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Isolating cDNA clones from rice induced by Magnaporthe grisea using PCR based differential screening method
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作者 DONG Haitao, DONG Jixin, WU Yuliang, HE Zuhua,and Li Debao,Biotechnology Institute,ZheJiang Agri Univ, Hangzhou 310029, China 《Chinese Rice Research Newsletter》 1998年第3期1-2,共2页
The differential hybridization technique hasbeen widely used to identify genes that are dif-ferentially expressed.However,this approachhas several drawbacks.First,the screeningprocedures are rather labor-intensive and... The differential hybridization technique hasbeen widely used to identify genes that are dif-ferentially expressed.However,this approachhas several drawbacks.First,the screeningprocedures are rather labor-intensive and time-consuming.Second,the amount of phageDNAs transferred onto the two filters may notbe equivalent,which leads to an inaccurate se-lection of a positive clone.Third,isolation ofphage DNA is slow and cumbersome.Here,aPCR based differential screening method that 展开更多
关键词 cDNA PCR Isolating cDNA clones from rice induced by Magnaporthe grisea using PCR based differential screening method
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Isolation, Expression Characteristics and Chromosomal Locations of Three cDNA Fragments Under Salt Stress in Rice 被引量:3
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作者 钱前 柳原城司 +3 位作者 滕胜 曾大力 朱立煌 陈受宜 《Acta Botanica Sinica》 CSCD 2003年第9期1090-1095,共6页
cDNA libraries were constructed from the leaves of a rice (Oryza sativa L.) salt tolerancevariety Tesan抋i 2 growing in solutions with 150 mmol/L NaCl for 3 h or without salt stress. Three salt-responsive cDNA clones,... cDNA libraries were constructed from the leaves of a rice (Oryza sativa L.) salt tolerancevariety Tesan抋i 2 growing in solutions with 150 mmol/L NaCl for 3 h or without salt stress. Three salt-responsive cDNA clones, Ts1, Ts2 and Ts3 were isolated by differential screening. Northern blottinganalysis showed that the transcription levels of Ts1 and Ts2 increased within 3 h salt stress and kept onincreasing within 24 h, while the transcription level of Ts3 reached its peak within 3 h. Sequence analysisindicated that there were no homologies between the three cDNA clones and any known gene. The threecDNA clones were mapped using a doubled haploid (DH) population derived from an indica variety ZYQ8,which was a salt tolerance parent of Tesan抋i 2, with a japonica variety JX17. Ts1, Ts2 and Ts3 werelocated on chromosomes 1, 3 and 7, respectively. It was noted that Ts1, Ts2, and Ts3 were in or near theregions of major or minor salt tolerance quantitative trait loci (QTLs), which were mapped in the same DHpopulation in a parallel study. 展开更多
关键词 RICE differential screening salt stress response mappingAbstract: cDNA libraries were constructed from the leaves of a rice (Oryza sativa L.) salttolerance variety Tesan’ai 2 growing in solutions with 150 mmol/L NaCl for 3 h or without s
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