The rapidly developing rural tourism industry in China has deeply influenced the livelihoods of rural households. This study compares the impact of rural tourism development in poor areas on the farmers' livelihoo...The rapidly developing rural tourism industry in China has deeply influenced the livelihoods of rural households. This study compares the impact of rural tourism development in poor areas on the farmers' livelihoods, based on the Sustainable Livelihoods Framework proposed by the United Kingdom's Department for International Development. Gougezhuang, a tourist village, and Jiaojiekou, a non-tourist village, both located in Yesanpo Tourism District in the state-level povertystricken Laishui County, Hebei Province, were selected as case studies. The livelihood models of the two villages show great differentiation after 20 years of tourism development. Gougezhuang Village has been driven by the tourism industry and farmers are employed locally, enjoying a relatively higher-income life, with a developmental livelihood model. In contrast, farmers in Jiaojiekou Village earn their living mainly by leaving home to work in the city. They have a low income, with a survival livelihood model, supported by part-time business. Considering that the two villages are adjacent and have similar development backgrounds, the analysis results indicate that rural tourism has an apparent locationspecific effect on poverty alleviation and socioeconomic development, but the development ofrural tourism is limited regarding large-scale poverty alleviation efforts in poor areas. To implement a propoor-tourism strategy and achieve sustainable development, it is necessary to implement comprehensive management measures to change the local conditions of rural settlements and make full use of the spillover effect of tourist consumption.展开更多
We utilized a unique culture system to analyze the expression patterns of gene, protein, and cell surface antigen, and the biological process of the related genes in erythroid and myeloid differentiation and switching...We utilized a unique culture system to analyze the expression patterns of gene, protein, and cell surface antigen, and the biological process of the related genes in erythroid and myeloid differentiation and switching of hematopoietic stem cells （HSCs） in response to cytokine alterations. Gene-specific fragments （266） identified from five populations of cytokine-stimulated HSCs were categorized into three groups： （1） expressed specifically in a single cell population; （2） expressed in two cell populations, and （3） expressed in three or more populations. Of 145 defined cDNAs, three （2%） were novel genes. Protein two-dimensional gel electrophoresis and flow cytometry analyses showed overlapped and distinguished protein expression profiles in the cell populations studied. Biological process mapping of mRNAs expressed in erythroid and myeloid lineages indicated that mRNAs shared by both lineages attended ＇core processes,＇ whereas genes specifically expressed in either lineage alone were related to specific processes or cellular maturation. Data from this study support the hypothesis that committed HSCs （El4 or G14） cells can still be redirected to develop into myeloid or erythroid cells when erythropoietin （EPO） is replaced with granulocyte-colony stimulating factor （G-CSF） under erythroid-cultured condition or G-CSF with EPO in myeloid-cultured environment, respectively. Our results suggest that genes or proteins co-expressed in erythroid and myeloid lineages may be essential for the lineage maintenance and switching in hematopoiesis.展开更多
The Chinese crested duck is a unique duck breed having a bulbous feather shape on its duck head.However,the mechanisms involved in its formation and development are unclear.In the present study,RNA sequencing analysis...The Chinese crested duck is a unique duck breed having a bulbous feather shape on its duck head.However,the mechanisms involved in its formation and development are unclear.In the present study,RNA sequencing analysis was performed on the crested tissues of 6 Chinese crested ducks and the scalp tissues of 6 cherry valley ducks(CVs)from 2 developmental stages.This study identified 261 differentially expressed genes(DEGs),122 upregulated and 139 downregulated,in the E28 stage and 361 DEGs,154 upregulated and 207 downregulated in the D42 stage between CC and CV ducks.The subsequent results of weighted gene co-expression network analysis(WGCNA)revealed that the turquoise and cyan modules were associated with the crest trait in the D42 stage,meanwhile,the green,brown,and pink modules were associated with the crest trait in the E28 stage.Venn analysis of the DEGs and WGCNA showed that 145 and 45 genes are associated between the D42 and E28 stages,respectively.The expression of WNT16,BMP2,SLC35F2,SLC6A15,APOBEC2,ABHD6,TNNC2,MYL1,and TNNI2 were verified by real-time quantitative PCR.This study provides an approach to reveal the molecular mechanisms underlying the crested trait development.展开更多
基金supported by the National Natural Science Foundation of China (Grant no. 41671151)the National Social Science Foundation of China (Grant no. 17BGL123)+1 种基金the Key Project of China National Tourism Administration Research Foundation (Grant no. 16TAAK004)the Tourism Young Expert Training Program of China National Tourism Administration (TYETP201527)
文摘The rapidly developing rural tourism industry in China has deeply influenced the livelihoods of rural households. This study compares the impact of rural tourism development in poor areas on the farmers' livelihoods, based on the Sustainable Livelihoods Framework proposed by the United Kingdom's Department for International Development. Gougezhuang, a tourist village, and Jiaojiekou, a non-tourist village, both located in Yesanpo Tourism District in the state-level povertystricken Laishui County, Hebei Province, were selected as case studies. The livelihood models of the two villages show great differentiation after 20 years of tourism development. Gougezhuang Village has been driven by the tourism industry and farmers are employed locally, enjoying a relatively higher-income life, with a developmental livelihood model. In contrast, farmers in Jiaojiekou Village earn their living mainly by leaving home to work in the city. They have a low income, with a survival livelihood model, supported by part-time business. Considering that the two villages are adjacent and have similar development backgrounds, the analysis results indicate that rural tourism has an apparent locationspecific effect on poverty alleviation and socioeconomic development, but the development ofrural tourism is limited regarding large-scale poverty alleviation efforts in poor areas. To implement a propoor-tourism strategy and achieve sustainable development, it is necessary to implement comprehensive management measures to change the local conditions of rural settlements and make full use of the spillover effect of tourist consumption.
文摘We utilized a unique culture system to analyze the expression patterns of gene, protein, and cell surface antigen, and the biological process of the related genes in erythroid and myeloid differentiation and switching of hematopoietic stem cells （HSCs） in response to cytokine alterations. Gene-specific fragments （266） identified from five populations of cytokine-stimulated HSCs were categorized into three groups： （1） expressed specifically in a single cell population; （2） expressed in two cell populations, and （3） expressed in three or more populations. Of 145 defined cDNAs, three （2%） were novel genes. Protein two-dimensional gel electrophoresis and flow cytometry analyses showed overlapped and distinguished protein expression profiles in the cell populations studied. Biological process mapping of mRNAs expressed in erythroid and myeloid lineages indicated that mRNAs shared by both lineages attended ＇core processes,＇ whereas genes specifically expressed in either lineage alone were related to specific processes or cellular maturation. Data from this study support the hypothesis that committed HSCs （El4 or G14） cells can still be redirected to develop into myeloid or erythroid cells when erythropoietin （EPO） is replaced with granulocyte-colony stimulating factor （G-CSF） under erythroid-cultured condition or G-CSF with EPO in myeloid-cultured environment, respectively. Our results suggest that genes or proteins co-expressed in erythroid and myeloid lineages may be essential for the lineage maintenance and switching in hematopoiesis.
基金supported by the earmarked fund for CARS,China(CARS-42)the earmarked fund for Jiangsu Agricultural Industry Technology System,China(JATS(2022)331)the Jiangsu Key Research and Development Program,China(BE2021332)。
文摘The Chinese crested duck is a unique duck breed having a bulbous feather shape on its duck head.However,the mechanisms involved in its formation and development are unclear.In the present study,RNA sequencing analysis was performed on the crested tissues of 6 Chinese crested ducks and the scalp tissues of 6 cherry valley ducks(CVs)from 2 developmental stages.This study identified 261 differentially expressed genes(DEGs),122 upregulated and 139 downregulated,in the E28 stage and 361 DEGs,154 upregulated and 207 downregulated in the D42 stage between CC and CV ducks.The subsequent results of weighted gene co-expression network analysis(WGCNA)revealed that the turquoise and cyan modules were associated with the crest trait in the D42 stage,meanwhile,the green,brown,and pink modules were associated with the crest trait in the E28 stage.Venn analysis of the DEGs and WGCNA showed that 145 and 45 genes are associated between the D42 and E28 stages,respectively.The expression of WNT16,BMP2,SLC35F2,SLC6A15,APOBEC2,ABHD6,TNNC2,MYL1,and TNNI2 were verified by real-time quantitative PCR.This study provides an approach to reveal the molecular mechanisms underlying the crested trait development.
