Digital measuring the ocular morphological parameters of guinea pig eye in vivo with Python

AIM:To quantitatively measure ocular morphological parameters of guinea pig with Python technology.METHODS:Thirty-six eyeballs of eighteen 3-weekold guinea pigs were measured with keratometer and photographed to obtain the horizontal,coronal,and sagittal planes respectively.The corresponding photo pixels-actual length ratio was acquired by a proportional scale.The edge coordinates were identified artificially by ginput function.Circle and conic curve fitting were applied to fit the contour of the eyeball in the sagittal,coronal and horizontal view.The curvature,curvature radius,eccentricity,tilt angle,corneal diameter,and binocular separation angle were calculated according to the geometric principles.Next,the eyeballs were removed,canny edge detection was applied to identify the contour of eyeball in vitro.The results were compared between in vivo and in vitro.RESULTS:Regarding the corneal curvature and curvature radius on the horizontal and sagittal planes,no significant differences were observed among results in vivo,in vitro,and the keratometer.The horizontal and vertical binocular separation angles were 130.6°±6.39°and 129.8°±9.58°respectively.For the corneal curvature radius and eccentricity in vivo,significant differences were observed between horizontal and vertical planes.CONCLUSION:The Graphical interface window of Python makes up the deficiency of edge detection,which requires too much definition in Matlab.There are significant differences between guinea pig and human beings,such as exotropic eye position,oblique oval eyeball,and obvious discrepancy of binoculus.This study helps evaluate objectively the ocular morphological parameters of small experimental animals in emmetropization research.

Vibration parameter measurement using the temporal digital hologram sequence and windowed Fourier transform

Real time digital recording and numerical reconstruction of a temporal digital hologram sequence have become feasible in recent years.They provide a new measurement method which enjoys the valuable advantages of being full-field,noncontact and high precision.In this paper,a combined method of temporal digital hologram sequence and windowed Fourier transform is proposed to measure the kinematic parameters of random vibration.A series of holograms are recorded by CCD camera and the original phase can be reconstructed by Fresnel reconstruction algorithm.The three-dimensional windowed Fourier transform is used to filter noise in phase and extract the instantaneous kinematic parameters of the specimen,such as the displacement,velocity and acceleration.An experiment is conducted on a chloroprene rubber latex membrane.Results demonstrate that the proposed method determines the vibration parameters precisely and enjoys many merits.

A pilot study of the precision of digital intraocular pressure measurement during vitrectomy

AIM:To evaluate the precision of digital intraocular pressure(IOP)measurement in silicone oil(SO)filled eyes during vitrectomy.METHODS:This is a retrospective,single-blind study.Patients who were diagnosed with retinal detachment and scheduled for vitrectomy with SO injection were consecutively enrolled.During the vitrectomy,IOP was digitally measured and then by a rebound tonometer(Icare PRO).The rebound tonometer readings were masked to the surgeons.The digitally measured IOP and that of rebound tonometer were compared,and the inter-methods agreement was assessed.The absolute deviation in IOP values between these two methods(△IOP)was also calculated,and correlations between△IOP and refractive status,lens status and levels of surgeons’experience were analyzed.RESULTS:A total of 131 patients(131 eyes)were recruited,with a mean age of 51.0±16.1 y.There was no significant difference in IOPs between digital measurement and the rebound tonometer(15.6±4.3 vs 15.7±5.1 mm Hg;t=0.406,P=0.686).Intraclass correlation coefficients(ICC)analysis indicated a strong correlation between these two measurements(ICC=0.830,P<0.001).The mean△IOP was 2.0±1.9 mm Hg(range:0-12.8 mm Hg),with 98 eyes(74.8%)had the△IOP within 3 mm Hg.△IOP was found to be negatively correlated with levels of surgeons’experience(r=-0.183;P=0.037),but not with the refractive status or lens status of the patients(both P>0.05).CONCLUSION:For experienced surgeons,the digital IOP measurement may be an acceptable technique for IOP measurement in SO filled eyes during vitrectomy.However,its use by inexperienced surgeons should be taken with caution.

Digital measurement of bone tumor volume by CT three-dimensional reconstruction technology

Objective To discuss the measurement of bone tumor volume on the basis of three dimensional images segmentation technology. Methods Twenty patients with lacunar bone tumor from Tianjin Hospital and Tongji Hospital were included in the

Spectral Measurement of Atmospheric Pressure Plasma by Means of Digital Camera

A digital camera measuring system has been used successfully to measure the space fluctuation behaviors of Induced Dielectric Barrier Discharge (IDBD) plasma at atmospheric pres- sure. The experimental results showed that: (1) The uniformity of electron temperature in space depends on discharge condition and structure of web electrode. For a certain web electrode the higher the discharge voltage is, the more uniform distribution of electron temperature in space will be. For a certain discharge the finer and denser the holes on web electrode are, the more uniform distribution of electron temperature in space will be (2). Digital camera is an available equipment to measure some behaviors of the plasma working at atmospheric pressure.

A convenient look-up-table based method for the compensation of non-linear error in digital fringe projection

Although the structured light system that uses digital fringe projection has been widely implemented in three-dimensional surface profile measurement, the measurement system is susceptible to non-linear error. In this work, we propose a convenient look-up-table-based （LUT-based） method to compensate for the non-linear error in captured fringe patterns. Without extra calibration, this LUT-based method completely utilizes the captured fringe pattern by recording the full-field differences. Then, a phase compensation map is established to revise the measured phase. Experimental results demonstrate that this method works effectively.

Subset-based local vs.finite element-based global digital image correlation:A comparison study

Being the two primary approaches for full-field kinematics measurements, both subset-based local digital image correlation （DIC） and finite element-based global DIC have been extensively studied. Nowadays, most commercial DIC systems employ local DIC algorithm because of its advantages of straight forward principle and higher efficiency. However, several researchers argue that global DIC can provide better displacement results due to the displacement continuity constraint among adjacent elements. As such, thoroughly examining the performance of these two different DIC methods seems to be highly necessary. Here, the random errors associated with local DIC and two global DIC methods are theoretically analyzed at first. Subsequently, based on the same algorithmic details and parameters during analyses of numerical and real experiments, the performance of the different DIC approaches is fairly compared. Theoretical and experimental results reveal that local DIC outperforms its global counterpart in terms of both displacement results and computational efficiency when element （subset） size is no less than 11 pixels.

Employment of predictive search algorithm in digital image correlation

A predictive search algorithm to estimate the size and direction of displacement vectors was presented.The algorithm decreased the time of calculating the displacement of each pixel.In addition,the updating reference image scheme was used to update the reference image and to decrease the computation time when the displacement was larger than a certain number.In this way,the search range and computational complexity were cut down,and less EMS memory was occupied.The capability of proposed search algorithm was then verified by the results of both computer simulation and experiments.The results showed that the algorithm could improve the efficiency of correlation method and satisfy the accuracy requirement for practical displacement measuring.

Advances in shape measurement in the digital world

The importance of particle shape in terms of its effects on the behaviour of powders and other particulate systems has long been recognised, but particle shape information has been rather difficult to obtain and use until fairly recently, unlike its better-known counterpart, particle size. However, advances in computing power and 3D image acquisition and analysis techniques have resulted in major progress being made in the measurement, description and application of particle shape information in recent years. Because we are now in a digital era, it is fitting that many of these advanced techniques are based on digital technology. This review article aims to trace the development of these new techniques, highlight their contributions to both academic and practical applications, and present a perspective for future developments.

Measurement of in-plane deformations of microsystems by digital holography and speckle interferometry(Invited Paper)

The reliability of microsystems is an important issue and for their quality inspection, it is necessary to know the displacements or deformations due to the applied mechanical, thermal, or electrostatic loads. We show how interferometrical techniques like digital holography and speckle interferometry can be used for the measurement of in plane deformations of microsystems with nanometric accuracy and we give a description of the measurement uncertainties.

Measurement of out-of-plane deformation of curved objects with digital speckle pattern interferometry

Digital speckle pattern interferometry （DSPI） is a high-precision deformation t technique for planar objects. However, for curved objects, the three-dimensional （3D） shape information is needed in order to obtain correct deformation measurement in DSPI. Thus, combined shape and deformation measurement techniques of DSPI have been proposed. However, the current techniques are either complex in setup or complicated in operation. Furthermore, the operations of some techniques are too slow for real-time measurement. In this work, we propose a DSPI technique for both 3D shape and out-of-plane deformation measurement. Compared with current techniques, the proposed technique is simple in both setup and operation and is capable of fast deformation measurement. Theoretical analysis and experiments are performed. For a cylinder surface with an arch height of 9 mm, the error of out-of-plane deformation measurement is less than 0.15 μm. The effectiveness of the proposed scheme is verified.

The new era for geo-information

Along with the forthcoming of Google Earth, Virtual Earth, the next generation of Internet, Web 2.0, grid computing and smart sensor web, comes the new era for geo-information. In this paper, main features of new geo-information era are discussed. This new era is characterized by these features： serviced users are extended from professionals to all public users, the users are data and information providers as well, geospatial data provided are no longer measurement-by-specification but measurement-on- demand through smart sensor web, and services are transferred from being data-driven to application- driven. Such problems as out-of-order issues in geographic data collection and information prolifer- ation, quality issues in geographic information updating, security issues in geographic information services, privacy issues in sharing geographic information and property issues in sharing geographic information, which are brought about by new geo-information era, especially those confronted in geo- information science and geo-spatial information industry, are analyzed. Then strategies concerning standards, planning, laws, technology and applications are proposed.

Experimental and numerical study on structural performance of reinforced concrete box sewer with localized extreme defect

An experimental and numerical investigation into the structural performance of reinforced concrete box sewers with typical corrosion-related extreme defects localized at the ceiling was conducted.Firstly,during the large-scale laboratory test,some key struc-tural responses were captured and evaluated,including the crack width development process(via digital image correlation measurement),ceiling deflection,and material strains of both complete and typical defective boxes.The failure modes and load-carrying mechanism throughout the specimen loading phases were analyzed.Furthermore,the specimen failure process was simulated using a damage-basedfinite element method,and a related parameter sensitivity analysis was performed.The results indicate that the defective ceiling cracked at mid-span under a low load value,but the bending capacity loss can be substituted by two shoulders and carry three tofive times more load before completely collapsing.The simulation matched the lab test qualitatively,and with the suggested set strategy of material parameters,the load-deflection feature curve could provide a practical prediction of the ultimate bearing capacity of the defec-tive sewers,with a 10–15%error on the safe side.

High-precision method for measuring the photothermal properties of transparent media with digital holography

Quantitative phase microscopy by digital holography provides direct access to the phase profile of a transparent subject with high precision. This is useful for observing phenomena that modulate phase, but are otherwise difficult or impossible to detect. In this letter, a carefully constructed digital holographic apparatus is used to measure optically induced thermal lensing with an optical path difference precision of less than 1 nm. Furthermore, by taking advantage of the radial symmetry of a thermal lens, such data are processed to determine the absorption coefficient of transparent media with precisions as low as 1 × 10-5cm-1 using low power （30 mW） continuous wave （CW） excitation.