Laws move China a step further down market March 16 witnessed the approval of the landmark Property Law at the annual session of the National People’s Congress (NPC). This is the first time that equal protection ...Laws move China a step further down market March 16 witnessed the approval of the landmark Property Law at the annual session of the National People’s Congress (NPC). This is the first time that equal protection to state and private proper- ties展开更多
Objective: To explore the law and role of apoptosis inducing effect of allicin on human acute T-lymphocyte leukemia cell line (6T-CEM).Methods: Terminal deoxylnucleotide transferase directed X-UTP nick and end labelin...Objective: To explore the law and role of apoptosis inducing effect of allicin on human acute T-lymphocyte leukemia cell line (6T-CEM).Methods: Terminal deoxylnucleotide transferase directed X-UTP nick and end labeling (TUNEL) assay, DNA gel electrophoresis, light microscopy and revers transcription-polymerase chair reaction (RT-PCR) were used to observe apoptosis of cells.Results: Allicin showed significant inducing effect on apoptosis of 6T-CEM cell when the concentration was 0.1 μg/ml, reaching its peak effect when the concentration was 50 μg/ml, with the apoptosis rate as 36.4%. Allicin (15 μg/ml) acted on 6T-CEM for 2 h and induced obvious cell apoptosis, the highest effect reached after 24 h of action, with the apoptosis rate as 56.9%. Actidione or actinomycin D could induce apoptosis of 6T-CEM, the allicin could not enhance their apoptosis inducing effect. Allicin could cause significant reduction of bcl-2 and bax gene expression of 6T-CEM, especially the bcl-2.Conclusion: Allicin could induce cell apoptosis of 6T-CEM with significant time and dose effect. Mechanism of the effect is through inhibiting the bcl-2 gene expression to reduce bcl-2/bax ratio.展开更多
文摘Laws move China a step further down market March 16 witnessed the approval of the landmark Property Law at the annual session of the National People’s Congress (NPC). This is the first time that equal protection to state and private proper- ties
文摘Objective: To explore the law and role of apoptosis inducing effect of allicin on human acute T-lymphocyte leukemia cell line (6T-CEM).Methods: Terminal deoxylnucleotide transferase directed X-UTP nick and end labeling (TUNEL) assay, DNA gel electrophoresis, light microscopy and revers transcription-polymerase chair reaction (RT-PCR) were used to observe apoptosis of cells.Results: Allicin showed significant inducing effect on apoptosis of 6T-CEM cell when the concentration was 0.1 μg/ml, reaching its peak effect when the concentration was 50 μg/ml, with the apoptosis rate as 36.4%. Allicin (15 μg/ml) acted on 6T-CEM for 2 h and induced obvious cell apoptosis, the highest effect reached after 24 h of action, with the apoptosis rate as 56.9%. Actidione or actinomycin D could induce apoptosis of 6T-CEM, the allicin could not enhance their apoptosis inducing effect. Allicin could cause significant reduction of bcl-2 and bax gene expression of 6T-CEM, especially the bcl-2.Conclusion: Allicin could induce cell apoptosis of 6T-CEM with significant time and dose effect. Mechanism of the effect is through inhibiting the bcl-2 gene expression to reduce bcl-2/bax ratio.
