Effects of Temperature on Determination of Protein Concentration with Coomassie Brilliant Blue Method

[Objective] This study aimed to investigate the effects of temperature on determination of protein concentration with Coomassie Brilliant Blue method,thus proving advice and guidance for accurate determination of protein concentration.[Method] With Coomassie Brilliant Blue method,the concentrations of different bovine serum albumin samples were determined under different temperatures and incubation time.[Result] According to the standard curve,when the determination range of protein concentration was 0-100 mg/ml,the determined protein concentration was relatively stable after incubation at 20 ℃ for 20-30 min.Furthermore,the determination result of higher protein concentration with Coomassie Brilliant Blue method was less affected by various factors.[Conclusion] In determination of protein concentration with Coomassie Brilliant Blue method,temperature,sample concentration and incubation time were important factors affecting the accuracy of experimental results.

Synthesis of Water Utilization System Using Concentration Interval Analysis Method （ Ⅰ ） Non-Mass-Transfer-Based Operation

A strategy for water and wastewater minimization is developed for continuous water utilization systems involving fixed flowrate(non-mass-transfer-based)operations,based on the fictitious operations that is introduced to represent the water losing and/or generating operations and a modified concentration interval analysis(MCIA) technique.This strategy is a simple,nongraphical,and noniterative procedure and is suitable for the quick yields of targets and the identification of pinch point location.Moreover,on the basis of the target method,a heuristic-based approach is also presented to generate water utilization networks,which could be demonstrated to be optimum ones. The proposed approaches are illustrated with example problems.

Research on Distribution Regularities of Dust Concentration and Granularity in Large Mining Height Working Face

The key for dust control of coal mine is to clarify the dust concentration distribution and sedimentation in different areas. Both similarity experiment and numerical simulation method have certain restrictions and are quite different from the actual situation on site. In order to study the dust sedimentation regularity of coal mine in large mining height, “filter membrane method” is adopted in this paper, i.e., to dry and weigh the filter membrane before and after sampling, collect the dust of respirable zone on mining face and calculate the dust concentration based on a main airway of 100 m. The result shows that: A large amount of dust will be produced during coal mining, wherein the maximum dust concentration from 6 m upstream to 100 m downstream of coal cutter is 121 mg/m3, while the minimum dust concentration is 61 mg/m3;The dust concentration in return airway is reduced with the distance increases, while the dust concentration at the entrance is 91 mg/m3;A large amount of dust may fall from roof during section advancing and improves the dust concentration of hydraulic support in walking area obviously;The dust granularity of mining face and return airway is 0 - 100 μm, but the amount of respirable dust is higher than 80%, the larger the dust particle size, the higher the dust concentration. Besides, dust in small particle size can be suspended in air flow for longer, but that in large particle size may subside under the action of gravity;To reduce dust exposure, the mining position shall be located in the windward direction of advancing or coal cutter. This research can provide guidance for taking dust prevention measures of working face in large mining height.

Rapid Estimation on the Sperm Concentration in Boar Semen by Spectrophotometric Method

[ Objective] To establish the standardized spectrophotometric method to determine boar sperm concentration. [ Method ] The relation- ships between absorbance （A）, transmittance （T） and sperm concentration （C） of different wavelengths （450, 550, 650 nm） were compared. [ Result] The maximum sperm concentration detected by absorbance presented an upward trend with the increase of the wavelengths, 202 mitliorVml （450 nm）, 224 million/ml （550 nm） and 235 mUlion/ml （650 nm）, respectively, but the stability of repeated measurement was decreased. With the increase of sperm dilution times, the stability of repeated measurement of transmittance was reduced, and when dilution times were more than 10 times （450 nm）, 6 times （550 nm） and 4 times （650 nm）, differences appeared between the observed values of repeated measurement. [ Con- clusion] Wavelength at 450 nm was found to be the most sensitive and reliable, and sperm concentration presented cubic functional regression rela- tionship or power functional regression relationship with absorbance or transmittance, respectively. The regression equation for the standard curve at 450 nm was C400 = 0.48A3 - 0.76A2 ＋ 0.67A - 0.066 （ R = 0.951 ） and C400 = 1.657T -0.108. 8 （ R = 0.940）.

The Study of Area-Concentration Fractal Method in Litho-Geochemical Data in Tanurjeh Area, Khorasan Province

Given the scientific progresses as well as the invention of new methods in exploration, it is necessary to conduct some re-investigations in several exploration zones. So, in the present research, geochemical data on Tanurjeh exploration zone, (located in Northern Neishaboor, Khorasane Razavi province) is studied by using some modern statistical methods. Fractal methods are appropriated to study and separate the grades societies in deposits. In this article, litho-geochemical analysis results (ICP) are processed by concentration area fractal method (CA). The distribution diagrams related to the statistical populations are drawn, and anomaly populations of Copper, Gold and Molybdenum are determined besides previous studies (petrography and alteration), the results of statistic methods (CA) and aid presence of the porphyry system in depth.

Applicability of Gravity Separation Method on the Ashashire Gold Ore Deposit from Benishangul Gumuz Region, Western Ethiopia

The study was conducted to determine the applicability of gravity separation method on the Ashashire gold ore deposit Benishangul gumuz region, western Ethiopia. The Ashashire composite was produced to provide sufficient mass for this study and experiment, including sample preparation, mineralogical analysis of gold and associated elements, gravity concentration, and data interpretation and analysis. During the study, a grind optimization was conducted on the composites sample with varying grind size to evaluate the effect of grind size on gold recovery. The ore was moderately ground to the standard grind size of 80%, passing 106 µm, 75 µm, 53 µm and this nominal size was selected for the preliminary assessment for concentration optimization for this deposit. The gravity testing comprised three-stage concentration using Knelson concentrator. High recovery of gold from the gravity concentrates was achieved from the second gravity concentration. Based on the laboratory experimental result analysis, a grind size of P80 75 µm is selected as optimal size for the Ashashire gold deposit. Increasing the grind size from P80 of 75 µm to 106 µm decreases the recovery rate from 75% to 54%, or decreasing the grind size from P80 of 75 µm to 53 µm decreases the gold recovery rate to 37%. The native gold grain in the ores is mostly associated with quartz and fine gold is closely associated with pyrite. According to analysis of the fire assay, chemical, and mineralogical data, only gold and telluride is commercially valuable elements in the ores. Predominantly gold was occurred in the native form of Au-Te. The sample subjected to gravity separation assayed about 2.6 g/t Au.

Synthesize of ZnO Nanorods on Quartz Substrates with Seed Layer via Hydrothermal Method: Influence of Solution Concentration

Vertically oriented ZnO nanorods have been synthesized by hydrothermal method on quartz substrates with a seed layer. The influence of solution concentration on the morphology, structural and optical properties was analyzed. Results indicated that with the increase of solution concentration, the diameter and uniformity of ZnO nanorods increased. And the preferred orientation is obviously which shows better crystal quality. Typically, when the solution concentration is 0.03 mol/L, the nanorods exhibit a stronger UV emission peak located around 380 nm. In the visible region, all synthesized samples demonstrate more than 80% of optical transparency.

Multi-Scale Variation Prediction of PM2.5 Concentration Based on a Monte Carlo Method

Haze concentration prediction,especially PM2.5,has always been a significant focus of air quality research,which is necessary to start a deep study.Aimed at predicting the monthly average concentration of PM2.5 in Beijing,a novel method based on Monte Carlo model is conducted.In order to fully exploit the value of PM2.5 data,we take logarithmic processing of the original PM2.5 data and propose two different scales of the daily concentration and the daily chain development speed of PM2.5 respectively.The results show that these data are both approximately normal distribution.On the basis of the results,a Monte Carlo method can be applied to establish a probability model of normal distribution based on two different variables and random sampling numbers can also be generated by computer.Through a large number of simulation experiments,the average monthly concentration of PM2.5 in Beijing and the general trend of PM2.5 can be obtained.By comparing the errors between the real data and the predicted data,the Monte Carlo method is reliable in predicting the PM2.5 monthly mean concentration in the area.This study also provides a feasible method that may be applied in other studies to predict other pollutants with large scale time series data.

SCATTERING OF FLEXURAL WAVES AND DYNAMIC STRESS CONCENTRATIONS IN MINDLIN'S THICK PLATES WITH A CUTOUT

Using the complex variable method and conformal mapping,scat- tering of flexural waves and dynamic stress concentrations in Mindlin's thick plates with a cutout have been studied.The general solution of the stress problem of the thick plate satisfying the boundary conditions on the contour of cutouts is obtained. Applying the orthogonal function expansion technique,the dynamic stress problem can be reduced into the solution of a set of infinite algebraic equations.As examples, numerical results for the dynamic stress concentration factor in Mindlin's plates with a circular,elliptic cutout are graphically presented in sequence.

Effect of Yb^(3+) concentration on the structures and upconversion luminescence properties of Y_2O_3:Er^(3+) ultrafine phosphors

Y2O3：Er^3＋ ultrafine phosphors with a varying Yb^3＋ ion concentration were prepared by a urea homogeneous precipitation method. The results of XRD show that all the samples are of a pure cubic structure and the average crystallite sizes can be calculated as 45, 34, and 28 nm for Y2O3：Er^3＋ ultrafine phosphors with Yb^3＋ ion concentrations of 0, 10%, and 20%, respectively. The lattice constant and cell volume of the ultrafine phosphors decrease with enhancing Yb^3＋ ion concentration. The upconversion luminescence spectra of all the samples were studied under 980 nm laser excitation. The strong green and red upconversion emission were observed, and attributed to the ^2H11/2→^4I15/2, ^4S3/2 → ^4I15/2 and ^4F9/2 →^4I15/2 transitions of Er^3＋, respectively. The intensity of red emission increases with increasing Yb^3＋ ion concentration. The effect of Yb^3＋ ion concentration on the structures and upconversion luminescence mechanism were discussed.

MULTIPLICITY AND CONCENTRATION BEHAVIOUR OF POSITIVE SOLUTIONS FOR SCHRDINGER-KIRCHHOFF TYPE EQUATIONS INVOLVING THE p-LAPLACIAN IN R^N

In this article, we study the multiplicity and concentration behavior of positive solutions for the p-Laplacian equation of SchrSdinger-Kirchhoff type -εpM（εp-N∫RN｜△u｜p）△pu＋v（x｜u｜p-2u=f（u）in RN, where △p is the p-Laplacian operator, 1 〈 p 〈 N, M ： R＋ → R＋ and V ： RN →R＋ are continuous functions, ε is a positive parameter, and f is a continuous function with subcritical growth. We assume that V satisfies the local condition introduced by M. del Pino and P. Felmer. By the variational methods, penalization techniques, and Lyusternik- Schnirelmann theory, we prove the existence, multiplicity, and concentration of solutions for the above equation.

Particle characteristics and rheological constitutive relations of high concentration red mud

Red mud has relatively small solid particles （d50= 13.02 μm） and will flow in paste form under high pressure during pipeline transport. Red mud belongs to a two-phase flow of materials with high viscosity and a high concentration of non-sedimentation, homogeneous solid-liquids. It is difficult to test its rheological properties under atmospheric pressure. Measurements such as rotational viscometry can not reflect the real state of the material when it is flowing in a pipe. Tested rheological parameters are somewhat higher than the actual values. In our investigation, grain shape, distinctive modality and grain size distribution of red mud were tested. Based on the principle of tube measurement, rheological experiments on red mud at different concentrations were carried out by using our independently developed tube-type pressure theology test facility, and obtained constitutive equations. We conclude that red mud behaves as non-Newtonian pseudo-plastic fluid in pipe flows. Its consistency and power-law indices vary considerably with different concentrations.

Effect of Surface Topography on Stress Concentration Factor

Neuber rule and Arola-Ramulu model are widely used to predict the stress concentration factor of rough specimens. However, the height parameters and effective valley radius used in these two models depend strongly on the resolution of the roughness-measuring instruments and are easily introduce measuring errors. Besides, it is difficult to find a suitable parameter to characterize surface topography to quantitatively describe its effect on stress concentration factor. In order to overcome these disadvantages, profile moments are carried out to characterize surface topography, surface topography is simulated by superposing series of cosine components, the stress concentration factors of different micro cosine-shaped surface topographies are investigated by finite element analysis. In terms of micro cosine-shaped surface topography, an equation using the second profile moment to estimate the stress concentration factor is proposed, predictions for the stress concentration factor using the proposed expression are within 10% error compared with the results of finite element analysis, which are more accurate than other models. Moreover, the proposed equation is applied to the real surface topography machined by turning. Predictions for the stress concentration factor using the proposed expression are within 10% of the maximum stress concentration factors and about 5% of the effective stress concentration factors estimated from the finite element analysis for three levels of turning surface topographies under different simulated scales. The proposed model is feasible in predicting the stress concentration factors of real machined surface topographies.

AN ANALYSIS FOR DIFFRACTION OF FLEXURAL WAVES AND DYNAMIC STRESS CONCENTRATIONS IN THICK PLATES WITH A CAVITY

By using the complex variable method and conformal mapping, the diffraction of flexural waves and dynamic stress concentrations in thick plates with a cavity have been studied. A general solution of the stress problem of the thick plate satisfying the boundary conditions on the contour of an arbitrary cavity is obtained. By employing the orthogonal function expansion technique, the dynamic stress problem can be reduced to the solution of an infinite algebraic equation series. As an example, the numerical results for the dynamic stress concentration factor in thick plates with a circular, elliptic cavity are graphically presented. The numerical results are discussed.

DYNAMIC STRESS CONCENTRATIONS IN AMBARTSUMIAN'S PLATE WITH A CUTOUT

Based on the motion equations of flexural wave in Ambartsumian' s plates including the effects of transverse shear deformations,by using perturbation method of small parameter,the scatter- ing of flexural waves and dynamic stress concentrations in the plate with a cutout have been studied. The asypmtotic solution of the dynamic stress problem is obtained.Numerical results for the dynamic stress concentration factor in Ambartsumian's plates with a circular cutout are graphically presented and discussed.

Stress concentration factor expression for tension strip with eccentric elliptical hole

Two explicit expressions of the stress concentration factor for a tension finite-width strip with a central elliptical hole and an eccentric elliptical hole, respectively, are formulated by using a semi-analytical and semi-empiricai method. Accuracy of the results obtained from these expressions is better, and application scope is wider, than the results of Durelli＇s photo-elastic experiment and Isida＇s formula. When eccentricity of the elliptical hole is within a certain range, the error is less than 8%. Based on the relation between the stress concentration factor and the stress intensity factor, a stress intensity factor expression for tension strips with a center or an eccentric crack is derived with the obtained stress concentration factor expressions. Compared with the existing formulae and the finite element analysis, this stress intensity factor expression also has sufficient accuracy.

Influence of geometric parameters on stress concentration factors of undermatched butt joint with single V-groove under three-point bending load

In order to improve the bending load-carrying capacity （BLCC） of undermatched butt joint under three-point bending load, the influence of joint geometric parameters on stress concentration factors （SCF） at the weld bottom center and the weld toe of uudermatched butt joint with single V-groove are studied respectively based on the finite element method in this paper. Results show that the reinforcement height and the cover pass width play decisive role in the BLCC for undermatched butt joint. BLCC of undermatched butt joint can be improved by choosing the appropriate joint geometric parameters.

Existence and Concentration of Solutions for An Indefinite Schrodinger-Kirchhoff System

This paper is concerned with the nonlinear Schrodinger-Kirchhoff system-(a+b∫R^3/u/^2dx)△u+λV(x)u=f(x,u)in R^3,where constants a>0,6≥0 andλ>0 is a parameter.We require that V(x)∈C(R^3)and has a potential well V^-1(0).Combining this with other suitable assumptions on K and f,the existence of nontrivial solutions is obtained via variational methods.Furthermore,the concentration behavior of the nontrivial solution is also explored on the set V^-1(0)asλ→+∞as A—>H-oo as well.It is worth noting that the(PS)-condition can not be directly got as done in the literature,which makes the problem more complicated.To overcome this difficulty,we adopt different method.

Comparison of inversion of Chlorophyll concentration in the Bohai Bay based on GOCI Data

Bohai bay has poor water circulation capacity and low self-purification capacity,and a large number of land-sourced pollutants are accepted every year.The estimation of chlorophyll concentration based on remote sensing plays an important role in the study of marine red tide and the monitoring of water quality in the Bohai Bay.So it is necessary to select the best algorithm by comparing the performance of each algorithm.Choosing the best algorithm is helpful to improve the precision of chlorophyll concentration.Based on the in-situ measurement data of the Bohai Bay and the GOCI remote sensing reflectance data,the retrieval models of chlorophyll concentration were established in this paper by using the methods of fluorescence line height(FLH),OC3,blue-green band ratio and NIR-red band ratio.The above algorithms were evaluated by comparing the simulation results and the verification results.The results showed that there are obvious differences in the accuracy of different retrieval methods.The fitting and retrieval effect of FLH is the best and NIR-red band ratio is the worst,and the OC3is similar to the blue-green band ratio method.

An Exact Analytical Method for Magnetic Field Computation and Electromagnetic Torque in a Concentric Magnetic Gear

准确计算磁力齿轮电磁转矩是设计、分析磁力齿轮的关键,采用二维全局解析法计算同心式磁力齿轮气隙磁场。求解场域划分为内外转子永磁体、内外两层气隙和调磁定子的槽形区域,3类子区域的拉普拉斯方程和泊松方程通过边界连续条件建立联系。得到内外两层气隙区域的矢量磁位磁通密度解析表达式,有利于方便、快速、精确地计算任意转子位置的电磁转矩。计算了内外两层气隙磁场和内外转子电磁转矩,将气隙磁场波形和内外转子电磁转矩波形分别与二维有限元法计算波形作比较,结果吻合,证明了方法的正确性和有效性。