目的判断与R et、粘蛋白-1(M uc in-1)和半乳糖凝集素-3(G a lectin-3)蛋白识别的相关因素及影响甲状腺肿块复发的可能因素。方法观察R et、M uc in-1(MUC 1)和G a lectin-3(G a l-3)表达与甲状腺滤泡源性肿瘤相关指标的关系,并与随访...目的判断与R et、粘蛋白-1(M uc in-1)和半乳糖凝集素-3(G a lectin-3)蛋白识别的相关因素及影响甲状腺肿块复发的可能因素。方法观察R et、M uc in-1(MUC 1)和G a lectin-3(G a l-3)表达与甲状腺滤泡源性肿瘤相关指标的关系,并与随访资料相结合,分析甲状腺肿瘤有关的生物学行为。结果①运用多因素逐步log istic回归分析法,分别得出R et、MUC 1、G a l-3表达的log istic回归方程,结果包膜存在、最大直径是R et表达的抑制因素,包膜侵犯与双侧性对R et表达有促进作用。合并病变、包膜存在是MUC 1表达的抑制因素,包膜存在也是G a l-3表达的抑制因素,包膜侵犯对MUC 1和G a l-3表达有促进作用。②预后分析的Cox比例风险函数模型可知:有包膜比无包膜或包膜不完整者在某时刻复发的相对危险度为0.126,即复发的可能性降低了87.4%;病程每增加一个等级,在某时刻复发的相对危险度为2.701,即复发的可能性增加了1.701倍。结论①包膜存在与甲状腺肿瘤不或低表达R et、MUC 1和G a l-3关联;恶性肿瘤的包膜侵犯与恶性标志物表达密切关联;囊性变与MUC 1和R et的阴性表达有关;双侧性同一病变者R et表达的可能性增高;腺体外浸润、淋巴结转移者表达R et、MUC 1和G a l-3的机率增高,其关联性表明三种标志物能反映恶性肿瘤的生物学行为。②复发有关因素中有包膜者,不易复发;而及早诊治甲状腺病变,其术后复发可大大降低。展开更多
Objective:Because of the poor prognosis of lacrimal adenoid cystic carcinoma(LACC),we aimed to investigate the effects of perineural invasion(PNI)and consequent aberrations in GDNF/GFRα-1/RET protein expression on LA...Objective:Because of the poor prognosis of lacrimal adenoid cystic carcinoma(LACC),we aimed to investigate the effects of perineural invasion(PNI)and consequent aberrations in GDNF/GFRα-1/RET protein expression on LACC recurrence.Methods:Clinicopathological data for 51 histologically confirmed patients with LACC enrolled between 2001 and 2017 were retrospectively analyzed.Hematoxylin and eosin staining was applied to assess PNI.Tissue-based immunohistochemistry(IHC)detection of GDNF,GFRα-1,and RET proteins was performed on LACC formalin-fixed,paraffin-embedded specimens.We generated semi-quantitative data of the IHC results and compared them with the clinicopathological data for the 51 patients.Results:Of the 51 patients,19(37.3%)were PNI positive.Recurrence was more common for LACC with than without PNI(73.7%vs.37.5%,P=0.01).GDNF,GFRα-1,and RET proteins were expressed in 62.7%,62.7%,and 54.9%of the 51 patients with LACC,respectively.The expression of all 3 proteins was more common in patients with than without PNI.In agreement with previous findings,PNI-associated GFRα-1 and RET positivity,as detected by IHC,remained significantly associated with recurrence,whereas GDNF expression,as detected by IHC,was not correlated with LACC recurrence.Specifically,patients with concurrent GFRα-1 and RET expression may have a high risk of PNI(89.5%positivity rate)and recurrence(84.2%positivity rate).Conclusions:PNI may contribute to LACC recurrence.The concurrent expression of GFRα-1 and RET proteins,as detected by IHC,may potentially be associated with LACC PNI and recurrence.展开更多
文摘目的判断与R et、粘蛋白-1(M uc in-1)和半乳糖凝集素-3(G a lectin-3)蛋白识别的相关因素及影响甲状腺肿块复发的可能因素。方法观察R et、M uc in-1(MUC 1)和G a lectin-3(G a l-3)表达与甲状腺滤泡源性肿瘤相关指标的关系,并与随访资料相结合,分析甲状腺肿瘤有关的生物学行为。结果①运用多因素逐步log istic回归分析法,分别得出R et、MUC 1、G a l-3表达的log istic回归方程,结果包膜存在、最大直径是R et表达的抑制因素,包膜侵犯与双侧性对R et表达有促进作用。合并病变、包膜存在是MUC 1表达的抑制因素,包膜存在也是G a l-3表达的抑制因素,包膜侵犯对MUC 1和G a l-3表达有促进作用。②预后分析的Cox比例风险函数模型可知:有包膜比无包膜或包膜不完整者在某时刻复发的相对危险度为0.126,即复发的可能性降低了87.4%;病程每增加一个等级,在某时刻复发的相对危险度为2.701,即复发的可能性增加了1.701倍。结论①包膜存在与甲状腺肿瘤不或低表达R et、MUC 1和G a l-3关联;恶性肿瘤的包膜侵犯与恶性标志物表达密切关联;囊性变与MUC 1和R et的阴性表达有关;双侧性同一病变者R et表达的可能性增高;腺体外浸润、淋巴结转移者表达R et、MUC 1和G a l-3的机率增高,其关联性表明三种标志物能反映恶性肿瘤的生物学行为。②复发有关因素中有包膜者,不易复发;而及早诊治甲状腺病变,其术后复发可大大降低。
基金supported by the Tianjin Municipal Commission of Health and Family Planning Science Foundation Grant(Grant Nos.2015KZ100 and 2014KZ098)。
文摘Objective:Because of the poor prognosis of lacrimal adenoid cystic carcinoma(LACC),we aimed to investigate the effects of perineural invasion(PNI)and consequent aberrations in GDNF/GFRα-1/RET protein expression on LACC recurrence.Methods:Clinicopathological data for 51 histologically confirmed patients with LACC enrolled between 2001 and 2017 were retrospectively analyzed.Hematoxylin and eosin staining was applied to assess PNI.Tissue-based immunohistochemistry(IHC)detection of GDNF,GFRα-1,and RET proteins was performed on LACC formalin-fixed,paraffin-embedded specimens.We generated semi-quantitative data of the IHC results and compared them with the clinicopathological data for the 51 patients.Results:Of the 51 patients,19(37.3%)were PNI positive.Recurrence was more common for LACC with than without PNI(73.7%vs.37.5%,P=0.01).GDNF,GFRα-1,and RET proteins were expressed in 62.7%,62.7%,and 54.9%of the 51 patients with LACC,respectively.The expression of all 3 proteins was more common in patients with than without PNI.In agreement with previous findings,PNI-associated GFRα-1 and RET positivity,as detected by IHC,remained significantly associated with recurrence,whereas GDNF expression,as detected by IHC,was not correlated with LACC recurrence.Specifically,patients with concurrent GFRα-1 and RET expression may have a high risk of PNI(89.5%positivity rate)and recurrence(84.2%positivity rate).Conclusions:PNI may contribute to LACC recurrence.The concurrent expression of GFRα-1 and RET proteins,as detected by IHC,may potentially be associated with LACC PNI and recurrence.
