[ Objective] This study aimed to investigate the effects of EMS (ethyl methane sulfonate) treatments on multiplication and differentiation of sugarcane embryonic cell clusters. [ Method] Sugarcane variety Xintaitang...[ Objective] This study aimed to investigate the effects of EMS (ethyl methane sulfonate) treatments on multiplication and differentiation of sugarcane embryonic cell clusters. [ Method] Sugarcane variety Xintaitang 22 (ROC22) was used as the experimental material. After treated with different concentrations of EMS for different time, sugarcane embryonic cell clusters were collected for subculture, differentiation and rooting, to compare and analyze the correlation of differ- ent EMS treatments with the muhiplicafion and differentiation of sugarcane embryonic cell clusters. [ Result] Treating ROC22 embryonic cell clusters with 0. 10% -0.15% EMS for4-6 h led to the best results, which reached the level of semi-lethal dose. Sugarcane embryonic cell clusters treated with EMS were adopted for subculture; results indicated that browning rate of cell clusters was higher than that in control (CK) but embryonic structure proportion was lower than that in control ; in addition, multiplication multiple of sugarcane embryonic cell clusters was also lower than that in control. After treated with EMS, sugarcane em- bryonic cell clusters exhibited significantly lower bud differentiation rate and higher browning rate compared with control. Furthermore, treating sugarcane embryonic cell clusters with 0.15% EMS for 2 h was conducive to plantlet emergence and rooting of sugarcane embryonic cell clusters. [ Conclusion] This study provided the- oretical basis for effective mutagenesis of sugarcane using EMS.展开更多
In this paper, we study the existence of multiple positive periodic solutions for the second order differential equation x′′(t) + p(t)x′(t) + q(t)x(t) = f(t, x(t)).By using Krasnoselskii fixed point...In this paper, we study the existence of multiple positive periodic solutions for the second order differential equation x′′(t) + p(t)x′(t) + q(t)x(t) = f(t, x(t)).By using Krasnoselskii fixed point theorem, we establish some criteria for the existence and multiple positive periodic solutions for this differential equation.展开更多
Recently, a multiple symbol differential (MSD) sphere decoding (SD) algorithm for unitary spacetime modulation over quasi-static channel has been proved to achieve the performance of maximumlikelihood (ML) detec...Recently, a multiple symbol differential (MSD) sphere decoding (SD) algorithm for unitary spacetime modulation over quasi-static channel has been proved to achieve the performance of maximumlikelihood (ML) detection with relatively low complexity. However, an error floor occurs if the algorithm is applied over rapid-fading channels. Based on the assumption of continuous fading, a multiple symbol differential automatic sphere decoding (MSDASD) algorithm is developed by incorporating a recursive form of an ML metric into automatic SD (ASD) algorithm. Furthermore, two algorithms, termed as MSD approximate ASD (MSDAASD) and MSD pruning ASD (MSDPASD), are proposed to reduce computational complexity and the number of comparisons, respectively. Compared with the existing typical algorithms, i.e., multiple symbol differential feedback detection (MS-DFD) and noncoherent sequence detection (NSD), the performance of the proposed algorithms is much superior to that of MS-DFD and a little inferior to that of NSD, while the complexity is lower than that of MS-DFD in most cases and significantly lower than that of NSD.展开更多
文摘[ Objective] This study aimed to investigate the effects of EMS (ethyl methane sulfonate) treatments on multiplication and differentiation of sugarcane embryonic cell clusters. [ Method] Sugarcane variety Xintaitang 22 (ROC22) was used as the experimental material. After treated with different concentrations of EMS for different time, sugarcane embryonic cell clusters were collected for subculture, differentiation and rooting, to compare and analyze the correlation of differ- ent EMS treatments with the muhiplicafion and differentiation of sugarcane embryonic cell clusters. [ Result] Treating ROC22 embryonic cell clusters with 0. 10% -0.15% EMS for4-6 h led to the best results, which reached the level of semi-lethal dose. Sugarcane embryonic cell clusters treated with EMS were adopted for subculture; results indicated that browning rate of cell clusters was higher than that in control (CK) but embryonic structure proportion was lower than that in control ; in addition, multiplication multiple of sugarcane embryonic cell clusters was also lower than that in control. After treated with EMS, sugarcane em- bryonic cell clusters exhibited significantly lower bud differentiation rate and higher browning rate compared with control. Furthermore, treating sugarcane embryonic cell clusters with 0.15% EMS for 2 h was conducive to plantlet emergence and rooting of sugarcane embryonic cell clusters. [ Conclusion] This study provided the- oretical basis for effective mutagenesis of sugarcane using EMS.
基金The Science Research Plan(Jijiaokehezi[2016]166)of Jilin Province Education Department During the 13th Five-Year Periodthe Science Research Starting Foundation(2015023)of Jilin Agricultural University
文摘In this paper, we study the existence of multiple positive periodic solutions for the second order differential equation x′′(t) + p(t)x′(t) + q(t)x(t) = f(t, x(t)).By using Krasnoselskii fixed point theorem, we establish some criteria for the existence and multiple positive periodic solutions for this differential equation.
基金Supported by the National Basic Research Program of China (973 Program) (Grant No. 2009CB320403)the National Defense Pre-researchProject of the 11th Five-Year-Plan of China (Grant No. 1060741001020102)
文摘Recently, a multiple symbol differential (MSD) sphere decoding (SD) algorithm for unitary spacetime modulation over quasi-static channel has been proved to achieve the performance of maximumlikelihood (ML) detection with relatively low complexity. However, an error floor occurs if the algorithm is applied over rapid-fading channels. Based on the assumption of continuous fading, a multiple symbol differential automatic sphere decoding (MSDASD) algorithm is developed by incorporating a recursive form of an ML metric into automatic SD (ASD) algorithm. Furthermore, two algorithms, termed as MSD approximate ASD (MSDAASD) and MSD pruning ASD (MSDPASD), are proposed to reduce computational complexity and the number of comparisons, respectively. Compared with the existing typical algorithms, i.e., multiple symbol differential feedback detection (MS-DFD) and noncoherent sequence detection (NSD), the performance of the proposed algorithms is much superior to that of MS-DFD and a little inferior to that of NSD, while the complexity is lower than that of MS-DFD in most cases and significantly lower than that of NSD.