The Schwartz technique is a simple method for labeling McAb directly with ￣(99m)Tc and can he utilized to design a one-step radiopharmacelltical kit.In this study,it was applied to label anti-human gastric cancer McA...The Schwartz technique is a simple method for labeling McAb directly with ￣(99m)Tc and can he utilized to design a one-step radiopharmacelltical kit.In this study,it was applied to label anti-human gastric cancer McAb 3H11. The antibody was reduced at room temperature for 30 min with a 1000-fold molar ratio excess of 2mercaptoethanol. The mean labeling efficiency of ￣(99m)Tc3H11 was more than 95%. The immunoreactivity of ￣(99m)Tc-3H11 was more than 80% by ELISA's method. Competition results in vitro showed that ￣(99m)Tc was combined with the high affinity sites of antibody by HPLC. The biodistribution in nude mice bearing 823 gastric cancer xenographts showed that the radioactivity of tumor uptake at 24 h post-injection was the highest except for kidney.The tumor uptake was 8.98±2.42% i.d/g.The count ratio of tumor to blood was over 1.5 and that tumor to liver was more than 2.5 at 24 h post-injection.And the tumor was clearly imaged at 22 h post-injection. The initial clinical results showed that￣(99m)Tc-3H11 was stable in vivo and well good located at the lesion sites.展开更多
Objective: To explore the law and role of apoptosis inducing effect of allicin on human acute T-lymphocyte leukemia cell line (6T-CEM).Methods: Terminal deoxylnucleotide transferase directed X-UTP nick and end labelin...Objective: To explore the law and role of apoptosis inducing effect of allicin on human acute T-lymphocyte leukemia cell line (6T-CEM).Methods: Terminal deoxylnucleotide transferase directed X-UTP nick and end labeling (TUNEL) assay, DNA gel electrophoresis, light microscopy and revers transcription-polymerase chair reaction (RT-PCR) were used to observe apoptosis of cells.Results: Allicin showed significant inducing effect on apoptosis of 6T-CEM cell when the concentration was 0.1 μg/ml, reaching its peak effect when the concentration was 50 μg/ml, with the apoptosis rate as 36.4%. Allicin (15 μg/ml) acted on 6T-CEM for 2 h and induced obvious cell apoptosis, the highest effect reached after 24 h of action, with the apoptosis rate as 56.9%. Actidione or actinomycin D could induce apoptosis of 6T-CEM, the allicin could not enhance their apoptosis inducing effect. Allicin could cause significant reduction of bcl-2 and bax gene expression of 6T-CEM, especially the bcl-2.Conclusion: Allicin could induce cell apoptosis of 6T-CEM with significant time and dose effect. Mechanism of the effect is through inhibiting the bcl-2 gene expression to reduce bcl-2/bax ratio.展开更多
文摘The Schwartz technique is a simple method for labeling McAb directly with ￣(99m)Tc and can he utilized to design a one-step radiopharmacelltical kit.In this study,it was applied to label anti-human gastric cancer McAb 3H11. The antibody was reduced at room temperature for 30 min with a 1000-fold molar ratio excess of 2mercaptoethanol. The mean labeling efficiency of ￣(99m)Tc3H11 was more than 95%. The immunoreactivity of ￣(99m)Tc-3H11 was more than 80% by ELISA's method. Competition results in vitro showed that ￣(99m)Tc was combined with the high affinity sites of antibody by HPLC. The biodistribution in nude mice bearing 823 gastric cancer xenographts showed that the radioactivity of tumor uptake at 24 h post-injection was the highest except for kidney.The tumor uptake was 8.98±2.42% i.d/g.The count ratio of tumor to blood was over 1.5 and that tumor to liver was more than 2.5 at 24 h post-injection.And the tumor was clearly imaged at 22 h post-injection. The initial clinical results showed that￣(99m)Tc-3H11 was stable in vivo and well good located at the lesion sites.
文摘Objective: To explore the law and role of apoptosis inducing effect of allicin on human acute T-lymphocyte leukemia cell line (6T-CEM).Methods: Terminal deoxylnucleotide transferase directed X-UTP nick and end labeling (TUNEL) assay, DNA gel electrophoresis, light microscopy and revers transcription-polymerase chair reaction (RT-PCR) were used to observe apoptosis of cells.Results: Allicin showed significant inducing effect on apoptosis of 6T-CEM cell when the concentration was 0.1 μg/ml, reaching its peak effect when the concentration was 50 μg/ml, with the apoptosis rate as 36.4%. Allicin (15 μg/ml) acted on 6T-CEM for 2 h and induced obvious cell apoptosis, the highest effect reached after 24 h of action, with the apoptosis rate as 56.9%. Actidione or actinomycin D could induce apoptosis of 6T-CEM, the allicin could not enhance their apoptosis inducing effect. Allicin could cause significant reduction of bcl-2 and bax gene expression of 6T-CEM, especially the bcl-2.Conclusion: Allicin could induce cell apoptosis of 6T-CEM with significant time and dose effect. Mechanism of the effect is through inhibiting the bcl-2 gene expression to reduce bcl-2/bax ratio.
