Presently the gold standard diagnostic technique for rabies is the direct immunofluorescence assay ( dFA) which is very expensive and requires a high level of expertise. There is a need for more economical and user fr...Presently the gold standard diagnostic technique for rabies is the direct immunofluorescence assay ( dFA) which is very expensive and requires a high level of expertise. There is a need for more economical and user friendly tests, particularly for use in developing countries. We have established one such test called the direct rapid immunohistochemical test (dRIT) for diagnosis of rabies using brain tissue. The test is based on capture of rabies nucleoprotein (N) antigen in brain smears using a cocktail of biotinylated monoclonal antibodies specific for the N protein and color development by streptavidin peroxidase-amino ethyl carbazole and counter staining with haematoxollin. The test was done in parallel with standard FAT dFA using 400 brain samples from different animals and humans. The rabies virus N protein appears under light microscope as reddish brown particles against a light blue background. There was 100 % correlation between the results obtained by the two tests. Also, interpretation of results by dRIT was easier and only required a light microscope. To conclude, this newly developed dRIT technique promises to be a simple, cost effective diagnostic tool for rabies and will have applicability in field conditions prevalent in developing countries.展开更多
The Schwartz technique is a simple method for labeling McAb directly with ￣(99m)Tc and can he utilized to design a one-step radiopharmacelltical kit.In this study,it was applied to label anti-human gastric cancer McA...The Schwartz technique is a simple method for labeling McAb directly with ￣(99m)Tc and can he utilized to design a one-step radiopharmacelltical kit.In this study,it was applied to label anti-human gastric cancer McAb 3H11. The antibody was reduced at room temperature for 30 min with a 1000-fold molar ratio excess of 2mercaptoethanol. The mean labeling efficiency of ￣(99m)Tc3H11 was more than 95%. The immunoreactivity of ￣(99m)Tc-3H11 was more than 80% by ELISA's method. Competition results in vitro showed that ￣(99m)Tc was combined with the high affinity sites of antibody by HPLC. The biodistribution in nude mice bearing 823 gastric cancer xenographts showed that the radioactivity of tumor uptake at 24 h post-injection was the highest except for kidney.The tumor uptake was 8.98±2.42% i.d/g.The count ratio of tumor to blood was over 1.5 and that tumor to liver was more than 2.5 at 24 h post-injection.And the tumor was clearly imaged at 22 h post-injection. The initial clinical results showed that￣(99m)Tc-3H11 was stable in vivo and well good located at the lesion sites.展开更多
目的分析在新生儿溶血病(hemolytic disease of the fetus and newborn,HDFN)中血清学检测结果的应用价值。方法选取2019年1月—2022年12月在龙岩市第二医院新生儿科接受治疗的高胆红素血症患儿100例,对其进行抗体释放试验、游离抗体试...目的分析在新生儿溶血病(hemolytic disease of the fetus and newborn,HDFN)中血清学检测结果的应用价值。方法选取2019年1月—2022年12月在龙岩市第二医院新生儿科接受治疗的高胆红素血症患儿100例,对其进行抗体释放试验、游离抗体试验、直接抗人球蛋白试验、不规则抗体筛查、血型鉴定检测,对比产前母体采血送检结果与产后婴儿采血送检结果差异。结果100例患儿中,58例患儿出现母婴血型不合,其中ABO血型系统50例,Rh血型系统8例。ABO血型系统HDFN发生率显著高于Rh血型系统(P<0.05);50例ABO血型系统母婴不合者中,O-A型HDFN发生率显著高于O-B型(P<0.05)。31例HDFN患儿的血清学检测结果中,单独抗体放散试验为阳性的HDFN患儿占比率显著高于其他类型(P<0.05)。产前检测IgG抗A或抗B效价结果与产后采血对比,产前检测准确率为90.32%。结论血清学检测结果在HDFN诊断中具有一定的辅助价值,在1周内检测阳性率最高。展开更多
目的对疑似新生儿溶血病(hemolytic disease of newborn,HDN)患儿进行溶血三项的筛查,了解HDN的检出率在患儿血型、性别、出生日龄以及母亲妊娠次数的分布情况。方法收集淄博市妇幼保健院402例疑似ABO-HDN患儿及其母亲的血液标本,进行...目的对疑似新生儿溶血病(hemolytic disease of newborn,HDN)患儿进行溶血三项的筛查,了解HDN的检出率在患儿血型、性别、出生日龄以及母亲妊娠次数的分布情况。方法收集淄博市妇幼保健院402例疑似ABO-HDN患儿及其母亲的血液标本,进行血型鉴定、母亲进行不规则抗体筛查、患儿进行溶血三项试验(直接抗人球蛋白试验、游离抗体试验及放散试验),并对检测结果进行分析。结果在402例疑似ABO-HDN患儿中,证实为该病的有222例,占55.22%。A型和B型患儿ABO-HDN阳性率分别为62.86%、49.34%;男性患儿与女性患儿ABO-HDN的阳性率分别为48.06%、63.73%;出生日龄≤1 d的患儿阳性率为77.23%;2~3 d的患儿阳性率为57.46%;>3 d的患儿阳性率为33.33%;母亲第一胎与非第一胎ABO-HDN的阳性率分别为47.40%、61.14%。结论不同血型、性别的患儿,ABO-HDN的阳性检出率有一定差别;患儿的出生日龄以及患儿母亲怀孕次数的不同,ABO-HDN的阳性检出率也有一定差别。展开更多
文摘Presently the gold standard diagnostic technique for rabies is the direct immunofluorescence assay ( dFA) which is very expensive and requires a high level of expertise. There is a need for more economical and user friendly tests, particularly for use in developing countries. We have established one such test called the direct rapid immunohistochemical test (dRIT) for diagnosis of rabies using brain tissue. The test is based on capture of rabies nucleoprotein (N) antigen in brain smears using a cocktail of biotinylated monoclonal antibodies specific for the N protein and color development by streptavidin peroxidase-amino ethyl carbazole and counter staining with haematoxollin. The test was done in parallel with standard FAT dFA using 400 brain samples from different animals and humans. The rabies virus N protein appears under light microscope as reddish brown particles against a light blue background. There was 100 % correlation between the results obtained by the two tests. Also, interpretation of results by dRIT was easier and only required a light microscope. To conclude, this newly developed dRIT technique promises to be a simple, cost effective diagnostic tool for rabies and will have applicability in field conditions prevalent in developing countries.
文摘The Schwartz technique is a simple method for labeling McAb directly with ￣(99m)Tc and can he utilized to design a one-step radiopharmacelltical kit.In this study,it was applied to label anti-human gastric cancer McAb 3H11. The antibody was reduced at room temperature for 30 min with a 1000-fold molar ratio excess of 2mercaptoethanol. The mean labeling efficiency of ￣(99m)Tc3H11 was more than 95%. The immunoreactivity of ￣(99m)Tc-3H11 was more than 80% by ELISA's method. Competition results in vitro showed that ￣(99m)Tc was combined with the high affinity sites of antibody by HPLC. The biodistribution in nude mice bearing 823 gastric cancer xenographts showed that the radioactivity of tumor uptake at 24 h post-injection was the highest except for kidney.The tumor uptake was 8.98±2.42% i.d/g.The count ratio of tumor to blood was over 1.5 and that tumor to liver was more than 2.5 at 24 h post-injection.And the tumor was clearly imaged at 22 h post-injection. The initial clinical results showed that￣(99m)Tc-3H11 was stable in vivo and well good located at the lesion sites.
基金Supported by the Ministry of Scientific Research, Academy of Scientific Research and Technology, Medical Research Council Code: P5-MED-030-01US-Egypt joint project BIO7-002-011
文摘目的分析在新生儿溶血病(hemolytic disease of the fetus and newborn,HDFN)中血清学检测结果的应用价值。方法选取2019年1月—2022年12月在龙岩市第二医院新生儿科接受治疗的高胆红素血症患儿100例,对其进行抗体释放试验、游离抗体试验、直接抗人球蛋白试验、不规则抗体筛查、血型鉴定检测,对比产前母体采血送检结果与产后婴儿采血送检结果差异。结果100例患儿中,58例患儿出现母婴血型不合,其中ABO血型系统50例,Rh血型系统8例。ABO血型系统HDFN发生率显著高于Rh血型系统(P<0.05);50例ABO血型系统母婴不合者中,O-A型HDFN发生率显著高于O-B型(P<0.05)。31例HDFN患儿的血清学检测结果中,单独抗体放散试验为阳性的HDFN患儿占比率显著高于其他类型(P<0.05)。产前检测IgG抗A或抗B效价结果与产后采血对比,产前检测准确率为90.32%。结论血清学检测结果在HDFN诊断中具有一定的辅助价值,在1周内检测阳性率最高。
