New aspects of a small GTPase RAB35 in brain development and function

In eukaryotic cells,organelles in the secretory,lysosomal,and endocytic pathways actively exchange biological materials with each other through intracellular membrane trafficking,which is the process of transporting the cargo of proteins,lipids,and other molecules to appropriate compartments via transport vesicles or intermediates.These processes are strictly regulated by various small GTPases such as the RAS-like in rat brain(RAB)protein family,which is the largest subfamily of the RAS superfamily.Dysfunction of membrane trafficking affects tissue homeostasis and leads to a wide range of diseases,including neurological disorders and neurodegenerative diseases.Therefore,it is important to understand the physiological and pathological roles of RAB proteins in brain function.RAB35,a member of the RAB family,is an evolutionarily conserved protein in metazoans.A wide range of studies using cultured mammalian cells and model organisms have revealed that RAB35 mediates various processes such as cytokinesis,endocytic recycling,actin bundling,and cell migration.RAB35 is also involved in neurite outgrowth and turnover of synaptic vesicles.We generated brain-specific Rab35 knockout mice to study the physiological roles of RAB35 in brain development and function.These mice exhibited defects in anxiety-related behaviors and spatial memory.Strikingly,RAB35 is required for the precise positioning of pyramidal neurons during hippocampal development,and thereby for normal hippocampal lamination.In contrast,layer formation in the cerebral cortex occurred superficially,even in the absence of RAB35,suggesting a predominant role for RAB35 in hippocampal development rather than in cerebral cortex development.Recent studies have suggested an association between RAB35 and neurodegenerative diseases,including Parkinson's disease and Alzheimer's disease.In this review,we provide an overview of the current understanding of subcellular functions of RAB35.We also provide insights into the physiological role of RAB35 in mammalian brain development and function,and discuss the involvement of RAB35 dysfunction in neurodegenerative diseases.

Establishment of human cerebral organoid systems to model early neural development and assess the central neurotoxicity of environmental toxins

Human brain development is a complex process,and animal models often have significant limitations.To address this,researchers have developed pluripotent stem cell-derived three-dimensional structures,known as brain-like organoids,to more accurately model early human brain development and disease.To enable more consistent and intuitive reproduction of early brain development,in this study,we incorporated forebrain organoid culture technology into the traditional unguided method of brain organoid culture.This involved embedding organoids in matrigel for only 7 days during the rapid expansion phase of the neural epithelium and then removing them from the matrigel for further cultivation,resulting in a new type of human brain organoid system.This cerebral organoid system replicated the temporospatial characteristics of early human brain development,including neuroepithelium derivation,neural progenitor cell production and maintenance,neuron differentiation and migration,and cortical layer patterning and formation,providing more consistent and reproducible organoids for developmental modeling and toxicology testing.As a proof of concept,we applied the heavy metal cadmium to this newly improved organoid system to test whether it could be used to evaluate the neurotoxicity of environmental toxins.Brain organoids exposed to cadmium for 7 or 14 days manifested severe damage and abnormalities in their neurodevelopmental patterns,including bursts of cortical cell death and premature differentiation.Cadmium exposure caused progressive depletion of neural progenitor cells and loss of organoid integrity,accompanied by compensatory cell proliferation at ectopic locations.The convenience,flexibility,and controllability of this newly developed organoid platform make it a powerful and affordable alternative to animal models for use in neurodevelopmental,neurological,and neurotoxicological studies.

Postnatal development of rat retina:a continuous observation and comparison between the organotypic retinal explant model and in vivo development

The organotypic retinal explant culture has been established for more than a decade and offers a range of unique advantages compared with in vivo experiments and cell cultures.However,the lack of systematic and continuous comparison between in vivo retinal development and the organotypic retinal explant culture makes this model controversial in postnatal retinal development studies.Thus,we aimed to verify the feasibility of using this model for postnatal retinal development studies by comparing it with the in vivo retina.In this study,we showed that postnatal retinal explants undergo normal development,and exhibit a consistent structure and timeline with retinas in vivo.Initially,we used SOX2 and PAX6 immunostaining to identify retinal progenitor cells.We then examined cell proliferation and migration by immunostaining with Ki-67 and doublecortin,respectively.Ki-67-and doublecortin-positive cells decreased in both in vivo and explants during postnatal retinogenesis,and exhibited a high degree of similarity in abundance and distribution between groups.Additionally,we used Ceh-10 homeodomain-containing homolog,glutamate-ammonia ligase(glutamine synthetase),neuronal nuclei,and ionized calcium-binding adapter molecule 1 immunostaining to examine the emergence of bipolar cells,Müller glia,mature neurons,and microglia,respectively.The timing and spatial patterns of the emergence of these cell types were remarkably consistent between in vivo and explant retinas.Our study showed that the organotypic retinal explant culture model had a high degree of consistency with the progression of in vivo early postnatal retina development.The findings confirm the accuracy and credibility of this model and support its use for long-term,systematic,and continuous observation.

Effect of coenzyme Q10 supplementation on post-vitrification mouse embryo development

Objective:To investigate the effects of coenzyme Q10(CoQ10)supplementation on post-vitrification embryo development and gross morphology.Methods:Balb/c mouse embryos were cultured in potassium simplex optimised medium(KSOM)with varying CoQ10 concentrations[0(control),20,40,and 60μM].The most effective CoQ10 concentration(40μM)was selected for subsequent post-vitrification morphology study.Embryos were randomly divided into four groups:Group A(non-vitrified without CoQ10),Group B(non-vitrified with CoQ10),Group C(vitrified without CoQ10),and Group D(vitrified with CoQ10),followed by vitrification at the 8-cell stage.Survival rates and development until the blastocyst stage were evaluated through morphological examinations using ASEBIR's system,distinguishing normal and abnormal embryos.Results:Supplementation of 40μM CoQ10 significantly increased blastocyst formation(95%)compared to the control group(92%),20μM(62%),and 60μM(56%)(P<0.001).Following vitrification,Group D exhibited a significant increase in blastocyst formation(92%)compared to Group C(82%)(P<0.05).Morphological assessments indicated superior embryo quality in Group B over Group D during the cleavage stage,morula,and blastocyst(P<0.05).Conclusions:CoQ10 supplementation exhibits promising potential to enhance preimplantation embryo development,increase blastocyst formation rates,and improve embryo quality post-vitrification.This offers a promising approach to mitigate oxidative stress on embryos,potentially improving overall assisted reproductive technology outcomes.

Analysis on the Development Model of Relic and Museum Tourism Products Based on ERP Analysis:A Case Study on Shandong Province

In recent years, the trend of people choosing relic and museum tourism during their vacationand leisure time is growing day by day, mainly because it touches people's feelings about historical relicsand cultural heritage. The development of relic and museum tourism products is not only the inheritanceof culture and the protection of cultural relics, but also the promotion of tourism consumption under thebackground of cultural tourism integration, which has promoted the development of regional economy.With the relic and museum tourism resources in Shandong Province as the research object, through theanalysis of its resources, market and products, this paper put forward three applicable modes of relic andmuseum tourism product development, including independent development, joint development, and artauthorization, and proposed specific suggestions around the three modes.

Development Strategy of Sichuan Sports Museum under the Background of Cultural and Tourism Integration

Through conducting a SWOT analysis of the Chengdu Sports Institute Museum,and utilizing the 4M management,5W display,and 4P marketing theoretical models,this study explores effective strategies suitable for Sichuan sports museums in terms of functionality,display,and marketing.

Research on the Constitutional,Historical and Practical Logic of the Chinese Path of Human Rights Development——From the Perspective of the Inclusion of Human Rights Clause in the Constitution

The inclusion of the human rights clause in the Chi-nese Constitution is a concern and expression of the spirit of the Con-stitution,which has laid the institutional regulations of the fundamen-tal law for the development of human rights in China,provided the principles and value norms of the highest level of effectiveness for the legal protection of human rights,and built a profound constitutional basis for the formation and improvement of the Chinese path of human rights development.The human rights clause is not only a summariza-tion and affirmation of the historical practice of Chinese human rights development under the leadership of the Communist Party of China,but also a new starting point for the development of human rights in China under the leadership of the Communist Party of China.It marks that the development of human rights in China has entered a new era.The Party and the state have finally embarked on a path of human rights development with Chinese characteristics by formulating and implementing the Human Rights Action Plan of China,eliminating ab-solute poverty through the national poverty alleviation campaign,and promoting human rights protection through the rule of law.

Discussion on Green Development of Fenlong for Yield Increase, Quality Enhancing, Water Retaining and Multiple Use of Natural Resources

Fenlong green ecological agriculture technology （Fenlong technology）, a new smash ridging farming method developed by Guangxi Academy of Agdcultural Sciences, has been elected as the recommended cultivation technique by the Ministry of Agriculture of China. It replaces the traditional plowshare with spiral drill, and its tilth depth is twice deeper than that by tractor tilthing. It also extends soil nutrient, moisture, oxygen and microorganism, the so-called ＂Four pools＂. Soil nutrient, oxygen, microorganism, light and rainfall use ratio is increased by 10%-100%, creating a platform for natural increase of more than 10% of crop yield. Its application to over 20 kinds of crops in 21 provinces has proved that the yield increases 10-30% with quality enhancing 5% and double water retaining capacity but no more input. When the application area of Fenlong could reach 67 million hm2, the amount of fertilizer can be reduced by 40-50 billion kg, saving 120-150 billion Yuan. In this paper, we put forward the strategy of ＂4＋1＂ （arable, saline-alkali soil, grasslands, Sponge City ＋ rivers） green development in China, and deepened the Fenlong cultivated tilled layer from 16.5 cm to 35 cm for 67 million hm2 arable land, ridged 13.3 million hm2 of saline-alkali soil for 35 cm, and also 35 cm for 67 million hm2 degraded steppe, which could have the following 3 effects： first, the 147 million hm2 of land with Fenlong cultivation could increase loosing soil to 315.491 billion m3, in＊ creasing by 159.26% for 120 million hm2 of arable land with the average tilled layer of 16.5 cm, which has loosing soil of only 198.1 billion m3, that is, the space of the land increases 1.6 times. Second, every hectare of plowland could store up to 450 m3/hm2 of natural rainfall, and the unused 60 m3 of saline-alkali soil and grasslands could store water of 102 billion m3, showing an increase of over 88.89% for the current plowland storage of 54 billion m3 at now, that is, double the natural rainfall storage capacity. Third, the two multiple increase of natural resources application can bring trillions of resource activation, environmental cleaning, food security, citizens, health, economic, ecological and social benefits, and makes the Chinese nation move forward in green development. Its application in ＂big scientific research＂ and ＂One Belt And One Road＂ will contribute Chinese strength to the world.

The Research on Development and Causes of Chinese Quadrangle Courtyards

After the illustration of the development of quadrangle courtyards,the paper has analyzed the emergence of square architectural form and the formation of axial symmetrical pattern with the hall in the front and room at the back and with wing rooms on the left and right side.It has analyzed the formation,development,climax,damage and inheritance of quadrangle courtyard,and considered that it could manifest Chinese traditional humanistic idea and aesthetic idea,and is in possession of profound cultural connotation and regional feature.On this basis,causes for quadrangle courtyards have been studied.The primary cause at the initial stage lies in human body structure theory and the perfection of quadrangle courtyards at later stage depends on ethic order theory.

Current Use and Coordinated Development of Traditional Residential Districts from the Perspective of Human Settlement—A Case Study of Nanyuan Dormitory Area of Anhui Agricultural University

In view of China's rapid urbanization,Nanyuan Dormitory Area of Anhui Agricultural University was studied as a representative of traditional living quarters with harmonious neighborhood relationship to discuss current use and coordinated development of traditional residential districts from the perspective of human settlement.Problems in the dormitory area were analyzed,neighboring environment,regional characteristics,cultures and all relevant elements were fully considered to build a favorable human settlement by following the coordinated development of human settlement and economy.Renovation strategies on the basis of maintaining original favorable human settlement were discussed from the perspectives of space ecology,dwelling environment,facilities and humanistic environment,so as to provide new concepts for the sustainable development of urban traditional residential districts and the construction of favorable human settlement.

Study on Growth and Development and Dry Matter Production of Greenhouse Tomato in North China

[Objective] This study aimed to investigate the growth and development and dry matter production and allocation of greenhouse tomato in North China. [Method] With tomato cultivar ＇Jinguo No.l＇ as the experimental material, the ex- periments were conducted in a solar greenhouse located in Taigu, Shanxi （37o25＇ N, 112o25＇ E） from March to July, in 2014 and 2015. [Result] The plant height, leaf number, leaf area and dry matter production of greenhouse tomato increased lin- early with the increased days after transplantation, and their growth rates were 2.74 cm/d, 0.32 leaf/d, 70 cm2/d and 9.4 g/（m2.d）, respectively. The fruit dry weight also showed a linear relationship with the plant dry weight. After fruit setting, the fruit dry weight was increased by about 0.69 g when the plant dry weight was increased by 1 g. The allocation proportions of dry matter in tomato roots, stems and leaves de- clined significantly from plant flowering to fruiting, while the proportion of fruit dry weight in plant dry weight increased significantly, exceeding 60%. There was a strong linear relationship between dry matter production of tomato and effective cu- mulative temperature （sum of temperature higher than 10 ^（3） in the solar green- house, and the dry matter production was increased by about 0.73 g/m2 when the effective cumulative temperature was increased by one unit. The dry matter produc- tion of tomato plant also showed a strong linear relationship with photosynthetic ac- tive radiation （PAR） in the greenhouse, and about 3.4 g of dry matter was synthe- sized per unit （M J） of PAR. [Conclusion] Extending the growing period and improv- ing the temperature and light conditions could increase the dry matter production of tomato.

The influencing factors and Development Countermeasures of Large Farming Household in Jiangxi Province

As an important part of the new agricultural business entities, large-scale grain-production households play a significantly positive role in improving the land resource utilization, improving agricultural productivity, increasing agricultural output and farmers＂ income, and making a certain contribution to stabilize grain production. This paper analyzed the current situation of large-scale grain-production household in Jiangxi Province, as well as the problems in land transfer, farmland infrastructure, production and management, capital and other risks. At last, the paper proposed targeted countermeasures.

Study on the Further Development of Cultural and Ecological Tourism in Zhenshan Eco-museum

Based on analyzing the existing problems in the development of Zhenshan Eco-museum tourism,this study aimed to plan and design the structure of Zhenshan ethnic cultural and ecological tourism zone,and the projects of cultural and ecological tourism under the premise of protection and development.

Program Development and Implementation of Landscape Pattern Indexes for Land Use Based on RS

Interpretation results of RS images were taken as the foundation, ETM RS images of July 15, 2009 as the data source, ENVI was applied for the preprocessing, Arc GIS used to finish the interpretation, master data obtained, and VB used to complete program development. 12 representative landscape pattern indexes were selected, Excel data used to create Access database, and VB used to complete the program development. Selection of landscape pattern indexes, program development process and program usage were summarized, through comparing its results with Excel calculation results, it was found that calculation results of this program were correct, the usage was convenient, so needs of most users could be satisfied.

Study on the Gene Transcription Level of Hippocampus NGF in C_(57) Mouse Development

β-Nerve growth factor (β NGF) mRNA levels in hippocampa from C57BL/6J mice of different ages were compared by semi-quantitative RT-PCR method, taking β-actin gene as an internal control. The levels of 6-and 12-month-old C57 mice were much lower compared with that of the l-month-old mouse. However, there was no significant difference between these two groups. This result suggests that gene transcription level of hippocampus β NGF in a C57, mouse descends evidently as it grows up. Base T at Site 294 of β NGF cDNA was substituted by C in the C57, mouse, as revealed by DNA sequencing for the first time. Nevertheless, this polymorphism of nucleotide did not make any difference in amino acid composition.

GIS-based Ecological Suitability Assessment for Land Use——A Case Study of an Economic Development Zone in Shenyang

Analysis indices of ecological suitability were selected through literature reviewing and expert consultation. Visualization of assessment course was achieved based on different land use functions with the help of ArcGIS9.0 and GIS layers with different properties were overlapped and computed with GIS grids. Hence, the areas were colored and reclassified（colors representing different ecological suitability degrees）. Finally, land use functions can be readjusted by the evaluation results,providing references for rational planning of economic development zones.

GAMYB transcription factor LoMYB65 from lily plays a vital role in pollen development

Lily(Lilium spp.)is an important horticultural crop,but its use is limited due to serious pollen contamination problems.There are many studies on pollen development in model plants,but few on flower crops such as lilies.Gibberellin(GA)is a large class of hormones and plays an important role in plant vegetative growth and reproductive development.GAMYB is a group of the R2R3-MYB family upregulated by gibberellin,and plays an important role in anther development.Here,we isolated a novel GAMYB,named LoMYB65,from lily,which was closely related to the AtMYB65 and AtMYB33 in Arabidopsis.Fluorescence quantitative PCR results showed that LoMYB65 was mainly expressed in lily anthers.LoMYB65 could be activated by 288μmol·L^(-1)GA3treatment and the LoMYB65 protein was located in the nucleus and cytoplasm,and had transactivation in yeast and tobacco leaf cells.The conserved motif within 226 amino acids of the C-terminal of LoMYB65 contributed to its transactivation.Overexpression of LoMYB65 caused dwarf phenotype,unnormal tapetum development,less seeds of siliques in transgenic Arabidopsis plants,the transgenic plants showed partly male sterile.Simultaneously,silencing of LoMYB65 with VIGS(Virus Induced Gene Silencing)in lily anthers caused unnormal pollen development and reduced the pollen amount.Overexpression of LoMYB65 in Arabidopsis and silencing of LoMYB65 in lily resulted in decreased pollen counts,so we speculate that LoMYB65 may be dose-dependent.Overall,these findings suggest that LoMYB65 may play an important role in anther development and pollen formation in lily.LoMYB65 may provide a useful candidate gene for pollenless breeding of lily.

Brownfield Redevelopment Toward Sustainable Urban Land Use in China

Brownfield redevelopment is a new urban land use strategy to pursue the ideal of sustainable development. It relieves environmental pressure and resolves the glaring contradiction between urban land idleness and urban sprawl. It addresses issues of urban land use and farmland protection in China. This paper compares brownfield with greenfield, and defines brownfield and its redevelopment. It elaborates general and special government policies for this sustainable land use strategy in the context of China. General policies contain two aspects: clarifying brownfield redevelopment's priority in Chinese urban planning and land use and setting a general quantitative redevelopment target. The extent of the government's special policies, which aim at coordinating three factors in the redevelopment process, namely govern- ments, developers and users, are divided into five areas: statistics and categorization, assessment and remediation, fi- nancial supports and tax incentives, environmental and legal liability, and publicity and guidance. The paper concludes the major functions of different levels of Chinese government in the redevelopment process, i.e. making general strategy, formulating legislation, spectifying regulations and technical standards, estimating and categorizing brownfield sites, drawing up brownfield planning, providing information and financial support, guiding developers, publicizing the strategy to the public, and checking reuse results.

Discussion on the Index System of Intensive Land Use Evaluation in Development Area

The establishment of evaluation index system is the key to the evaluation of intensive land use.On the basis of expounding connotation,characteristics and the principle of establishing evaluation index system,and as for the problems existing in Evaluation Regulation(Trial)of Intensive Land Use in Development Area,regarding the evaluation index system of intensive land use in the development area,in the light of the connotation of intensive land use in development area,coupled with the practical situation of the land use in China's development area,after referring to the research achievements of domestic and abroad scholars,some suggestions are put forward in order to improve and perfect the evaluation index system of intensive land us in development area.

Background of Land Development and Opportunity of Land Use Transition

In order to ease the contradiction between people and land and ensure the needs of construction land and farmland,this paper analyzes the demand and regional land development background,and points out the problems of the development of the land in China: late start of land development and utilization practices; declining back-up resources and sharply increasing costs; new resources and environmental problems brought about by development and utilization of land in some areas. This paper presents a new opportunity and strategy for land use transition: giving full play to the agricultural and geographical advantages of farming-pastoral area; carrying out water-saving technologies and intensive use of agricultural land in water-deficient areas,in order to achieve reasonable and efficient development and utilization.