Future Development Direction of the Restoration of Mountains,Rivers,Forests,Farmlands,Lakes,and Grasslands and the Faced Problems

In this paper,the definition,connotation,and internal relationship of mountains,rivers,forests,farmlands,lakes,and grasslands in China are elaborated,and the current situation of ecological restoration projects for mountains,rivers,forests,farmlands,lakes,and grasslands was introduced.Moreover,the problems that have arisen in the specific implementation process of pilot projects were analyzed,such as unclear target positioning,inaccurate analysis of ecological problems,insufficient engineering design systematicness,weak operability of evaluation standards,and weak coordination in engineering management.The development direction and major needs for the protection and restoration of mountains,rivers,forests,farmlands,lakes,and grasslands in the future have been proposed from four aspects:theoretical research,engineering design,effect evaluation,and monitoring and supervision.

China’s Industrial Modernization:Development Rationale,Current Status,and Policy Directions

Economies that have effectively escaped the“middle-income trap”demonstrate common traits in their industrial restructuring as they progressed to high-income status.These include a relatively stable share of an economy’s manufacturing sector,a reasonable economic structure,enhanced industrial capabilities,and growth driven by innovation.However,late-moving countries face a number of hurdles as they strive to cross this threshold.China’s development advantages include,among other things,a complete industrial system,a more balanced industrial structure,growing indigenous innovation capabilities,continual expansion and upgrading of domestic demand,and a greater degree of openness.These capabilities have provided continuous momentum for industrial growth,allowing China to capitalize on the next wave of technological and industrial revolutions while also promoting long-term,steady industrial development.During its modernization efforts,China has seen substantial changes in the external environment surrounding its industrial development.We must not only recognize the increasing complexity,intensity,and uncertainty of these changes,but also take proactive steps to solve diverse issues and capitalize on opportunities arising from global digital and green transitions.Equal focus should be placed on strengthening reforms and promoting high-level openness,improving policy coordination and consistency,and pursuing an innovation-driven strategy.This will speed the development of a modern industrial system and encourage the formation of new,high-quality productive forces.

New aspects of a small GTPase RAB35 in brain development and function

In eukaryotic cells,organelles in the secretory,lysosomal,and endocytic pathways actively exchange biological materials with each other through intracellular membrane trafficking,which is the process of transporting the cargo of proteins,lipids,and other molecules to appropriate compartments via transport vesicles or intermediates.These processes are strictly regulated by various small GTPases such as the RAS-like in rat brain(RAB)protein family,which is the largest subfamily of the RAS superfamily.Dysfunction of membrane trafficking affects tissue homeostasis and leads to a wide range of diseases,including neurological disorders and neurodegenerative diseases.Therefore,it is important to understand the physiological and pathological roles of RAB proteins in brain function.RAB35,a member of the RAB family,is an evolutionarily conserved protein in metazoans.A wide range of studies using cultured mammalian cells and model organisms have revealed that RAB35 mediates various processes such as cytokinesis,endocytic recycling,actin bundling,and cell migration.RAB35 is also involved in neurite outgrowth and turnover of synaptic vesicles.We generated brain-specific Rab35 knockout mice to study the physiological roles of RAB35 in brain development and function.These mice exhibited defects in anxiety-related behaviors and spatial memory.Strikingly,RAB35 is required for the precise positioning of pyramidal neurons during hippocampal development,and thereby for normal hippocampal lamination.In contrast,layer formation in the cerebral cortex occurred superficially,even in the absence of RAB35,suggesting a predominant role for RAB35 in hippocampal development rather than in cerebral cortex development.Recent studies have suggested an association between RAB35 and neurodegenerative diseases,including Parkinson's disease and Alzheimer's disease.In this review,we provide an overview of the current understanding of subcellular functions of RAB35.We also provide insights into the physiological role of RAB35 in mammalian brain development and function,and discuss the involvement of RAB35 dysfunction in neurodegenerative diseases.

Establishment of human cerebral organoid systems to model early neural development and assess the central neurotoxicity of environmental toxins

Human brain development is a complex process,and animal models often have significant limitations.To address this,researchers have developed pluripotent stem cell-derived three-dimensional structures,known as brain-like organoids,to more accurately model early human brain development and disease.To enable more consistent and intuitive reproduction of early brain development,in this study,we incorporated forebrain organoid culture technology into the traditional unguided method of brain organoid culture.This involved embedding organoids in matrigel for only 7 days during the rapid expansion phase of the neural epithelium and then removing them from the matrigel for further cultivation,resulting in a new type of human brain organoid system.This cerebral organoid system replicated the temporospatial characteristics of early human brain development,including neuroepithelium derivation,neural progenitor cell production and maintenance,neuron differentiation and migration,and cortical layer patterning and formation,providing more consistent and reproducible organoids for developmental modeling and toxicology testing.As a proof of concept,we applied the heavy metal cadmium to this newly improved organoid system to test whether it could be used to evaluate the neurotoxicity of environmental toxins.Brain organoids exposed to cadmium for 7 or 14 days manifested severe damage and abnormalities in their neurodevelopmental patterns,including bursts of cortical cell death and premature differentiation.Cadmium exposure caused progressive depletion of neural progenitor cells and loss of organoid integrity,accompanied by compensatory cell proliferation at ectopic locations.The convenience,flexibility,and controllability of this newly developed organoid platform make it a powerful and affordable alternative to animal models for use in neurodevelopmental,neurological,and neurotoxicological studies.

Postnatal development of rat retina:a continuous observation and comparison between the organotypic retinal explant model and in vivo development

The organotypic retinal explant culture has been established for more than a decade and offers a range of unique advantages compared with in vivo experiments and cell cultures.However,the lack of systematic and continuous comparison between in vivo retinal development and the organotypic retinal explant culture makes this model controversial in postnatal retinal development studies.Thus,we aimed to verify the feasibility of using this model for postnatal retinal development studies by comparing it with the in vivo retina.In this study,we showed that postnatal retinal explants undergo normal development,and exhibit a consistent structure and timeline with retinas in vivo.Initially,we used SOX2 and PAX6 immunostaining to identify retinal progenitor cells.We then examined cell proliferation and migration by immunostaining with Ki-67 and doublecortin,respectively.Ki-67-and doublecortin-positive cells decreased in both in vivo and explants during postnatal retinogenesis,and exhibited a high degree of similarity in abundance and distribution between groups.Additionally,we used Ceh-10 homeodomain-containing homolog,glutamate-ammonia ligase(glutamine synthetase),neuronal nuclei,and ionized calcium-binding adapter molecule 1 immunostaining to examine the emergence of bipolar cells,Müller glia,mature neurons,and microglia,respectively.The timing and spatial patterns of the emergence of these cell types were remarkably consistent between in vivo and explant retinas.Our study showed that the organotypic retinal explant culture model had a high degree of consistency with the progression of in vivo early postnatal retina development.The findings confirm the accuracy and credibility of this model and support its use for long-term,systematic,and continuous observation.

Influences of Financial Development and Energy Price on Renewable Energy:An Italian Case

Global climate change has created substantial difficulties in the areas of sustainability,development,and environmental conservation due to the widespread dependence on fossil fuels for energy production.Nevertheless,the promotion of renewable energy programs has the potential to significantly expedite endeavors aimed at tackling climate change.Thus,it is essential to conduct a thorough analysis that considers the financial aspects to fully understand the main hurdles that are preventing the advancement of renewable energy initiatives.Italy is a leading country in the worldwide deployment of renewable energy.The objective of this research is to assess the impact of financial growth,economic progress,and energy expenses on Italy’s adoption of renewable energy sources.By employing the Auto-Regressive Distributed Lag(ARDL)technique,we analyzed annual data spanning from1990 to 2022.Findings revealed that a 1%increase in financial and economic development would boost renewable energy consumption in the long run by 0.29%and 0.48%,respectively.Instead,a 1%increase in energy prices might reduce consumption of renewable energy by 0.05%in the long run.This study’s primary significance lies in furnishing actionable strategies for Italy to augment green finance for renewable energy,fostering sustained social and economic progress.Moreover,the analytical insights gleaned from this research offer valuable insights for energy-importing nations worldwide.

Exploring the dynamics of creative tourism:A tourist-centric perspective to achieve economic sustainable development

Creative tourism is a dynamic and innovative approach to tourism,which points out the importance of people's active participation and their immersion in such experiences.In a vernacular context,it should attract people(local and tourists)attention to accomplish its main goals.Despite its rich cultural and natural assets,Kurdistan province faces several challenges that impact its tourism potential.To achieve that,the study uses quantitative approach to thoroughly analyze and evaluate the components of creative tourism in this province.The research focuses on tourists who visited the province's ten towns during the spring and summer of 2023.Data collection utilized a Likert-scale questionnaire ranging from"very good"to"very poor".The study employed a semistructured questionnaire developed through qualitative interviews alongside a researcher-made questionnaire validated by experts from the University of Kurdistan.The qualitative questionnaire achieved a high-reliability score of 93%using Cronbach's alpha coefficient.In-depth interviews and literary research were conducted to identify creative tourism components and indicators,informing the development of a quantitative questionnaire.Data analysis was performed using SPSS 20 and AMOS software to scrutinize the survey findings,providing insights into enhancing creative tourism strategies in Kurdistan province.The results reveal the varying significance of these dimensions,with the cultural dimension identified as the most crucial(factor loading:0.95),followed by the social(0.92),economic(0.88),and managerial/political dimensions(0.83).The study highlights the importance of cultural planning,community engagement,and infrastructural support in fostering creative tourism.Furthermore,it explores the impact of creative industries,such as music and arts rooted in Kurdish culture,on tourism development.Economic diversification and spatialphysical considerations are critical factors in enhancing Kurdistan's appeal as a creative tourism destination,emphasizing sustainable growth and cultural preservation.

Numerical simulation of microstructure and microporosity morphology in directional solidification of aluminum-copper alloys:Effect of copper content and withdrawal rate

Microporosity formed in the solidification process of Al alloys is detrimental to the alloy properties.A two-dimensional cellular automaton(CA)model was developed to simulate the microstructure and microporosity formation in Al-Cu alloys,considering variations in Cu content and solidification rate.The results indicate that the Cu content primarily influences the growth of microporosity.To validate the model,directional solidification experiments were conducted on Al-Cu alloys with varing Cu contents and withdrawal rates.The experimental results of dendrites and microporosity characteristics agree well with the predictions from the developed model,thus confirming the validity of the model.The alloy’s liquidus temperature,dendrite morphology,and hydrogen saturation solubility arising from different Cu contents have significant effects on microporosity morphology.The withdrawal rate primarily affects the nucleation of hydrogen microporosity by altering cooling rates and dendritic growth rates,resulting in different microporosity characteristics.

Leucine-Rich Repeat Protein Family Regulates Stress Tolerance and Development in Plants

The leucine-rich repeat(LRR)protein family is involved in a variety of fundamental metabolic and signaling processes in plants,including growth and defense responses.LRR proteins can be divided into two categories:those containing LRR domains along with other structural elements,which are further subdivided into five groups,LRR receptor-like kinases,LRR receptor-like proteins,nucleotide-binding site LRR proteins,LRR-extensin proteins,and polygalacturonase-inhibiting proteins,and those containing only LRR domains.Functionally,various LRR proteins are primarily involved in plant development and responses to environmental stress.Notably,the LRR protein family plays a central role in signal transduction pathways related to stress adaptation.In this review,we classify and analyze the functions of LRR proteins in plants.While extensive research has been conducted on the roles of LRR proteins in disease resistance signaling,these proteins also play important roles in abiotic stress responses.This review highlights recent advances in understanding how LRR proteins mediate responses to biotic and abiotic stresses.Building upon these insights,further exploration of the roles of LRR proteins in abiotic stress resistance may aid efforts to develop rice varieties with enhanced stress and disease tolerance.

Progress and development directions of stimulation techniques for ultra-deep oil and gas reservoirs

By reviewing the development history of stimulation techniques for deep/ultra-deep oil and gas reservoirs,the new progress in this field in China and abroad has been summed up,including deeper understanding on formation mechanisms of fracture network in deep/ultra-deep oil and gas reservoir,performance improvement of fracturing fluid materials,fine stratification of ultra-deep vertical wells,and mature staged multi-cluster fracturing technique for ultra-deep and highly deviated wells/horizontal wells.In light of the exploration and development trend of ultra-deep oil and gas reservoirs in China,the requirements and technical difficulties in ultra-deep oil and gas reservoir stimulation are discussed:(1)The research and application of integrated geological engineering technology is difficult.(2)The requirements on fracturing materials for stimulation are high.(3)It is difficult to further improve the production in vertical profile of the ultra-deep and hugely thick reservoirs.(4)The requirements on tools and supporting high-pressure equipment on the ground for stimulation are high.(5)It is difficult to achieve efficient stimulation of ultra-deep,high-temperature and high-pressure wells.(6)It is difficult to monitor directly the reservoir stimulation and evaluate the stimulation effect accurately after stimulation.In line with the complex geological characteristics of ultra-deep oil and gas reservoirs in China,seven technical development directions are proposed:(1)To establish systematic new techniques for basic research and evaluation experiments;(2)to strengthen geological research and improve the operational mechanism of integrating geological research and engineering operation;(3)to develop high-efficiency fracturing materials for ultra-deep reservoirs;(4)to research separated layer fracturing technology for ultra-deep and hugely thick reservoirs;(5)to explore fracture-control stimulation technology for ultra-deep horizontal well;(6)to develop direct monitoring technology for hydraulic fractures in ultra-deep oil and gas reservoirs;(7)to develop downhole fracturing tools with high temperature and high pressure tolerance and supporting wellhead equipment able to withstand high pressure.

Distribution characteristics, exploration and development, geological theories research progress and exploration directions of shale gas in China

The shale gas resources in China have great potential and the geological resources of shale gas is over 100×10^(12)m^(3),which includes about 20×10^(12)m^(3) of recoverable resources.Organic-rich shales can be divided into three types according to their sedimentary environments,namely marine,marine-continental transitional,and continental shales,which are distributed in 13 stratigraphic systems from the Mesoproterozoic to the Cenozoic.The Sichuan Basin and its surrounding areas have the highest geological resources of shale gas,and the commercial development of shale gas has been achieved in the Upper Ordovician Wufeng Formation-Lower Silurian Longmaxi Formation in these areas,with a shale gas production of up to 20×10^(9)m^(3) in 2020.China has seen rapid shale gas exploration and development over the last five years,successively achieving breakthroughs and important findings in many areas and strata.The details are as follows.(1)Large-scale development of middle-shallow shale gas(burial depth:less than 3500 m)has been realized,with the productivity having rapidly increased;(2)breakthroughs have been constantly made in the development of deep shale gas(burial depth:3500-4500 m),and the ultradeep shale gas(burial depth:greater than 4500 m)is under testing;(3)breakthroughs have been made in the development of normal-pressure shale gas,and the assessment of the shale gas in complex tectonic areas is being accelerated;(4)shale gas has been frequently discovered in new areas and new strata,exhibiting a great prospect.Based on the exploration and development practice,three aspects of consensus have been gradually reached on the research progress in the geological theories of shale gas achieved in China.(1)in terms of deep-water fine-grained sediments,organic-rich shales are the base for the formation of shale gas;(2)in terms of high-quality reservoirs,the development of micro-nano organic matter-hosted pores serves as the core of shale gas accumulation;(3)in terms of preservation conditions,weak structural transformation,a moderate degree of thermal evolution,and a high pressure coefficient are the key to shale gas enrichment.As a type of important low-carbon fossil energy,shale gas will play an increasingly important role in achieving the strategic goals of peak carbon dioxide emissions and carbon neutrality.Based on the in-depth study of shale gas geological conditions and current exploration progress,three important directions for shale gas exploration in China in the next five years are put forward.

Technical status and development direction of workover operation of PetroChina

Through a comprehensive review of the development status of workover technology of PetroChina Company Limited(PetroChina), this paper presents the connotation of workover operation under the background of the new era, the latest progress of workover operation in the respects of equipment, tools, technology and the construction of information and standardization. The gaps between PetroChina and foreign counterpart in workover technology are as follows: the level of automation and intellectualization of tools and equipment is relatively low, the snubbing operation in gas wells characterized by HT/HP and high H2 S is lagged behind;water plugging in the long horizontal wellbore needs to be further developed, coiled tubing and its relevant equipment for ultra-deep well operation has to be optimized;informationization, standardization and big data application of workover operation need to be started. Based on this as well as the development status of workover technology in China and the technical difficulties faced in the future, eight suggestions for future development are put forward:(1) strengthen the dynamic understanding of reservoir and improve the pertinence of workover schemes;(2) develop the general overhaul technology in a systematical way to tackle issues of seriously problematic wells;(3) put more efforts into the research of horizontal well workover operation and develop relevant technology for long horizontal section operation;(4) improve the snubbing technology and extend its applications;(5) expand the capacity of coiled tubing operation and improve the level of special operations;(6) develop automatic workover technology into the field of artificial intelligence;(7) promote clean operation in an all-round way and build green oil and gas fields;(8) perfect the informationization construction to realize the sharing of workover resources.

Technological progress and development directions of PetroChina overseas oil and gas field production

This study reviews the development history of PetroChina’s overseas oil and gas field development technologies, summarizes the characteristic technologies developed, and puts forward the development goals and technological development directions of overseas business to overcome the challenges met in overseas oil and gas production. In the course of PetroChina’s overseas oil and gas field production practice of more than 20 years, a series of characteristic technologies suitable for overseas oil and gas fields have been created by combining the domestic mature oil and gas field production technologies with the features of overseas oil and gas reservoirs, represented by the technology for high-speed development and stabilizing oil production and controlling water rise for overseas sandstone oilfields, high efficiency development technology for large carbonate oil and gas reservoirs and foamy oil depletion development technology in use of horizontal wells for extra-heavy oil reservoirs. Based on in-depth analysis of the challenges faced by overseas oil and gas development and technological requirements, combined with the development trends of oil and gas development technologies in China and abroad, overseas oil and gas development technologies in the future are put forward, including artificial intelligence reservoir prediction and 3 D geological modeling, secondary development and enhanced oil recovery(EOR) of overseas sandstone oilfields after high speed development, water and gas injection to improve oil recovery in overseas carbonate oil and gas reservoirs, economic and effective development of overseas unconventional oil and gas reservoirs, efficient development of marine deep-water oil and gas reservoirs. The following goals are expected to be achieved: keep the enhanced oil recovery(EOR) technology for high water-cut sandstone oilfield at international advanced level, and make the development technology for carbonate oil and gas reservoirs reach the international advanced level, and the development technologies for unconventional and marine deep-water oil and gas reservoirs catch up the level of international leading oil companies quickly.

Application and development of numerical simulation for under- ground horizontal directional drilling

In coalmines of China, horizontal directional drilling （HDD） is an increasingly popular method for underground in-seam gas drainage. Numerical simulation, especially finite element analysis, is often used as an effective method to improve HDD operation. These improvements focus on rock-breaking efficiency, directional precision, stability of the borehole wall, and reliability of the drill equipment. On the basis of underground drilling characteristics, typical numerical simulation exam- ples in drilling techniques and equipment are summarized and analyzed. In the end, the future development trends of numerical simulation in underground in-seam drilling are proposed.

Progress and Developmental Direction of Post-harvest Component Detection and Preservation Techniques of Nanfeng Citrus in China

This paper was summarized the research status and the development tendency of post-harvest component detection and preservation techniques of Nanfeng citrus in China, also analyzed the characteristics of various techniques, and proposed the developmental direction of novel techniques for post-harvest component detection and preservation of Nanfeng citrus.

Supply chain management research directions for future development

With the rapid development of electronic commerce, supply chain management is facing more and more new challenges. Therefore, it is particularly important to study the development of clear direction of supply chain. However, the supply chain is composed of different interest subjects, each subject in order to achieve the maximization of their own interests and adopt different measures, is a complex, huge system, has brought the huge challenge to the supply chain management. In this paper, the research on Supply Chain Management in the current situation, characteristics and the modem supply chain management are summarized, and combined with new challenges of supply chain management to predict future research direction is the development of supply chain management, in order to make the supply chain management to the greatest extent satisfy the consumer e-commerce mode increasingly popular.

Research on the Modern Music Composition Development Direction under the Digital and Computer Background

In this paper, we conduct research on modem music composition development direction under the digital and computer background. We can hear the music work, at ordinary times is the musician and sound engineer through three different stages of product created by mutual cooperation. The development of computer music has impact to the music every aspect of life, including various music creation process, cause the changes of some design principles. This change may be denied the traditional, also may be denied the traditional that is on the development of traditional principles. We integrate the concepts and principles of the digital perspective to propose our methodology on issues of computer music develooment that holds vital siznificance and meaning.

Research on the Development Direction of Traditional Broadcast Television Movie Media under Background of New Media and Internet

In this paper, we conduct research on the development direction of traditional broadcast television movie media under background of new media and lnternet. In recent years, with the computer technology network communication technology and constant innovation rapid development of digital technology, digital new media got rapid development. Under the digital new media industry has gradually become the core industry of 21 st century knowledge economy and the new economic growth point. But there are still some problems in the digital of new media development. How to treat these problems to take effective countermeasures is vital to healthy development of the new media. Our research proposes corresponding guidance and the perspective on the development orientation which acts as the paradigm and the guideline.