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A New Approach for the Measurement of Digestible Carbohydrates in Different Food Samples with HPLC-RI
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作者 S. Devindra T. Longvah 《Journal of Agricultural Science and Technology(B)》 2011年第8期1216-1223,共8页
This paper gives an impetus in quantifying digestible carbohydrates, which are important components of cereals, legumes and vegetables. The HPLC method was applied for the first time in India to estimate the digestibl... This paper gives an impetus in quantifying digestible carbohydrates, which are important components of cereals, legumes and vegetables. The HPLC method was applied for the first time in India to estimate the digestible carbohydrates such as sugars and starches in different branded rice, legumes and vegetable samples which were procured from local market of twin cities of Hyderabad and Secunderabad in Andhra Pradesh State, India. In the present study, we estimated carbohydrates in vitro, mimicking the in vivo condition by incorporating enzymatic digestion. Among the rice varieties the analyzed total soluble sugars were in the range of 5.69% to 9.62%, vegetables 0.0% to 3.72% and legumes 0.04% to 0.98%. Soluble starches in rice samples ranged from 12.51% to 17.64%, in vegetables from 1.73% to 7.28% and in legumes from 23.76% to 38.71%. Insoluble starches in rice samples were observed to be bracketed in between 52.53% to 60.43%, where as in vegetables from 0.58% to 8.83% and in legumes from 16.13% to 29.22%. Concluding our observation, the total amount of starches and total sugars in rice fell in between 74.32% to 80.75%, in vegetables from 1.74% to 16.11%, and in legumes from 39.93% to 68.91%. 展开更多
关键词 STARCHES a-amylase PROTEASE AMYLOGLUCOSIDASE HPLC-RI method digestible carbohydrates.
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