not he really is stupid!】展开更多
基金supported by the Shanxi Science and Technology Development Fund(No.200233)
文摘Objective: To observe changes in RBC count, changes, RBC morphology, and serum potassium during compressed transfusion of WBC-removal whole blood. Methods: Prepared human WBC-removal whole blood and connected transfusion apparatus with different sizes of intravenous detaining needles (18G, 20G, 22G and 24G). Observed RBC count and serum potassium concentration under different pressures (100 mmHg, 200 mmHg, and 300 mmHg) as blood flowed out of the pinhead end of the intravenous detaining needle. Samples obtained with the 20G needle were smeared on glass slides, and RBC morphologic changes were observed under an oil immersion lens. Results: RBC count and serum potassium changed slightly under different pressures with different sizes of intravenous detaining needles as blood flowed through the transfusion apparatus. In addition, the observation of blood samples under a common light microscope revealed that coarse- prick, oblong, and spindle cell counts in the visual fields increased gradually as the pressure increased. Additionally, a portion of cells had undergone splintering. Conclusions: While applying 18G, 20G, 22G and 24G intravenous detaining needles for blood transfusion under less than 300 mmHg of pressure, no significant RBC count change was found in blood samples in the short term. However, there were significant RBC morphologic changes. The results could offer more basis to ensure the clinical safetv of oatients undereoing blood transfilsion.
文摘Purpose: When hypertonic solution 20% mannitol solution was injected into vein, inflammatory mediators and Mitogen-activated protein kinases activated by mannitol can directly induce the fading of vascular endothelial cell, which leads to phlebitis. The study aims to observe the influences of reparil-gel N coated at the proximal parts of the puncture point and basing on this along with infusing heated mannitol to veins to the injure and ultrastructure of veins which were infused the 20% mannitol solution by indwelling needle in vein. Methodology: There are 15 adult New Zealand rabbits. We randomly divided 24 ear veins of 12 adult New Zealand rabbits into Control group, Gelatum group, Gel heated group and injected 20% mannitol solution by vein detained needle in three groups. In Gelatum group, we coated the proximal end of the puncture point with a thin layer of compound aescine gel. Based on Gelatum group, we heated 20% mannitol solution to 35oC-37oC in Gel heated group. Then we observed the intravenous parts and took the veins of each group out to observe their morphology and ultrastructural after the second day of transfusion. 6 ear veins of the rest 3 rabbits as Health group weren’t given any interventions, the veins were taken out to observe their morphology. Results: Comparison between Gelatum group and Gelatum heated group on vascular dilatation, Infiltration of inflammatory cell and Formation of thrombus had no significance, P> 0.05, while the case was different for the comparison on injury of vascular wall, perivascular edema and perivascular hemorrhage, P< 0.05). The statistical significance exists between control group and Gelatum group and Gel heated group, P< 0.05. It was observed under the electron microscope that, in control group, the membrane of endothelial cell peeled off and the mitochondria swelled and vacuolized. In Gelatum group, the membrane of endothelial cell was defective, the parts of the mitochondria were fuzzy, intercellular substance was slightly edematous. In Gel heated group, the mitochondria were clear and intercellular substance slightly swelled. It could be found that the function of phagocyte was complete. Conclusions: Compound aescine gel can prevent phlebitis or reduce the incidence of phlebitis. The combined intervention of coating with a thin layer of compound aescine gel and heating mannitol solution can produce better effect.
基金the Tsinghua-Peking Joint Center for Life Sciences,the Thousand-Talent Young Investigator Program,the IDG/McGovern Institute for Brain Research,the National Natural Science Foundation of China(81371361,92049114,31571097,82101495)The Beijing Municipal Science&Technology Commission(Z181100001518001,Z161100000216154)+1 种基金National Key R&D Program(2017YFC0110205)the Institute for Guo Qiang,Tsinghua University。
文摘Emerging evidence suggests that intron-detaining transcripts(IDTs)are a nucleus-detained and polyadenylated mRNA pool for cell to quickly and effectively respond to environmental stimuli and stress.However,the underlying mechanisms of detained intron(DI)splicing are still largely unknown.Here,we suggest that post-transcriptional DI splicing is paused at the Bact state,an active spliceosome but not catalytically primed,which depends on Smad Nuclear Interacting Protein 1(SNIP1)and RNPS1(a serine-rich RNA binding protein)interaction.RNPS1 and Bact components preferentially dock at DIs and the RNPS1 docking is sufficient to trigger spliceosome pausing.Haploinsufficiency of Snip1 attenuates neurodegeneration and globally rescues IDT accumulation caused by a previously reported mutant U2 snRNA,a basal spliceosomal component.Snip1 conditional knockout in the cerebellum decreases DI splicing efficiency and causes neurodegeneration.Therefore,we suggest that SNIP1 and RNPS1 form a molecular brake to promote spliceosome pausing,and that its misregulation contributes to neurodegeneration.
文摘Cage-like octa(trimethylsiloxy)silsesquioxane [(Me3SiO)SiO1.5]8 has been synthesized via the trimethylsilylation of cubic tetramethylammonium silicate octamer [(Me4NO)-SiO1.5]8 with chlorotrimethylsilane. The silicate octamer can be selectively formed by the reaction of tetraethoxysilane Si(OEt)4 with aqueous tetramethylammonium hydroxide in equal
文摘Yahoo,我最喜欢浏览的网站,Oddly Enough是我最喜欢的专栏之一。这个栏目绝少有中国故事。不料,刚刚开学,就在Yahoo上读到北京大学生在动物园“作孽”的故事。“作孽”者往熊身上泼洒硫酸,后果严重,但他竟然解释:Iwanted to test whether or not they really are stupid。但是,I wanted to ask whetheror not he really is stupid!】