Rapamycin increased development of CD4^+ CD25~h ighFoxp3^+ T cells of peripheral blood in liver transplant recipients

Objective To investigate the possible influence of immunosuppressive therapy,including sirolimus ( SRL) and calcineurin inhibitors ( CNI,tacrolimus) ,on level of Treg in liver allo - graft recipients. Methods Forty - seven liver transplant recipients with stable liver function were assessed for at least 2 years,and divided into

Total Quality Management at Algerian Higher Education Institutions to Promote Our Roles in Comprehensive Development

The aims this study to know the reality of the role for Total Quality Management(TQM)to need of Algerian Higher Education Institutions(AHEI)in apply the Total Quality Management(TQM)to promote the Comprehensive Development(CD),and this approach to enhance their roles in the comprehensive development,the efficiency of the training process,global changes and their needs to adopt a new educational policy in line with the global transformation competition,and the requirements of sustainable development,in this study was used a questionnaire composed of(75)items distributed on(08)areas,This is for confirmation the need to adopt philosophy of Total Quality Management(TQM)as a strategy imposed by the nature and the stage experienced for Algerian Higher Education Institutions(AHEI),And deal with the nature of the changes and challenges imposed by certain factors such as Globalization and the knowledge revolution,through all this inevitably to apply the Total Quality Management(TQM)in our Algerian Higher Education Institutions(AHEI).

幼儿教师儿向语言研究

儿童是在互动中习得语言的,与儿童互动的“涉儿群体”都有可能影响儿童语言的发展,其中就包括幼儿教师,其教学语言也可称为“儿向语言”。高质量儿向语言的运用与儿童良好语言习惯的形成、理解和表达能力的培养等密切相关,提高幼儿教师儿向语言的“发展性”,能将语言准备有机融入幼小过渡中,促进科学的幼小衔接。本文利用B站幼儿园教学视频和实录音频,从语音(音高、语速)、词汇(表疑问的句末语气助词、认识义情态词、人称代词)、句法(平均话语长度)、语用策略(提问类型)等方面考察幼儿教师儿向语言的纵向动态,揭示出该群体儿向语言“发展性”不足的问题,即基频、语速不会随儿童年级变化而明显调整,词汇使用仅适用较低的认知发展水平,平均话语长度调整不显著,封闭性提问占主导等。据此提出针对性改进策略:在大班下学期幼儿教师可以适当提高平均语速,降低平均基频,提高句法复杂度,增加开放性提问等。

草鱼、中华鳖T淋巴细胞表面抗原的研究

以草鱼脑组织、中华鳖脑组织和胸腺细胞为抗原制备兔抗草鱼脑血清 (RACBS)、兔抗中华鳖脑血清 (RATBS)和兔抗中华鳖胸腺细胞血清 (RATTS)。补体依赖性细胞毒试验和不同组织对RATBS、RATTS的吸收试验结果表明 :中华鳖胸腺细胞和脑组织均存在Thy1抗原(亦称脑组织抗原或胸腺 -脑组织T细胞抗原 ) ;草鱼脑组织缺乏Thy1抗原。应用间接酶标免疫组化染色技术显示 :Thy1抗原阳性反应物沉淀于中华鳖胸腺细胞和外周一部分淋巴细胞表面。进一步用抗人白细胞分化抗原CD4、CD8单克隆抗体进行免疫组化交叉反应提示中华鳖淋巴细胞膜上含有与人类T淋巴细胞表面抗原CD4、CD8类似结构的物质。本文讨论了Thy1抗原、CD抗原的出现及其意义 ,探讨了淋巴细胞异质性及RATBS、RATTS的特异性和作用。

草鱼、中华鳖脾细胞培养上清液IL-2物质的检测

用刀豆蛋白A(ConA)刺激诱导草鱼和中华鳖脾细胞 ,收集细胞培养上清液 ,用小鼠胸腺细胞增殖试验和对小鼠L92 9细胞系杀伤试验检测上清液中白细胞介素 2 (简称IL 2 )活性。结果表明 :草鱼、中华鳖脾细胞培养上清液中有IL 2样活性物质 ,这种物质使小鼠的胸腺细胞3H TdR掺入量明显增加 ,在相同效靶比的条件下对小鼠L92 9细胞系杀伤率也显著增强 ,这种IL 2活性均能被抗人rIL 2血清所抑制。中华鳖脾细胞培养上清液 (含IL 2 )对中华鳖胸腺细胞也有较明显的促增殖作用并能消除兔抗中华鳖胸腺细胞血清 (RATTS)对中华鳖胸腺细胞增殖的抑制作用。进一步用抗人白细胞分化抗原CD2 5(IL 2受体 ,简称IL 2R)单克隆抗体进行免疫组化交叉反应提示草鱼、中华鳖淋巴细胞膜上含有与人类IL 2R类似功能和结构的物质。