Developmental dependence for functional eye regrowth in Xenopus laevis

Introduction：A key challenge in designing tissue repair strategies is knowing whether and how developmental mechanisms are used for successful repair of mature/adult tissues.Although it is known that developmental components are used in repair,it remains mostly unclear which ones are required and whether they act similarly as during development.This issue is further complicated by the fact that it is difficult.

Changes in hippocampal neurons and memory function during the developmental stage of newborn rats with hypoxic-ischemic encephalopathy

BACKGROUND: Under the normal circumstance, there exist some synapses with inactive functions in central nervous system (CNS), but these functions are activated following nerve injury. At the early stage of brain injury, the abnormal functions of brain are varied, and they have very strong plasticity and are corrected easily. OBJECTIVE: To observe the changes of neuronal morphology in hippocampal CA1 region and memory function in newborn rats with hypoxic-ischemic encephalopathy(HIE) from ischemia 6 hours to adult. DESIGN: Completely randomized grouping, controlled experiment. SETTING: Taian Health Center for Women and Children; Taishan Medical College. MATERIALS: Altogether 120 seven-day-old Wistar rats, of clean grade, were provided by the Experimental Animal Center, Shandong University of Traditional Chinese Medicine. Synaptophysin (SYN) polyclonal antibody was provided by Maixin Biological Company, Fuzhou. METHODS: This experiment was carried out in the Laboratory of Morphology, Taishan Medical College between October 2000 and December 2003. ① The newborn rats were randomly divided into 2 groups: model group and control group, 60 rats in each group. Five rats were chosen from each group at postoperative 6 hours, 24 hours, 72 hours, 7 days, 2 weeks and 3 weeks separately for immunohistochemical staining. Fifteen newborn rats were chosen from each group at postoperative 4 weeks and 2 months separately for testing memory ability (After test, 5 rats from each group were sacrificed and used for immunohistochemical staining)② The right common carotid artery of newborn rats of model group was ligated under the anesthetized status. After two hours of incubation, the rats were placed for 2 hours in a container filled with nitrogen oxygen atmosphere containing 0.08 volume fraction of oxygen, thus, HIE models were created; As for the newborn rats in the control group, only blood vessels were isolated, and they were not ligated and hypoxia-treated. ③ Thalamencephal tissue sections of newborn rats of two groups were performed DAB developing and haematoxylin slight staining. Cells with normal nucleous in 250 μm-long granular layer which started from hippocampal CA1 region were counted with image analysis system under high-fold optical microscope (×600), and the thickness of granular layer was measured. The absorbance (A) of positive reactant of SYN in immunohistochemically-stained CA1 region was measured. Learning and memory ability were measured with step through test 3 times successively. ④ t test and paired t test were used for comparing intergroup and intragroup difference of measurement data respectively, and Chi-square for comparing the difference of enumeration data. MAIN OUTCOME MEASURES: Comparison of cytological changes in hippocampal CA1 region and memory ability at different postoperative time points between two groups. RESULTS: Totally 120 newborn rats were involved in the result analysis. ① Cell morphological changes in hippocampal CA1 region: In the control group, with aging, perikaryon, nucleus and nucleolus in cortex of parietal lobe were significantly increased, Nissl body was compacted, the amount of neurons was declined, but the A of SYN positive reactant was relatively increased. In the model group, at postoperative each time point, neurons were seriously shrunk and dark-stained, nucleus was contracted, chromatin was condensed, nucleolus was unclear, even cells disappeared, especially the cells in 6 hours and 24 hours groups. The amount of neurons with normal morphology in hippocampal CA1 region and granular layer thickness in the model group at postoperative each time point were significantly less or smaller than those in the control group at postoperative 6 hours respectively (t =3.002-1.254, P < 0.01). The A value of SYN positive reactant at postoperative 2, 3 and 4 weeks was significantly higher than that at previous time point (t =2.011-2.716,P < 0.05-0.01). ② Test results of learning and memory ability: In the first test, there was no significant difference in the ratio of rats which kept memory ability between two groups (P > 0.05); In the third test, the ratio of rats which kept memory ability in the model group was significantly lower than that in the control group at postoperative 4 weeks and 2 months[53%(8/15),100%(15/15);60%(9/15),93%(14/15),χ 2=2.863,2.901,P < 0.01]. CONCLUSION: The destroyed hippocampal structure induces the decrease of learning and memory ability of developmental rats. Early interference can increase the quality of neurons and also promote functional development of the nervous system.

Development of Mathematical Model on Preparation of Functionally Graded Material by Co-sedimentation

From the process of sedimentation the mathematical relationships among deposition volume and powder properties as well as sedimentation parameters were deduced. Based on the formula a mathematical model was set up and simulated through the computer. At last the validity of mathematical model was supported by the representative experiment on Ti-Mo system FGM prepared by co-sedimentation.

Cloning GhSCFP Gene and Its Function in Cotton Fiber Development

As a major raw material for the textile industry and the most important fiber crop in the world,cotton is of great significance in Chinese economy.The development of cotton fiber can be divided

To Actively Perform the Judicial Administration Function and Promote the Development of Cause of Human Rights

Respect for human rights and protection of human rights are significant rules in the Constitution of the People’s Republic of China.In 2015,judicial administration departments at all levels legally exercised their duties,implemented the principles and rules in constitution,and kept strengthening propagation of human rights through creation of contents and methods which had acquired great effects.What they have done contributes significantly toward the development of human rights in China.

The type of lipid supplement has crucial implications for forage particle size in calf starter diets

Background Forage inclusion in starters of young dairy calves has become an acceptable strategy in the last decade.To compensate for the lower energy provided by forage,concurrent lipid supplementation can be proposed.How-ever,ruminal microbial activity and forage digestibility may be decreased by lipid supplementation.We hypothesized that the composite effect of forage and lipid supplements may be dependent on forage particle size and the type of lipid supplement.Therefore,we evaluated the effect of long(LP;geometric mean,4.97 mm)vs.short alfalfa hay particle sizes(SP;geometric mean,1.26 mm)with either soybean oil(SBO)or palm fatty acids(PLF)as lipid source in a 2×2 factorial design with treatments SP-SBO,SP-PLF,LP-SBO,and LP-PLF.Treatments(n=13 with 6 males and 7 females each)were offered to Holstein calves(3 days old)with equal amounts of lipid(25 g/kg DM)through-out the experimental period.The milk offering scheme(d 1 to 53)was equal for all groups.Data collection continued until 20 d post-weaning.Results Interaction between forage particle size and lipid supplement was significant for the following readouts:the highest and lowest starter intakes during the pre-weaning period occurred in LP-PLF and LP-SBO,respectively.This was associated with similarly contrasting changes in average daily gain(ADG)during the post-weaning period,body weight at the end of experiment,withers height,digestibility of organic matter and neutral detergent fiber,and blood serum concentrations of glucose,beta-hydroxybutyrate,and insulin during the pre-weaning period.Dur-ing both pre-and post-weaning periods,the highest and lowest urinary excretion of allantoin and total purine deriva-tives,representing microbial protein synthesis,were observed in LP-PLF and LP-SBO,respectively,indicating that those diets were most and least favorable for rumen development.Irrespective of forage particle size,supplemental SBO vs.PLF increased serum malondialdehyde as an oxidative stress indicator across periods,increased blood urea nitrogen and feed efficiency in the pre-weaning period,and reduced hip height during the post-weaning period.Conclusions It can be concluded that feeding a rumen-inert,mostly saturated fatty acid source with alfalfa hay as long particle size is recommended with view on performance,whereas a combination soybean oil rich in unsatu-rated fatty acids should not be provided to milk-fed Holstein calves together with long particle forage.Feeding soybean oil and alfalfa hay as long particles is not advisable mainly due to lower starter consumption and impaired development of ruminal function.If dietary supplementation of soybean oil is applied,incorporation of forage as small particles should be preferred to support rumen development.

Effects of a soybean milk product on feto-neonatal development in rats

Since estrogenic pollutants and phytoestrogens can cause the disorder of the reproductive system, the effects of a soybean milk product（Vegemil^（？） containing 162 ppm isoflavones） on the feto-neonatal development, including male reproductive function, were investigated. Pregnant rats were fed the soybean milk（5% or 100% in drinking water）from gestational day（GD） 6 to parturition or to post-natal day（PND） 56. Specifically, the rats were divided into 4 groups： the control group（drinking water）, the GD5% group（5% soybean milk during only the GD period）, the GDPND5% group（5% soybean milk during the GD and PND periods）, and the GD-PND100% group（100% soybean milk instead of water during the GD and PND periods）. During the gestational, lactational, and developmental periods, the reproductive and developmental parameters of dams and offspring were observed. Feeding soybean milk did not affect the birth and physical development of both male and female offspring. At PND57, the weights of the testes and epididymides of F1 males significantly increased by feeding a high concentration of the soybean milk（GDPND100%）. In addition, feeding of the soybean milk during both the GD and PND periods（GD-PND5% and GDPND100%） enhanced the sperm counts and motility. The results indicate that soybean milk is safe for embryos,fetuses, and offspring, and improves the post-generational development of male reproductive function.

Acetylglutamate kinase is required for both gametophyte function and embryo development in Arabidopsis thaliana

The specific functions of the genes encoding arginine biosynthesis enzymes in plants are not well characterized. We report the isolation and characterization of Arabidopsis thaliana N-acetylglutamate kinase （NAGK）, which catalyzes the second step of arginine biosynthesis. NAGK is a plastid-localized protein and is expressed during most developmental processes in Arabidopsis. Heterologous expression of the Arabidopsis NAGK gene in a NAGK-deficient Escherichia coli strain fully restores bacterial growth on arginine-deficient medium, nagk mutant pollen tubes grow more slowly than wild type pollen tubes and the phenotype is restored by either specifically through complementation by NAGK in pollen, or exogenous supplementation of arginine, nagk female gametophytes are defective in micropylar pollen tube guidance due to the fact that female gametophyte cell fate specification was specifically affected. Expression of NAGK in synergid cells rescues the defect of nagk female gametophytes. Loss- of-function of NAGK results in Arabidopsis embryos not developing beyond the four-celled embryo stage. The embryo-defective phenotype in nagk/NAGK plants cannot be rescued by watering nagk/NAGK plants with arginine or ornithine supplementation. In conclusion, our results reveal a novel role of NAGK and arginine in regulating gametophyte function and embryo development, and provide valuable insights into arginine transport during embryo development.

Cotton functional genomics reveals global insight into genome evolution and fiber development

Due to the economic value of natural textile fiber, cotton has attracted much research attention, which has led to the publication of two diploid genomes and two tetraploid genomes. These big data facilitate functional genomic study in cotton, and allow researchers to investigate cotton genome structure, gene expression, and protein function on the global scale using high-throughput methods. In this review, we summarized recent studies of cotton genomes. Population genomic analyses revealed the domestication history of cultivated upland cotton and the roles of transposable elements in cotton genome evolution.Alternative splicing of cotton transcriptomes was evaluated genome-widely. Several important gene families like MYC, NAC, Sus and GhPLDal were systematically identified and classified based on genetic structure and biological function. High-throughput proteomics also unraveled the key functional proteins correlated with fiber development. Functional genomic studies have provided unprecedented insights into global-scale methods for cotton research.

Scale,Site Selection and Function of Economic and Technological Development Zones

This article analyses different characteristics of scale,site selection and function of the current economic andtehnological development zones in our country.It further proposes that it is inappropriate for the economic andtechnological development zones to follow the“pattern of the special zones”,but a new pattern of“small scale,unitaryfunction and closeness to old city”should be adopted to fit in with the practical conditions in our country.The scale,landuse structure and the relations between the development zone and the old city are the basic issues in planning forthe development zones.These three essential elements constitute the pattern of planning which determines to a certainextent the success or failure of the development zones.

Plant MicroRNAs and Development

MicroRNAs（miRNAs） are a class of about 20—24 nt small non-coding RNAs that can regulate their target gene expression transcriptionally and posttranscriptionally.There are an increasing number of studies describing the identification of new components and regulatory mechanisms involved in the miRNA biogenesis and effector pathway as well as new functions of miRNAs in plant development. This review mainly focuses on the components involved in this pathway,and the developmental defects associated with the corresponding mutations.Some functions of important miRNAs in plant development,together with the modes of miRNA action,are also discussed in this review to describe the recent advance in this area.

1Chinese human geography and its contributions

The aim of this paper is to sift through examples of outstanding contributions made by Chinese human geography in terms of social applications and explain the basic concepts and theoretical methods explored by human geography that are behind the applications of results with major social influence, so as to be able to summarize the main school that represents developments in contemporary Chinese human geography. Chinese human geography upholds the subject＇s designation as being integrated and interdisciplinary. Research focuses on interactions between the natural and human spheres of the Earth＇s surface, and it is guided by the understanding and effects of the processes of regional sustainable development at different spatial scales. Chinese human geography has innovatively established the following development paradigm： ＂To be guided by application requirements, refine key issues of the discipline in the course of solving major issues of human geography in national and regional development, and by solving those key issues, to enhance its ability to provide scientific and technological support to serve national and local needs while promoting its own development.＂ Results from early Chinese human geography studies on land use and agricultural zoning, recent research results on point-axis system models and T-shaped national spatial development patterns, and current research results on territorial function theory and major function oriented zones have all continued and strengthened the mainstream school of Chinese human geography and have avoided the global tendency for human geography to become rapidly human-oriented, while results have been applied at the highest level of decision-making management. Chinese human geography can provide lessons for developing countries and may play a leading role in the future development of global human geography.