Specific cell subpopulations identified as cancer stem cells(CSCs)can be found in basal cell carcinoma(BCC).Generally,CSCs have a marked trans-differentiation potential that could potentially be used in differentiatio...Specific cell subpopulations identified as cancer stem cells(CSCs)can be found in basal cell carcinoma(BCC).Generally,CSCs have a marked trans-differentiation potential that could potentially be used in differentiation therapies.However,there are no studies regarding BCC CSCs multipotency.The aim of the study was to analyze the characteristic of CSCs of BCC with emphasis on their differentiation potential upon specific induction.Specific staining and cell morphology were used for differentiation confirmation,along with the expression analysis of osteogenic(ALP,BSP,Runx2,OCN,BMP2),chondrogenic(COL1 and COL2A1),adipogenic(PPAR-γ)and neurogenic(Nestin and MAP2)markers.BCC CSCs differentiated into osteogenic and chondrogenic lineages,as judged by staining and high expression of specific markers(from 2-to 92-fold higher upon induction).Concomitantly with differentiation,the levels of cancer stem cell markers decreased in the cultures.Adipo-differentiation and neuro-differentiation were unsuccessful.In conclusion,BCC CSCs exhibit the capacity to trans-differentiate,a characteristic that may potentially be useful in the development of new strategies for the treatment of aggressive BCCs.展开更多
Recent advances in stem cell technologies have opened new avenues for the treatment of a number of diseases still lacking effective therapeutic options.Cell transplantation has emerged as among the most promising clin...Recent advances in stem cell technologies have opened new avenues for the treatment of a number of diseases still lacking effective therapeutic options.Cell transplantation has emerged as among the most promising clinical intervention for disorders such as injuries,diabetes,liver diseases, neurodegeneration and heart failure (Lee et al., 2013; Forbes and Rosenthal, 2014; Tabar and Studer, 2014).展开更多
This study was designed to verify the stem cell properties of sheep amniotic epithelial cells and their capacity for neural differentiation. Immunofluorescence microscopy and reverse transcription-PCR revealed that th...This study was designed to verify the stem cell properties of sheep amniotic epithelial cells and their capacity for neural differentiation. Immunofluorescence microscopy and reverse transcription-PCR revealed that the sheep amniotic epithelial cells were positive for the embryonic stem cell marker proteins SSEA-1, SSEA-3, SSEA-4, TRA-1-60 and TRA-1-81, and the totipotency-associated genes Oct-4, Sox-2 and Rex-1, but negative for Nanog. Amniotic epithelial cells expressed β-Ⅲ-tubulin, glial fibrillary acidic protein, nestin and microtubule-associated protein-2 at 28 days after induction with serum-free neurobasal-A medium containing B-27. Thus, sheep amniotic epithelial cells could differentiate into neurons expressing β-Ⅲ-tubulin and microtubule-associated protein-2, and glial-like cells expressing glial fibrillary acidic protein, under specific conditions.展开更多
In the present study, we used a proteomics approach based on a two-dimensional electrophoresis (2-DE) reference map to investigate protein expression in the ovarian tissues of pubertal Swiss-Webster mice subjected t...In the present study, we used a proteomics approach based on a two-dimensional electrophoresis (2-DE) reference map to investigate protein expression in the ovarian tissues of pubertal Swiss-Webster mice subjected to carbon ion radiation (CIR). Among the identified proteins, ubiquitin carboxy-terminal hydrolase L1 (UCH-L1) is associated with the cell cycle[1] and that it influences proliferation in ovarian tissues. We analyzed the expression of UCH-L1 and the proliferation marker proliferation cell nuclear antigen (PCNA) following CIR using immunoblotting and immunofluorescence. The proteomics and biochemical results provide insight into the underlying mechanisms of CIR toxicity in ovarian tissues.展开更多
Industrial wireless mesh networks(WMNs)have been widely deployed in various industrial sectors,providing services such as manufacturing process monitoring,equipment control,and sensor data collection.A notable charact...Industrial wireless mesh networks(WMNs)have been widely deployed in various industrial sectors,providing services such as manufacturing process monitoring,equipment control,and sensor data collection.A notable characteristic of industrial WMNs is their distinct traffic pattern,where the majority of traffic flows originate from mesh nodes and are directed towards mesh gateways.In this context,this paper adopts and revisits a routing algorithm known as ALFA(autonomous load-balancing field-based anycast routing),tailored specifically for anycast(one-to-one-of-many)networking in WMNs,where traffic flows can be served through any one of multiple gateways.In essence,the scheme is a hybrid-type routing strategy that leverages the advantages of both back-pressure routing and geographic routing.Notably,its novelty lies in being developed by drawing inspiration from another field,specifically from the movement of charges in an electrostatic potential field.Expanding on the previous work,this paper explores further in-depth discussions that were not previously described,including a detailed description of the analogy between an electrostatic system and a WMN system based on precise mapping perspectives derived from intensive analysis,as well as discussions on anycast,numerical methods employed in devising the ALFA scheme,its characteristics,and complexity.It is worth noting that this paper addresses these previously unexplored aspects,representing significant contributions compared to previous works.As a completely new exploration,a new scheduling strategy is proposed that is compatible with the routing approach by utilizing the potential-based metric not only in routing but also in scheduling.This assigns higher medium access priority to links with a larger potential difference.Extensive simulation results demonstrate the superior performance of the proposed potential-based joint routing and scheduling scheme across various aspects within industrial WMN scenarios.展开更多
Mesenchymal stem cells(MSCs)have attracted great attention as a source of cells for regenerative medicine owing to their self-renewal ability and multiple differentiation potential.However,the serious shortage of MSCs...Mesenchymal stem cells(MSCs)have attracted great attention as a source of cells for regenerative medicine owing to their self-renewal ability and multiple differentiation potential.However,the serious shortage of MSCs and the difficulty of maintaining the differentiation potential during culture in vitro limit their application.Microcarrier culture technology is an effective method to realize large-scale culture of MSCs,whereas the effect of microcarriers properties on MSCs proliferation and differentiation potential maintenance should be investigated.In this study,Konjac glucomannan(KGM)microcarriers with a wide range of rigidity from 0.2 MPa to 10 MPa were prepared using water-in-oil emulsion polymerization.It was found that the microcarriers rigidity had great influence on MSCs by regulating cell spreading rate.Rapid proliferation and good differentiation potential maintenance of MSCs were achieved on the intermediate rigidity about 2.51±0.65 MPa with the faster spreading rate of cells.As a result,MSCs on 2.51±0.65 MPa KGM microcarriers proliferated about 27 times,which was 1.7 times as much as MSCs cultured on the commercial microcarriers Cytodex-1.The differentiation potential was also improved about 3.2 times compared to Cytodex-1.Therefore,it is indispensable to regulate microcarriers rigidity,especially for application of MSCs.展开更多
The motion planning problem for multi-agent systems becomes particularly challenging when humans or human-controlled robots are present in a mixed environment.To address this challenge,this paper presents an interacti...The motion planning problem for multi-agent systems becomes particularly challenging when humans or human-controlled robots are present in a mixed environment.To address this challenge,this paper presents an interaction-aware motion planning approach based on game theory in a receding-horizon manner Leveraging the framework provided by dynamic potential games for handling the interactions among agents,this approach formulates the multi-agent motion planning problem as a differential potential game,highlighting the effectiveness of constrained potential games in facilitating interactive motion planning among agents.Furthermore,online learning techniques are incorporated to dynamically learn the unknown preferences and models of humans or human-controlled robots through the analysis of observed data.To evaluate the effectiveness of the proposed approach,numerical simulations are conducted,demonstrating its capability to generate interactive trajectories for all agents,including humans and human-controlled agents,operating within the mixed environment.The simulation results illustrate the effectiveness of the proposed approach in handling the complexities of multi-agent motion planning in real-world scenarios.展开更多
基金the Ministry of Education,Science and Technological Development,Republic of Serbia(Grant No.451-03-9/2021-14/200129).
文摘Specific cell subpopulations identified as cancer stem cells(CSCs)can be found in basal cell carcinoma(BCC).Generally,CSCs have a marked trans-differentiation potential that could potentially be used in differentiation therapies.However,there are no studies regarding BCC CSCs multipotency.The aim of the study was to analyze the characteristic of CSCs of BCC with emphasis on their differentiation potential upon specific induction.Specific staining and cell morphology were used for differentiation confirmation,along with the expression analysis of osteogenic(ALP,BSP,Runx2,OCN,BMP2),chondrogenic(COL1 and COL2A1),adipogenic(PPAR-γ)and neurogenic(Nestin and MAP2)markers.BCC CSCs differentiated into osteogenic and chondrogenic lineages,as judged by staining and high expression of specific markers(from 2-to 92-fold higher upon induction).Concomitantly with differentiation,the levels of cancer stem cell markers decreased in the cultures.Adipo-differentiation and neuro-differentiation were unsuccessful.In conclusion,BCC CSCs exhibit the capacity to trans-differentiate,a characteristic that may potentially be useful in the development of new strategies for the treatment of aggressive BCCs.
基金supported by Fondation pour la Recherche Médicale(Equipe FRM),SATT Sud Est-Accelerator of Technology Transfer,Association France Parkinson,Fondation de France(Committee Parkinson),COST Action CM1106
文摘Recent advances in stem cell technologies have opened new avenues for the treatment of a number of diseases still lacking effective therapeutic options.Cell transplantation has emerged as among the most promising clinical intervention for disorders such as injuries,diabetes,liver diseases, neurodegeneration and heart failure (Lee et al., 2013; Forbes and Rosenthal, 2014; Tabar and Studer, 2014).
基金funded by the National High-Tech Research and Development Program of China(863Program),No.2008AA101005
文摘This study was designed to verify the stem cell properties of sheep amniotic epithelial cells and their capacity for neural differentiation. Immunofluorescence microscopy and reverse transcription-PCR revealed that the sheep amniotic epithelial cells were positive for the embryonic stem cell marker proteins SSEA-1, SSEA-3, SSEA-4, TRA-1-60 and TRA-1-81, and the totipotency-associated genes Oct-4, Sox-2 and Rex-1, but negative for Nanog. Amniotic epithelial cells expressed β-Ⅲ-tubulin, glial fibrillary acidic protein, nestin and microtubule-associated protein-2 at 28 days after induction with serum-free neurobasal-A medium containing B-27. Thus, sheep amniotic epithelial cells could differentiate into neurons expressing β-Ⅲ-tubulin and microtubule-associated protein-2, and glial-like cells expressing glial fibrillary acidic protein, under specific conditions.
基金supported by the Fostering Foundation for the Excellent Ph D.Dissertation of Gansu Agricultural University(2013002)the National High Technology Research and Development Program of China(2013AA102505)the Ministry of Science and Technology National Key R&D project(2016YFC0904600)
文摘In the present study, we used a proteomics approach based on a two-dimensional electrophoresis (2-DE) reference map to investigate protein expression in the ovarian tissues of pubertal Swiss-Webster mice subjected to carbon ion radiation (CIR). Among the identified proteins, ubiquitin carboxy-terminal hydrolase L1 (UCH-L1) is associated with the cell cycle[1] and that it influences proliferation in ovarian tissues. We analyzed the expression of UCH-L1 and the proliferation marker proliferation cell nuclear antigen (PCNA) following CIR using immunoblotting and immunofluorescence. The proteomics and biochemical results provide insight into the underlying mechanisms of CIR toxicity in ovarian tissues.
基金This work was supported by the research grant of the Kongju National University Industry-University Cooperation Foundation in 2024.
文摘Industrial wireless mesh networks(WMNs)have been widely deployed in various industrial sectors,providing services such as manufacturing process monitoring,equipment control,and sensor data collection.A notable characteristic of industrial WMNs is their distinct traffic pattern,where the majority of traffic flows originate from mesh nodes and are directed towards mesh gateways.In this context,this paper adopts and revisits a routing algorithm known as ALFA(autonomous load-balancing field-based anycast routing),tailored specifically for anycast(one-to-one-of-many)networking in WMNs,where traffic flows can be served through any one of multiple gateways.In essence,the scheme is a hybrid-type routing strategy that leverages the advantages of both back-pressure routing and geographic routing.Notably,its novelty lies in being developed by drawing inspiration from another field,specifically from the movement of charges in an electrostatic potential field.Expanding on the previous work,this paper explores further in-depth discussions that were not previously described,including a detailed description of the analogy between an electrostatic system and a WMN system based on precise mapping perspectives derived from intensive analysis,as well as discussions on anycast,numerical methods employed in devising the ALFA scheme,its characteristics,and complexity.It is worth noting that this paper addresses these previously unexplored aspects,representing significant contributions compared to previous works.As a completely new exploration,a new scheduling strategy is proposed that is compatible with the routing approach by utilizing the potential-based metric not only in routing but also in scheduling.This assigns higher medium access priority to links with a larger potential difference.Extensive simulation results demonstrate the superior performance of the proposed potential-based joint routing and scheduling scheme across various aspects within industrial WMN scenarios.
基金financially supported by the Strategic Priority Research Program of the Chinese Academy of Sciences (No.XDA16020405)National Key Research and Development Program (2020YFA0112603)the National Natural Science Foundation of China (Nos. 21902160 and 21821005)
文摘Mesenchymal stem cells(MSCs)have attracted great attention as a source of cells for regenerative medicine owing to their self-renewal ability and multiple differentiation potential.However,the serious shortage of MSCs and the difficulty of maintaining the differentiation potential during culture in vitro limit their application.Microcarrier culture technology is an effective method to realize large-scale culture of MSCs,whereas the effect of microcarriers properties on MSCs proliferation and differentiation potential maintenance should be investigated.In this study,Konjac glucomannan(KGM)microcarriers with a wide range of rigidity from 0.2 MPa to 10 MPa were prepared using water-in-oil emulsion polymerization.It was found that the microcarriers rigidity had great influence on MSCs by regulating cell spreading rate.Rapid proliferation and good differentiation potential maintenance of MSCs were achieved on the intermediate rigidity about 2.51±0.65 MPa with the faster spreading rate of cells.As a result,MSCs on 2.51±0.65 MPa KGM microcarriers proliferated about 27 times,which was 1.7 times as much as MSCs cultured on the commercial microcarriers Cytodex-1.The differentiation potential was also improved about 3.2 times compared to Cytodex-1.Therefore,it is indispensable to regulate microcarriers rigidity,especially for application of MSCs.
基金supported by the A*STAR under its"RIE2025 IAF-PP Advanced ROS2-native Platform Technologies for Cross sectorial Robotics Adoption(M21K1a0104)"programme.
文摘The motion planning problem for multi-agent systems becomes particularly challenging when humans or human-controlled robots are present in a mixed environment.To address this challenge,this paper presents an interaction-aware motion planning approach based on game theory in a receding-horizon manner Leveraging the framework provided by dynamic potential games for handling the interactions among agents,this approach formulates the multi-agent motion planning problem as a differential potential game,highlighting the effectiveness of constrained potential games in facilitating interactive motion planning among agents.Furthermore,online learning techniques are incorporated to dynamically learn the unknown preferences and models of humans or human-controlled robots through the analysis of observed data.To evaluate the effectiveness of the proposed approach,numerical simulations are conducted,demonstrating its capability to generate interactive trajectories for all agents,including humans and human-controlled agents,operating within the mixed environment.The simulation results illustrate the effectiveness of the proposed approach in handling the complexities of multi-agent motion planning in real-world scenarios.