利用溶剂热法制备纳米Fe 3 O 4,并将其分散在正硅酸乙酯(TEOS)水解液中,在表面沉积一层SiO_(2).用甲基丙烯酰氧基丙基三甲氧基硅烷(MPS)对SiO_(2)-Fe 3 O 4表面改性,得到MPS-SiO_(2)-Fe 3 O 4.将甲基丙烯酸(MAA)与己烯雌酚(DES)以摩尔比...利用溶剂热法制备纳米Fe 3 O 4,并将其分散在正硅酸乙酯(TEOS)水解液中,在表面沉积一层SiO_(2).用甲基丙烯酰氧基丙基三甲氧基硅烷(MPS)对SiO_(2)-Fe 3 O 4表面改性,得到MPS-SiO_(2)-Fe 3 O 4.将甲基丙烯酸(MAA)与己烯雌酚(DES)以摩尔比4∶1加入乙腈溶剂中,进行12 h自组装,再加入MPS-SiO_(2)-Fe 3 O 4、乙二醇二甲基丙烯酸酯(EGDMA)和偶氮二异丁腈(AIBN),进行超声分散30 min,65℃条件下机械搅拌,反应24 h,制得DES磁性分子印迹聚合物(MMIP).采用透射电镜、振动样品磁强计和吸附试验等方法进行表征分析.结果表明:MMIP的饱和磁化强度为268 kA/m,无磁滞现象,矫顽力为0,表现出超顺磁性,在磁铁作用下17 s就可与溶液分离;室温下,MMIP对DES的静态最大吸附量为7.1 mg/g,动态吸附时MMIP在60 min时可以达到静态最大吸附量的90%以上;MMIP对DES的印迹因子为3.70,因而对DES有较大的识别能力;MMIP可重复使用6次以上,具有良好的再生循环性能.展开更多
为探究des(rhamnosyl)verbascoside体外抗乙型肝炎病毒(HBV)的活性作用和机制,本研究以des(rhamnosyl)verbascoside为实验药物对HepG2.2.15细胞进行干预,实验分为药物干预组和对照组,采用串联质谱标签(Tandem Mass Tag,TMT)蛋白质组学...为探究des(rhamnosyl)verbascoside体外抗乙型肝炎病毒(HBV)的活性作用和机制,本研究以des(rhamnosyl)verbascoside为实验药物对HepG2.2.15细胞进行干预,实验分为药物干预组和对照组,采用串联质谱标签(Tandem Mass Tag,TMT)蛋白质组学方法对提取的总蛋白进行分析。结果表明,共筛选得到300个差异表达蛋白,其中有109个上调蛋白,191个下调蛋白。基因本体论(Gene Ontology,GO)分析结果显示,差异蛋白主要参与DNA复制(DNA replication)、鞘糖脂代谢(Glycosphingolipid metabolic process)、细胞增殖(Cell proliferation)、寡糖分解代谢(Oligosaccharide catabolic process)等生物学过程,以及DNA聚合酶活性(DNA polymerase activity)、丝氨酸型羧肽酶活性(Serine type carboxypeptidase activity)、DNA引物酶活性(DNA primase activity)等分子功能。京都基因与基因组百科全书(Kyoto Encyclopedia of Genes and Genomes,KEGG)分析结果显示,差异蛋白主要参与细胞代谢(Metabolism)、遗传信息传导(Genetic information processing)、生物系统通路(Organismal systems)等相关信号通路。亚细胞定位分析表明,差异蛋白大多定位在细胞质和细胞核。本研究共筛选出13个与抗HBV密切相关的蛋白。通过定量蛋白组学初步揭示des(rhamnosyl)verbascoside可能通过增加HGF、SORT1、MAN2B1,减少PRIM1、PRIM2、POLA1、POLD3、POLD2、POLD1、POLE、ERCC2、LAMC1、SDC1等蛋白表达来起到体外抗HBV的作用。展开更多
This study conducts a comparative analysis between detached eddy simulation(DES)and Unsteady Reynolds-averaged Navier-Stokes(URANS)models for simulating pressure fluctuations in a stilling basin,aiming to assess the U...This study conducts a comparative analysis between detached eddy simulation(DES)and Unsteady Reynolds-averaged Navier-Stokes(URANS)models for simulating pressure fluctuations in a stilling basin,aiming to assess the URANS mode’s performance in modeling pressure fluctuation.The URANS model predicts accurately a smoother flow field and its time-average pressure,yet it underestimates the root mean square of pressure(RMSP)fluctuation,achieving approximately 70%of the results predicted by DES model on the bottom floor of the stilling basin.Compared with DES model’s results,which are in alignment with the Kolmogorov−5/3 law,the URANS model significantly overestimates low-frequency pulsations,particularly those below 0.1 Hz.We further propose a novel method for estimating the RMSP in the stilling basin using URANS model results,based on the establishment of a quantitative relationship between the RMSP,time-averaged pressure,and turbulent kinetic energy in the boundary layer.The proposed method closely aligns with DES results,showing a mere 15%error level.These findings offer vital insights for selecting appropriate turbulence models in hydraulic engineering and provide a valuable tool for engineers to estimate pressure fluctuation in stilling basins.展开更多
[Objectives]This study was conducted to explore rapid and large-scale screening and detection of peste des petits ruminants(PPR),so as to provide important technical means for prevention,control and purification of PP...[Objectives]This study was conducted to explore rapid and large-scale screening and detection of peste des petits ruminants(PPR),so as to provide important technical means for prevention,control and purification of PPR.[Methods]Soluble N protein and NH fusion protein were successfully obtained in an Escherichia coli expression system by optimizing E.coli codon and expression conditions.Furthermore,based on purified soluble N protein and NH fusion protein,a double-antigen sandwich time-resolved fluorescence immunoassay method for detection of peste des petits ruminants virus(PPRV)was established.[Results]The method has high sensitivity and specificity and can specifically detect the antibody against PPRV in sheep serum,and it has no cross reaction with other related diseases.The method was used to detect 292 clinical samples,and compared with French IDVET competition ELISA kit.The coincidence rates of positive samples and negative samples from the two kinds of test kits were 92.47%and 97.26%,respectively,and the overall coincidence rate was 94.86%.The intra-group and inter-group coefficients of variation in the repeatability test were less than 10%.[Conclusions]Compared with the traditional ELISA method,the double-antigen sandwich time-resolved fluorescence immunoassay for detection of PPRV has equivalent sensitivity and specificity,and simple and rapid operation,and thus high application and popularization value.展开更多
Background:Desmoid tumors are rare,locally aggressive,highly recurrent soft-tissue tumors without approved treatments.Methods:We conducted a phase 3,international,double-blind,randomized,placebo-controlled trial of ni...Background:Desmoid tumors are rare,locally aggressive,highly recurrent soft-tissue tumors without approved treatments.Methods:We conducted a phase 3,international,double-blind,randomized,placebo-controlled trial of nirogacestat in adults with progressing desmoid tumors according to the Response Evaluation Criteria in Solid Tumors,version 1.1.Patients were assigned in a 1:1 ratio to receive the oralγ-secretase inhibitor nirogacestat(150 mg)or placebo twice daily.The primary end point was progression-free survival.展开更多
Maternal exposure to estrogenic xenobiotics or phthalates has been implicated in the distortion of early male reproductive development, referred to in humans as the testicular dysgenesis syndrome. It is not known, how...Maternal exposure to estrogenic xenobiotics or phthalates has been implicated in the distortion of early male reproductive development, referred to in humans as the testicular dysgenesis syndrome. It is not known, however, whether such early gestational and/or lactational exposure can influence the later adult-type Leydig cell phenotype. In this study, Sprague-Dawley rats were exposed to dibutyl phthalate （DBP; from gestational day （GD） 14.5 to postnatal day （PND） 6） or diethylstilbestrol （DES; from GD14o5 to GD16.5） during a short gestationalllactational window, and male offspring subsequently analysed for various postnatal testicular parameters. All offspring remained in good health throughout the study. Maternal xenobiotic treatment appeared to modify specific Leydig cell gene expression in male offspring, particularly during the dynamic phase of mid-puberty, with serum INSL3 concentrations showing that these compounds led to a faster attainment of peak values, and a modest acceleration of the pubertal trajectory. Part of this effect appeared to be due to a treatment-specific impact on Leydig cell proliferation during puberty for both xenobiotics. Taken together, these results support the notion that maternal exposure to certain xenobiotics can also influence the development of the adult-type Leydig cell population, possibly through an effect on the Leydig stem cell population.展开更多
文摘利用溶剂热法制备纳米Fe 3 O 4,并将其分散在正硅酸乙酯(TEOS)水解液中,在表面沉积一层SiO_(2).用甲基丙烯酰氧基丙基三甲氧基硅烷(MPS)对SiO_(2)-Fe 3 O 4表面改性,得到MPS-SiO_(2)-Fe 3 O 4.将甲基丙烯酸(MAA)与己烯雌酚(DES)以摩尔比4∶1加入乙腈溶剂中,进行12 h自组装,再加入MPS-SiO_(2)-Fe 3 O 4、乙二醇二甲基丙烯酸酯(EGDMA)和偶氮二异丁腈(AIBN),进行超声分散30 min,65℃条件下机械搅拌,反应24 h,制得DES磁性分子印迹聚合物(MMIP).采用透射电镜、振动样品磁强计和吸附试验等方法进行表征分析.结果表明:MMIP的饱和磁化强度为268 kA/m,无磁滞现象,矫顽力为0,表现出超顺磁性,在磁铁作用下17 s就可与溶液分离;室温下,MMIP对DES的静态最大吸附量为7.1 mg/g,动态吸附时MMIP在60 min时可以达到静态最大吸附量的90%以上;MMIP对DES的印迹因子为3.70,因而对DES有较大的识别能力;MMIP可重复使用6次以上,具有良好的再生循环性能.
文摘为探究des(rhamnosyl)verbascoside体外抗乙型肝炎病毒(HBV)的活性作用和机制,本研究以des(rhamnosyl)verbascoside为实验药物对HepG2.2.15细胞进行干预,实验分为药物干预组和对照组,采用串联质谱标签(Tandem Mass Tag,TMT)蛋白质组学方法对提取的总蛋白进行分析。结果表明,共筛选得到300个差异表达蛋白,其中有109个上调蛋白,191个下调蛋白。基因本体论(Gene Ontology,GO)分析结果显示,差异蛋白主要参与DNA复制(DNA replication)、鞘糖脂代谢(Glycosphingolipid metabolic process)、细胞增殖(Cell proliferation)、寡糖分解代谢(Oligosaccharide catabolic process)等生物学过程,以及DNA聚合酶活性(DNA polymerase activity)、丝氨酸型羧肽酶活性(Serine type carboxypeptidase activity)、DNA引物酶活性(DNA primase activity)等分子功能。京都基因与基因组百科全书(Kyoto Encyclopedia of Genes and Genomes,KEGG)分析结果显示,差异蛋白主要参与细胞代谢(Metabolism)、遗传信息传导(Genetic information processing)、生物系统通路(Organismal systems)等相关信号通路。亚细胞定位分析表明,差异蛋白大多定位在细胞质和细胞核。本研究共筛选出13个与抗HBV密切相关的蛋白。通过定量蛋白组学初步揭示des(rhamnosyl)verbascoside可能通过增加HGF、SORT1、MAN2B1,减少PRIM1、PRIM2、POLA1、POLD3、POLD2、POLD1、POLE、ERCC2、LAMC1、SDC1等蛋白表达来起到体外抗HBV的作用。
基金Project supported by the Key Research and Development Plan Project of China(Grant No.2022YFC3204602)the National Natural Science Foundation of China(Grant No.U21A20157).
文摘This study conducts a comparative analysis between detached eddy simulation(DES)and Unsteady Reynolds-averaged Navier-Stokes(URANS)models for simulating pressure fluctuations in a stilling basin,aiming to assess the URANS mode’s performance in modeling pressure fluctuation.The URANS model predicts accurately a smoother flow field and its time-average pressure,yet it underestimates the root mean square of pressure(RMSP)fluctuation,achieving approximately 70%of the results predicted by DES model on the bottom floor of the stilling basin.Compared with DES model’s results,which are in alignment with the Kolmogorov−5/3 law,the URANS model significantly overestimates low-frequency pulsations,particularly those below 0.1 Hz.We further propose a novel method for estimating the RMSP in the stilling basin using URANS model results,based on the establishment of a quantitative relationship between the RMSP,time-averaged pressure,and turbulent kinetic energy in the boundary layer.The proposed method closely aligns with DES results,showing a mere 15%error level.These findings offer vital insights for selecting appropriate turbulence models in hydraulic engineering and provide a valuable tool for engineers to estimate pressure fluctuation in stilling basins.
基金Supported by National Key R&D Program for the Prevention and Control of Major Exotic Animal Diseases(2022YFD1800500)National Mutton Sheep Industrial Technology System(CARS39)+2 种基金Key Research and Development Program of Shandong Province(Major Science and Technology Innovation Project)(2021CXGC011306)Scientific Research Project of General Administration of Customs(2024HK033)Scientific Research Project of Jinan Customs(2023JK005).
文摘[Objectives]This study was conducted to explore rapid and large-scale screening and detection of peste des petits ruminants(PPR),so as to provide important technical means for prevention,control and purification of PPR.[Methods]Soluble N protein and NH fusion protein were successfully obtained in an Escherichia coli expression system by optimizing E.coli codon and expression conditions.Furthermore,based on purified soluble N protein and NH fusion protein,a double-antigen sandwich time-resolved fluorescence immunoassay method for detection of peste des petits ruminants virus(PPRV)was established.[Results]The method has high sensitivity and specificity and can specifically detect the antibody against PPRV in sheep serum,and it has no cross reaction with other related diseases.The method was used to detect 292 clinical samples,and compared with French IDVET competition ELISA kit.The coincidence rates of positive samples and negative samples from the two kinds of test kits were 92.47%and 97.26%,respectively,and the overall coincidence rate was 94.86%.The intra-group and inter-group coefficients of variation in the repeatability test were less than 10%.[Conclusions]Compared with the traditional ELISA method,the double-antigen sandwich time-resolved fluorescence immunoassay for detection of PPRV has equivalent sensitivity and specificity,and simple and rapid operation,and thus high application and popularization value.
文摘Background:Desmoid tumors are rare,locally aggressive,highly recurrent soft-tissue tumors without approved treatments.Methods:We conducted a phase 3,international,double-blind,randomized,placebo-controlled trial of nirogacestat in adults with progressing desmoid tumors according to the Response Evaluation Criteria in Solid Tumors,version 1.1.Patients were assigned in a 1:1 ratio to receive the oralγ-secretase inhibitor nirogacestat(150 mg)or placebo twice daily.The primary end point was progression-free survival.
文摘Maternal exposure to estrogenic xenobiotics or phthalates has been implicated in the distortion of early male reproductive development, referred to in humans as the testicular dysgenesis syndrome. It is not known, however, whether such early gestational and/or lactational exposure can influence the later adult-type Leydig cell phenotype. In this study, Sprague-Dawley rats were exposed to dibutyl phthalate （DBP; from gestational day （GD） 14.5 to postnatal day （PND） 6） or diethylstilbestrol （DES; from GD14o5 to GD16.5） during a short gestationalllactational window, and male offspring subsequently analysed for various postnatal testicular parameters. All offspring remained in good health throughout the study. Maternal xenobiotic treatment appeared to modify specific Leydig cell gene expression in male offspring, particularly during the dynamic phase of mid-puberty, with serum INSL3 concentrations showing that these compounds led to a faster attainment of peak values, and a modest acceleration of the pubertal trajectory. Part of this effect appeared to be due to a treatment-specific impact on Leydig cell proliferation during puberty for both xenobiotics. Taken together, these results support the notion that maternal exposure to certain xenobiotics can also influence the development of the adult-type Leydig cell population, possibly through an effect on the Leydig stem cell population.
