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基于Digital Micrograph的Ti(C,N)基金属陶瓷材料定量金相分析 被引量:1
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作者 侯东芳 刘文俊 汪业 《三峡大学学报(自然科学版)》 CAS 2010年第2期72-74,共3页
Ti(C,N)基金属陶瓷显微组织及相界面较复杂,需要一种简便、精确的定量金相方法来辅助研究.采用功能强大的Gatan Digital Micrograph软件对不同纳米添加量Ti(C,N)基金属陶瓷显微组织进行背底消除、滤波、二值化及图像形态学处理,分析可... Ti(C,N)基金属陶瓷显微组织及相界面较复杂,需要一种简便、精确的定量金相方法来辅助研究.采用功能强大的Gatan Digital Micrograph软件对不同纳米添加量Ti(C,N)基金属陶瓷显微组织进行背底消除、滤波、二值化及图像形态学处理,分析可得到该金相图中的一些金相特征参数.对不同纳米添加量Ti(C,N)基金属陶瓷中硬质相进行了综合分析,结果表明,利用Gatan Digi-tal Micrograph软件实现Ti(C,N)基金属陶瓷显微组织的分析测量是完全可行的,能有效地提高工作效率及得到直观、准确的结果,也可定量地得到随纳米粉添加量的不断增加,硬质相颗粒的总体积不断减少,平均粒径也在不断减小. 展开更多
关键词 TI(c N)基金属陶瓷 Gatan digital Micrograph 图像处理 显微组织
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Study of time-domain digital pulse shaping algorithms for nuclear signals 被引量:37
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作者 ZHOU Jianbin ZHOU Wei +3 位作者 LEI Jiarong TUO Xianguo ZHU Xing LIU Yi 《Nuclear Science and Techniques》 SCIE CAS CSCD 2012年第3期150-155,共6页
With the development on high-speed integrated circuit,fast high resolution sampling ADC and digital signal processors are replacing analog shaping amplifier circuit.This paper firstly presents the numerical analysis a... With the development on high-speed integrated circuit,fast high resolution sampling ADC and digital signal processors are replacing analog shaping amplifier circuit.This paper firstly presents the numerical analysis and simulation on R-C shaping circuit model and C-R shaping circuit model.Mathematic models are established based on 1st order digital differential method and Kirchhoff Current Law in time domain,and a simulation and error evaluation experiment on an ideal digital signal are carried out with Excel VBA.A digital shaping test for a semiconductor X-ray detector in real time is also presented.Then a numerical analysis for Sallen-Key(S-K) low-pass filter circuit model is implemented based on the analysis of digital R-C and digital C-R shaping methods.By applying the 2nd order non-homogeneous differential equation,the authors implement a digital Gaussian filter model for a standard exponential-decaying signal and a nuclear pulse signal.Finally,computer simulations and experimental tests are carried out and the results show the possibility of the digital pulse processing algorithms. 展开更多
关键词 数字信号处理器 脉冲整形 算法 时域 基尔霍夫电流定律 计算机模拟 非齐次微分方程 电路模型
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Intelligent customization of digital carpet based on C2B 被引量:1
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作者 王波 《石化技术》 CAS 2018年第1期36-36,共1页
New requirements have been put forward by the age of "Internet+" to improve the abilities of reform,transformation and innovation for traditional carpet industry in combining the technology of the fully digi... New requirements have been put forward by the age of "Internet+" to improve the abilities of reform,transformation and innovation for traditional carpet industry in combining the technology of the fully digital fabric tufting and the system of networked intelligent customization that forms C2B mode of personalized customization of the digital carpet to promote the carpet industry to develop towards individuation,intelligence and high efficiency. Exploring the market background of C2B mode of intelligent customization of the digital carpet and developing the technology of the fully digital fabric tufting makes it possible to achieve personalized customization of the digital carpet. The industrial internet which is integrated by a lot of robots of the fully digital fabric tufting will change completely traditional carpet industry. 展开更多
关键词 digital cARPET INdiVIDUATION intelligent cUSTOMIZATION c2B industrial internet
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Digital arc welding power supply based on real-time operating system
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作者 张红兵 黄石生 周漪清 《China Welding》 EI CAS 2007年第3期1-5,共5页
A digital arc welding power supply was designed with the advanced reduced instruction set computer machine (ARM) and embedded real-time multi-task operating system micro C/OS- Ⅱ. The ARM, with its powerful calculat... A digital arc welding power supply was designed with the advanced reduced instruction set computer machine (ARM) and embedded real-time multi-task operating system micro C/OS- Ⅱ. The ARM, with its powerful calculating speed and complete peripheral equipments, is very suitable to work as the controller of the digital power supply. The micro C/OS- Ⅱ transplanted in ARM, helps to improve the respondent speed against various welding signals, as well as the reliability of the controlling software. The welding process consists of nine tasks. The tasks of great significance on reliability of the welder, for example, the A/D conversion of current and voltage, enjoy top priority. To avoid simultaneous-sharing on A/D converter and LCD module, two semaphores are introduced in to ensure the smooth performance of the welding power supply. Proven by experiments ,the ARM and the micro C/OS- Ⅱ can greatly improve both the respondent speed and the reliability of the digital welder. 展开更多
关键词 micro c/OS-Ⅱ real-time operating system digitIZATION arc welding power supply
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The Design of Digital Excitation Regulator
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作者 TIZong-li ZHAOYu-lin 《Journal of Northeast Agricultural University(English Edition)》 CAS 2001年第1期49-54,共6页
The digital excitation regulator is very popular in the small electric plant. This kind of regulator can im- prove the working condition of generator and its working efficiency. It is on the base of the 80C51 Micro-co... The digital excitation regulator is very popular in the small electric plant. This kind of regulator can im- prove the working condition of generator and its working efficiency. It is on the base of the 80C51 Micro-con- troller. The regulator can automatically finish some functions through the combination of software and hard- ware. Through the experiment we can know it is more intellectual than the traditional analog excitation regu- lator. 展开更多
关键词 digital excitation regulator 80c51 Micro-controller ANALOG
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Research on the Teaching System of Creative and Entrepreneurial Software Engineering(Digital Media Technology)Talents Training
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作者 Lifang Chen Yuan Liu +2 位作者 Fei Chen Jie Zhang Jing Lu 《计算机教育》 2018年第12期75-81,共7页
The cultivation of innovative and entrepreneurial capability is a quality requirement of the times for the training of college and university talents. However, the regular teaching system universally has such problems... The cultivation of innovative and entrepreneurial capability is a quality requirement of the times for the training of college and university talents. However, the regular teaching system universally has such problems as much more emphasis on theory than on practice, much more emphasis on inheritance than on innovation, and mismatching between talent training and market demand, so that a great number of graduates do not have enough innovative capacity, and it is very difficult for them to engage in related jobs of software engineering steadily and permanently. By investigating and analyzing similar majors of more than 20 schools at home and abroad, this article proposes a teaching system of talent training of the 3 C-cored major of "innovative and entrepreneurial" software engineering(digital media technology) based on regions, oriented to the industry and marked by school-enterprise linkage, and in connection with the characteristics of college and university education. 展开更多
关键词 3c INNOVATIVE and ENTREPRENEURIAL TEAcHING system digital MEdiA technology software engineering
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The Mathematical and Physical Theory of Rational Human Intelligence: Complete Empirical-Digital Properties;Full Electrochemical-Mechanical Model (Part I: Mathematical Foundations)
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作者 Leo Depuydt 《Advances in Pure Mathematics》 2013年第5期491-561,共71页
The design of this paper is to present the first installment of a complete and final theory of rational human intelligence. The theory is mathematical in the strictest possible sense. The mathematics involved is stric... The design of this paper is to present the first installment of a complete and final theory of rational human intelligence. The theory is mathematical in the strictest possible sense. The mathematics involved is strictly digital—not quantitative in the manner that what is usually thought of as mathematics is quantitative. It is anticipated at this time that the exclusively digital nature of rational human intelligence exhibits four flavors of digitality, apparently no more, and that each flavor will require a lengthy study in its own right. (For more information,please refer to the PDF.) 展开更多
关键词 Artificial INTELLIGENcE Boolean ALGEBRA Boole’s ALGEBRA Black Box Theories Brain Science cognition cognitive Science digital MATHEMATIcS Electricity and Magnetism J.-L. Lagrange and Partial differential Equations J. c. Maxwell’s Theory of Electromagnetism Neuroscience Non-Quantitative and Quantitative MATHEMATIcS Physics RATIONAL Human INTELLIGENcE cOMPLETE Theory of RATIONAL Thought and Language
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Design of digital control DC voltage source based on AT89C52 MCU
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作者 Wang wei 《International Journal of Technology Management》 2013年第1期107-108,共2页
the system to DC voltage source as the core, AT89C52 MCU as the main controller, the output voltage to set the DC power supply through the keyboard, with a step function voltage to reality, the actual output value. Th... the system to DC voltage source as the core, AT89C52 MCU as the main controller, the output voltage to set the DC power supply through the keyboard, with a step function voltage to reality, the actual output value. This design is divided into four modules: SCM control and display module, digital to analog (D/A) conversion module, a constant voltage source module, output module. MCU control module as the core, the input signal is converted to digital quantity output; constant current source module voltage D/A conversion to analog conversion into constant pressure through a constant voltage circuit. The system has good reliability, high precision. 展开更多
关键词 digital voltage source AT89c52 DAc0832 constant current source
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Digital Instrumentation and Control System for Unit 5 & 6 of YangJiang NPP
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作者 Yongbin Sun 《Journal of Energy and Power Engineering》 2014年第10期1777-1782,共6页
The article describes the digital instrumentation and control system for unit 5 & 6 of YangJiang NPP, involving the overall I & C (instrumentation and control) structure, the basic requirements and independent ver... The article describes the digital instrumentation and control system for unit 5 & 6 of YangJiang NPP, involving the overall I & C (instrumentation and control) structure, the basic requirements and independent verification and validation. Advanced I & C systems for YangJiang NPPs have to meet increasing demands for safety and availability. Additionally, the specific requirements coming from the nuclear qualification have to be fulfilled. 展开更多
关键词 digital I c safety class V V (verification and validation) overall I c structure McR (main control room) ScID(safety control and information display) DAS (diversity actuation system).
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嗜热链球菌c-di-AMP合成酶的结构预测
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作者 范艺周 骆思羽 +3 位作者 朱浩伟 薛珍 艾连中 熊智强 《工业微生物》 CAS 2024年第1期171-176,共6页
作为一种新兴第二信使分子,c-di-AMP具有全局调控胞内渗透压平衡、DNA损伤和生物膜形成等生理功能。本实验室在实验前期筛选出了一株高产胞外多糖的嗜热链球菌菌株S-3,但未对其进行c-di-AMP信号通路研究。本研究在嗜热链球菌S-3全基因... 作为一种新兴第二信使分子,c-di-AMP具有全局调控胞内渗透压平衡、DNA损伤和生物膜形成等生理功能。本实验室在实验前期筛选出了一株高产胞外多糖的嗜热链球菌菌株S-3,但未对其进行c-di-AMP信号通路研究。本研究在嗜热链球菌S-3全基因组中筛选出c-di-AMP合成酶(StDAC),对其二级结构进行预测,结果显示St DAC二级结构包含42.98%α-螺旋、15.32%β-折叠和38.30%无规则卷曲。利用SWISS-MODEL和Modeller对StDAC进行单模板和多模板同源建模,由SWISS-MODEL建立的模型通过了Ramachandran、Errat和Verify-3D评估,表明其结构较为合理;而Modeller建立的模型仅通过了Ramachandran评估,总体结构评分较低。本研究预测的嗜热链球菌StDAC三维模型为解析其结构和功能奠定了基础。 展开更多
关键词 嗜热链球菌 c-di-AMP 生物信息学 二级结构 同源建模
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Comparative Study of Radiological Changes in Hands and Feet in Patients Suffering from Early Rheumatoid Arthritis by Power Doppler Ultrasound and Direct Digital Radiography
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作者 Gargy Mondal Biswadip Ghosh +1 位作者 Manoranjan Ghosh Pampa Halder 《Open Journal of Rheumatology and Autoimmune Diseases》 2014年第1期39-42,共4页
Rheumatoid arthritis is a chronic multisystem disease of unknown cause. The characteristic feature of RA is persistent inflammatory synovitis. The natural history of disease is such that the early months of the diseas... Rheumatoid arthritis is a chronic multisystem disease of unknown cause. The characteristic feature of RA is persistent inflammatory synovitis. The natural history of disease is such that the early months of the disease are critical period during which reversible joint damage occurs. So early diagnosis of RA and appropriate drug application is the only way to save a patient from this crippling disease. In India, the cost of investigations is a significant factor for most of the patients. Ultrasonography or Power Doppler Ultra Sound (PDUS) has the advantage of being economic in spite of its sensitivity in assessing both inflammatory and destructive changes. The aim of the present study was to evaluate the diagnostic efficiency of PDUS in early rheumatoid arthritis. The study was performed with the patients attending Rheumatology Clinic. A total number of 106 patients of clinically suspected rheumatoid arthritis were studied as per selection criteria. Radiological examinations of hands were done by digital radiography and PDUS in a group of 53 patients, assessment of foot changes by PDUS and Digital Radiography were done in another similar group of 53 patients. Final diagnosis by ACR EULAR-2010 criteria is done for all the patients. The comparative study reveals that synovial vascularity as demonstrated by PDUS is much more effective in diagnosing early rheumatoid arthritis, both in hand and in feet than digital radiograph. PDUS of feet may yield earlier and better findings than hands, which is conventionally used in patients suffering from early rheumatoid arthritis. 展开更多
关键词 R.A.—Rheumatoid Arthritis PDUS—Power Doppler Ultrasound D.R.—digital Radiography AcR-EULAR—American college of RHEUMATOLOGY (AcR) and the European LEAGUE against RHEUMATISM (EULAR) RF—Rheumatoid Factor cRP—c-Reactive Protein MRI—Magnetic Resonance Imaging
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乳酸菌第二信使分子c-di-AMP研究进展
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作者 范艺周 李欢欢 +3 位作者 喻林兵 曹佳尧 艾连中 熊智强 《工业微生物》 CAS 2024年第2期79-84,共6页
微生物依赖信号识别和信号传递来感知并响应外界环境变化。c-di-AMP作为第二信使分子在信号传导过程中发挥着关键作用,其通过酶、转录调控因子和核糖开关等受体靶标来调控细胞生长、生物膜形成、K^(+)稳态、DNA完整性、细胞壁合成和脂... 微生物依赖信号识别和信号传递来感知并响应外界环境变化。c-di-AMP作为第二信使分子在信号传导过程中发挥着关键作用,其通过酶、转录调控因子和核糖开关等受体靶标来调控细胞生长、生物膜形成、K^(+)稳态、DNA完整性、细胞壁合成和脂肪酸合成等与生理功能相关的基因和蛋白的表达。尽管对于c-di-AMP代谢调控在枯草芽孢杆菌、单增李斯特氏菌和金黄色葡萄球菌等细菌中的研究较为深入,但对其在乳酸菌中的研究却相对较少。基于此,文章重点关注乳酸菌中cdi-AMP的代谢及其在K^(+)稳态和抑制甘氨酸甜菜碱转运中的信号传导作用;同时,通过总结现有研究中的乳酸菌c-di-AMP代谢调控,深化对乳酸菌第二信使分子信号调控的认知。 展开更多
关键词 乳酸菌 第二信使分子 环二腺苷酸
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病原细菌受体介导的c-di-GMP信号传导及其调控机制 被引量:3
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作者 杨凤环 田芳 +1 位作者 陈华民 何晨阳 《植物保护》 CAS CSCD 北大核心 2017年第1期9-14,共6页
细菌第二信使环二鸟苷酸(c-di-GMP)信号网络系统主要涉及信号代谢、识别、接受、传递、功能表达和调控。c-di-GMP胞内水平受到鸟苷酸环化酶(DGC)和磷酸二酯酶(PDE)的控制。c-di-GMP信号受体类型多样,包括转录调控因子、PilZ结构域蛋白... 细菌第二信使环二鸟苷酸(c-di-GMP)信号网络系统主要涉及信号代谢、识别、接受、传递、功能表达和调控。c-di-GMP胞内水平受到鸟苷酸环化酶(DGC)和磷酸二酯酶(PDE)的控制。c-di-GMP信号受体类型多样,包括转录调控因子、PilZ结构域蛋白、退化的GGDEF和EAL结构域蛋白、核糖体开关、多核苷酸磷酸化酶和新发现的蛋白激酶等。c-di-GMP受体接受信号后,可以在转录、翻译以及翻译后水平上对下游靶标进行调控,从而影响细菌的毒性、运动性、生物膜形成、细胞分裂等生理生化过程。本文结合本实验室对水稻白叶枯病菌的研究结果,综述了近年来国内外在c-di-GMP信号受体介导的调控机制等方面的研究进展。 展开更多
关键词 c-di-GMP 信号代谢 受体 传导 调控
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外源性C-di-GMP对变形链球菌产酸、耐酸能力的影响 被引量:3
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作者 闫文娟 吴补领 +4 位作者 屈铁军 高杰 赵红萍 苏凌云 余擎 《牙体牙髓牙周病学杂志》 CAS 北大核心 2009年第9期510-512,共3页
目的:初步探讨外源性c-di-GMP对变形链球菌产酸、耐酸能力的影响。方法:外源性c-di-GMP与生理盐水分别加入至含变形链球菌UA159菌液的BHI培养基中,比较两组对变形链球菌产酸、耐酸能力的影响。结果:c-di-GMP处理组与生理盐水组相比,产... 目的:初步探讨外源性c-di-GMP对变形链球菌产酸、耐酸能力的影响。方法:外源性c-di-GMP与生理盐水分别加入至含变形链球菌UA159菌液的BHI培养基中,比较两组对变形链球菌产酸、耐酸能力的影响。结果:c-di-GMP处理组与生理盐水组相比,产酸、耐酸能力明显下降,差异有统计学意义,经t检验,P<0.05。结论:c-di-GMP可以抑制变形链球菌的产酸、耐酸能力,可以单独也可以与其它的抗龋制剂联合应用控制龋病。 展开更多
关键词 c—dj—GMP 变形链球菌 产酸 耐酸
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环二鸟苷酸(c-di-GMP)作为潜在免疫佐剂的研究进展 被引量:1
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作者 丛晓燕 时建立 +6 位作者 孙文博 李俊 杜以军 吴家强 陈蕾 陈智 王金宝 《中国兽药杂志》 2014年第2期59-62,共4页
环二鸟苷酸(cyclic diguanylate,c-di-GMP)是在细菌中普遍存在的第二信使分子,参与调节多种生理功能,近年来研究发现环二鸟苷酸作为免疫调节剂作用于真核细胞可产生良好的免疫调节作用,实验研究表明其有可能成为具有潜力的疫苗佐剂。本... 环二鸟苷酸(cyclic diguanylate,c-di-GMP)是在细菌中普遍存在的第二信使分子,参与调节多种生理功能,近年来研究发现环二鸟苷酸作为免疫调节剂作用于真核细胞可产生良好的免疫调节作用,实验研究表明其有可能成为具有潜力的疫苗佐剂。本文综述了c-di-GMP的结构,生物学功能、免疫特性和作为疫苗佐剂研究等方面的最新研究进展。 展开更多
关键词 环二鸟苷酸 疫苗 佐剂 免疫
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3D-DSA与Dyna-CT在颅内支架置入术中的临床应用研究
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作者 周新华 陈良义 +1 位作者 翁磊华 吕绍茂 《中国CT和MRI杂志》 2024年第1期28-30,共3页
目的探讨三维DSA血管成像(3D-DSA)及数字平板造影机的C臂旋转并断层重建技术(DynaCT)在颅内支架置入术患者中的临床应用。方法选择2019年1月-2020年6月厦门大学附属中山医院收治92例颅内动脉狭窄患者,所有患者均行颅内支架置入术治疗,... 目的探讨三维DSA血管成像(3D-DSA)及数字平板造影机的C臂旋转并断层重建技术(DynaCT)在颅内支架置入术患者中的临床应用。方法选择2019年1月-2020年6月厦门大学附属中山医院收治92例颅内动脉狭窄患者,所有患者均行颅内支架置入术治疗,分为对照组(n=38例)和观察组(n=54例)。对照组采用3D-DSA引导,观察组采用DynaCT引导,术后进行12个月门诊随访,比较两组改良Rankin量表(mRS)评分、手术成功率、并发症、支架再狭窄及斑块检出率。结果观察组术后4个月、8个月及12个月mRS评分、支架成形术耗时低于对照组(P<0.05);观察组Dyna-CT的术前规划、观察到支架表面图像与术中所见吻合,手术成功率为83.33%,高于对照组63.16%(P<0.05);两组围术期并发症发生率无统计意义(P>0.05);观察组手术后进行12个月门诊随访,患者支架再狭窄、血管狭窄部位斑块检出率低于对照组(P<0.05)。结论DynaCT用于颅内支架置入术中能改善患者症状,缩短支架成形术耗时,有助于提高手术成功率,未增加并发症发生率,能降低血管狭窄部位斑块检出率,值得推广应用。 展开更多
关键词 三维DSA血管成像 数字平板造影 c臂旋转并断层重建技术 颅内支架置入术
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基于C-CDIO模式的“数字图像处理”课程教学改革与探讨 被引量:6
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作者 刘伟 郑扬冰 +3 位作者 刘红钊 崔明月 李根全 黄荣杰 《电脑知识与技术》 2015年第12X期98-100,共3页
CDIO体现了最新工程教育理念,是工程教育改革的最新成果。在此基础上,结合"数字图像处理"课程实践性和专业性强的特点,有针对性的进一步发展了以集成化课程链、应用化教学链、协作化实验链为特点的C-CDIO模式来探索"数... CDIO体现了最新工程教育理念,是工程教育改革的最新成果。在此基础上,结合"数字图像处理"课程实践性和专业性强的特点,有针对性的进一步发展了以集成化课程链、应用化教学链、协作化实验链为特点的C-CDIO模式来探索"数字图像处理"课程教学改革。该教学改革打破了传统专业课教学模式,在提高学生工程意识,项目构思、设计、实施和操作的能力,以及团队合作精神等方面取得显著效果。 展开更多
关键词 数字图像处理 c-cdiO 教学改革 自动化 工程教育
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数字技术赋能PBL教学模式创新研究——以C语言程序设计课程为例
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作者 彭琼 辛继湘 《信息与电脑》 2024年第14期19-23,共5页
本文以C语言程序设计课程为例,探讨了数字技术如何赋能以问题为导向的学习(Problem-Based Learning,PBL)的教学模式创新。鉴于C语言程序设计课程在建设过程中,存在课程内容与思政内容融合不紧密、课程与数字技术结合实效性不强等问题,... 本文以C语言程序设计课程为例,探讨了数字技术如何赋能以问题为导向的学习(Problem-Based Learning,PBL)的教学模式创新。鉴于C语言程序设计课程在建设过程中,存在课程内容与思政内容融合不紧密、课程与数字技术结合实效性不强等问题,文章提出了PBL教学模式创新的目标,包括改革教学内容,将价值观的引导作为核心,将能力的提升作为主线,改革教学模式,通过理论教学与实践操作的有机结合,实现教学与育人的相互融合,文章阐述了课程思政融入教学内容、重构教学设计和调整课程评价等实践的过程。实践证明,此模式提高了学习效率,实现了个性化教学,增强了学生参与度,培养了学生自主学习能力和解决问题的能力,加强了学生的实践技能,增强了其团队合作精神。 展开更多
关键词 数字技术赋能 PBL教学模式 c语言程序设计
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基于C-CDIO模式的“数字图像处理”课程教学改革与探讨 被引量:5
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作者 王云峰 《现代信息科技》 2019年第8期99-100,103,共3页
C-CDIO模式是一种专为工程专业课程设计的教育模式。在"数字图像处理"课程教学改革中应用C-CDIO模式,对培养相关专业创新应用型人才有重要作用。本文简单阐述了C-CDIO模式的内涵及对教学改革的意义,通过分析"数字图像处... C-CDIO模式是一种专为工程专业课程设计的教育模式。在"数字图像处理"课程教学改革中应用C-CDIO模式,对培养相关专业创新应用型人才有重要作用。本文简单阐述了C-CDIO模式的内涵及对教学改革的意义,通过分析"数字图像处理"课程的特点以及相关教学中存在的问题,重点讨论了基于C-CDIO模式的"数字图像处理"课程教学改革实践措施,以供诸位参考。 展开更多
关键词 c-cdiO模式 数字图像处理 教育信息化
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美罗培南对胞内不同c-di-GMP浓度表型铜绿假单胞菌生物被膜形成的影响 被引量:5
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作者 谢杰鹏 孔晋亮 +4 位作者 陈一强 王可 罗劲 张真强 刘冬梅 《广西医科大学学报》 CAS 2020年第6期1001-1006,共6页
目的:探究美罗培南对胞内不同环二鸟苷酸(c-di-GMP)浓度表型铜绿假单胞菌(PA)生物被膜形成的影响。方法:两倍稀释法测定美罗培南对各菌株的抑菌浓度(MIC);设阴性对照组和3个美罗培南干预组(1/2MIC、1/4MIC、1/8MIC)。用1/2MIC、1/4MIC、... 目的:探究美罗培南对胞内不同环二鸟苷酸(c-di-GMP)浓度表型铜绿假单胞菌(PA)生物被膜形成的影响。方法:两倍稀释法测定美罗培南对各菌株的抑菌浓度(MIC);设阴性对照组和3个美罗培南干预组(1/2MIC、1/4MIC、1/8MIC)。用1/2MIC、1/4MIC、1/8MIC的美罗培南分别干预野生株PAO1、PA胞内c-di-GMP高表达株(PAO1ΔwspF)、PA胞内c-di-GMP低表达株(PAO1/Plac-yhjH),观察美罗培南对其运动能力的影响,用结晶紫染色法测定美罗培南对PAO1、PAO1ΔwspF、PAO1/Plac-yhjH菌株的生物被膜形成以及对生物被膜清除能力的影响。结果:在美罗培南的干预下,PAO1、PAO1ΔwspF、PAO1/Plac-yhjH的运动区域直径均比阴性对照组小(P<0.05),所形成的的生物被膜也均比阴性对照组少(P<0. 05),而破坏的生物被膜均比阴性对照组多(P<0. 05)。结论:美罗培南能抑制PAO1、PAO1ΔwspF、PAO1/Plac-yhjH的运动能力以及生物被膜形成,美罗培南能破坏PAO1、PAO1ΔwspF、PAO1/Plac-yhjH形成的生物被膜,美罗培南对PAO1、PAO1ΔwspF、PAO1/Plac-yhjH的抑制效果不同。 展开更多
关键词 美罗培南 c-di-GMP 铜绿假单胞菌 生物被膜
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