Objective To investigate th e value of proton magnetic resonance spectroscopy ( 1H-MRS) on diagnosis a nd differential diagnosis of the intracranial diseases by the MRS results of 52 patients. Methods 12 patients ...Objective To investigate th e value of proton magnetic resonance spectroscopy ( 1H-MRS) on diagnosis a nd differential diagnosis of the intracranial diseases by the MRS results of 52 patients. Methods 12 patients with benign glioma, 16 patients with malignant glioma, 10 patients with meningioma, 8 patients with virus encephalitis, and 6 patients with cerebral infarction underwent MRS in th e lesion region. We measured the area within the spectra of N-acetyl-aspartate (NAA), creatine/phosphocreatine (Cr), choline compounds (Cho), and lactate (Lac ). Results The spectra of meningiomas were characterized by abs ence of NAA. The spectra of gliomas were characterized by the decrease of NAA an d Cr, but the increase of Cho. The ratio of Cho to Cr was 2.25±1.21 in benign g liomas, while the ratio of Cho to Cr was 4.65±2.21 in malignant gliomas. The sp ectra of virus encephalitis appeared the decrease of NAA and the normality of Cr , with the 1.25±0.21 ratio of Cho/Cr. The apparent Lac wave could be seen in al l cerebral infarctions. Conclusion The value of 1H-MRS plays a significant role in the diagnosis and differential diagnosis of gliomas, mening iomas, virus encephalitis, and cerebral infarctions.展开更多
The argyrophilic staining technique was used in differential diagnosis between non-odgkin's lymphoraa (NHL) and reactive hyperplasia (RH) of lymphatic tissues in 30 cases of RH and 71 cases of NHL which were class...The argyrophilic staining technique was used in differential diagnosis between non-odgkin's lymphoraa (NHL) and reactive hyperplasia (RH) of lymphatic tissues in 30 cases of RH and 71 cases of NHL which were classified as low (11 cases), moderate (49 cases) and high malignant (11 cases) groups. The mumber and the area of Ag nucleolar organizer region (AgNORs) in 100 cells of each case were examined by both IBAS-200 cytoimage analyser and microscope. The results showed that there was significant difference in the number, but not in area, of AgNOR granules between RH and low malignant group of NHL (P< 0.001). In NHL, the significant differences in the number and area of AgNORs were found between each groups (P<0.05 or P< 0.001). It was also observed that in NHL, the number of AgNOR was negatively correlated with the area of the granules, and the shapes and distribution patterns of AgNOR were significantly different between each groups. Our results suggest that the quantitative study of AgNOR combining with the histological characteristics is very helpful in the differential diagnosis of RH and NHL and in the classification of NHL.展开更多
It is difficult to make a precise diagnosis of intestinal tuberculosis and to differentiate it from Crohn's disease. For evaluating Polymerase Chain Reaction (PCR) assay in these two aspects, 36 specimens of intes...It is difficult to make a precise diagnosis of intestinal tuberculosis and to differentiate it from Crohn's disease. For evaluating Polymerase Chain Reaction (PCR) assay in these two aspects, 36 specimens of intestinal tuberculosis from surgical resections and endoscopic biopsies and 26 Crohn's disease samples were subjected to PCR assay. 21 specimens of normal colon tissue surrounding cancer were used as the control. Oligonucleotides derived from the IS 6110 sequence, which is repeated in M. tuberculosis chromosome and highly specific for the M. tuberculosis complex, were used as a primer. The amplified PCR products were detected by examination of ethidium-bromide-stained polyacrylamide gels. The specificity of PCR products was confirmed by digestion with Sal 1 restrictive endonuclease and southern blot hybridization using digoxigenin-labeled probe. The results showed that the M. tuberculosis DNA was identified in 27 / 36 intestinal tuberculosis, but none of 26 Crohn's disease. Acid fast bacilli were only found in 16 / 36 intestinal tuberculosis. In conclusion, as a rapid, sensitive, and specific pathogenic method in diagnosis of intestinal tuberculosis, PCR assay has been developed in this study, and is considered valuable in the differentiation between intestinal tuberculosis and Crohn's disease.展开更多
文摘Objective To investigate th e value of proton magnetic resonance spectroscopy ( 1H-MRS) on diagnosis a nd differential diagnosis of the intracranial diseases by the MRS results of 52 patients. Methods 12 patients with benign glioma, 16 patients with malignant glioma, 10 patients with meningioma, 8 patients with virus encephalitis, and 6 patients with cerebral infarction underwent MRS in th e lesion region. We measured the area within the spectra of N-acetyl-aspartate (NAA), creatine/phosphocreatine (Cr), choline compounds (Cho), and lactate (Lac ). Results The spectra of meningiomas were characterized by abs ence of NAA. The spectra of gliomas were characterized by the decrease of NAA an d Cr, but the increase of Cho. The ratio of Cho to Cr was 2.25±1.21 in benign g liomas, while the ratio of Cho to Cr was 4.65±2.21 in malignant gliomas. The sp ectra of virus encephalitis appeared the decrease of NAA and the normality of Cr , with the 1.25±0.21 ratio of Cho/Cr. The apparent Lac wave could be seen in al l cerebral infarctions. Conclusion The value of 1H-MRS plays a significant role in the diagnosis and differential diagnosis of gliomas, mening iomas, virus encephalitis, and cerebral infarctions.
文摘The argyrophilic staining technique was used in differential diagnosis between non-odgkin's lymphoraa (NHL) and reactive hyperplasia (RH) of lymphatic tissues in 30 cases of RH and 71 cases of NHL which were classified as low (11 cases), moderate (49 cases) and high malignant (11 cases) groups. The mumber and the area of Ag nucleolar organizer region (AgNORs) in 100 cells of each case were examined by both IBAS-200 cytoimage analyser and microscope. The results showed that there was significant difference in the number, but not in area, of AgNOR granules between RH and low malignant group of NHL (P< 0.001). In NHL, the significant differences in the number and area of AgNORs were found between each groups (P<0.05 or P< 0.001). It was also observed that in NHL, the number of AgNOR was negatively correlated with the area of the granules, and the shapes and distribution patterns of AgNOR were significantly different between each groups. Our results suggest that the quantitative study of AgNOR combining with the histological characteristics is very helpful in the differential diagnosis of RH and NHL and in the classification of NHL.
文摘It is difficult to make a precise diagnosis of intestinal tuberculosis and to differentiate it from Crohn's disease. For evaluating Polymerase Chain Reaction (PCR) assay in these two aspects, 36 specimens of intestinal tuberculosis from surgical resections and endoscopic biopsies and 26 Crohn's disease samples were subjected to PCR assay. 21 specimens of normal colon tissue surrounding cancer were used as the control. Oligonucleotides derived from the IS 6110 sequence, which is repeated in M. tuberculosis chromosome and highly specific for the M. tuberculosis complex, were used as a primer. The amplified PCR products were detected by examination of ethidium-bromide-stained polyacrylamide gels. The specificity of PCR products was confirmed by digestion with Sal 1 restrictive endonuclease and southern blot hybridization using digoxigenin-labeled probe. The results showed that the M. tuberculosis DNA was identified in 27 / 36 intestinal tuberculosis, but none of 26 Crohn's disease. Acid fast bacilli were only found in 16 / 36 intestinal tuberculosis. In conclusion, as a rapid, sensitive, and specific pathogenic method in diagnosis of intestinal tuberculosis, PCR assay has been developed in this study, and is considered valuable in the differentiation between intestinal tuberculosis and Crohn's disease.
