Farmers' professional cooperatives develop in various forms,and with the continuous improvement of development level,social effects have gradually appear.Based on the specific situation of farmers' professiona...Farmers' professional cooperatives develop in various forms,and with the continuous improvement of development level,social effects have gradually appear.Based on the specific situation of farmers' professional cooperatives in Shangqiu City,this paper expounded the development status of farmers' professional cooperatives in Shangqiu City,analyzed and discussed various problems encountered in the development process of farmers' professional cooperatives,and put forward some countermeasures and suggestions,such as establishing and perfecting cooperative rules and regulations,financing from multiple channels,introducing technical talents in various ways,breaking the information asymmetry restriction by various means,and giving full play to government functions,aiming to further promote standardized,large-scale,healthy and sustainable development of farmers' professional cooperatives.展开更多
Utilizing energy storage in depleted oil and gas reservoirs can improve productivity while reducing power costs and is one of the best ways to achieve synergistic development of"Carbon Peak–Carbon Neutral"a...Utilizing energy storage in depleted oil and gas reservoirs can improve productivity while reducing power costs and is one of the best ways to achieve synergistic development of"Carbon Peak–Carbon Neutral"and"Underground Resource Utiliza-tion".Starting from the development of Compressed Air Energy Storage(CAES)technology,the site selection of CAES in depleted gas and oil reservoirs,the evolution mechanism of reservoir dynamic sealing,and the high-flow CAES and injection technology are summarized.It focuses on analyzing the characteristics,key equipment,reservoir construction,application scenarios and cost analysis of CAES projects,and sorting out the technical key points and existing difficulties.The devel-opment trend of CAES technology is proposed,and the future development path is scrutinized to provide reference for the research of CAES projects in depleted oil and gas reservoirs.展开更多
In recent years,with the support and promotion of relevant policies such as the Opinions on Promoting Study Tour for Primary and Secondary School Students jointly issued by the Ministry of Education and 11 other depar...In recent years,with the support and promotion of relevant policies such as the Opinions on Promoting Study Tour for Primary and Secondary School Students jointly issued by the Ministry of Education and 11 other departments,a wave of study tour has been sparked nationwide from individual pilot projects to gradual promotion.In this paper,students and teachers from grades 4 to 6 of L Primary School in Nanchang City,Jiangxi Province were selected as the survey objects.Combining the survey questionnaire,the current situation of conducting study tour in the primary school was sorted out and analyzed in depth from several aspects such as study tour cognition,study tour participation,study tour implementation,etc.Based on the survey results and relevant research,the current problems in conducting study tours in primary schools were summarized,and targeted improvement measures were proposed,in order to promote the healthy development of study tours in primary schools.展开更多
Economies that have effectively escaped the“middle-income trap”demonstrate common traits in their industrial restructuring as they progressed to high-income status.These include a relatively stable share of an econ...Economies that have effectively escaped the“middle-income trap”demonstrate common traits in their industrial restructuring as they progressed to high-income status.These include a relatively stable share of an economy’s manufacturing sector,a reasonable economic structure,enhanced industrial capabilities,and growth driven by innovation.However,late-moving countries face a number of hurdles as they strive to cross this threshold.China’s development advantages include,among other things,a complete industrial system,a more balanced industrial structure,growing indigenous innovation capabilities,continual expansion and upgrading of domestic demand,and a greater degree of openness.These capabilities have provided continuous momentum for industrial growth,allowing China to capitalize on the next wave of technological and industrial revolutions while also promoting long-term,steady industrial development.During its modernization efforts,China has seen substantial changes in the external environment surrounding its industrial development.We must not only recognize the increasing complexity,intensity,and uncertainty of these changes,but also take proactive steps to solve diverse issues and capitalize on opportunities arising from global digital and green transitions.Equal focus should be placed on strengthening reforms and promoting high-level openness,improving policy coordination and consistency,and pursuing an innovation-driven strategy.This will speed the development of a modern industrial system and encourage the formation of new,high-quality productive forces.展开更多
The efficacy,application and compatibility of five kinds of Chinese Herbal Medicines medicine(including yam,licorice,Platyc-odonisi Radix,Polygonati Rhizoma,and Pueraria Lobata)for medicine and food homology in the pa...The efficacy,application and compatibility of five kinds of Chinese Herbal Medicines medicine(including yam,licorice,Platyc-odonisi Radix,Polygonati Rhizoma,and Pueraria Lobata)for medicine and food homology in the past five years were systematically searched and summarized,and the current situation of their food development was reviewed to provide theoretical basis for the research and development of this kind of CHMs for health care.展开更多
According to the"National Modern Facility Agriculture Construction Plan(2023-2030)released by the Ministry of Agriculture and Rural Affairs,the"Special Implementation Plan for Modern Facility Fisheries Const...According to the"National Modern Facility Agriculture Construction Plan(2023-2030)released by the Ministry of Agriculture and Rural Affairs,the"Special Implementation Plan for Modern Facility Fisheries Construction"emphasizes the development of circular aquaculture,active expansion of green aquaculture space in facility fisheries,multi-channel development of food sources,comprehensive improvement of the quality,efficiency,and competitiveness of facility fisheries,ensuring stable and safe supply of aquatic products,and better meeting people's needs for high-quality aquatic products and beautiful aquatic ecological environment.Since the implementation of the 14th Five Year Plan for fisheries,facility fisheries have made significant progress.In order to thoroughly implement General Secretary Xi Jinping's important discourse on new quality productivity,conduct in-depth research on the current situation and existing problems of the fishery industry,and put forward suggestions to meet the requirements of high-quality development of the fishery industry in the new era.Therefore,it is crucial to continue practicing the"revitalization of aquatic seed industry"and"big food concept",take multiple measures to"demand food from rivers,lakes,and seas",and expand aquaculture space.Through research,it has been found that research in the field of facility based fisheries is still in its developmental stage.Further exploration is needed on the current status,breeding models,and experimental demonstrations of land-based facility based fisheries,in order to provide a basis for fisheries regulatory authorities to formulate the"15th Five Year Plan"for facility based fisheries.展开更多
In eukaryotic cells,organelles in the secretory,lysosomal,and endocytic pathways actively exchange biological materials with each other through intracellular membrane trafficking,which is the process of transporting t...In eukaryotic cells,organelles in the secretory,lysosomal,and endocytic pathways actively exchange biological materials with each other through intracellular membrane trafficking,which is the process of transporting the cargo of proteins,lipids,and other molecules to appropriate compartments via transport vesicles or intermediates.These processes are strictly regulated by various small GTPases such as the RAS-like in rat brain(RAB)protein family,which is the largest subfamily of the RAS superfamily.Dysfunction of membrane trafficking affects tissue homeostasis and leads to a wide range of diseases,including neurological disorders and neurodegenerative diseases.Therefore,it is important to understand the physiological and pathological roles of RAB proteins in brain function.RAB35,a member of the RAB family,is an evolutionarily conserved protein in metazoans.A wide range of studies using cultured mammalian cells and model organisms have revealed that RAB35 mediates various processes such as cytokinesis,endocytic recycling,actin bundling,and cell migration.RAB35 is also involved in neurite outgrowth and turnover of synaptic vesicles.We generated brain-specific Rab35 knockout mice to study the physiological roles of RAB35 in brain development and function.These mice exhibited defects in anxiety-related behaviors and spatial memory.Strikingly,RAB35 is required for the precise positioning of pyramidal neurons during hippocampal development,and thereby for normal hippocampal lamination.In contrast,layer formation in the cerebral cortex occurred superficially,even in the absence of RAB35,suggesting a predominant role for RAB35 in hippocampal development rather than in cerebral cortex development.Recent studies have suggested an association between RAB35 and neurodegenerative diseases,including Parkinson's disease and Alzheimer's disease.In this review,we provide an overview of the current understanding of subcellular functions of RAB35.We also provide insights into the physiological role of RAB35 in mammalian brain development and function,and discuss the involvement of RAB35 dysfunction in neurodegenerative diseases.展开更多
Faced with unprecedented changes in the world,China has chosen to continue expanding its opening-up and actively cooperate with other countries.Internationalization of education is one of the important directions for ...Faced with unprecedented changes in the world,China has chosen to continue expanding its opening-up and actively cooperate with other countries.Internationalization of education is one of the important directions for the future development of universities.Since the reform and opening-up,a series of Sino-foreign cooperative education policies have been successively released,forming a relatively systematic and complete policy system.Under the promotion of policies,Chinese universities actively engage in Sino-foreign cooperative education,with increasingly frequent educational exchanges and a clear trend towards internationalization.The scale of Sino-foreign cooperative education is also constantly expanding.It is worthy to explore how to draw on advanced teaching experience from overseas,continue to promote Sino-foreign cooperative education,and improve the quality of Sino-foreign cooperative education.展开更多
Human brain development is a complex process,and animal models often have significant limitations.To address this,researchers have developed pluripotent stem cell-derived three-dimensional structures,known as brain-li...Human brain development is a complex process,and animal models often have significant limitations.To address this,researchers have developed pluripotent stem cell-derived three-dimensional structures,known as brain-like organoids,to more accurately model early human brain development and disease.To enable more consistent and intuitive reproduction of early brain development,in this study,we incorporated forebrain organoid culture technology into the traditional unguided method of brain organoid culture.This involved embedding organoids in matrigel for only 7 days during the rapid expansion phase of the neural epithelium and then removing them from the matrigel for further cultivation,resulting in a new type of human brain organoid system.This cerebral organoid system replicated the temporospatial characteristics of early human brain development,including neuroepithelium derivation,neural progenitor cell production and maintenance,neuron differentiation and migration,and cortical layer patterning and formation,providing more consistent and reproducible organoids for developmental modeling and toxicology testing.As a proof of concept,we applied the heavy metal cadmium to this newly improved organoid system to test whether it could be used to evaluate the neurotoxicity of environmental toxins.Brain organoids exposed to cadmium for 7 or 14 days manifested severe damage and abnormalities in their neurodevelopmental patterns,including bursts of cortical cell death and premature differentiation.Cadmium exposure caused progressive depletion of neural progenitor cells and loss of organoid integrity,accompanied by compensatory cell proliferation at ectopic locations.The convenience,flexibility,and controllability of this newly developed organoid platform make it a powerful and affordable alternative to animal models for use in neurodevelopmental,neurological,and neurotoxicological studies.展开更多
The organotypic retinal explant culture has been established for more than a decade and offers a range of unique advantages compared with in vivo experiments and cell cultures.However,the lack of systematic and contin...The organotypic retinal explant culture has been established for more than a decade and offers a range of unique advantages compared with in vivo experiments and cell cultures.However,the lack of systematic and continuous comparison between in vivo retinal development and the organotypic retinal explant culture makes this model controversial in postnatal retinal development studies.Thus,we aimed to verify the feasibility of using this model for postnatal retinal development studies by comparing it with the in vivo retina.In this study,we showed that postnatal retinal explants undergo normal development,and exhibit a consistent structure and timeline with retinas in vivo.Initially,we used SOX2 and PAX6 immunostaining to identify retinal progenitor cells.We then examined cell proliferation and migration by immunostaining with Ki-67 and doublecortin,respectively.Ki-67-and doublecortin-positive cells decreased in both in vivo and explants during postnatal retinogenesis,and exhibited a high degree of similarity in abundance and distribution between groups.Additionally,we used Ceh-10 homeodomain-containing homolog,glutamate-ammonia ligase(glutamine synthetase),neuronal nuclei,and ionized calcium-binding adapter molecule 1 immunostaining to examine the emergence of bipolar cells,Müller glia,mature neurons,and microglia,respectively.The timing and spatial patterns of the emergence of these cell types were remarkably consistent between in vivo and explant retinas.Our study showed that the organotypic retinal explant culture model had a high degree of consistency with the progression of in vivo early postnatal retina development.The findings confirm the accuracy and credibility of this model and support its use for long-term,systematic,and continuous observation.展开更多
Burundi, a landlocked country locked near the equator, is one of the least developed countries confirmed by the United Nations and the 60% financial revenue needs aid. Rice is the most important and extravagant food i...Burundi, a landlocked country locked near the equator, is one of the least developed countries confirmed by the United Nations and the 60% financial revenue needs aid. Rice is the most important and extravagant food in Burundian consumption for no more than 5 kg on average per people by year. It's especially suitable to plant rice in Burundi because of the sufficient sunlight and rain. But the lack of new varieties, practical technology and modern service make it very difficult for rice production. According to this significant roll, the rice production has to be increased in order to satisfy the consumption needs which are now becoming higher and keep the food safe. This article mainly shows the rice ecological types, production facts and the major constraints of rice production in Burundi, and then look into the distance on how to solve the problems.展开更多
The study introduced protected agriculture development in Qinghai province and concluded its development scale, type and structure, as well as technology supports. Besides, existing problems were proposed such as lowe...The study introduced protected agriculture development in Qinghai province and concluded its development scale, type and structure, as well as technology supports. Besides, existing problems were proposed such as lower production-level, poor equipment, fewer specialized bases, and countermeasures include the following: leading protected agriculture development by macro-control; strengthening market supervision; improving scientific research and innovation to help S & T achievement transformation.展开更多
[Objective] This paper analyzed present status of broomcorn millet produc- tion in China, including cultivation methods, fertilization utilization, diseases, insect pests and disasters, machinery development, planting...[Objective] This paper analyzed present status of broomcorn millet produc- tion in China, including cultivation methods, fertilization utilization, diseases, insect pests and disasters, machinery development, planting benefit and other aspects. [Method] The corn millet data reported by China Industrial and Technological Re- search System of Millet in 2011 were analyzed by descriptive statistical analysis of EXCEL. [Result] The results show that 17 678.57 kg/ha of farm manure, 155.77 kg/ha of urea, 259.5 kg/ha of compound fertilizer, 203.13 kg/ha of phosphate fertilizer and 75 kg/ha of potash fertilizer were used in China; the national mechanization degree of broomcorn millet production is lower than 30% in all; the average income of broomcorn millet production is 10 503.33 yuan/ha, and the VCR is 2.64; most broomcorn millet producing areas are affected by mild pests and diseases, very few are affected by mild and moderate pests and diseases, and the impact of natural disasters is the lightest. [~onclusien] Currently fertilizer utilization for broomcorn millet is proper but the mechanization level and planting benefit are low, natural disasters, pests and diseases are not severe; finally the authors proposed some suggestions on the fertilizer utilization, mechanization production, benefit increasing, disaster warning, disease and insect controlling and government policy-making for broomcorn millet production.展开更多
This paper summarized the theory and research progress of urban wetland park and elaborated development status of urban wetland park construction at home and abroad,analyzed the existing problems of domestic urban wet...This paper summarized the theory and research progress of urban wetland park and elaborated development status of urban wetland park construction at home and abroad,analyzed the existing problems of domestic urban wetland park.In 1970s,some developed countries began to focus on restoration and construction of ecological environment in wetland and restoration work of habitats,with the sustainable development as the goal,a complete theoretical system was formed initially.But domestic theory research of wetland was relatively weak,and wetland restoration and wetland protection policies,etc.are relatively backward.This paper elaborated current situation of construction and development of urban wetland park,analyzed the existing problems of domestic urban wetland park,which mainly showed in the following aspects:blindly following suit without full scientific verification,unreasonable selecting of lands,unreasonable management system,inadequate policy and regulations,and lack of a huge human resources and mature technologies.This paper also put forward several guidance recommendations for existed problems of domestic urban wetlands from the ecological point of view,including giving primary importance to the protection of ecological environment of water in urban wetland park,and treating fouling species correctly.展开更多
[Objective] This paper presented the current status of mung bean produc- tion in Chongqing, and put forward the corresponding development strategies. [Meth- ods] By overviewing the status of mung bean production in Ch...[Objective] This paper presented the current status of mung bean produc- tion in Chongqing, and put forward the corresponding development strategies. [Meth- ods] By overviewing the status of mung bean production in Chongqing Municipality, the main problems, advantages of mung bean production in Chongqing were pre- sented, and based on corresponding development strategies were put forward.[Re- suits] Currently, the main problems of mung bean production in Chongqing area in- clude insufficient attention, serious variety degradation, lag in cultivation technology, low scientific research level. However, the region has strengths in growing mung beans which would have broad prospects by appropriate adjustment and planning. [Conclusion] This study laid the foundation for the development of mung bean pro- duction and promotion of economic development in Chongqing Municipality.展开更多
Ecotourism development can not only bring economic,ecological and environmental benefits,but also help realize the sustainable development of tourism industry. The authors analyzed the current ecotourism development o...Ecotourism development can not only bring economic,ecological and environmental benefits,but also help realize the sustainable development of tourism industry. The authors analyzed the current ecotourism development of Chaozhou City and existing problems,tried to propose corresponding strategies for its healthy development from legislative and scientific perspectives.展开更多
Based on status quo and existing problems of Hunan crop science and technology innovation, the research proposed "three orientations", including seeking, grasping and highlighting orientations, and "six projects" ...Based on status quo and existing problems of Hunan crop science and technology innovation, the research proposed "three orientations", including seeking, grasping and highlighting orientations, and "six projects" for solving the issues. It is recommended to improve multiple cropping index.展开更多
The Chinese family farm is the most important new micro subject in the construction and development of modern agriculture in China. The investigation and analysis of the actual situation of Hubei Province showed that ...The Chinese family farm is the most important new micro subject in the construction and development of modern agriculture in China. The investigation and analysis of the actual situation of Hubei Province showed that since the implementation of the household contract management system in rural areas, the family farm has followed the track of gradual development, breakthrough development and normal standardization development. In order to promote the family farm to develop rapidly, orderly and healthily, work must be well done to land circulation, scale management, financial support, financial subsidy, cultivation of leading talents and professional farmers, and providing all-round services.展开更多
This paper reviews the development of aquatic vegetable production and research in Hunan. The advantages of aquatic vegetable industry in Hunan are discussed in detail on its location, brand, market, planting and proc...This paper reviews the development of aquatic vegetable production and research in Hunan. The advantages of aquatic vegetable industry in Hunan are discussed in detail on its location, brand, market, planting and processing. And the development strategies of the above-mentioned industry are further analyzed in the paper, such as variety resources, breeding and industrialization.展开更多
Current status and existing problems in the Chinese cosmetic industry areanalyzed from the aspects of industry scale, technology and management level, R&D investment andhuman resources. Strategies to promote the i...Current status and existing problems in the Chinese cosmetic industry areanalyzed from the aspects of industry scale, technology and management level, R&D investment andhuman resources. Strategies to promote the industry are also put forward, such as increasing entrybarrier to optimize industry structure, tightening regulatory controls to improve quality assurance,fostering innovation and actively exploring modes of collaboration, and training more technical andmanaging professionals.展开更多
文摘Farmers' professional cooperatives develop in various forms,and with the continuous improvement of development level,social effects have gradually appear.Based on the specific situation of farmers' professional cooperatives in Shangqiu City,this paper expounded the development status of farmers' professional cooperatives in Shangqiu City,analyzed and discussed various problems encountered in the development process of farmers' professional cooperatives,and put forward some countermeasures and suggestions,such as establishing and perfecting cooperative rules and regulations,financing from multiple channels,introducing technical talents in various ways,breaking the information asymmetry restriction by various means,and giving full play to government functions,aiming to further promote standardized,large-scale,healthy and sustainable development of farmers' professional cooperatives.
基金the financial support from the Scientific Research and Technology Development Project of China Energy Engineering Corporation Limited(CEEC-KJZX-04).
文摘Utilizing energy storage in depleted oil and gas reservoirs can improve productivity while reducing power costs and is one of the best ways to achieve synergistic development of"Carbon Peak–Carbon Neutral"and"Underground Resource Utiliza-tion".Starting from the development of Compressed Air Energy Storage(CAES)technology,the site selection of CAES in depleted gas and oil reservoirs,the evolution mechanism of reservoir dynamic sealing,and the high-flow CAES and injection technology are summarized.It focuses on analyzing the characteristics,key equipment,reservoir construction,application scenarios and cost analysis of CAES projects,and sorting out the technical key points and existing difficulties.The devel-opment trend of CAES technology is proposed,and the future development path is scrutinized to provide reference for the research of CAES projects in depleted oil and gas reservoirs.
基金Supported by Research Project on Basic Education in Jiangxi Province(SZUNDZH2021-1136,SZUNDZH2020-1138).
文摘In recent years,with the support and promotion of relevant policies such as the Opinions on Promoting Study Tour for Primary and Secondary School Students jointly issued by the Ministry of Education and 11 other departments,a wave of study tour has been sparked nationwide from individual pilot projects to gradual promotion.In this paper,students and teachers from grades 4 to 6 of L Primary School in Nanchang City,Jiangxi Province were selected as the survey objects.Combining the survey questionnaire,the current situation of conducting study tour in the primary school was sorted out and analyzed in depth from several aspects such as study tour cognition,study tour participation,study tour implementation,etc.Based on the survey results and relevant research,the current problems in conducting study tours in primary schools were summarized,and targeted improvement measures were proposed,in order to promote the healthy development of study tours in primary schools.
文摘Economies that have effectively escaped the“middle-income trap”demonstrate common traits in their industrial restructuring as they progressed to high-income status.These include a relatively stable share of an economy’s manufacturing sector,a reasonable economic structure,enhanced industrial capabilities,and growth driven by innovation.However,late-moving countries face a number of hurdles as they strive to cross this threshold.China’s development advantages include,among other things,a complete industrial system,a more balanced industrial structure,growing indigenous innovation capabilities,continual expansion and upgrading of domestic demand,and a greater degree of openness.These capabilities have provided continuous momentum for industrial growth,allowing China to capitalize on the next wave of technological and industrial revolutions while also promoting long-term,steady industrial development.During its modernization efforts,China has seen substantial changes in the external environment surrounding its industrial development.We must not only recognize the increasing complexity,intensity,and uncertainty of these changes,but also take proactive steps to solve diverse issues and capitalize on opportunities arising from global digital and green transitions.Equal focus should be placed on strengthening reforms and promoting high-level openness,improving policy coordination and consistency,and pursuing an innovation-driven strategy.This will speed the development of a modern industrial system and encourage the formation of new,high-quality productive forces.
基金Supported by the National Sustainable Development Agenda Innovation Demon-stration Zone in Chengde(202202F009).
文摘The efficacy,application and compatibility of five kinds of Chinese Herbal Medicines medicine(including yam,licorice,Platyc-odonisi Radix,Polygonati Rhizoma,and Pueraria Lobata)for medicine and food homology in the past five years were systematically searched and summarized,and the current situation of their food development was reviewed to provide theoretical basis for the research and development of this kind of CHMs for health care.
基金supported by the National Bulk Freshwater Fish Industry Technology System(CARS-45-33)Tianjin Freshwater Aquaculture Industry Technology System Innovation Team(ITTFRS20211000-005/002)+2 种基金Tianjin Science and Technology Plan Project(23YDTPJC00420)Central Guiding Local Science and Technology Development Fund(24ZYCGSN00250)Open Fund of Key Laboratory of Environmental Standards and Environmental Pollution of the Ministry of Education(202007,202105).
文摘According to the"National Modern Facility Agriculture Construction Plan(2023-2030)released by the Ministry of Agriculture and Rural Affairs,the"Special Implementation Plan for Modern Facility Fisheries Construction"emphasizes the development of circular aquaculture,active expansion of green aquaculture space in facility fisheries,multi-channel development of food sources,comprehensive improvement of the quality,efficiency,and competitiveness of facility fisheries,ensuring stable and safe supply of aquatic products,and better meeting people's needs for high-quality aquatic products and beautiful aquatic ecological environment.Since the implementation of the 14th Five Year Plan for fisheries,facility fisheries have made significant progress.In order to thoroughly implement General Secretary Xi Jinping's important discourse on new quality productivity,conduct in-depth research on the current situation and existing problems of the fishery industry,and put forward suggestions to meet the requirements of high-quality development of the fishery industry in the new era.Therefore,it is crucial to continue practicing the"revitalization of aquatic seed industry"and"big food concept",take multiple measures to"demand food from rivers,lakes,and seas",and expand aquaculture space.Through research,it has been found that research in the field of facility based fisheries is still in its developmental stage.Further exploration is needed on the current status,breeding models,and experimental demonstrations of land-based facility based fisheries,in order to provide a basis for fisheries regulatory authorities to formulate the"15th Five Year Plan"for facility based fisheries.
基金supported by the Japan Society for the Promotion of Science KAKENHI(grant Nos.23K05678 to IM,19H05711 and 20H00466 to KS)the Joint Research Program of Institute for Molecular and Cellular Regulation,Gunma University(to KS)。
文摘In eukaryotic cells,organelles in the secretory,lysosomal,and endocytic pathways actively exchange biological materials with each other through intracellular membrane trafficking,which is the process of transporting the cargo of proteins,lipids,and other molecules to appropriate compartments via transport vesicles or intermediates.These processes are strictly regulated by various small GTPases such as the RAS-like in rat brain(RAB)protein family,which is the largest subfamily of the RAS superfamily.Dysfunction of membrane trafficking affects tissue homeostasis and leads to a wide range of diseases,including neurological disorders and neurodegenerative diseases.Therefore,it is important to understand the physiological and pathological roles of RAB proteins in brain function.RAB35,a member of the RAB family,is an evolutionarily conserved protein in metazoans.A wide range of studies using cultured mammalian cells and model organisms have revealed that RAB35 mediates various processes such as cytokinesis,endocytic recycling,actin bundling,and cell migration.RAB35 is also involved in neurite outgrowth and turnover of synaptic vesicles.We generated brain-specific Rab35 knockout mice to study the physiological roles of RAB35 in brain development and function.These mice exhibited defects in anxiety-related behaviors and spatial memory.Strikingly,RAB35 is required for the precise positioning of pyramidal neurons during hippocampal development,and thereby for normal hippocampal lamination.In contrast,layer formation in the cerebral cortex occurred superficially,even in the absence of RAB35,suggesting a predominant role for RAB35 in hippocampal development rather than in cerebral cortex development.Recent studies have suggested an association between RAB35 and neurodegenerative diseases,including Parkinson's disease and Alzheimer's disease.In this review,we provide an overview of the current understanding of subcellular functions of RAB35.We also provide insights into the physiological role of RAB35 in mammalian brain development and function,and discuss the involvement of RAB35 dysfunction in neurodegenerative diseases.
基金This article is the research result of the Research and Practice Project on Education and Teaching Reform at Shandong University of Science and Technology Jinan Campus:"Research on the Blended Teaching Model of Online Courses and Full English Classroom Teaching"(JNJG202101),and 2021 Curriculum Special Construction Project(JNKCZX2021109)of Shandong University of Science and Technology Jinan Campus.
文摘Faced with unprecedented changes in the world,China has chosen to continue expanding its opening-up and actively cooperate with other countries.Internationalization of education is one of the important directions for the future development of universities.Since the reform and opening-up,a series of Sino-foreign cooperative education policies have been successively released,forming a relatively systematic and complete policy system.Under the promotion of policies,Chinese universities actively engage in Sino-foreign cooperative education,with increasingly frequent educational exchanges and a clear trend towards internationalization.The scale of Sino-foreign cooperative education is also constantly expanding.It is worthy to explore how to draw on advanced teaching experience from overseas,continue to promote Sino-foreign cooperative education,and improve the quality of Sino-foreign cooperative education.
基金supported by the National Key R&D Program of China,No.2019YFA0110300(to ZG)the National Natural Science Foundation of China,Nos.81773302(to YF),32070862(to ZG).
文摘Human brain development is a complex process,and animal models often have significant limitations.To address this,researchers have developed pluripotent stem cell-derived three-dimensional structures,known as brain-like organoids,to more accurately model early human brain development and disease.To enable more consistent and intuitive reproduction of early brain development,in this study,we incorporated forebrain organoid culture technology into the traditional unguided method of brain organoid culture.This involved embedding organoids in matrigel for only 7 days during the rapid expansion phase of the neural epithelium and then removing them from the matrigel for further cultivation,resulting in a new type of human brain organoid system.This cerebral organoid system replicated the temporospatial characteristics of early human brain development,including neuroepithelium derivation,neural progenitor cell production and maintenance,neuron differentiation and migration,and cortical layer patterning and formation,providing more consistent and reproducible organoids for developmental modeling and toxicology testing.As a proof of concept,we applied the heavy metal cadmium to this newly improved organoid system to test whether it could be used to evaluate the neurotoxicity of environmental toxins.Brain organoids exposed to cadmium for 7 or 14 days manifested severe damage and abnormalities in their neurodevelopmental patterns,including bursts of cortical cell death and premature differentiation.Cadmium exposure caused progressive depletion of neural progenitor cells and loss of organoid integrity,accompanied by compensatory cell proliferation at ectopic locations.The convenience,flexibility,and controllability of this newly developed organoid platform make it a powerful and affordable alternative to animal models for use in neurodevelopmental,neurological,and neurotoxicological studies.
基金supported by the National Natural Science Foundation of China,Nos.81901156(to ZZ),82271200(to ZZ),82171308(to XC)the Fundamental Research Funds for the Central Universities,No.xzy012022035(to ZZ)+1 种基金the Natural Science Foundation of Shaanxi Province,Nos.2021JM-261(to QK),2023-YBSF-303(to ZZ)Traditional Chinese Medicine Project of Shaanxi Province,No.2019-ZZ-JC047(to QK)。
文摘The organotypic retinal explant culture has been established for more than a decade and offers a range of unique advantages compared with in vivo experiments and cell cultures.However,the lack of systematic and continuous comparison between in vivo retinal development and the organotypic retinal explant culture makes this model controversial in postnatal retinal development studies.Thus,we aimed to verify the feasibility of using this model for postnatal retinal development studies by comparing it with the in vivo retina.In this study,we showed that postnatal retinal explants undergo normal development,and exhibit a consistent structure and timeline with retinas in vivo.Initially,we used SOX2 and PAX6 immunostaining to identify retinal progenitor cells.We then examined cell proliferation and migration by immunostaining with Ki-67 and doublecortin,respectively.Ki-67-and doublecortin-positive cells decreased in both in vivo and explants during postnatal retinogenesis,and exhibited a high degree of similarity in abundance and distribution between groups.Additionally,we used Ceh-10 homeodomain-containing homolog,glutamate-ammonia ligase(glutamine synthetase),neuronal nuclei,and ionized calcium-binding adapter molecule 1 immunostaining to examine the emergence of bipolar cells,Müller glia,mature neurons,and microglia,respectively.The timing and spatial patterns of the emergence of these cell types were remarkably consistent between in vivo and explant retinas.Our study showed that the organotypic retinal explant culture model had a high degree of consistency with the progression of in vivo early postnatal retina development.The findings confirm the accuracy and credibility of this model and support its use for long-term,systematic,and continuous observation.
基金The Aid Project on Science and Technology to the Developing Countries from the Ministry of Science and Technology of the PRC:Research Cooperation for key technologies to improve food production in Africa,Guangxi Scientific Research and Technology Development Project(No.10100020-1,1346009-4,14125007-2-7)Fundamental Research Funds for the GXAAS(2014YZ38)~~
文摘Burundi, a landlocked country locked near the equator, is one of the least developed countries confirmed by the United Nations and the 60% financial revenue needs aid. Rice is the most important and extravagant food in Burundian consumption for no more than 5 kg on average per people by year. It's especially suitable to plant rice in Burundi because of the sufficient sunlight and rain. But the lack of new varieties, practical technology and modern service make it very difficult for rice production. According to this significant roll, the rice production has to be increased in order to satisfy the consumption needs which are now becoming higher and keep the food safe. This article mainly shows the rice ecological types, production facts and the major constraints of rice production in Burundi, and then look into the distance on how to solve the problems.
文摘The study introduced protected agriculture development in Qinghai province and concluded its development scale, type and structure, as well as technology supports. Besides, existing problems were proposed such as lower production-level, poor equipment, fewer specialized bases, and countermeasures include the following: leading protected agriculture development by macro-control; strengthening market supervision; improving scientific research and innovation to help S & T achievement transformation.
基金Supported by Special Fund for Construction of Modern Agricultural Technology System(CARS-07-12.5-A18)~~
文摘[Objective] This paper analyzed present status of broomcorn millet produc- tion in China, including cultivation methods, fertilization utilization, diseases, insect pests and disasters, machinery development, planting benefit and other aspects. [Method] The corn millet data reported by China Industrial and Technological Re- search System of Millet in 2011 were analyzed by descriptive statistical analysis of EXCEL. [Result] The results show that 17 678.57 kg/ha of farm manure, 155.77 kg/ha of urea, 259.5 kg/ha of compound fertilizer, 203.13 kg/ha of phosphate fertilizer and 75 kg/ha of potash fertilizer were used in China; the national mechanization degree of broomcorn millet production is lower than 30% in all; the average income of broomcorn millet production is 10 503.33 yuan/ha, and the VCR is 2.64; most broomcorn millet producing areas are affected by mild pests and diseases, very few are affected by mild and moderate pests and diseases, and the impact of natural disasters is the lightest. [~onclusien] Currently fertilizer utilization for broomcorn millet is proper but the mechanization level and planting benefit are low, natural disasters, pests and diseases are not severe; finally the authors proposed some suggestions on the fertilizer utilization, mechanization production, benefit increasing, disaster warning, disease and insect controlling and government policy-making for broomcorn millet production.
基金Supported by Research Project of Jiangxi Provincial Office of Education(GJJ10403)~~
文摘This paper summarized the theory and research progress of urban wetland park and elaborated development status of urban wetland park construction at home and abroad,analyzed the existing problems of domestic urban wetland park.In 1970s,some developed countries began to focus on restoration and construction of ecological environment in wetland and restoration work of habitats,with the sustainable development as the goal,a complete theoretical system was formed initially.But domestic theory research of wetland was relatively weak,and wetland restoration and wetland protection policies,etc.are relatively backward.This paper elaborated current situation of construction and development of urban wetland park,analyzed the existing problems of domestic urban wetland park,which mainly showed in the following aspects:blindly following suit without full scientific verification,unreasonable selecting of lands,unreasonable management system,inadequate policy and regulations,and lack of a huge human resources and mature technologies.This paper also put forward several guidance recommendations for existed problems of domestic urban wetlands from the ecological point of view,including giving primary importance to the protection of ecological environment of water in urban wetland park,and treating fouling species correctly.
基金Supported by the China Agriculture Research System(CARS-09)the Fund for the Construction of Scientific and Technological Innovation Capacity of Chongqing Municipality(CSTC,2009CB1007)the Development Fund for Agriculture of Chongqing Municipality~~
文摘[Objective] This paper presented the current status of mung bean produc- tion in Chongqing, and put forward the corresponding development strategies. [Meth- ods] By overviewing the status of mung bean production in Chongqing Municipality, the main problems, advantages of mung bean production in Chongqing were pre- sented, and based on corresponding development strategies were put forward.[Re- suits] Currently, the main problems of mung bean production in Chongqing area in- clude insufficient attention, serious variety degradation, lag in cultivation technology, low scientific research level. However, the region has strengths in growing mung beans which would have broad prospects by appropriate adjustment and planning. [Conclusion] This study laid the foundation for the development of mung bean pro- duction and promotion of economic development in Chongqing Municipality.
基金Sponsored by Guangdong Provincial Philosophical and Social Science Program(090-02)~~
文摘Ecotourism development can not only bring economic,ecological and environmental benefits,but also help realize the sustainable development of tourism industry. The authors analyzed the current ecotourism development of Chaozhou City and existing problems,tried to propose corresponding strategies for its healthy development from legislative and scientific perspectives.
基金Supported by Science&Technology Innovation Project of Hunan Academy of Agricultural Sciences~~
文摘Based on status quo and existing problems of Hunan crop science and technology innovation, the research proposed "three orientations", including seeking, grasping and highlighting orientations, and "six projects" for solving the issues. It is recommended to improve multiple cropping index.
基金Supported by the Social Science Funding Project of Hubei Province(201590)~~
文摘The Chinese family farm is the most important new micro subject in the construction and development of modern agriculture in China. The investigation and analysis of the actual situation of Hubei Province showed that since the implementation of the household contract management system in rural areas, the family farm has followed the track of gradual development, breakthrough development and normal standardization development. In order to promote the family farm to develop rapidly, orderly and healthily, work must be well done to land circulation, scale management, financial support, financial subsidy, cultivation of leading talents and professional farmers, and providing all-round services.
基金Supported by Special Fund for Constructing Innovation-oriented City(K1307028-31)Special Poverty Relief Program of Scientific Special Commissioners in Hunan Province~~
文摘This paper reviews the development of aquatic vegetable production and research in Hunan. The advantages of aquatic vegetable industry in Hunan are discussed in detail on its location, brand, market, planting and processing. And the development strategies of the above-mentioned industry are further analyzed in the paper, such as variety resources, breeding and industrialization.
文摘Current status and existing problems in the Chinese cosmetic industry areanalyzed from the aspects of industry scale, technology and management level, R&D investment andhuman resources. Strategies to promote the industry are also put forward, such as increasing entrybarrier to optimize industry structure, tightening regulatory controls to improve quality assurance,fostering innovation and actively exploring modes of collaboration, and training more technical andmanaging professionals.