The physiological functions of insect foregut,especially in xenobiotic detox ification,are scarcely reported because of unimportance in appearance and insufficient molecular information.The cockroach Periplaneta ameri...The physiological functions of insect foregut,especially in xenobiotic detox ification,are scarcely reported because of unimportance in appearance and insufficient molecular information.The cockroach Periplaneta americana,an entomological model organism,provides perfect material to study physiological functions of foregut tissue due to its architectuxal feature.Through Illumina sequencing of foregut tissue from P.amer icana individuals (control)or insects treated with cycloxaprid,as a novel neonicotinoid insecticide,54 193 166 clean reads were obtained and further assembled into 53 853 unigenes with an average length of 366 bp.Furthermore,the number of unigenes involved in xenobiofic detoxification was analyzed,mainly including 70 cytochrome P450s,12 glutathione S-transferases (GSTs),seven carboxylesterases (CarEs)and seven adenosine triphosphate-binding cassette (ABC)transporters.Compared to control,the expression of 22 xenobiotic detoxification unigenes was up-regulated after cycloxaprid application, mainly containing 18 P450s,one GST,two CarEs and one ABC adenosine triphosphate transporter,indicating that the oxidation-reduction was the major reactive process to cy cloxaprid application.Through quantitative real-time polymerase chitin reaction analysis, the expression of selected unigenes (six P450s,one GST and one CarE.)was up-regulated at least two-fold following cycloxaprid treatment,and was generally in agreement with transcriptome data.Compared to the previous midgut transcriptome of P.americana,it looks like the expressive abundance of the xenobiotic detoxification unigenes might be important factors to the detoxifying fimctional differences between foregut and midgut.In conclusion,insect foregut would also play important roles in the physiological processes related to xenobiotic detoxification.展开更多
基金the National Natural Science Foundation of China (31601656).
文摘The physiological functions of insect foregut,especially in xenobiotic detox ification,are scarcely reported because of unimportance in appearance and insufficient molecular information.The cockroach Periplaneta americana,an entomological model organism,provides perfect material to study physiological functions of foregut tissue due to its architectuxal feature.Through Illumina sequencing of foregut tissue from P.amer icana individuals (control)or insects treated with cycloxaprid,as a novel neonicotinoid insecticide,54 193 166 clean reads were obtained and further assembled into 53 853 unigenes with an average length of 366 bp.Furthermore,the number of unigenes involved in xenobiofic detoxification was analyzed,mainly including 70 cytochrome P450s,12 glutathione S-transferases (GSTs),seven carboxylesterases (CarEs)and seven adenosine triphosphate-binding cassette (ABC)transporters.Compared to control,the expression of 22 xenobiotic detoxification unigenes was up-regulated after cycloxaprid application, mainly containing 18 P450s,one GST,two CarEs and one ABC adenosine triphosphate transporter,indicating that the oxidation-reduction was the major reactive process to cy cloxaprid application.Through quantitative real-time polymerase chitin reaction analysis, the expression of selected unigenes (six P450s,one GST and one CarE.)was up-regulated at least two-fold following cycloxaprid treatment,and was generally in agreement with transcriptome data.Compared to the previous midgut transcriptome of P.americana,it looks like the expressive abundance of the xenobiotic detoxification unigenes might be important factors to the detoxifying fimctional differences between foregut and midgut.In conclusion,insect foregut would also play important roles in the physiological processes related to xenobiotic detoxification.
