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Vacuoles of Candida yeast as a specialized niche for Helicobacter pylori 被引量:4
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作者 Farideh Siavoshi Parastoo Saniee 《World Journal of Gastroenterology》 SCIE CAS 2014年第18期5263-5273,共11页
Helicobacter pylori(H.pylori)are resistant to hostile gastric environments and antibiotic therapy,reflecting the possibility that they are protected by an ecological niche,such as inside the vacuoles of human epitheli... Helicobacter pylori(H.pylori)are resistant to hostile gastric environments and antibiotic therapy,reflecting the possibility that they are protected by an ecological niche,such as inside the vacuoles of human epithelial and immune cells.Candida yeast may also provide such an alternative niche,as fluorescently labeled H.pylori were observed as fast-moving and viable bacterium-like bodies inside the vacuoles of gastric,oral,vaginal and foodborne Candida yeasts.In addition,H.pylori-specific genes and proteins were detected in samples extracted from these yeasts.The H.pylori present within these yeasts produce peroxiredoxin and thiol peroxidase,providing the ability to detoxify oxygen metabolites formed in immune cells.Furthermore,these bacteria produce urease and VacA,two virulence determinants of H.pylori that influence phago-lysosome fusion and bacterial survival in macrophages.Microscopic observations of H.pylori cells in new generations of yeasts along with amplification of H.pylori-specific genes from consecutive generations indicate that new yeasts can inherit the intracellular H.pylori as part of their vacuolar content.Accordingly,it is proposed that yeast vacuoles serve as a sophisticated niche that protects H.pylori against the environmental stresses and provides essential nutrients,including ergosterol,for its growth and multiplication.This intracellular establishment inside the yeast vacuole likely occurred long ago,leading to the adaptation of H.pylori to persist in phagocytic cells.The presence of these bacteria within yeasts,including foodborne yeasts,along with the vertical transmission of yeasts from mother to neonate,provide explanations for the persistence and propagation of H.pylori in the human population.This Topic Highlight reviews and discusses recent evidence regarding the evolutionary adaptation of H.pylori to thrive in host cell vacuoles. 展开更多
关键词 HELICOBACTER PYLORI Intracellular CANDIDA vacuole
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Vacuole import and degradation pathway: Insights into a specialized autophagy pathway
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作者 Abbas A Alibhoy Hui-Ling Chiang 《World Journal of Biological Chemistry》 CAS 2011年第11期239-245,共7页
Glucose deprivation induces the synthesis of pivotagluconeogenic enzymes such as fructose-1,6-bisphos-phatase, malate dehydrogenase, phosphoenolpyruvatecarboxykinase and isocitrate lyase in Saccharomycescerevisiae. Ho... Glucose deprivation induces the synthesis of pivotagluconeogenic enzymes such as fructose-1,6-bisphos-phatase, malate dehydrogenase, phosphoenolpyruvatecarboxykinase and isocitrate lyase in Saccharomycescerevisiae. However, following glucose replenishment,these gluconeogenic enzymes are inactivated and de-graded. Studies have characterized the mechanismsby which these enzymes are inactivated in response toglucose. The site of degradation of these proteins hasalso been ascertained to be dependent on the dura-tion of starvation. Glucose replenishment of short-termstarved cells results in these proteins being degradedin the proteasome. In contrast, addition of glucose tocells starved for a prolonged period results in theseproteins being degraded in the vacuole. In the vacuoledependent pathway, these proteins are sequestered inspecialized vesicles termed vacuole import and degra-dation (Vid). These vesicles converge with the endo-cytic pathway and deliver their cargo to the vacuolefor degradation. Recent studies have identified thatinternalization, as mediated by actin polymerization, isessential for delivery of cargo proteins to the vacuolefor degradation. In addition, components of the targetof rapamycin complex 1 interact with cargo proteins during glucose starvation. Furthermore, Tor1p dissoci-ates from cargo proteins following glucose replenish-ment. Future studies will be needed to elaborate on the importance of internalization at the plasma membrane and the subsequent import of cargo proteins into Vid vesicles in the vacuole dependent degradation pathway. 展开更多
关键词 vacuole IMPORT and degradation Fructose-1 6-bisphosphatase vacuole PROTEASOME AUTOPHAGY Target of rapamycin complex 1 Actin polymerization ENDOCYTOSIS
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Influence of vacuoles with gas–liquid inclusions on the thermobaric destruction conditions of natural quartz under dynamic heating in an RF-TICP torch system
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作者 苗龙 聂明卿 +7 位作者 Yuri Mihailovich GRISHIN 王晓宇 朱政羲 宋家辉 梁福文 何梓豪 田丰 王宁飞 《Plasma Science and Technology》 SCIE EI CAS CSCD 2023年第4期138-147,共10页
In the present work,the turbulent mixing process of a polydisperse quartz particle flow with a plasma stream generated by a radio-frequency(RF)inductively coupled plasma torch was numerically studied.The thermobaric s... In the present work,the turbulent mixing process of a polydisperse quartz particle flow with a plasma stream generated by a radio-frequency(RF)inductively coupled plasma torch was numerically studied.The thermobaric stress in the quartz particles under dynamic heating in a heterogeneous plasma flow was determined by a two-stage approximation approach.The effect of the presence of vacuoles in natural quartz on the particle thermobaric destruction conditions was studied.It was found that the equivalent thermal and baric stresses in quartz particles may significantly increase in the presence of vacuoles within a small gas volume fraction.The influence of the regime and energetic working conditions of an RF inductively coupled plasma torch system on the particle thermobaric destruction conditions was examined,and a recommendation was given to promote the degree of thermobaric destruction of quartz particles,which is of substantial importance for improving the overall enrichment efficiency of quartz concentrates. 展开更多
关键词 high purity quartz dynamic heating vacuole with gas–liquid inclusions thermobaric destruction radio-frequency inductively coupled plasma torch
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幽门螺杆菌空泡细胞毒素(Vacuolating cytotoxin,VacA)研究进展
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作者 赵雩卿 钱家鸣 《胃肠病学和肝病学杂志》 CAS 2004年第3期322-325,共4页
1988年Leunk等发现幽门螺杆菌(Helicobacter pylori,H.pylori)肉汤培养基的上清液含有一种使多种体外培养的真核细胞系发生空泡样变性的毒素,1992年这种毒素得以纯化并被命名为空泡细胞毒素(Vacuo-1ating cytotoxin,VacA).1994年完成了... 1988年Leunk等发现幽门螺杆菌(Helicobacter pylori,H.pylori)肉汤培养基的上清液含有一种使多种体外培养的真核细胞系发生空泡样变性的毒素,1992年这种毒素得以纯化并被命名为空泡细胞毒素(Vacuo-1ating cytotoxin,VacA).1994年完成了编码VacA的基因vacA的克隆和序列分析,由此VacA作为H.pylori致病的主要毒力因子引起了研究者的广泛兴趣和关注,本文将综述近十年有关VacA的研究进展. 展开更多
关键词 幽门螺杆菌 空泡细胞毒素 真核细胞 氨基酸 菌株 空泡毒素
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The Role of Vacuolar Processing Enzyme 被引量:1
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作者 王墨洋 韩宝达 +1 位作者 赵翔 李立新 《Agricultural Science & Technology》 CAS 2012年第5期945-951,共7页
The classification, characters and maturation methods of VPEs were sum- marized, and its regulation function to vacuolar was also analyzed. Furthermore, effects of the enzyme in vacuolar-mediated plant defense mechani... The classification, characters and maturation methods of VPEs were sum- marized, and its regulation function to vacuolar was also analyzed. Furthermore, effects of the enzyme in vacuolar-mediated plant defense mechanism were discussed to point out that VPEs were divided into 3 subfamilies via autocatalytic mature including seed-type VPE, vegetative-type VPE and new-type VPE. Especially, seed- type VPE mediated the process of storage protein, while vegetative-type VPE and new-type VPE regulated and controlled programmed death of plant cells. 展开更多
关键词 vacuole VPE Process and maturation of proteins Programmed cell death
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Effect of NaCI and Helicobacter pylori vacuolating cytotoxin on cytokine expression and viability 被引量:2
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作者 Juan Sun Kazuo Aoki +3 位作者 Jin-Xu Zheng Bing-Zhong Su Xiao-Hui Ouyang Junichi Misumi 《World Journal of Gastroenterology》 SCIE CAS CSCD 2006年第14期2174-2180,共7页
AIM; To determine whether Helicobacter pylori (H pylon) vacuolating cytotoxin (VacA) regulates release of proinflammatory cytokines (IL-1β, IL-8, TNF-α, and IL-6) or alters gastric epithelial cell viability an... AIM; To determine whether Helicobacter pylori (H pylon) vacuolating cytotoxin (VacA) regulates release of proinflammatory cytokines (IL-1β, IL-8, TNF-α, and IL-6) or alters gastric epithelial cell viability and to determine whether NaCl affects these VacA-induced changes. METHODS: Vacuolating activity was determined by measuring the uptake of neutral red into vacuoles of VacA-treated human gastric epithelial (AGS) cells. AGS cell viability was assessed by direct cell counting. Specific enzyme-linked immunosorbent assays (ELISA) and reverse transcdptase-polymerase chain reaction(RT-PCR) were performed to examine the effects of H pylori VacA and NaCl on cell pro-inflammatory cytokine production in AGS cells. Immunohistochemical staining of gastric tissue from Mongolian gerbils was used to confirm VacAinduced pro-inflammatory cytokine production and the effects of NaCl on this VacA-induced response. RESULTS: Addition of VacA alone reduced AGS cell viability (P〈 0.05), and this reduction was enhanced by high doses of NaCl (P〈0.05). VacA alone induced expression of TNF-α, IL-8 and IL-1β, while NaCl alone induced expression of TNF-α and IL-1β. Changes in mRNA levels in the presence of both VacA and NaCl were more complicated. For the case of TNF-α, expression was dosedependent on NaCl. IL-6 mRNA was not detected. However, low levels of IL-6 were detected by EUSA. Positive immunohistochemical staining of IL-1, IL-6, and TNF-α was found in gastric tissue of H pylori-infected gerbils fed with either a normal diet or a high salt diet. However, the staining of these three cytoldnes was sb'onger in H pylori-infected animals fed with a 5g/kg NaCl diet. CONCLUSION: VacA decreases the viability of AGS cells, and this effect can be enhanced by NaCl. NaCl also affects the production of pro-inflammatory cytokines in- duced by VacA, suggesting that NaCl plays an important role in Hpylori-induced gastric epithelial cell cytotoxicity. 展开更多
关键词 Helicobacter pylori vacuolating cytotoxin CYTOKINE GERBIL AGS cell
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Effect of the Vacuolation of Helicobacter Pylori
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作者 施理 候晓华 +1 位作者 易粹琼 张锦坤 《Journal of Huazhong University of Science and Technology(Medical Sciences)》 SCIE CAS 2001年第2期97-99,共3页
Cytotoxic test in vitro combined with cytochemical stain, fluorescent stain, transmission electronmicrograph was used to study the vacuolated effect by helicobacter pylori (H.pylori) (Toxin+) and its pathological ... Cytotoxic test in vitro combined with cytochemical stain, fluorescent stain, transmission electronmicrograph was used to study the vacuolated effect by helicobacter pylori (H.pylori) (Toxin+) and its pathological mechanism. 78..26 % patients with peptic ulcer associated with H.pylori was infected with H.pylori (Toxin+), while 42.86 % patients with gastritis was infected with H.pylori (Toxin+). It was positive in vacuole with acridine orange and acid phosphatase stain. Transmission electronmicrograph of vacuole revealed the presence of abounding membrane. There was a closed relationship between infection with H.pylori (Toxin+) and peptic ulcer disease. The vacuole induced by H.pylori (Toxin+) was autophagosome, which was pathological phenomenon induced by toxin. 展开更多
关键词 helicobacter pylori vacuolATION AUTOPHAGOSOME
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Cryopreservation enhances vacuolization in human spermatozoa
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作者 Nahid Yari Sahabeh Etebary +1 位作者 Mohammad Ali Khalili Ali Reza Talebi 《Asian pacific Journal of Reproduction》 2017年第6期247-251,共5页
Objective:To evaluate the impact of freezing–thawing on the human sperm head vacuoles and the potential value of motile sperm organelle morphology examination for selection of frozen-thaw spermatozoa.Methods: In 30 s... Objective:To evaluate the impact of freezing–thawing on the human sperm head vacuoles and the potential value of motile sperm organelle morphology examination for selection of frozen-thaw spermatozoa.Methods: In 30 sperm samples from infertile men, analysis for conventional sperm parameters (motility, vitality, and normal morphology) and a morphological analysis at high magnification for vacuoles examination were done before cooling and after warming. For description of sperm head vacuoles, two hundred spermatozoa were examined and were classified into three groups according to presence and vacuole areas including no vacuole group (free of any vacuole), small vacuole group (occupy not more than 4% of the nuclear area), and large vacuole group (occupy more than 4% of the normal nuclear area).Results:Significant reduction of progressive motility and vitality was observed following cryopreservation (P<0.001). Also, normal morphology decreased significantly after cryopreservation (P<0.05). Spermatozoa with a vacuole-free head had a significant reduction in cryopreservation group (P=0.013). The percentage of spermatozoa with small vacuoles increased slightly, but not significantly after cryopreservation (P=0.296).Conclusions:Motile sperm organelle morphology examination is a powerful research tool for investigating spermatozoa abnormalities such as vacuoles that are increased post cryopreservation. 展开更多
关键词 FREEZING MSOME SPERMATOZOA Thawing vacuoleS
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凹坑曲率对近壁单空泡溃灭动力学特性的影响
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作者 韩伟 郝英剑 +2 位作者 李仁年 任嘉乐 黄心愿 《浙江大学学报(工学版)》 EI CAS CSCD 北大核心 2024年第10期2111-2118,共8页
空蚀导致流体机械壁面出现凹坑,影响流体机械壁面周围的流场,使附近的空泡溃灭过程复杂.基于欧拉方法模拟凹坑壁面附近空泡溃灭过程,分析球形空泡在不同壁面曲率下的动力学特性.结果表明,空泡溃灭时间与凹坑相对曲率呈指数函数关系,与... 空蚀导致流体机械壁面出现凹坑,影响流体机械壁面周围的流场,使附近的空泡溃灭过程复杂.基于欧拉方法模拟凹坑壁面附近空泡溃灭过程,分析球形空泡在不同壁面曲率下的动力学特性.结果表明,空泡溃灭时间与凹坑相对曲率呈指数函数关系,与空泡近壁系数呈线性关系且单调递减;空泡射流马赫数与凹坑相对曲率呈线性关系且单调递减,与空泡近壁系数呈线性关系且单调递增;冲击载荷与空泡溃灭时间呈二次函数关系且单调递减,与凹坑相对曲率和空泡近壁系数呈线性关系且单调递减. 展开更多
关键词 空泡动力学 凹坑相对曲率 射流速度 冲击载荷 空泡溃灭
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内质网应激诱导细胞类凋亡的机制研究进展 被引量:2
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作者 吴瑜婷 郑雨彤 +1 位作者 石惠萍 胡琼莹 《台州学院学报》 2024年第3期63-71,共9页
类凋亡(paraptosis)是一种新的程序性细胞死亡方式,以内质网和线粒体肿胀、细胞质空泡化为典型特征。有研究表明:内质网应激(endoplasmicreticulumstress,ERS)不仅可以调控类凋亡,还有可能与多种类凋亡信号通路发生作用。文章对内质网... 类凋亡(paraptosis)是一种新的程序性细胞死亡方式,以内质网和线粒体肿胀、细胞质空泡化为典型特征。有研究表明:内质网应激(endoplasmicreticulumstress,ERS)不仅可以调控类凋亡,还有可能与多种类凋亡信号通路发生作用。文章对内质网应激与类凋亡之间的关系及调控机制进行了综述,并探讨了目前类凋亡研究尚未解决的问题,力求从中探寻内质网应激在类凋亡中的具体作用机制,以期为类凋亡相关的药物作用靶点的发现及药物研发提供理论依据和参考。 展开更多
关键词 细胞程序性死亡 类凋亡 内质网应激 细胞质空泡化
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马度米星铵对成肌细胞转录组和超微结构的影响
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作者 陈新 刘畅 张雨梅 《中国兽药杂志》 2024年第6期40-46,共7页
探究马度米星铵对成肌细胞基因表达和超微结构的影响。马度米星铵处理C2C12细胞后,于细胞实时分析仪中记录细胞生长情况;利用Illumina HiSeq平台进行转录组测序,筛选差异表达基因并进行基因本体富集分析;显微镜下观察细胞空泡情况并拍照... 探究马度米星铵对成肌细胞基因表达和超微结构的影响。马度米星铵处理C2C12细胞后,于细胞实时分析仪中记录细胞生长情况;利用Illumina HiSeq平台进行转录组测序,筛选差异表达基因并进行基因本体富集分析;显微镜下观察细胞空泡情况并拍照;透射电镜下观察拍照细胞超微结构。结果表明,马度米星铵抑制C2C12细胞生长,呈浓度和时间相关性;1μM马度米星铵处理细胞8 h后,差异表达基因共2450个,其中下调基因为1411个,上调基因1039个;基因本体分析发现,差异表达基因显著富集到空泡、DNA修复、转移酶活性正调控等;细胞内空泡显著增加;1μM马度米星铵处理细胞24 h后,细胞器明显肿胀、线粒体嵴结构髓样变、粗面内质网表面附着核糖体脱颗粒。由此可知,马度米星铵体外可诱导C2C12细胞空泡化,破坏细胞器结构,抑制细胞生长,从而导致其骨骼肌成肌细胞毒性作用,干预细胞空泡化是预防和治疗马度米星铵中毒的重要途径。 展开更多
关键词 马度米星铵 转录组 空泡 超微结构 成肌细胞
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从自噬和副凋亡途径初探桔梗皂苷-D抗乳腺癌作用与机制
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作者 刘玲琳 毛娟娟 +1 位作者 岑梦燕 杨扬 《浙江中西医结合杂志》 2024年第8期714-719,共6页
目的从自噬和副凋亡途径探讨桔梗皂苷-D(PD)抗乳腺癌的作用和潜在机制。方法结晶紫法检测不同浓度PD(10、20、40μmol/L)对三种乳腺癌细胞株4T1、MDA-MB-231、MCF-7细胞存活率的影响;MDC荧光标记法与LC3B荧光染色激光共聚焦显微镜观察... 目的从自噬和副凋亡途径探讨桔梗皂苷-D(PD)抗乳腺癌的作用和潜在机制。方法结晶紫法检测不同浓度PD(10、20、40μmol/L)对三种乳腺癌细胞株4T1、MDA-MB-231、MCF-7细胞存活率的影响;MDC荧光标记法与LC3B荧光染色激光共聚焦显微镜观察适宜浓度PD作用乳腺癌细胞后自噬发生的情况;PD联合自噬抑制剂氯喹作用三种乳腺癌细胞株,明确PD诱导自噬在细胞死亡中的作用;光镜与电镜下观察适宜浓度PD作用后乳腺癌细胞形态学的变化。结果与PD 0μmol/L组比较、PD20、40μmol/L组4T1[(63.10±2.15)%、(32.28±1.19)比(99.75±4.20),P<0.01]、MDA-MB-231[(63.10±2.15)%、(52.87±3.46)%和(82.04±5.27)%,P均<0.01]、MCF-7[(32.38±1.20)%、(3.25±0.627)%、(59.30±5.72)%,P均<0.01]细胞存活率均明显降低。MDC法与LC3B荧光染色法发现经PD处理的三种乳腺癌细胞后发生自噬现象。与PD组比较,PD联合氯喹组可进一步降低4T1、MDA-MB-231和MCF-7三种乳腺癌细胞株的存活率[4T1:(23.31±1.07)%比(40.35±2.94)%,P<0.01;MDA-MB-231:(31.40±2.73)%比(56.69±4.02)%,P<0.01;MCF-7:(52.72±4.50)%比(92.24±7.85)%,P<0.001]。光镜与电镜观察经PD处理的三种细胞质内出现大量空泡结构,并伴有线粒体肿胀,类似副凋亡的形态学改变。结论PD具有明确的抑制乳腺癌细胞株4T1、MDA-MB-231和MCF-7存活率的效应,该效应伴随自噬现象的发生,但自噬抑制剂的加入提示PD诱导的细胞自噬是对自身的保护作用。经PD处理的三种乳腺癌细胞出现类似副凋亡的形态学改变,初步推测PD对乳腺癌细胞的抑制作用可能与副凋亡相关。 展开更多
关键词 乳腺癌 桔梗皂苷-D 自噬 副凋亡 胞质空泡化
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海南省39株幽门螺杆菌的耐药性及毒力基因特征
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作者 卓书伟 林美幸 +4 位作者 吴翔 潘静琳 周海清 陈海 陈峥宏 《贵州医科大学学报》 CAS 2024年第4期479-488,共10页
目的了解海南省幽门螺杆菌(Hp)耐药性及其毒力基因vacA、cagA和iceA基因型特征。方法对146例上消化道症状疾病患者进行胃镜检查,取胃黏膜组织进行Hp的分离和培养,对Hp进行药敏检测;通过聚合酶链式反应(PCR)对毒力基因cagA、vacA和iceA... 目的了解海南省幽门螺杆菌(Hp)耐药性及其毒力基因vacA、cagA和iceA基因型特征。方法对146例上消化道症状疾病患者进行胃镜检查,取胃黏膜组织进行Hp的分离和培养,对Hp进行药敏检测;通过聚合酶链式反应(PCR)对毒力基因cagA、vacA和iceA进行检测和分型,cagA测序进行聚类分析、并构建系统进化树;使用Fisher确切概率法分析各型毒力基因和患者疾病发生的相关性。结果共培养出39株Hp,对阿莫西林和克拉霉素的耐药率分别为2.6%和48.7%;39株均为vacA^(+)菌株,iceA 1型26株、2型13株;33株为cagA^(+)菌株,其中5株为西方型(EPIYA-ABC型),28株为东亚型(EPIYA-ABD型);cagA^(+)和vacA^(+)菌株与胃和十二指肠疾病有显著的相关性(P<0.05),iceA则无显著性差异(P>0.05)。结论海南省胃和十二指肠Hp患者中Hp毒力基因多样化,vacA s1am2/cagA^(+)基因型在消化性溃疡患者中占优势,不同毒力基因型对胃和十二指肠疾病的发生具有明显的差别,阿莫西林可作为该地区根除Hp首选抗生素。 展开更多
关键词 幽门螺杆菌 耐药性 基因多样性 消化性溃疡 CAGA VACA ICEA
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The metal tolerance protein OsMTP11 facilitates cadmium sequestration in the vacuoles of leaf vascular cells for restricting its translocation into rice grains
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作者 Peng Liu Liang Sun +15 位作者 Yu Zhang Yongjun Tan Yuxing Zhu Can Peng Jiurong Wang Huili Yan Donghai Mao Guohua Liang Gang Liang Xiaoxiang Li Yuntao Liang Feng Wang Zhenyan He Wenbang Tang Daoyou Huang Caiyan Chen 《Molecular Plant》 SCIE CSCD 2024年第11期1733-1752,共20页
Rice(Oryza sativa)provides>20%of the consumed calories in the human diet.However,rice is also a leading source of dietary cadmium(Cd)that seriously threatens human health.Deciphering the genetic network that underl... Rice(Oryza sativa)provides>20%of the consumed calories in the human diet.However,rice is also a leading source of dietary cadmium(Cd)that seriously threatens human health.Deciphering the genetic network that underlies the grain-Cd accumulation will benefit the development of low-Cd rice and mitigate the effects of Cd accumulation in the rice grain.In this study,we identified a QTL gene,OsCS1,which is allelic to OsMTP11 and encodes a protein sequestering Cd in the leaf during vegetative growth and preventing Cd from being translocated to the grain after heading in rice.OsCS1 is predominantly expressed in leaf vascular parenchyma cells,where it binds to a vacuole-sorting receptor protein OsVSR2 and is translocated intracellularly from the trans-Golgi network to pre-vacuolar compartments and then to the vacuole.In this trafficking process,OsCS1 actively transports Cd into the endomembrane system and sequesters it in the vacuoles.There are natural variations in the promoter of OsCS1 between the indica and japonica rice subspecies.Duplication of a G-box-like motif in the promoter region of the superior allele of OsCS1 from indica rice enhances the binding of the transcription factor OsIRO2 to the OsCS1 promoter,thereby promoting OsCS1 expression.Introgression of this allele into commercial rice varieties could significantly lower grain-Cd levels compared to the inferior allele present in japonica rice.Collectively,our findings offer new insights into the genetic control of leaf-to-grain Cd translocation and provide a novel gene and its superior allele for the genetic improvement of low-Cd variety in rice. 展开更多
关键词 rice OsCS1/OsMTP11 grain Cd TGN–PVC–vacuole transport allelic variation
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温度对鸡胚成纤维细胞UMNSAH/DF-1的影响
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作者 张雨晴 夏晴 +1 位作者 喻峰 吴军 《科学技术创新》 2024年第9期38-41,共4页
本文通过在不同温度条件下进行鸡胚成纤维细胞UMNSAH/DF-1细胞培养实验,同时模拟活细胞株常温运输环境寻找改善UMNSAH/DF-1细胞生长状态及胞体“空泡化”现象的方法。实验结果表明:相较于37℃,39℃的培养温度更有利于UMNSAH/DF-1细胞生... 本文通过在不同温度条件下进行鸡胚成纤维细胞UMNSAH/DF-1细胞培养实验,同时模拟活细胞株常温运输环境寻找改善UMNSAH/DF-1细胞生长状态及胞体“空泡化”现象的方法。实验结果表明:相较于37℃,39℃的培养温度更有利于UMNSAH/DF-1细胞生长。室温运输时UMNSAH/DF-1细胞“空泡化”程度显著。但是正常传代后,其胞体“空泡化”现象又会显著减少。本文提示:若UMNSAH/DF-1细胞胞体“空泡”数量较多时,可通过优化该细胞最佳生长温度同时增加传代次数进行改善,一般多次传代后UMNSAH/DF-1细胞就会恢复至其正常状态。 展开更多
关键词 温度 鸡胚成纤维细胞 空泡化
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急性胰腺炎发病机制及并发内脏静脉血栓研究进展
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作者 莫闲 杨闯 《陕西医学杂志》 CAS 2024年第2期282-285,共4页
急性胰腺炎(AP)具有进展快、病死率高等特点,其发病因素较为复杂,是临床常见急腹症,临床上依据其器官功能衰竭和并发症发生情况可分为轻度、中度和重度AP。目前有关AP发生机制尚未完全阐明,主要存在胰酶自噬、胰泡细胞凋亡、炎症损伤、... 急性胰腺炎(AP)具有进展快、病死率高等特点,其发病因素较为复杂,是临床常见急腹症,临床上依据其器官功能衰竭和并发症发生情况可分为轻度、中度和重度AP。目前有关AP发生机制尚未完全阐明,主要存在胰酶自噬、胰泡细胞凋亡、炎症损伤、钙离子超载等理论。内脏静脉血栓(SVT)是AP常见并发症,其中以脾静脉、门静脉血栓较为常见,SVT可导致AP患者出现胃底静脉大出血、肠缺血坏死等,增加AP患者死亡风险。早期预防SVT发生风险,是改善AP预后的有效手段,临床上常通过药物、液体复苏、营养支持、手术等方法来预防SVT发生。现就其发病机制以及合并SVT进行综述。 展开更多
关键词 急性胰腺炎 内脏静脉血栓 钙离子 胰泡细胞 炎症 研究现状
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鱼腥藻PCC7120细胞液泡的初步研究 被引量:10
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作者 郭厚良 金传荫 +3 位作者 浦秋文 宋文贞 周云珍 杨清平 《水生生物学报》 CAS CSCD 北大核心 1999年第4期363-367,共5页
从保存3个月以上的老化培养物中直接检查到游离液泡。液泡为标准圆球状,完全透明,大小相差极为悬殊,多数大型液泡吞噬了数个衰老藻细胞。采用低渗酶解,渗透冲击,低渗酶解和渗透冲击相结合,从培养3个月以上,2个月,1个月,1... 从保存3个月以上的老化培养物中直接检查到游离液泡。液泡为标准圆球状,完全透明,大小相差极为悬殊,多数大型液泡吞噬了数个衰老藻细胞。采用低渗酶解,渗透冲击,低渗酶解和渗透冲击相结合,从培养3个月以上,2个月,1个月,18d,10d及3d的藻丝细胞都分离到液泡。液泡略大于细胞,泡内无吞噬物。培养3d的藻丝有15%的细胞分离到液泡。其他多种蓝藻也分离到同样的液泡。 展开更多
关键词 鱼腥藻PCC7120 液泡 低渗酶解 蓝藻 细胞
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植物液泡中硝酸盐行为的研究概况 被引量:52
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作者 沈其荣 汤利 徐阳春 《土壤学报》 CAS CSCD 北大核心 2003年第3期465-470,共6页
化学氮肥利用率低和损失严重而造成的环境污染问题是农业生态系统中氮素管理首当其冲要研究和解决的问题 ,这方面的研究工作在国内外报道浩如烟海 ,但突破性进展不多。另一方面 ,植物体内的硝酸盐含量高又严重影响农产品有关的品质性状 ... 化学氮肥利用率低和损失严重而造成的环境污染问题是农业生态系统中氮素管理首当其冲要研究和解决的问题 ,这方面的研究工作在国内外报道浩如烟海 ,但突破性进展不多。另一方面 ,植物体内的硝酸盐含量高又严重影响农产品有关的品质性状 ,特别是我国加入WTO后蔬菜和果实中高含量的硝酸盐是影响这些产品出口的主要限制因子之一。因此 ,从植物体本身着手研究植物氮素高效利用的机理与途径 ,这是近几年来植物氮素营养研究的热点之一。植物液泡占据了成熟植物细胞体积的 90 %左右 ,而液泡和细胞质中硝酸盐的浓度通常分别在 30~ 5 0molm- 3和 3~ 5molm- 3,因此 ,如何调动植物液泡中的硝酸盐使之得到更高程度的再利用 ,这是提高植物氮素利用效率和降低植物体内硝酸盐含量的途径之一。本文综述了国内外有关液泡中硝酸盐行为的研究状况 ,在此基础上作者首次提出植物液泡中硝酸盐的内外流与植物氮素高效利用之间可能存在着密切的关系 ,旨在拓始这方面的工作能尽快开展 ,为植物氮素高效利用的分子生物学研究开辟新的研究领域。 展开更多
关键词 植物 液泡 硝酸盐 氮肥 利用率
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KCl诱导柱胞鱼腥藻形成液泡 被引量:13
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作者 郭厚良 黄开耀 +1 位作者 易平 金传荫 《水生生物学报》 CAS CSCD 北大核心 1998年第2期198-199,共2页
关键词 柱胞鱼腥藻 液泡 KCL
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巨泡式死亡:一种新的细胞死亡方式 被引量:6
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作者 蔡红兵 刘金坤 +1 位作者 范钦 李欣 《南方医科大学学报》 CAS CSCD 北大核心 2013年第12期1844-1847,共4页
细胞死亡是生命活动过程中一项重要的生理或病理现象。传统的细胞死亡方式主要包括凋亡、坏死和自噬等。新近研究发现了一种新的细胞死亡方式-methuosis,在这种细胞死亡过程中,由于过度刺激导致细胞内水泡吸收、积累、融合而逐步形成相... 细胞死亡是生命活动过程中一项重要的生理或病理现象。传统的细胞死亡方式主要包括凋亡、坏死和自噬等。新近研究发现了一种新的细胞死亡方式-methuosis,在这种细胞死亡过程中,由于过度刺激导致细胞内水泡吸收、积累、融合而逐步形成相对于细胞本身而言的大量巨大液泡,最终导致细胞代谢活动减少、细胞膜破裂、细胞死亡。本文对巨泡式死亡的命名来源、形态特征、可能的机制、与相关死亡方式的区别及其研究进展进行了阐述。 展开更多
关键词 细胞死亡 methuosis 巨泡式死亡 细胞质空泡化
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