Modeling and validating three dimensional human normal cervix and cervical cancer tissues in vitro

The use of three dimensional in vitro systems in cancer research is a promising path for developing effective anticancer therapies.The aim of this study was to engineer a functional 3-D in vitro model of normal and cancerous cervical tissue.Normal epithelial and immortalized cervical epithelial carcinoma cell lines were used to construct 3-D artificial normal cervical and cervical cancerous tissues.De-epidermised dermis（DED） was used as a scaffold for both models.Morphological analyses were conducted by using hematoxylin and eosin staining and characteristics of the models were studied by analyzing the expression of different structural cytokeratins and differential protein marker MAX dimerisation protein 1（Mad1） using immunohistochemical technique.Haematoxylin and eosin staining results showed that normal cervical tissue had multi epithelial layers while cancerous cervical tissue showed dysplastic changes.Immunohistochemistry staining revealed that for normal cervix model cytokeratin 10 was expressed in the upper stratified layer of the epithelium while cytokeratin 5 was expressed mainly in the middle and basal layer.Cytokeratin 19 was weakly expressed in a few basal cells.Cervical cancer model showed cytokeratin 19 expression in different epithelial layers and weak or no expression for cytokeratin 5 and cytokeratin 10.Madl expression was detected in some suprabasal cells.The 3-D in vitro models showed stratified epithelial layers and expressed the same types and patterns of differentiation marker proteins as seen in corresponding in vivo tissue in either normal cervical or cervical cancerous tissue.These findings imply that they can serve as functional normal and cervical cancer models.

Teaching Reform and Practice in Engineering Drawing Based on 3D Modeling with Computer

Based on the necessity of three dimensional modeling with computer in teaching reform, this paper is the summarization of reform practice of teaching engineering drawing in our institute. The teaching reform begins with three dimensional modeling that used computer instead of board. On the basis of target of teaching reform, set of teaching content, arrangement of class hour and teaching method, the research of teaching practice have been done, and very good effects in teaching of engineering drawing have been achieved.

Model-based Predictive Control for Spatially-distributed Systems Using Dimensional Reduction Models

In this paper, a low-dimensional multiple-input and multiple-output （MIMO） model predictive control （MPC） configuration is presented for partial differential equation （PDE） unknown spatially-distributed systems （SDSs）. First, the dimension reduction with principal component analysis （PCA） is used to transform the high-dimensional spatio-temporal data into a low-dimensional time domain. The MPC strategy is proposed based on the online correction low-dimensional models, where the state of the system at a previous time is used to correct the output of low-dimensional models. Sufficient conditions for closed-loop stability are presented and proven. Simulations demonstrate the accuracy and efficiency of the proposed methodologies.

Numerical simulation on fluid flow behavior during 3-dimensional dendrite growth with random preference angle

The flow behavior of three-dimensional(3D)dendrite growth with random preferred angle under natural convection was studied by using the Lattice Boltzmann-Cellular Automata(LB-CA)method with dynamic and static grids.In this model,the temperature field,flow field and solute field calculated by Lattice Boltzmann method(LBM)and dendrite growth calculated by CA method were carried out in static and dynamic grids respectively,and the coupling between LBM and CA was performed by interpolation of calculation parameters between dynamic and static grids.Results show that the asymmetry of solid phase distribution makes the streamline distribution more complex.At the initial stage of multiple dendrites growth,the fluid flow is relatively free.When dendrites grow close to each other,the fluid flow is blocked and can only flow along the gap between dendrites.During the wall equiaxed-columnar-central equiaxed crystals transformation(ECET)process,dense eddy current is formed at the wall equiaxed crystals at first.Then,when the wall equiaxed crystals gradually develop into columnar crystals,the eddy current moves with the solid-liquid interface.When the central equiaxed crystals are formed,the eddy current at the front of the columnar crystals gradually disappears.New eddies appear as the central equiaxed crystal grows.

Seismic data reconstruction based on low dimensional manifold model

Seismic data reconstruction is an essential and yet fundamental step in seismic data processing workflow,which is of profound significance to improve migration imaging quality,multiple suppression effect,and seismic inversion accuracy.Regularization methods play a central role in solving the underdetermined inverse problem of seismic data reconstruction.In this paper,a novel regularization approach is proposed,the low dimensional manifold model(LDMM),for reconstructing the missing seismic data.Our work relies on the fact that seismic patches always occupy a low dimensional manifold.Specifically,we exploit the dimension of the seismic patches manifold as a regularization term in the reconstruction problem,and reconstruct the missing seismic data by enforcing low dimensionality on this manifold.The crucial procedure of the proposed method is to solve the dimension of the patches manifold.Toward this,we adopt an efficient dimensionality calculation method based on low-rank approximation,which provides a reliable safeguard to enforce the constraints in the reconstruction process.Numerical experiments performed on synthetic and field seismic data demonstrate that,compared with the curvelet-based sparsity-promoting L1-norm minimization method and the multichannel singular spectrum analysis method,the proposed method obtains state-of-the-art reconstruction results.

A three-dimensional model for the transportation of Fe and Mn in Arha Reservoir

Coupling with a three dimensional (3D) hydrodynamic model and a suspended solids model, a 3D model for the transport of Fe and Mn in Arha Reservoir, China, was developed. The 3D velocity fields for the flood season are computed to drive the 3D model of Fe and Mn in which the processes of advection, diffusion, redox, sorption, desorption, deposition, and re suspension are included. The model has been calibrated by matching observed fluid, suspended solids, and total concentrations of Fe and Mn in the water column and in the sediment, successively. The model simulated both horizontal and vertical gradients of Fe and Mn in Arha Reservoir. It was found that Fe and especially Mn stratify in accordance with the stratification of DO during summer. The redox cycles across the water sediment interface has a principal role in the rise of Fe and Mn concentrations in the overlying water. It was also found that Fe and Mn loadings from the tributaries have a carryover effect on the water quality through a secondary contamination in the reservoir.

Thermal fluctuation conductivity and dimensionality in iron-based superconductors

The time-dependent Ginzburg–Landau Lawrence–Doniach model is used to investigate the superconducting fluctuation electrical conductivities.The theoretical result based on the self-consistent Gaussian approximation is used to fit the transport measurement data of iron-based superconductors F-doped La OFe As and Ba Fe_（2-x）Ni_xAs_2.We demonstrate that La OFe As shows layered behavior,while Ba Fe_（2-x）Ni_xAs_2 is more of a 3D feature.The conductivity in the region near Tc is well described by the theoretical formula.

Preoperative detection of intrahepatic venovenous shunt treated by microwave precoagulation during right hepatectomy

A 53-year-old woman underwent a 2-stage right hepatectomy for bilobar metastasis of an ileal neuroendocrine carcinoma. Preoperative three-dimensional computed tomography reconstruction helped to diagnose an intrahepatic venovenous shunts from the right and middle hepatic veins to the left hepatic vein, which could cause a intraoperative bleeding. Hemostasis was performed by means of precoagulation with microwaveassisted coagulation.

Three-Dimension Mathematical Model of Total Phosphor in the Reservoir and Application

Taking the transport of total phosphor pollutants in the Beijing Miyun reservoir for example,we have obtained three dimensional distributing regularity of total phosphor pollutants by the calculation of the linear interpolation value of each point between horizontal layers. The credibility analysis in allusion to this method was carried out and the programming scheme for realizing this method was set forth.

Exploration of nature-based biomimetic approach in landscape architectural design: parametric study of candelabra model design

This research provides an exploration of a biomimetic approach in the process of designing a candelabra model using linear shaped leaves of a Bell flower.The design process described in this research contains two steps:biological and geometrical.In the first biological step,a proper model for the creation of an urban element was found from nature in a Bell flower(Campanula persicifolia L.).The upper leaves of the selected plant,which are small with a linear spear and sharpening at the top,were chosen for the modeling process.The second step included applying two geometrical methods,i.e.,Voronoi diagrams and Delaunay triangulation.A geometrical leaf form of the selected plant species and the modeling process were obtained using aparametric modeling software,Blender.Using different Blender plug-ins and modifiers,Delaunay triangulation and Voronoi diagram were implemented by marking the starting points on the leaf form in the image data source,adjusting the Delaunay triangulation parameters,and creating Voronoi diagrams in which the Voronoi points were located at the shortest distance from the edges of the Voronoi polygon.Consequently,a three dimensional model of a candelabra was developed through this study.

Molecular Exchanging Energy of Anionic/Cationic Surfactants System on the Surface of Solution

In order to study synergism of the mixed surfactants system with molecular exchanging energy (E), the Lennard-Jones formula has been firstly introduced to evaluate the E of the mixed system, CH3(CH2)nOSO 3 /CH3(CH2)nN+(CH3)3 directly from their molecular structure. The comparison of the calculated and the observed results showed that this method is practical.

2D Coupled 3D:A New Numerical Model for Dual - Structured - Aquifer System

This paper points out that a successful numerical simulation is to construct a correct conceptional model which is very dose to the natural condition. A new model, two dimensional coupled three dimensional model (2D -3D ) is presented in the Present paper,which is the most suitable one for the dual - structured - aquifer system. An example of Wenyinghu area is shown.By using the 2D-3D model, a satisfied result of the simulated area is achieved.

Common Management Process Model of New TQM Based on the Situation Analysis

In the previous study, we suggested the concept of new TQM based on the consideration of basic concept of Quality Control. Also, in the previous study, we suggested the target domains and entities of product and process based on the TQM Matrix and view point of Three Dimensional Unification Value Models for managing quality of organization systems. Furthermore, in the previous study, we suggest the Common Management Process of organizations. Based on the above suggestion, in this paper, we would like to propose the Common Management Process Model of Total Quality Management based on the consideration of situation analysis and more precise definition of TQM Matrix and Three Dimensional Unification Value Model of “Product and Process”. Improvement of quality and efficiency of organization management can be expected by the integration of conventional different management such as quality assurance, quality improvement, risk management, investment individually from the view point of common management process.

Concept and evaluation of bay health:the role of numerical model in the Yueqing Bay,China

To better evaluate the three-dimensional bay health and predict the dynamic bay health conditions, a concept of numerical bay health was introduced and a method of numerical bay health evaluation（NBHE） was developed.To support the NBHE method, a numerical bay health index（NBHI） system was constructed, which assess the natural and socio-economic effects on the entire bay. Five index groups are combined to formulate the NBHI,including geometry, hydrodynamics and sediment dynamics, bio-ecology, water quality and socio-economy.Each group has different number of indices selected and weighted using AHP method according to their importance. Data were mainly synthesized from a variety of numerical models together with monitoring programs, which provide superior to other approaches in discriminating data integrity and predicting data in future. The NBHE method using NBHI system was applied in the Yueqing Bay during spring tide in April 2007.According to the NBHE results, Sta. A, at the surface level of the estuarine mouth, has a healthy geometry condition, sub-healthy hydrodynamic and sediment dynamic condition, and unhealthy water quality and bioecology conditions. The integrated healthy score at Sta. A indicates its sub-healthy condition.

FINITE-ELEMENT MODELLING OF SOIL-GEOGRID INTERACTION DEALING WITH THE PULLOUT BEHAVIOUR OF GEOGRIDS

To predict the behavior of geogrids embedded in sand under pullout loading conditions, the two dimensional plane-stress finite element model was presented. The interactions between soil and geogrid were simulated as non-linear springs, and the stiffness of the springs was determined from simple tests in the specially designed pullout box. The predicted behavior of the geogrid under pullout load agrees well with the observed data including the load-displacement properties, the displacement distribution along the longitudinal direction and the mobilization of the frictional and bearing resistance. (Edited author abstract) 8 Refs.

RCV-based error density estimation in the ultrahigh dimensional additive model

In this paper,we mainly study how to estimate the error density in the ultrahigh dimensional sparse additive model,where the number of variables is larger than the sample size.First,a smoothing method based on B-splines is applied to the estimation of regression functions.Second,an improved two-stage refitted crossvalidation(RCV)procedure by random splitting technique is used to obtain the residuals of the model,and then the residual-based kernel method is applied to estimate the error density function.Under suitable sparse conditions,the large sample properties of the estimator,including the weak and strong consistency,as well as normality and the law of the iterated logarithm,are obtained.Especially,the relationship between the sparsity and the convergence rate of the kernel density estimator is given.The methodology is illustrated by simulations and a real data example,which suggests that the proposed method performs well.

A three-dimensional solid-liquid two-phase turbulence model with the effect of vegetation in non-orthogonal curvilinear coordinates

A three-dimensional k-ε-Ap solid-liquid two-phase two-fluid model with the effect of vegetation is solved numerically with a finite-volume method on an adaptive grid to study water-sediment movements and bed evolution in vegetated channels. The additional drag force and additional turbulence generation due to vegetation are added to the relevant control equations for simulating the interaction between vegetation and flow. The flow structure and the bed-topography changes in a 60° partly vegetated channel bend are calculated by the model. The numerical results agree well with the measured ones. Calculated and measured results show that the primary flow velocity reduces much in the vegetation zone and increases in the non-vegetation zone, the secondary flow velocity weakens in the vegetation zone and strengthens in the non-vegetation zone, the sediment movement and bed-topography change also weaken in the vegetation zone and strengthen in the non-vegetation zone, a well-planed vegetation arrangement can improve bank stabilization program, and the k-ε-Ap model can deal with bed-load transport with a more reasonable method than the one-fluid model.

A REAL-TIME MATHEMATICAL MODEL FOR TWO DIMENSIONAL TIDAL FLOW AND WATER QUALITY

A real-time mathematical model for two dimensional tidal flow and water quality is presented in this paper. The control-volume-based-finite-difference method and the 'power interpolation distribution' advocated by Patankar [4] have been employed, and new boundary condition for tidal flow is recommended. The model is un- conditionally stable and convergent, and able to deal with irregular estuarine topography and movable boundary problems. Practical application of the model is illustrated by an example for the Swatou Bay. A fair agreement be- tween the values measured and computed demonstrates the validity of the model developed.

Optimization of fused deposition modeling process parameters using a fuzzy inference system coupled with Taguchi philosophy

Fused deposition modeling （FDM） is an additive manufacturing technique used to fabricate intricate parts in 3D, within the shortest possible time without using tools, dies, fixtures, or human intervention. This article empiri- cally reports the effects of the process parameters, i.e., the layer thickness, raster angle, raster width, air gap, part orientation, and their interactions on the accuracy of the length, width, and thicknes, of acrylonitrile-butadiene- styrene （ABSP 400） parts fabricated using the FDM tech- nique. It was found that contraction prevailed along the directions of the length and width, whereas the thickness increased from the desired value of the fabricated part. Optimum parameter settings to minimize the responses, such as the change in length, width, and thickness of the test specimen, have been determined using Taguchi＇s parameter design. Because Taguchi＇s philosophy fails to obtain uniform optimal factor settings for each response, in this study, a fuzzy inference system combined with the Taguchi philosophy has been adopted to generate a single response from three responses, to reach the specific target values with the overall optimum factor level settings. Further, Taguchi and artificial neural network predictive models are also presented in this study for an accuracy evaluation within the dimensions of the FDM fabricated parts, subjected to various operating conditions. The pre- dicted values obtained from both models are in good agreement with the values from the experiment data, with mean absolute percentage errors of 3.16 and 0.15, respectively. Finally, the confirmatory test results showed an improvement in the multi-response performance index of 0.454 when using the optimal FDM parameters over the initial values.

Approximation to the Distribution of the Least Squares Estimators in Two Dimensional Cosine Models by Randomly Weighted Bootstrap

Recently, Kundu and Gupta （Metrika, 48：83 C 97, 1998） established the asymptotic normality of the least squares estimators in the two dimensional cosine model. In this paper, we give the approximation to the general least squares estimators by using random weights which is called the Bayesian bootstrap or the random weighting method by Rubin （Annals of Statistics, 9：130 C 134, 1981） and Zheng （Acta Math. Appl. Sinica （in Chinese）, 10（2）： 247 C 253, 1987）. A simulation study shows that this approximation works very well.