In vitro growth, differentiation and biological characteristics of neural stem cells

OBJECTIVE： To summarize the biological characteristics of neural stem cells, and the separation, purification. differentiation and source of neural stem cells. DATA SOURCES ： An online search of Pubmed database was undertaken to identify English articles about the growth of neural stem cells in vitro published from January 2000 to October 2006 by using the keywords of ＂neural stem cells, bone marrow mesenchymal stem cells （BMSCs）, umbilical cord blood stem cells, embryonic stem cells （ESC）, separation methods, neural growth factor＂. And relevant articles published in IEEE/IEE Electronic Library （IEL） database, Springer Link database and Kluwer Online Journals were also searched, Chinese relevant articles published between January 2000 to October 2006 were searched with the same keywords in Chinese in Chinese journal full-text database. STUDY SELECTION ： The articles were primarily screened, and then the full-texts were searched. Inclusive criteria： （1） Articles relevant to the biological characteristics and classification of neural stem cells; （2） Articles about the source, separation and differentiation of the ESCs, BMSCs and umbilical cord blood stem cells. The repetitive studies and reviews were excluded. DATA EXTRACTION ： Thirty articles were selected from 203 relevant articles according to the inclusive criteria Articles were excluded because of repetition and reviews. DATA SYNTHESES ： Neural stem cells have the ability of self-renewing and high differentiation, and they are obtained from ESCs, nerve tissue, nerve system, BMSCs and umbilical cord blood stem cells. ESCs can be separated by means of mechanical dissociation is better than that of the trypsin digestion, BMSCs by density gradient centrifuge separation, hemolysis, whole-blood culture, etc., and umbilical cord blood stem ceils by Ficoil density gradient centrifugation, hydroxyethyl starch （HES） centrifugation sedimentation, etc. Neural growth factor （NGF） and other factors play an important role in the growth of NSCs, such as transforming growth factor （TGF） is an important player in repairing organs, NGF accelerates the process of growth, insulin-like growth factor serves importantly in the differentiation of stem cells into neuron-like cells. CONCLUSION ： As unipotent stem cells, NSCs have the abilities of self-renewal and potential of high differentiation. The method of mechanical dissociation is better than trypsin digestion in e separating ESCs. However, density gradient centrifuge separation is better than other methods in the separation of the BMSCs. NGF and other factors play an important role in the growth of NSCs.

Characteristics of highly differentiated granite and metallization of tungsten-tin, rare and rare earth metal in the eastern Nanling region, China

1 Introduction Massive tungsten-tin,rare and rare earth metals ore deposits were formed with the widespread granite magmatic activity in early Yanshanian period in the eastern Nanling region.Recent studies indicate that the Yanshanian highly differentiated-granite formation is closely related to the deposits of tungsten and tin,rare and rare earth metals mineralization in the region(Xiao

CHARACTERISTICS OF NATURAL CONTENTS OF CHEMICAL ELEMENTS IN SOILS OF KARAKORUM-WEST KUNLUN MOUNTAIN REGION

Natural contents of 23 elements in soils of Karakorum-West Kunlun Mts are studied. By comparison of natural contents andcalculation of regional differentiation coefficients, similarity anddchrence of elemental contents between soils of the region and thatof China and of the world are discussed. Through main componentanalysis, relation of intergrowth and realignment among elementsin soils and characteristics of cluster expressed in soil types are alsodiscussed. Coherence of elements in soi1s and characteristics ofregional dtherence of the elements are clarified. The concernedregion is abound in natural resources. Research on natural contentsof chemical elements in soils of the region is significant forstudying elemental chemical geography and environmentalgeochemistry. Besides, it also offers necessary data for explorationand development of this region.

County scale spatial differential characteristics of cultivated land use grade: A case of Binchuan County

Land resource is the material foundation of human survival and economic development, and the cultivated land is the essence of land resources. This paper takes the county scale, by using the method of GIS spatial analysis and statistical analysis unifies, to explore the quality of cultivated land in Binchuan County using level, and explain the utilization level of county cultivated land quality spatial differentiation characteristics. The results showed that:(1) in the quantity of cultivated land quality and utilization level, the average utilization of paddy land was greater than that of dry land, and the comprehensive utilization of cultivated land and so on. Among them, the paddy land ranged from grade 6 to 15, the average utilization was grade 11.6; dry land ranged from grade 2 to 11, the average utilization was grade 5.9; comprehensive range of cultivated land is grade 2 to 15, the average utilization was grade 8.1;(2) the quality of cultivated land utilization spatial differentiation. Paddy field, dry land and cultivated land and large value distribution in the central and southern, inverted V shape distribution; the smaller the value distribution in the East and West, a dumbbell shaped distribution. Among them, they don't use a larger value of paddy land distributed in the central, South and southwest, the maximum value is 14.3, the smaller the value distribution in the West and East, the minimum value is grade 7; the larger the value distribution by the dry land in the South and West, the maximum value is grade 10.2, the smaller the value distribution in the West and East. The minimum value is grade 2.3; the comprehensive utilization of cultivated land don't distributed larger value in the central, South and south-west, the maximum value is grade 12.7, the smaller the value distribution in the West and East, the minimum value is grade 3.5. This paper can provide scientific basis for the dynamic monitoring of cultivated land quality, the transformation of low yield farmland and the early warning of cultivated land pressure.

Morphological characteristics and DNA barcoding of Pampus echinogaster(Basilewsky, 1855)

The morphological similarities of Pampus fishes have led to considerable confusion in species-level identification,and no accurate information on neotype or DNA barcoding of Pampus echinogaster is available. Two hundred and seven specimens of P. echinogaster were collected from the coastal waters of Dandong, Dongying, Qingdao,Nantong, Zhoushan, Wenzhou, Changle, Taiwan, and Wakayama（Japan）, from June 2010 to April 2013. The diagnostic characteristics of P. echinogaster are as follows： dorsal fin VIII-XI-43–51, anal fin V-VIII-43–49, pectoral fin 22–27, caudal fin 19–22, pelvic fin absent; first gill rakers sparse, slender（pointed）, 3–4＋12–16=15–20; vertebrae39–41; transverse occipital canal on top of head moderately small, wavy ridges not reaching upper origin of pectoral fin; ventral branch of lateral line canal spare, shorter than dorsal branch of lateral line canal. By combining congener sequences of the cytochrome oxidase I（COI） gene from Gen Bank, two absolute groups were detected among all specimens, which further indicated that two valid species were present based on genetic differences in amino acid sequences and the distance between the groups. The sequences of Group 1 can be regarded as DNA barcoding of P. echinogaster. The correct morphological redescription and DNA barcoding of P.echinogaster are presented here to provide a guarantee for efficient and accurate studies, a theoretical basis for classification, and enable appropriate fishery management and conservation strategies for the genus Pampus in the future.

Organic matter pores in the chang 7 lacustrine shales from the Ordos Basin and its effect on reflectance measurement

To quantify the pore characteristics of various macerals in Chang 7 lacustrine shales,macerals were effectively identified according to their optical and morphological characteristics,and the nanoscale pore structure of macerals was observed by scanning electron microscope.Meanwhile,the reflectances of different positions in the same pieces of vitrinite or solid bitumen with heterogeneous pores development were measured.The results showed that the average contents of sapropelinite,liptinite,vitrinite,inertinite and solid bitumen are 42.7%,8.7%,13.6%,13.8% and 21.2%,respectively,which suggests that the source of the organic matter of the Chang 7 shales is a mixed source input.The organic pores of Chang 7 shales are enriched,and the pore shapes are mostly round or elliptical.The pore size of organic pores has a wide distribution,mainly concentrate in the range of 100-400 nm,and the average plane porosity of organic pores is 10.13%.The size order of the organic pores in various macerals is:solid bitumen<bituminite<alginite<vitrinite<fusinite<liptinite.The abundance order of organic matter pores of each maceral is as follows:alginite>fusinite>bituminite>solid bitumen>vitrinite>liptinite.OM pores are mainly contributed by bituminite,solid bitumen and fusinite.The plane porosity of bituminite increases with maturity.In the process of thermal evolution,the plane porosity of fusinite is distributed in the two ranges of 20%-28% and 1%-7%.The former is mainly the primary pores of the fusinite itself,and the latter is the secondary pores formed in the thermal evolution.As for the organic pores of other macerals,no obvious thermal evolution law was found.Meanwhile,the surface imperfections of vitrinite or solid bitumen is enhanced by the enrichment of organic pores(an increase in pore size or pore number),which may result in the underestimation of their reflectances.

Conservation Laws of a Class of Combined Equations

In this paper, we investigate conservation laws of a class of partial differential equations, which combines the nonlinear telegraph equations and the nonlinear diffusion-convection equations. Moreover, some special conservation laws of the combined equations are obtained by means of symmetry classifications of wave equations uxx = H （x）utt.

Differential characteristic set algorithm for the complete symmetry classification of partial differential equations

In this paper, we present a differential polynomial characteristic set algorithm for the complete symmetry classification of partial differential equations （PDEs） with some parameters. It can make the solution to the complete symmetry classification problem for PDEs become direct and systematic. As an illustrative example, the complete potential symmetry classifications of nonlinear and linear wave equations with an arbitrary function parameter are presented. This is a new application of the differential form characteristic set algorithm, i.e., Wu＇s method, in differential equations.

Development of Wages and Salaries of Czech Employees During the Last Period by Educational Attainment and Forecasts for 2011 and 2012

This paper deals with the development of sample characteristics of wage distribution in recent years in the Czech Republic by the highest educational attainment. Gross monthly wage is the variable investigated. We distinguish the following scale of the highest educational attainment： primary and incomplete education, secondary education without GCSE, secondary education with GCSE, higher vocational and bachelor education and tertiary education. Forecasts of wage distribution have been developed for the next two years for all of these categories. Three-parametric lognormal curve formed the basis of the theoretical probability distribution. Parameter values of relevant three-parametric lognormal curves were then estimated using the method of L-moments of parameter estimation. Forecasts of sample values of L-moments were calculated using trend analysis of their past development and the parameters of three-parametric Iognormal curves for forecasts of wage distribution were calculated using the predicted values of the first three sample L-moments. We have obtained the forecasts of wage distribution by the highest educational attainment on the basis of these probability density functions.

Introduction to an Invariant Quantity Method

It is impossible,mathematically, to use a time series which is regarded as a set of observational facts of a dynamicsystem to reconstruct the particular system.Physically, however, with a few assumptions put, a dynamic system canbe rebuilt approximately by means of observational facts.This is the goal of the so called invariant quantity method(IQM),whose research and experiment are of potential significance to atmospheric sciences.

Searching for impossible subspace trails and improved impossible differential characteristics for SIMON-like block ciphers

In this paper,we greatly increase the number of impossible differentials for SIMON and SIMECK by eliminating the 1-bit constraint in input/output difference,which is the precondition to ameliorate the complexity of attacks.We propose an algorithm which can greatly reduce the searching complexity to find such trails efficiently since the search space exponentially expands to find impossible differentials with multiple active bits.There is another situation leading to the contradiction in impossible differentials except for miss-in-the-middle.We show how the contradiction happens and conclude the precondition of it defined as miss-from-the-middle.It makes our results more comprehensive by applying these two approach simultaneously.This paper gives for the first time impossible differential characteristics with multiple active bits for SIMON and SIMECK,leading to a great increase in the number.The results can be verified not only by covering the state-of-art,but also by the MILP model.

An attack on hash function HAVAL-128

Abstract In this paper, we give a fast attack against hash function HAVAL-128. HAVAL was presented by Y. L. Zheng et al. at Auscrypto＇92. It can be processed in 3, 4 or 5 passes, and produces 128, 160, 192, or 224-bit fingerprint. We break the HAVAL with 128-bit fingerprint. The conclusion is that, given any 1024-bit message ra, we just make some modifications about m, and the modified message ra can collide with another message m only with probability 1/2^7, where m = m ＋ △m, in which △m is a fixed difference selected in advance. In addition, two collision examples for HAVAL-128 are given in this paper.