Biological Significance of [^(14)C]Phenol Accumulation in Different Organs of a Murrel, Channa punctatus, and the Common Carp, Cyprinus carpio

Phenol, a ubiquitous component of industrial effluents, is a common pollutant of water resources and a serious threat to fish.The present work demonstrates that a significant amount of phenol is retained by various tissues of the common carp.Cyprinus carpio.and the snake-headed murrel.Channa punclatus.The rate of [^(14)C] phenol accumulation was higher in the carp than in the murrel.It is suggested that retention of phenol in the brain and ovary may seriously afiect the reproductive potential of the fish. 1990 Academic Press.Inc.

IN VIVO COMPARATIVE OBSERVATION ON THE INVASIVENESS OF VARIOUS ORGANS BY DIFFERENT LEUKEMIA CELLS

Using patho-morphological method and transplantation bio-assay, the in vivo invasiveness of leukemia cells is three transplantable mouse T cell leukemia models was comparatively studied. The results showed that the invasion to the liver was consistent, but that to other organs was obviously different. L615 and L7212 leukemia cells preferred to the bone marrow and spleen than to the peritoneum while L7811 leukemia cells were just the opposite. Transplantation bio-assay demonstrated that leukemia cells were present in the bone marrow of L615 mice as early as 6 hours after leukemic cell inoculation, but no leukemia cells was detected in bone marrow of L7811 mice 2 days after inoculation. In the terminal phase, L615 mice bone marrow became filled with leukemia cells, but L7811 mice bone marrow contained only a few leukemia cells. The difference of invasiveness of leukemia cells among organs is probably related to "homing" receptor. The same type of leukemia cells may possess multiple "homing" receptor.

Identification and Transcriptional Regulation of CAMTA Genes in Liriodendron chinense

This study explores CAMTA genes in the rare and endangered Chinese plant species,Liriodendron chinense.Despite the completion of whole-genome sequencing,the roles of CAMTA genes in calcium regulation and stress responses in this species remain largely unexplored.Within the L.chinense genome,we identified two CAMTA genes,Lchi09764 and Lchi222536,characterized by four functional domains:CG-1,TIG,ANK repeats,and IQ motifs.Our analyses,including phylogenetic investigations,cis-regulatory element analyses,and chromosomal location studies,aim to elucidate the defining features of CAMTA genes in L.chinense.Applying Weighted Gene Co-Expression Network Analysis(WGCNA),we explored the impact of CAMTA genes on different organs and their regulation under abiotic stress conditions.The identification of significant gene modules and the prediction of promoter binding sites revealed co-expressed genes associated with CAMTA transcription factors.In summary,this study provides initial insights into CAMTA genes in L.chinense,laying the groundwork for future research on their evolution and biological roles.This knowledge enhancement contributes to a better understanding of plant responses to environmental stress—an essential aspect of plant biology.

Compositional and Structural Difference of Fulvic Acid from Black Soil Applied with Different Organic Materials: Assessment After Three Years

Knowledge of different effects of various organic materials on soil humic substance is important for both environmental safety and sustainable agriculture.A pot experiment was conducted at Jilin Agricultural University,Jilin Province in northeast China to discover the influence of herb residue,animal excrement,woody residue,animal remnant on fulvic acid（FA） composition and structure using differential thermal analysis-thermogravimetric（DTA-TG）,fourier transform infrared spectroscopy（FTIR） and elemental analysis.DTA-TG showed the range of peak temperature in the first exothermic reaction increased following the trend： CK〉herb residue〉animal excrement〉woody residue=animal remnant,and the most weight loss was observed in animal excrement.Moreover,the second exothermic reaction of CK-and animal excrement-FA was presented as double peaks,the order of weight loss in that area was animal remnant〉CK〉woody residue〉animal excrement〉herb residue.According to FTIR,herb residue displayed higher adsorption intensity at 2 950,1 420,1 240 and 1 030 cm-1,animal excrement was in reverse.At the same time,herb residue-and animal excrement-FA had an absorption peak at 1 720 cm-1,while other organic materials didn＇t have this peak.As elemental analysis showed,FA isolated from various treatments was significantly distinct.It was clearly shown from our results that FA composition and structure in amended soils may be affected in different ways and at various extents on dependence of the nature and origin of amendment.

Mutagenicity and Genotoxicity of Organic Extracts from Finished Water with Different Treatment Process

Purification of surface water is widely practiced with conventional water treatment processes like coagulation-flocculation, sedimentation, filtration,and disinfection. Some reports have specified that conventional wastewater purification processes do not effectively remove many chemical contaminants,

Clinical analysis of different periods of liver transplantation at an organ transplantation centre

Objective To summarize our clinical experience in liver transplantation while considering the background in this filed in China. Methods Ninety-five patients who had received liver transplantation from April 1993 to March 2002 were analyzed retrospectively. Three periods were defined objectively as period Ⅰ(1993-1997),Ⅱ(1999) and Ⅲ(2000 -2002). Operative techniques, recipients, original diseases, complications and survival rates were compared among the three periods. Results Malignant liver lesions were the main cause for liver transplantation in period Ⅰ and Ⅱ. The ratio of number of malignant disease to total recipients decreased gradually from period Ⅰ to Ⅱ (100%, 53% and 35%, respectively). The 1-year survival rate in patients with benign liver disease was 85 % and the total operative mortality was 5% in period Ⅲ. The incidence of hepatitis B virus reactivation or reinfection was 24% twelve months after liver transplantation. Vascular complication decreased but biliary complications did

Effect of Dissolved Organic Matter on Titanium Dioxide Nanoparticles in Aquatic Environment:Molecular Weight Fractions

At present,a growing number of consumer products contain engineered nanoparticle TiO2(nano⁃TiO2),which has resulted in the consequences of nano⁃TiO2 entering the aquatic environment directly or indirectly at some stage.The fate of nano⁃TiO2 in the aquatic environment has become the key factor which affects its safety application and nanoecotoxicology.This paper aims to investigate how the dissolved organic matters(DOM),especially the molecular weight fractions in the aquatic environment,affect the aggregation,stability,and fate of nano⁃TiO2,and the interaction mechanism of DOM and nano⁃TiO2.Results of dynamic light scattering(DLS)showed that the molecular weight of DOM molecules caused different aggregation rates of nano⁃TiO2 in aqueous solution.Fourier Transform infrared spectroscopy(FTIR)results indicated the molecular structure is characteristics of DOM fractions and the mechanisms of bonds formation between DOM and nano⁃TiO2.Results of three⁃dimensional excitation⁃emission matrices(3D⁃EEM)confirmed the FTIR results and implied the increase of the stability of theπ-πconjugated system in the presence of DOM.In addition,low molecular weight of DOM fractions appeared to show more affinity with nano⁃TiO2 than high molecular weight fractions.

Significance of adverse outcome pathways in biomarker-based environmental risk assessment in aquatic organisms

In environmental risk assessments（ERA）, biomarkers have been widely used as an early warning signal of environmental contamination. However, biomarker responses have limitation due to its low relevance to adverse outcomes（e.g., fluctuations in community structure, decreases in population size, and other similar ecobiologically relevant indicators of community structure and function）. To mitigate these limitations, the concept of adverse outcome pathways（AOPs） was developed. An AOP is an analytical, sequentially progressive pathway that links a molecular initiating event（MIE） to an adverse outcome. Recently, AOPs have been recognized as a potential informational tool by which the implications of molecular biomarkers in ERA can be better understood. To demonstrate the utility of AOPs in biomarker-based ERA, here we discuss a series of three different biological repercussions caused by exposure to benzo（a）pyrene（Ba P）, silver nanoparticles（Ag NPs）, and selenium（Se）. Using mainly aquatic invertebrates and selected vertebrates as model species, we focus on the development of the AOP concept. Aquatic organisms are suitable bioindicator species whose entire lifespans can be observed over a short period; moreover, these species can be studied on the molecular and population levels.Also, interspecific differences between aquatic organisms are important to consider in an AOP framework, since these differences are an integral part of the natural environment.The development of an environmental pollutant-mediated AOP may enable a better understanding of the effects of environmental pollutants in different scenarios in the diverse community of an ecosystem.

Detecting the storage and change on topsoil organic carbon in grasslands of Inner Mongolia from 1980s to 2010s

Soil carbon sequestration and potential has been a focal issue in global carbon research. Under the background of global change, the estimation of the size as well as its change of soil organic carbon（SOC） storage is of great importance. Based on soil data from the second national soil survey and field survey during 2011–2012, by using the regression method between sampling soil data and remote sensing data, this paper aimed to investigate spatial distribution and changes of topsoil（0–20 cm） organic carbon storage in grasslands of Inner Mongolia between the 1980 s and 2010 s. The results showed that：（1） the SOC storage in grasslands of Inner Mongolia between the 1980 s and 2010 s was estimated to be 2.05 and 2.17 Pg C, with an average density of 3.48 and 3.69 kg C·m–2, respectively. The SOC storage was mainly distributed in the typical steppe and meadow steppe, which accounted for over 98% of the total SOC storage. The spatial distribution showed a decreased trend from the meadow steppe, typical steppe to the desert steppe, corresponding to the temperature and precipitation gradient.（2） SOC changes during 1982–2012 were estimated to be 0.12 Pg C, at 7.00 g C·m–2·yr–1, which didn＇t show a significant change, indicating that SOC storage in grasslands of Inner Mongolia remained relatively stable over this period. However, topsoil organic carbon showed different trends of carbon source/sink during the past three decades. Meadow steppe and typical steppe had sequestered 0.15 and 0.03 Pg C, respectively, served as a carbon sink; while desert steppe lost 0.06 Pg C, served as a carbon source. It appears that SOC storage in grassland ecosystem may respond differently to climate change, related to vegetation type, regional climate type and grazing intensity. These results might give advice to decision makers on adopting suitable countermeasures for sustainable grassland utilization and protection.