"Beauty in Greatness Through Details:National Exhibition of Small Artworks"was held at the National Art Museum of China(NAMOC),which has the largest number of works exhibited at the museum in recent years.Th..."Beauty in Greatness Through Details:National Exhibition of Small Artworks"was held at the National Art Museum of China(NAMOC),which has the largest number of works exhibited at the museum in recent years.Throughout the art history,great masters both at home and abroad have world-renowned small artworks.展开更多
Using the method of ＂random sampling in typical colonies of the central area of the habitat＂ and several electrophoresis techniques, the variations of 17 structural loci encoding blood proteins in 60 Small-Tailed Ha...Using the method of ＂random sampling in typical colonies of the central area of the habitat＂ and several electrophoresis techniques, the variations of 17 structural loci encoding blood proteins in 60 Small-Tailed Han sheep and 73 Tan sheep were examined and compared with those of 14 other sheep populations in China and other countries to investigate their levels of genetic differentiation. The average heterozygosities of Small-Tailed Han sheep and Tan sheep were 0.2360 and 0.2587, respectively. The average polymorphic information content values were 0.1974 and 0.2102, respectively. The average effective numbers of alleles were 1.5723 and 1.5751, respectively. The coefficients of gene differentiation in the four groups （including 4, 6, 13, and 16 sheep populations, respectively） were 0.049323, 0.059987, 0.1728, and 0.201256, respectively, indicating that the degree of gene differentiation at the structural loci was the least in Hu sheep, Tong sheep, Small-Tailed Hart sheep, and Tan sheep; followed by the above-mentioned four sheep populations and two Mongolian sheep populations; and was the highest in sheep populations belonging to the Mongolian sheep group, South Asian sheep, and European sheep. The earlier researchers＇ conclusions that both Small-Tailed Han sheep and Tan sheep evolved from Mongolian sheep were further verified by the results of this study. Hu sheep, Tong sheep, Small-Tailed Han sheep, and Tan sheep were decreasingly affected by the bloodline of Mongolian sheep to different degrees. The relationships among sheep populations were not closely related to the geographical distances among sheep populations.展开更多
Small coastal pelagic fish are one of the fish families most affected by sea fishing. This man-made phenomenon leads to an imbalance in the marine and coastal ecosystem and is one of the main causes of migration north...Small coastal pelagic fish are one of the fish families most affected by sea fishing. This man-made phenomenon leads to an imbalance in the marine and coastal ecosystem and is one of the main causes of migration north and offshore of the ranges. We used the ordinary differential equations to model the interactions existing between small pelagic resources and fishermen. Modelling follows the same of the Lotka-Volterra equations with a difference in the number of variables. This study confirmed the instability of the marine ecosystem. The objective is first of all to model a system of three interacting individuals composed of two distinct types of predators and two types of prey, and then optimise this interaction with the aim of conserving biodiversity in the ecosystem under study. Determining the Jacobian matrix made it possible to calculate the reproduction rate basic (<em>R</em><sub>0</sub>). The study of the strong connectedness has made it possible to reduce the number of variables without losing the objective of the study. A computer program implemented on the language computer python facilitated the visualisation of the results.展开更多
Plasmablastic lymphoma(PBL) is a rare aggressive B-cell lymphoproliferative disorder,which has been characterized by the World Health Organization as a new entity.Although PBL is most commonly seen in the oral cavity ...Plasmablastic lymphoma(PBL) is a rare aggressive B-cell lymphoproliferative disorder,which has been characterized by the World Health Organization as a new entity.Although PBL is most commonly seen in the oral cavity of human immunodeficiency virus(HIV)-positive patients,it can also be seen in extraoral sites in immunocompromised patients who are HIV-negative.Here we present a rare case of PBL of the small intestine in a 55-year-old HIV-negative male.Histopathological examination of the excisional lesion showed a large cell lymphoma with plasmacytic differentiation diffusely infiltrating the small intestine and involving the surrounding organs.The neoplastic cells were diffusely positive for CD79a,CD138 and CD10 and partly positive for CD38 and epithelial membrane antigen.Approximately 80% of the tumor cells were positive for Ki-67.A monoclonal rearrangement of the kappa light chain gene was demonstrated.The patient died approximately 1.5 mo after diagnosis in spite of receiving two courses of the CHOP chemotherapy regimen.In a review of the literature,this is the first case report of PBL with initial presentation in the small intestine without HIV and Epstein-Barr virus infection,and a history of hepatitis B virus infection and radiotherapy probably led to the iatrogenic immunocompromised state.展开更多
This review aims to offer a vision of the clinical reality of cell therapy today in intensive medicine.For this,it has been carried out a description of the properties,functions,and Mesenchymal Stem Cells(MSCS)sources...This review aims to offer a vision of the clinical reality of cell therapy today in intensive medicine.For this,it has been carried out a description of the properties,functions,and Mesenchymal Stem Cells(MSCS)sources to subsequently address the evidence in preclinical models and studies clinical trials with whole cells and models attributed to small extracellular vesicles(sEVs),nanoparticles made up of microvesicles secreted by cells with an effect on the extracellular matrix,and their impact as an alternative towards cell-free regenerative medicine.MSCs are cells that enhance the regenerative capacity which can be differentiated typically in different lineages committed as bone,cartilage,and adipose tissue.On the other hand,small extracellular vesicles are structures that participate notoriously and crucially in intercellular communication,which has led to a change in the concept of the functions and the role that these vesicles play in living organisms,in the restoration of damaged tissues and the inflammatory response and immunological.We present the mechanisms that are involved in the applications of MSCS as whole cells and their sEVs in cell therapy and cell-free therapy as an alternative in regenerative medicine.Considering the structural loss that occurs after surgical procedures for cystic and tumoral pathology in periodontitis,as well as the maxillary atrophy that determines the rehabilitation with dental implants,it is imperative to find satisfactory solutions.The opportunity provided by the findings in stem cells is a recent introduction in the field of oral surgery,based on the regenerative potential that these cells possess to restore defects at different levels of the oral cavity.This review aims to discover the real applications that stem cells may have in our treatments shortly.展开更多
The transformation of Chengdu Tianfu New Area,a state-level new district,from the first site of constructing the“Park City”to the representative of the“Park City”,contains a unique path of“Chinese Style Governanc...The transformation of Chengdu Tianfu New Area,a state-level new district,from the first site of constructing the“Park City”to the representative of the“Park City”,contains a unique path of“Chinese Style Governance”.To expand the diverse paths of constructing megacities,the current research references the nudge theory of behavioral science,adopts the extended case method,and takes Chengdu Tianfu New Area as a typical case to summarize the“nudge”path of“Party City”construction.The study found that the government achieves the lock-in effect by the priming mechanism and uses the nudge strategy of improving environment and guiding public opinion,thereby helping the people make free choices.It realizes the framework effect by the salience mechanism and uses the strategies of highlighting key information and optimizing information delivery methods,which directly affects the behavior of the public.It achieves the commitment effect by the comparison mechanism,including the horizontal comparison and vertical comparison strategies,and gradually realizes the policy goals.It realizes the social norm effect by the imitation mechanism and uses the strategies of creating peer pressure and playing celebrity effect,which in turn influence people’s choices.With the help of the“nudging”mechanism,the construction of the park city has realized the interactive cycle of“government output-citizen feedback”and achieved the goal of“making great change through small actions”.As a new behavioral intervention tool,“nudging”provides an alternative explanation for the construction of the park city in Tianfu New Area and opens up new ideas for the construction of megacities.This study concludes that the construction of megacities can achieve the goal of“common return”through different ways and provides practical inspiration for future urban construction.展开更多
AIM: To investigate if loss of epithelial cell adhesion molecule (EpCAM) is associated with microinvasion in hepatocellular carcinomas (HCCs) in the presence of chronic hepatitis B.
To make the world a better place,it is necessary for us teenagers to take some small actions.As a saying goes,“The rose's in her hand,the flavor in mine.”On the one hand,we should be kind to others.We can take p...To make the world a better place,it is necessary for us teenagers to take some small actions.As a saying goes,“The rose's in her hand,the flavor in mine.”On the one hand,we should be kind to others.We can take part in volunteer activities.For example,we could volunteer to visit the old people's home to look after the old people.Because caring for old people is our Chinese tradition.We could help old people clean the room,wash the clothes,and read newspapers.We could also help to cheer up the sick kids in the hospital by telling stories and singing for them.I can get such a strong feeling of satisfaction when I see the kids get better and the look of joy on their faces.展开更多
For a preliminary study of the role of β-catenin/Tcf signaling in squamous differentiation of airway （tracheobronchial） epithelial cells, a stable mutant of β-catenin was transfected into primarily cultured porcin...For a preliminary study of the role of β-catenin/Tcf signaling in squamous differentiation of airway （tracheobronchial） epithelial cells, a stable mutant of β-catenin was transfected into primarily cultured porcine airway epithelial cells. Western blotting revealed that exogenous protein was observed in large quantity in cytoplasm and nucleus, When co-transfected with Tcf luciferase reporter plasmids, β-catenin mutant increased the reporter＇s transcriptional activities. However, mRNA expression of a squamous differentiation marker, small proline-rich protein （SPRP）, was not elevated, as shown by reverse transcription-polymerase chain reaction. These findings suggest that β-catenin/Tcf signaling may not be directly involved in the squamous differentiation of porcine airway epithelial cells.展开更多
With global warming now a certainty, it’s important to investigate climate change elements at the local level so as to enable stake holders adapt in order to sustain their livelihoods. This study investigated local c...With global warming now a certainty, it’s important to investigate climate change elements at the local level so as to enable stake holders adapt in order to sustain their livelihoods. This study investigated local climate changes, farmers’ perception of the changes and factors affecting perception to climate change in the Kyoga plains of Uganda. Monthly maximum temperature, minimum temperature and total rainfall from four meteorological stations within the study area for period 1984-2014 were obtained to analyse seasonal, annual and decadal trends in rainfall and temperature while a survey based on 384 randomly selected farmers was carried out to investigate the perception of variation in climate change trends among small holder farmers of different socioeconomic characteristics across the Kyoga plains. Multi stage random sampling was applied in the selection of the population sample. Non parametric analysis (Mann Kendall test) was used for analyzing trends and testing significance. In the survey, farmers were asked their observations about the local climate using structured questionnaires and these were analysed using descriptive statics. Logistics regression was then used to identify the factors that determined the perceptions of farmers on climate change. Overall, trends in monthly temperature are increasing over the years but not significantly while rainfall is decreasing but equally not significantly. Seasonal and decadal temperature had significant positive trends at different stations and sub zones over the years. 67% of the farmers realised a decrease in rainfall while 56.8% perceived an increase in temperature across the agroecological zone. 56.3% perceived declining rainfall and 52% realized increasing temperature in the southern sub zone while 42% realised a decrease in rainfall and 40.6%, an increase in temperature in the northern sub zone. Belonging to a group and age has significant positive effect on farmers’ perception of climate while farming experience and access to extension workers had a significant negative effect. The results suggest the need for strengthening networking among farmers for peer learning and support and location specific intervention measures to improve perception and adaptation to climate for each of the sub zones.展开更多
In order to reduce the regional differentials or narrow the gap in economic development between eastern and western areas and prevent the intensification of contradictions resulting from the regional inequality during...In order to reduce the regional differentials or narrow the gap in economic development between eastern and western areas and prevent the intensification of contradictions resulting from the regional inequality during the development process, the government of China launched the Great Campaign of Western Development （GCWD） in 2000. In this paper, we will evaluate the effects of the first 10 years＇ GCWD and use the latest Chinese annual data from 1978 to 2010 to investigate the problem of regional differentials in the development process, evaluate the effects of GCWD policy and test whether there is a regional Kuznets inverted-U curve in the regional development of China. The result shows that there are both good and bad news： the good news is that the inverted U-shaped curve of absolute differential between eastern and western areas finally emerges （the previous studies showed that the inverted-U curve did not appear and the differentials between eastern and western areas became worse） and the bad news is that the differential is in the first half of the inverted U-shaped curve in our choice of time span （1978-2010）, that is, it is still in the situation of deterioration, and the turning better point will emerge in 2025. Although the western areas have benefitted from GCWD, the absolute gap has been still widened. To narrow the gap, the western areas should accelerate the industrial development and the government should adjust the policy and make more efforts to support the development of the industry in the western areas, especially cultivate and support the development of the western characteristic and advantageous industry rather than just supporting the construction of infrastructure in the western areas.展开更多
To examine the effect of myogenin gene on the differentiation of bovine skeletal muscle satellite cell, we constructed small interfering RNA plasmid vector to obtain myogenin knockdown bovine skeletal muscle cells, th...To examine the effect of myogenin gene on the differentiation of bovine skeletal muscle satellite cell, we constructed small interfering RNA plasmid vector to obtain myogenin knockdown bovine skeletal muscle cells, then used cell transfection, real time RCR and Western Blot to detect the influence of myogenin to cell differentiation. Results showed that the knockdown of myogenin significantly decreased its expression and other muscle-specific genes. Compared to the control, it could differentiate into few myotubes when challenged by low serum in the medium. These findings provided an important theoretical basis for further explore of the genetic mechanism in adult skeletal muscle, the remedy of muscle injuries and the cultivation of high-yield transgenic cattle.展开更多
Editor's note:From August 2019,mobilized by the Youth League Committee directly under the Ministry of Culture and Tourism of the People's Republic of China,young cadres under the age of 40 have made surveys on...Editor's note:From August 2019,mobilized by the Youth League Committee directly under the Ministry of Culture and Tourism of the People's Republic of China,young cadres under the age of 40 have made surveys on the theme of"practice of integrated development of culture and tourism at the grassroots lever"during holidays.展开更多
文摘"Beauty in Greatness Through Details:National Exhibition of Small Artworks"was held at the National Art Museum of China(NAMOC),which has the largest number of works exhibited at the museum in recent years.Throughout the art history,great masters both at home and abroad have world-renowned small artworks.
基金This paper is translated from its Chinese version in Scientia Agricultura Sinica.We thank the anonymous reviewers for their comments on this manuscript.This study was supported by the International Cooperation Project of the National Natural Science Foundation of China(30310103007 and 30410103150)Project of the Basic Natural Science Foundation for Colleges and Universities in Jiangsu Province,China(06KJD230203).
文摘Using the method of ＂random sampling in typical colonies of the central area of the habitat＂ and several electrophoresis techniques, the variations of 17 structural loci encoding blood proteins in 60 Small-Tailed Han sheep and 73 Tan sheep were examined and compared with those of 14 other sheep populations in China and other countries to investigate their levels of genetic differentiation. The average heterozygosities of Small-Tailed Han sheep and Tan sheep were 0.2360 and 0.2587, respectively. The average polymorphic information content values were 0.1974 and 0.2102, respectively. The average effective numbers of alleles were 1.5723 and 1.5751, respectively. The coefficients of gene differentiation in the four groups （including 4, 6, 13, and 16 sheep populations, respectively） were 0.049323, 0.059987, 0.1728, and 0.201256, respectively, indicating that the degree of gene differentiation at the structural loci was the least in Hu sheep, Tong sheep, Small-Tailed Hart sheep, and Tan sheep; followed by the above-mentioned four sheep populations and two Mongolian sheep populations; and was the highest in sheep populations belonging to the Mongolian sheep group, South Asian sheep, and European sheep. The earlier researchers＇ conclusions that both Small-Tailed Han sheep and Tan sheep evolved from Mongolian sheep were further verified by the results of this study. Hu sheep, Tong sheep, Small-Tailed Han sheep, and Tan sheep were decreasingly affected by the bloodline of Mongolian sheep to different degrees. The relationships among sheep populations were not closely related to the geographical distances among sheep populations.
文摘Small coastal pelagic fish are one of the fish families most affected by sea fishing. This man-made phenomenon leads to an imbalance in the marine and coastal ecosystem and is one of the main causes of migration north and offshore of the ranges. We used the ordinary differential equations to model the interactions existing between small pelagic resources and fishermen. Modelling follows the same of the Lotka-Volterra equations with a difference in the number of variables. This study confirmed the instability of the marine ecosystem. The objective is first of all to model a system of three interacting individuals composed of two distinct types of predators and two types of prey, and then optimise this interaction with the aim of conserving biodiversity in the ecosystem under study. Determining the Jacobian matrix made it possible to calculate the reproduction rate basic (<em>R</em><sub>0</sub>). The study of the strong connectedness has made it possible to reduce the number of variables without losing the objective of the study. A computer program implemented on the language computer python facilitated the visualisation of the results.
文摘Plasmablastic lymphoma(PBL) is a rare aggressive B-cell lymphoproliferative disorder,which has been characterized by the World Health Organization as a new entity.Although PBL is most commonly seen in the oral cavity of human immunodeficiency virus(HIV)-positive patients,it can also be seen in extraoral sites in immunocompromised patients who are HIV-negative.Here we present a rare case of PBL of the small intestine in a 55-year-old HIV-negative male.Histopathological examination of the excisional lesion showed a large cell lymphoma with plasmacytic differentiation diffusely infiltrating the small intestine and involving the surrounding organs.The neoplastic cells were diffusely positive for CD79a,CD138 and CD10 and partly positive for CD38 and epithelial membrane antigen.Approximately 80% of the tumor cells were positive for Ki-67.A monoclonal rearrangement of the kappa light chain gene was demonstrated.The patient died approximately 1.5 mo after diagnosis in spite of receiving two courses of the CHOP chemotherapy regimen.In a review of the literature,this is the first case report of PBL with initial presentation in the small intestine without HIV and Epstein-Barr virus infection,and a history of hepatitis B virus infection and radiotherapy probably led to the iatrogenic immunocompromised state.
文摘This review aims to offer a vision of the clinical reality of cell therapy today in intensive medicine.For this,it has been carried out a description of the properties,functions,and Mesenchymal Stem Cells(MSCS)sources to subsequently address the evidence in preclinical models and studies clinical trials with whole cells and models attributed to small extracellular vesicles(sEVs),nanoparticles made up of microvesicles secreted by cells with an effect on the extracellular matrix,and their impact as an alternative towards cell-free regenerative medicine.MSCs are cells that enhance the regenerative capacity which can be differentiated typically in different lineages committed as bone,cartilage,and adipose tissue.On the other hand,small extracellular vesicles are structures that participate notoriously and crucially in intercellular communication,which has led to a change in the concept of the functions and the role that these vesicles play in living organisms,in the restoration of damaged tissues and the inflammatory response and immunological.We present the mechanisms that are involved in the applications of MSCS as whole cells and their sEVs in cell therapy and cell-free therapy as an alternative in regenerative medicine.Considering the structural loss that occurs after surgical procedures for cystic and tumoral pathology in periodontitis,as well as the maxillary atrophy that determines the rehabilitation with dental implants,it is imperative to find satisfactory solutions.The opportunity provided by the findings in stem cells is a recent introduction in the field of oral surgery,based on the regenerative potential that these cells possess to restore defects at different levels of the oral cavity.This review aims to discover the real applications that stem cells may have in our treatments shortly.
基金supported by General Project of National Social Science Fund[Grant number.22BZZ077],Study on Efficiency Improvement Mechanism of Rural Relative Poverty Governance Driven by Big Data.
文摘The transformation of Chengdu Tianfu New Area,a state-level new district,from the first site of constructing the“Park City”to the representative of the“Park City”,contains a unique path of“Chinese Style Governance”.To expand the diverse paths of constructing megacities,the current research references the nudge theory of behavioral science,adopts the extended case method,and takes Chengdu Tianfu New Area as a typical case to summarize the“nudge”path of“Party City”construction.The study found that the government achieves the lock-in effect by the priming mechanism and uses the nudge strategy of improving environment and guiding public opinion,thereby helping the people make free choices.It realizes the framework effect by the salience mechanism and uses the strategies of highlighting key information and optimizing information delivery methods,which directly affects the behavior of the public.It achieves the commitment effect by the comparison mechanism,including the horizontal comparison and vertical comparison strategies,and gradually realizes the policy goals.It realizes the social norm effect by the imitation mechanism and uses the strategies of creating peer pressure and playing celebrity effect,which in turn influence people’s choices.With the help of the“nudging”mechanism,the construction of the park city has realized the interactive cycle of“government output-citizen feedback”and achieved the goal of“making great change through small actions”.As a new behavioral intervention tool,“nudging”provides an alternative explanation for the construction of the park city in Tianfu New Area and opens up new ideas for the construction of megacities.This study concludes that the construction of megacities can achieve the goal of“common return”through different ways and provides practical inspiration for future urban construction.
基金Supported by Key Project of Tianjin Science and Technology Committee,No.05YFSZSF02500Foundation of Tianjin,No.08JCYBJC08300Key Research Project of Tianjin Healthy Bureau,No.11KG112
文摘AIM: To investigate if loss of epithelial cell adhesion molecule (EpCAM) is associated with microinvasion in hepatocellular carcinomas (HCCs) in the presence of chronic hepatitis B.
文摘To make the world a better place,it is necessary for us teenagers to take some small actions.As a saying goes,“The rose's in her hand,the flavor in mine.”On the one hand,we should be kind to others.We can take part in volunteer activities.For example,we could volunteer to visit the old people's home to look after the old people.Because caring for old people is our Chinese tradition.We could help old people clean the room,wash the clothes,and read newspapers.We could also help to cheer up the sick kids in the hospital by telling stories and singing for them.I can get such a strong feeling of satisfaction when I see the kids get better and the look of joy on their faces.
基金a grant from the National Natural Sciences Foundation of China (No.30200115)
文摘For a preliminary study of the role of β-catenin/Tcf signaling in squamous differentiation of airway （tracheobronchial） epithelial cells, a stable mutant of β-catenin was transfected into primarily cultured porcine airway epithelial cells. Western blotting revealed that exogenous protein was observed in large quantity in cytoplasm and nucleus, When co-transfected with Tcf luciferase reporter plasmids, β-catenin mutant increased the reporter＇s transcriptional activities. However, mRNA expression of a squamous differentiation marker, small proline-rich protein （SPRP）, was not elevated, as shown by reverse transcription-polymerase chain reaction. These findings suggest that β-catenin/Tcf signaling may not be directly involved in the squamous differentiation of porcine airway epithelial cells.
文摘With global warming now a certainty, it’s important to investigate climate change elements at the local level so as to enable stake holders adapt in order to sustain their livelihoods. This study investigated local climate changes, farmers’ perception of the changes and factors affecting perception to climate change in the Kyoga plains of Uganda. Monthly maximum temperature, minimum temperature and total rainfall from four meteorological stations within the study area for period 1984-2014 were obtained to analyse seasonal, annual and decadal trends in rainfall and temperature while a survey based on 384 randomly selected farmers was carried out to investigate the perception of variation in climate change trends among small holder farmers of different socioeconomic characteristics across the Kyoga plains. Multi stage random sampling was applied in the selection of the population sample. Non parametric analysis (Mann Kendall test) was used for analyzing trends and testing significance. In the survey, farmers were asked their observations about the local climate using structured questionnaires and these were analysed using descriptive statics. Logistics regression was then used to identify the factors that determined the perceptions of farmers on climate change. Overall, trends in monthly temperature are increasing over the years but not significantly while rainfall is decreasing but equally not significantly. Seasonal and decadal temperature had significant positive trends at different stations and sub zones over the years. 67% of the farmers realised a decrease in rainfall while 56.8% perceived an increase in temperature across the agroecological zone. 56.3% perceived declining rainfall and 52% realized increasing temperature in the southern sub zone while 42% realised a decrease in rainfall and 40.6%, an increase in temperature in the northern sub zone. Belonging to a group and age has significant positive effect on farmers’ perception of climate while farming experience and access to extension workers had a significant negative effect. The results suggest the need for strengthening networking among farmers for peer learning and support and location specific intervention measures to improve perception and adaptation to climate for each of the sub zones.
文摘In order to reduce the regional differentials or narrow the gap in economic development between eastern and western areas and prevent the intensification of contradictions resulting from the regional inequality during the development process, the government of China launched the Great Campaign of Western Development （GCWD） in 2000. In this paper, we will evaluate the effects of the first 10 years＇ GCWD and use the latest Chinese annual data from 1978 to 2010 to investigate the problem of regional differentials in the development process, evaluate the effects of GCWD policy and test whether there is a regional Kuznets inverted-U curve in the regional development of China. The result shows that there are both good and bad news： the good news is that the inverted U-shaped curve of absolute differential between eastern and western areas finally emerges （the previous studies showed that the inverted-U curve did not appear and the differentials between eastern and western areas became worse） and the bad news is that the differential is in the first half of the inverted U-shaped curve in our choice of time span （1978-2010）, that is, it is still in the situation of deterioration, and the turning better point will emerge in 2025. Although the western areas have benefitted from GCWD, the absolute gap has been still widened. To narrow the gap, the western areas should accelerate the industrial development and the government should adjust the policy and make more efforts to support the development of the industry in the western areas, especially cultivate and support the development of the western characteristic and advantageous industry rather than just supporting the construction of infrastructure in the western areas.
基金Supported by the Ministry of Agricultural Nuarture of New Varieties Genetically Modified Organisms Significant Special Funding (2008ZX08007-002)
文摘To examine the effect of myogenin gene on the differentiation of bovine skeletal muscle satellite cell, we constructed small interfering RNA plasmid vector to obtain myogenin knockdown bovine skeletal muscle cells, then used cell transfection, real time RCR and Western Blot to detect the influence of myogenin to cell differentiation. Results showed that the knockdown of myogenin significantly decreased its expression and other muscle-specific genes. Compared to the control, it could differentiate into few myotubes when challenged by low serum in the medium. These findings provided an important theoretical basis for further explore of the genetic mechanism in adult skeletal muscle, the remedy of muscle injuries and the cultivation of high-yield transgenic cattle.
文摘Editor's note:From August 2019,mobilized by the Youth League Committee directly under the Ministry of Culture and Tourism of the People's Republic of China,young cadres under the age of 40 have made surveys on the theme of"practice of integrated development of culture and tourism at the grassroots lever"during holidays.
