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Distributed Real-Time Systems on Responsive Link
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作者 Hiroyuki Chishiro Nobuyuki Yamassaki 《通讯和计算机(中英文版)》 2012年第12期1321-1330,共10页
关键词 分布式实时系统 链接 实时操作系统 应用程序接口 通信标准 API IEC 实时性
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End-to-End Utilization Control for Aperiodic Tasks in Distributed Real-Time Systems 被引量:2
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作者 廖勇 陈旭东 +2 位作者 熊光泽 朱清新 桑楠 《Journal of Computer Science & Technology》 SCIE EI CSCD 2007年第1期135-146,共12页
An increasing number of DRTS (Distributed model. The key challenges of such DRTS are guaranteeing Real-Time Systems) are employing an end-to-end aperiodic task utilization on multiple processors to achieve overload ... An increasing number of DRTS (Distributed model. The key challenges of such DRTS are guaranteeing Real-Time Systems) are employing an end-to-end aperiodic task utilization on multiple processors to achieve overload protection, and meeting the end-to-end deadlines of aperiodic tasks. This paper proposes an end-to-end utilization control architecture and an IC-EAT (Integration Control for End-to-End Aperiodic Tasks) algorithm, which features a distributed feedback loop that dynamically enforces the desired utilization bound on multiple processors. IC-EAT integrates admission control with feedback control, which is able to dynamically determine the QoS (Quality of Service) of incoming tasks and guarantee the end-to-end deadlines of admitted tasks. Then an LQOCM (Linear Quadratic Optimal Control Model) is presented. Finally, experiments demonstrate that, for the end-to-end DRTS whose control matrix G falls into the stable region, the IC-EAT is convergent and stable. Moreover,it is capable of providing better QoS guarantees for end-to-end aperiodic tasks and improving the system throughput. 展开更多
关键词 real-time scheduling end-to-end distributed real-time system feedback control scheduling aperiodic task
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Working Condition Real-Time Monitoring Model of Lithium Ion Batteries Based on Distributed Parameter System and Single Particle Model
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作者 黄亮 姚畅 《Chinese Journal of Chemical Physics》 SCIE CAS CSCD 2016年第5期623-628,I0002,共7页
Lithium ion batteries are complicated distributed parameter systems that can be described preferably by partial differential equations and a field theory. To reduce the solution difficulty and the calculation amount, ... Lithium ion batteries are complicated distributed parameter systems that can be described preferably by partial differential equations and a field theory. To reduce the solution difficulty and the calculation amount, if a distributed parameter system is described by ordinary differential equations (ODE) during the analysis and the design of distributed parameter system, the reliability of the system description will be reduced, and the systemic errors will be introduced. Studies on working condition real-time monitoring can improve the security because the rechargeable LIBs are widely used in many electronic systems and electromechanical equipment. Single particle model (SPM) is the simplification of LIB under some approximations, and can estimate the working parameters of a LIB at the faster simulation speed. A LIB modelling algorithm based on PDEs and SPM is proposed to monitor the working condition of LIBs in real time. Although the lithium ion concentration is an unmeasurable distributed parameter in the anode of LIB, the working condition monitoring model can track the real time lithium ion concentration in the anode of LIB, and calculate the residual which is the difference between the ideal data and the measured data. A fault alarm can be triggered when the residual is beyond the preset threshold. A simulation example verifies that the effectiveness and the accuracy of the working condition real-time monitoring model of LIB based on PDEs and SPM. 展开更多
关键词 Lithium ion battery distributed parameter system Single particle model Condition monitoring
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Scheduling transactions in mobile distributed real-time database systems 被引量:1
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作者 雷向东 赵跃龙 +1 位作者 陈松乔 袁晓莉 《Journal of Central South University of Technology》 EI 2008年第4期545-551,共7页
A DMVOCC-MVDA (distributed multiversion optimistic concurrency control with multiversion dynamic adjustment) protocol was presented to process mobile distributed real-time transaction in mobile broadcast environment... A DMVOCC-MVDA (distributed multiversion optimistic concurrency control with multiversion dynamic adjustment) protocol was presented to process mobile distributed real-time transaction in mobile broadcast environments. At the mobile hosts, all transactions perform local pre-validation. The local pre-validation process is carried out against the committed transactions at the server in the last broadcast cycle. Transactions that survive in local pre-validation must be submitted to the server for local final validation. The new protocol eliminates conflicts between mobile read-only and mobile update transactions, and resolves data conflicts flexibly by using multiversion dynamic adjustment of serialization order to avoid unnecessary restarts of transactions. Mobile read-only transactions can be committed with no-blocking, and respond time of mobile read-only transactions is greatly shortened. The tolerance of mobile transactions of disconnections from the broadcast channel is increased. In global validation mobile distributed transactions have to do check to ensure distributed serializability in all participants. The simulation results show that the new concurrency control protocol proposed offers better performance than other protocols in terms of miss rate, restart rate, commit rate. Under high work load (think time is ls) the miss rate of DMVOCC-MVDA is only 14.6%, is significantly lower than that of other protocols. The restart rate of DMVOCC-MVDA is only 32.3%, showing that DMVOCC-MVDA can effectively reduce the restart rate of mobile transactions. And the commit rate of DMVOCC-MVDA is up to 61.2%, which is obviously higher than that of other protocols. 展开更多
关键词 mobile distributed real-time database systems muliversion optimistic concurrency control multiversion dynamic adjustment pre-validation multiversion data broadcast
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Improvement Design for Distributed Real-Time Stream Processing Systems 被引量:4
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作者 Wei Jiang Liu-Gen Xu +1 位作者 Hai-Bo Hu Yue Ma 《Journal of Electronic Science and Technology》 CAS CSCD 2019年第1期3-12,共10页
In the era of Big Data, typical architecture of distributed real-time stream processing systems is the combination of Flume, Kafka, and Storm. As a kind of distributed message system, Kafka has the characteristics of ... In the era of Big Data, typical architecture of distributed real-time stream processing systems is the combination of Flume, Kafka, and Storm. As a kind of distributed message system, Kafka has the characteristics of horizontal scalability and high throughput, which is manly deployed in many areas in order to address the problem of speed mismatch between message producers and consumers. When using Kafka, we need to quickly receive data sent by producers. In addition, we need to send data to consumers quickly. Therefore, the performance of Kafka is of critical importance to the performance of the whole stream processing system. In this paper, we propose the improved design of real-time stream processing systems, and focus on improving the Kafka's data loading process.We use Kafka cat to transfer data from the source to Kafka topic directly, which can reduce the network transmission. We also utilize the memory file system to accelerate the process of data loading, which can address the bottleneck and performance problems caused by disk I/O. Extensive experiments are conducted to evaluate the performance, which show the superiority of our improved design. 展开更多
关键词 Kafka Kafka CAT memory FILE SYSTEM MESSAGE QUEUE real-time STREAM processing SYSTEM
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Developing Environment of Hierarchical and Distributed Real-Time Fire-Control Network 被引量:1
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作者 He Jianhua(Northwestern Polytechnical University, Xi’an 710072, P. R. China)(This project was supported by the Astronautics Foundation.) 《Journal of Systems Engineering and Electronics》 SCIE EI CSCD 1998年第4期1-7,共7页
This paper introduces a hierarchical real-time environment for developing ship-bornefire-control system. Advanced computer networks are used to simulate the system with the requiredengagement scenario, including own-s... This paper introduces a hierarchical real-time environment for developing ship-bornefire-control system. Advanced computer networks are used to simulate the system with the requiredengagement scenario, including own-ship and parameters, and data processing and transmission,mission calculation, graphical supervision and gunnery ballistics outputting. The simulation systemis able to receive instruction from, or send information to the command-control center. Furthermore,the system can also be used to compare various designed schemes and analyze the accuracy andeffectiveness of the system. 展开更多
关键词 distributed network Fire-control RS-485 communication Software environment
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A dynamic crash recovery scheme for distributed real-time database systems
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作者 肖迎元 刘云生 +2 位作者 刘小峰 廖国琼 王洪亚 《Journal of Shanghai University(English Edition)》 CAS 2006年第6期510-516,共7页
Recovery performance in the event of failures is very important for distributed real-time database systems. This paper presents a time-cognizant logging-based crash recovery scheme (TCLCRS) that aims at distributed ... Recovery performance in the event of failures is very important for distributed real-time database systems. This paper presents a time-cognizant logging-based crash recovery scheme (TCLCRS) that aims at distributed real-time databases, which adopts a main memory database as its ground support. In our scheme, each site maintains a real-time log for local transactions and the subtransactions, which execute at the site, and execte local checkpointing independently. Log records are stored in non-volatile high- speed store, which is divided into four different partitions based on transaction classes. During restart recovery after a site crash, partitioned crash recovery strategy is adopted to ensure that the site can be brought up before the entire local secondary database is reloaded in main memory. The partitioned crash recovery strategy not only guarantees the internal consistency to be recovered, but also guarantee the temporal consistency and recovery of the sates of physical world influenced by uncommitted transactions. Combined with two- phase commit protocol, TCLCRS can guarantee failure atomicity of distributed real-time transactions. 展开更多
关键词 distributed real-time database system partitioned real-time logging partitioned crash recovery.
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Quasi Serializable Concurrency Control in Distributed Real-Time Database Systems
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作者 党德鹏 Liu Yunsheng 《High Technology Letters》 EI CAS 2003年第1期72-76,共5页
This paper formally defines and analyses the new notion of correctness called quasi serializability, and then outlines corresponding concurrency control protocol QDHP for distributed real-time databases. Finally, thro... This paper formally defines and analyses the new notion of correctness called quasi serializability, and then outlines corresponding concurrency control protocol QDHP for distributed real-time databases. Finally, through a series of simulation studies, it shows that using the new concurrency control protocol the performance of distributed real-time databases can be much improved. 展开更多
关键词 distributed real-time database systems relaxing serializability real-tirne concurrency control
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GF-3 data real-time processing method based on multi-satellite distributed data processing system 被引量:5
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作者 YANG Jun CAO Yan-dong +2 位作者 SUN Guang-cai XING Meng-dao GUO Liang 《Journal of Central South University》 SCIE EI CAS CSCD 2020年第3期842-852,共11页
Due to the limited scenes that synthetic aperture radar(SAR)satellites can detect,the full-track utilization rate is not high.Because of the computing and storage limitation of one satellite,it is difficult to process... Due to the limited scenes that synthetic aperture radar(SAR)satellites can detect,the full-track utilization rate is not high.Because of the computing and storage limitation of one satellite,it is difficult to process large amounts of data of spaceborne synthetic aperture radars.It is proposed to use a new method of networked satellite data processing for improving the efficiency of data processing.A multi-satellite distributed SAR real-time processing method based on Chirp Scaling(CS)imaging algorithm is studied in this paper,and a distributed data processing system is built with field programmable gate array(FPGA)chips as the kernel.Different from the traditional CS algorithm processing,the system divides data processing into three stages.The computing tasks are reasonably allocated to different data processing units(i.e.,satellites)in each stage.The method effectively saves computing and storage resources of satellites,improves the utilization rate of a single satellite,and shortens the data processing time.Gaofen-3(GF-3)satellite SAR raw data is processed by the system,with the performance of the method verified. 展开更多
关键词 synthetic aperture radar full-track utilization rate distributed data processing CS imaging algorithm field programmable gate array Gaofen-3
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A multi-sensor-based distributed real-time measurement system for glacier deformation
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作者 DONG Han-chuan LIU Shuang +4 位作者 PANG Li-li TAO Zhi-gang FANG Li-de ZHANG Zhong-hua LI Xiao-ting 《Journal of Mountain Science》 SCIE CSCD 2023年第10期2913-2927,共15页
Glacier disasters occur frequently in alpine regions around the world,but the current conventional geological disaster measurement technology cannot be directly used for glacier disaster measurement.Hence,in this stud... Glacier disasters occur frequently in alpine regions around the world,but the current conventional geological disaster measurement technology cannot be directly used for glacier disaster measurement.Hence,in this study,a distributed multi-sensor measurement system for glacier deformation was established by integrating piezoelectric sensing,coded sensing,attitude sensing technology and wireless communication technology.The traditional Modbus protocol was optimized to solve the problem of data identification confusion of different acquisition nodes.Through indoor wireless transmission,adaptive performance analysis,error measurement experiment and landslide simulation experiment,the performance of the measurement system was analyzed and evaluated.Using unmanned aerial vehicle technology,the reliability and effectiveness of the measurement system were verified on the site of Galongla glacier in southeastern Tibet,China.The results show that the mean absolute percentage errors were only 1.13%and 2.09%for the displacement and temperature,respectively.The distributed glacier deformation real-time measurement system provides a new means for the assessment of the development process of glacier disasters and disaster prevention and mitigation. 展开更多
关键词 Glacier disasters distributed deformation measurement MULTI-SENSOR real-time LoRa Data adaptive.
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Transmission Considerations with QoS Support to Deliver Real-Time Distributed Speech Recognition Applications
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作者 Zhu Xiao-gang Zhu Hong-wen Rong Meng-tian 《Wuhan University Journal of Natural Sciences》 EI CAS 2002年第1期65-70,共6页
Distributed speech recognition (DSR) applications have certain QoS (Quality of service) requirements in terms of latency, packet loss rate, etc. To deliver quality guaranteed DSR application over wirelined or wireless... Distributed speech recognition (DSR) applications have certain QoS (Quality of service) requirements in terms of latency, packet loss rate, etc. To deliver quality guaranteed DSR application over wirelined or wireless links, some QoS mechanisms should be provided. We put forward a RTP/RSVP transmission scheme with DSR-specific payload and QoS parameters by modifying the present WAP protocol stack. The simulation result shows that this scheme will provide adequate network bandwidth to keep the real-time transport of DSR data over either wirelined or wireless channels. 展开更多
关键词 distributed speech recognition quality of service real-time transmission protocol resource reservation protocol wireless application protocol
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Assigning Real-Time Tasks in Environmentally Powered Distributed Systems
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作者 Jian Lin Albert M. K. Cheng 《Circuits and Systems》 2014年第4期98-113,共16页
Harvesting energy for execution from the environment (e.g., solar, wind energy) has recently emerged as a feasible solution for low-cost and low-power distributed systems. When real-time responsiveness of a given appl... Harvesting energy for execution from the environment (e.g., solar, wind energy) has recently emerged as a feasible solution for low-cost and low-power distributed systems. When real-time responsiveness of a given application has to be guaranteed, the recharge rate of obtaining energy inevitably affects the task scheduling. This paper extends our previous works in?[1] [2] to explore the real-time task assignment problem on an energy-harvesting distributed system. The solution using Ant Colony Optimization (ACO) and several significant improvements are presented. Simulations compare the performance of the approaches, which demonstrate the solutions effectiveness and efficiency. 展开更多
关键词 distributed Systems Energy HARVESTING real-time SCHEDULING TASK ASSIGNMENT
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Weak Serializable Concurrency Control in Distributed Real-Time Database Systems
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作者 党德鹏 刘云生 潘琳 《Journal of Shanghai University(English Edition)》 CAS 2002年第4期325-330,共6页
Most of the proposed concurrency control protocols for real time database systems are based on serializability theorem. Owing to the unique characteristics of real time database applications and the importance of sa... Most of the proposed concurrency control protocols for real time database systems are based on serializability theorem. Owing to the unique characteristics of real time database applications and the importance of satisfying the timing constraints of transactions, serializability is too strong as a correctness criterion and not suitable for real time databases in most cases. On the other hand, relaxed serializability including epsilon serializability and similarity serializability can allow more real time transactions to satisfy their timing constraints, but database consistency may be sacrificed to some extent. We thus propose the use of weak serializability(WSR) that is more relaxed than conflicting serializability while database consistency is maintained. In this paper, we first formally define the new notion of correctness called weak serializability. After the necessary and sufficient conditions for weak serializability are shown, corresponding concurrency control protocol WDHP(weak serializable distributed high priority protocol) is outlined for distributed real time databases, where a new lock mode called mask lock mode is proposed for simplifying the condition of global consistency. Finally, through a series of simulation studies, it is shown that using the new concurrency control protocol the performance of distributed real time databases can be greatly improved. 展开更多
关键词 distributed real time database systems relaxed serializability real time concurrency control read only transactions.
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A Cooperative Distributed System for Real-Time Route Guidance
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作者 Yaser E. Hawas 《Journal of Transportation Technologies》 2012年第3期230-240,共11页
This paper describes a cooperative decentralized architecture for reactive real-time route guidance. The architecture is cooperative in the sense that it allows adjacent local controllers to exchange information regar... This paper describes a cooperative decentralized architecture for reactive real-time route guidance. The architecture is cooperative in the sense that it allows adjacent local controllers to exchange information regarding the traffic conditions in their territories. A set of local decision rules and associated heuristic functions to support the cooperative architecture are specified. A protocol governing the knowledge exchange among local adjacent controllers is developed. A simulation-assignment modeling framework is used for assessing the effectiveness of this cooperative architecture under various levels of controller knowledge and network traffic congestion. The cooperative decentralized system is tested under various scenarios of knowledge and cooperation and network traffic demand levels. The cooperative system is compared against the shortest path algorithm as a benchmark. 展开更多
关键词 DECENTRALIZED System real-time ROUTE GUIDANCE LOCAL Controllers COOPERATIVE Systems Micro-Scopic Simulation
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Research on Collaboration Theory of Distributed Measurement System and Real-Time of Communication Platform
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作者 SHENYan 《Journal of Electronic Science and Technology of China》 2005年第1期95-95,共1页
关键词 distributed measurement system agent technology swarm intellgence Particle Swarm Optimization algorithm Collaboration model Switched Ethernet real-time Scheduling AEROENGINE
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Real-Time Traffic State and Boundary Flux Estimation with Distributed Speed Detecting Networks
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作者 Yichi Zhang Heng Deng 《Journal of Transportation Technologies》 2022年第4期533-543,共11页
The rapid development of 5G mobile communication and portable traffic detection technologies enhances highway transportation systems in detail and at a vehicle level. Besides the advantage of no disturbance to the reg... The rapid development of 5G mobile communication and portable traffic detection technologies enhances highway transportation systems in detail and at a vehicle level. Besides the advantage of no disturbance to the regular traffic operation, these ubiquitous sensing technologies have the potential for unprecedented data collection at any temporal and spatial position. While as a typical distributed parameter system, the freeway traffic dynamics are determined by the current system states and the boundary traffic demand-supply. Using the three-step extended Kalman filtering, this paper simultaneously estimates the real-time traffic state and the boundary flux of freeway traffic with the distributed speed detector networks organized at any location of interest. In order to assess the effectiveness of the proposed approach, a freeway segment from Interstate 80 East (I-80E) in Alameda, Emeryville, and Northern California is selected. Experimental results show that the proposed method has the potential of using only speed detecting data to monitor the state of urban freeway transportation systems without access to the traditional measurement data, such as the boundary flows. 展开更多
关键词 Traffic State Boundary Flux Estimation Extended Kalman Filtering distributed Speed Detecting Networks
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基于Real-time PCR法检测乳粉中牛源性成分定量研究
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作者 陈晨 史国华 +5 位作者 陈勃旭 张瑞 王玉欣 贾文珅 陈佳 周巍 《粮油食品科技》 CAS CSCD 北大核心 2024年第2期159-164,共6页
基于Real-timePCR建立了乳粉中牛源性成分相对定量检测方法,并对牛的特异性引物与探针进行了特异性、灵敏度和稳定性测试。通过模拟不同浓度牛乳粉与马乳粉混合样本,根据其△Ct值的函数关系进行线性拟合进而绘制标准曲线,建立乳粉中牛... 基于Real-timePCR建立了乳粉中牛源性成分相对定量检测方法,并对牛的特异性引物与探针进行了特异性、灵敏度和稳定性测试。通过模拟不同浓度牛乳粉与马乳粉混合样本,根据其△Ct值的函数关系进行线性拟合进而绘制标准曲线,建立乳粉中牛源性成分的相对定量检测。结果显示,该方法的最低检测限为0.00001 mg/mL,回收率为91.11%~119.2%,组间变异系数≤0.58%、组内变异系数≤1.44%。说明该方法在特异性与稳定性上适用于乳粉中牛源性成分及含量的掺假检测。 展开更多
关键词 牛乳粉 马乳粉 real-time PCR 掺假检测
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A multifunctional shear apparatus for rocks subjected to true triaxial stress and high temperature in real-time 被引量:1
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作者 Jun Zhao Xia-Ting Feng +2 位作者 Jia-Rong Wang Liang Hu Yue Guo 《Journal of Rock Mechanics and Geotechnical Engineering》 SCIE CSCD 2024年第9期3524-3543,共20页
Deep engineering disasters,such as rockbursts and collapses,are more related to the shear slip of rock joints.A novel multifunctional device was developed to study the shear failure mechanism in rocks.Using this devic... Deep engineering disasters,such as rockbursts and collapses,are more related to the shear slip of rock joints.A novel multifunctional device was developed to study the shear failure mechanism in rocks.Using this device,the complete shearedeformation process and long-term shear creep tests could be performed on rocks under constant normal stiffness(CNS)or constant normal loading(CNL)conditions in real-time at high temperature and true-triaxial stress.During the research and development process,five key technologies were successfully broken through:(1)the ability to perform true-triaxial compressioneshear loading tests on rock samples with high stiffness;(2)a shear box with ultra-low friction throughout the entire stress space of the rock sample during loading;(3)a control system capable of maintaining high stress for a long time and responding rapidly to the brittle fracture of a rock sample as well;(4)a refined ability to measure the volumetric deformation of rock samples subjected to true triaxial shearing;and(5)a heating system capable of maintaining uniform heating of the rock sample over a long time.By developing these technologies,loading under high true triaxial stress conditions was realized.The apparatus has a maximum normal stiffness of 1000 GPa/m and a maximum operating temperature of 300C.The differences in the surface temperature of the sample are constant to within5C.Five types of true triaxial shear tests were conducted on homogeneous sandstone to verify that the apparatus has good performance and reliability.The results show that temperature,lateral stress,normal stress and time influence the shear deformation,failure mode and strength of the sandstone.The novel apparatus can be reliably used to conduct true-triaxial shear tests on rocks subjected to high temperatures and stress. 展开更多
关键词 True-triaxial shear apparatus ROCKS Complete shear stress-deformation process CREEP real-time high-temperature
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Distributed Nash Equilibrium Seeking Strategies Under Quantized Communication 被引量:2
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作者 Maojiao Ye Qing-Long Han +2 位作者 Lei Ding Shengyuan Xu Guobiao Jia 《IEEE/CAA Journal of Automatica Sinica》 SCIE EI CSCD 2024年第1期103-112,共10页
This paper is concerned with distributed Nash equi librium seeking strategies under quantized communication. In the proposed seeking strategy, a projection operator is synthesized with a gradient search method to achi... This paper is concerned with distributed Nash equi librium seeking strategies under quantized communication. In the proposed seeking strategy, a projection operator is synthesized with a gradient search method to achieve the optimization o players' objective functions while restricting their actions within required non-empty, convex and compact domains. In addition, a leader-following consensus protocol, in which quantized informa tion flows are utilized, is employed for information sharing among players. More specifically, logarithmic quantizers and uniform quantizers are investigated under both undirected and connected communication graphs and strongly connected digraphs, respec tively. Through Lyapunov stability analysis, it is shown that play ers' actions can be steered to a neighborhood of the Nash equilib rium with logarithmic and uniform quantizers, and the quanti fied convergence error depends on the parameter of the quan tizer for both undirected and directed cases. A numerical exam ple is given to verify the theoretical results. 展开更多
关键词 CONSENSUS distributed Nash equilibrium seeking projected gradient play quantized communication
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一种基于real-time PCR技术的TTV检测方法的建立及应用
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作者 贾毅博 王高玉 +4 位作者 邓宛心 林彩云 杨华 陈运春 尹飞飞 《海南医学院学报》 CAS 北大核心 2024年第7期489-497,共9页
目的:本研究旨在开发一种具有更高灵敏度和特异性的TTV检测技术,为揭示TTV在多种疾病过程中的作用提供重要的技术支持。方法:为了更精确、灵敏的检测TTV,本研究分析了目前公布的所有亚型的TTV基因序列,在此基础上建立了一种基于UTR区域... 目的:本研究旨在开发一种具有更高灵敏度和特异性的TTV检测技术,为揭示TTV在多种疾病过程中的作用提供重要的技术支持。方法:为了更精确、灵敏的检测TTV,本研究分析了目前公布的所有亚型的TTV基因序列,在此基础上建立了一种基于UTR区域的real-time PCR检测方法,并与文献报道应用较为广泛的PCR检测方法进行了对比。结果:本研究建立的方法在1×10^(7)~1×10^(1) copies/μL标准品浓度范围内具有良好的线性关系,相关系数为1.000,斜率为-3.446,检测下限为1×10^(1) copies/μL。重复性试验结果显示,组内变异系数为7.22%,表明本方法重复性、稳定性较强。针对30份临床样本,使用本研究建立的real-time PCR检测方法及目前被多个研究所使用的4套引物进行对比。结果表明,本研究所建立的方法灵敏度显著高于文献中报道的4种方法(P<0.01);Sanger测序结果表明,本方法检测出的30份阳性样本均为TTV,检测特异性为100%。结论:本研究采用基于TaqMan探针的real-time PCR检测方法,检测灵敏性高、覆盖基因型范围广,尤其对于TTV病毒载量较低的情况下能够进行定量检测,对于TTV病毒的致病性及作为免疫标志物的应用提供重要的技术支持。 展开更多
关键词 Torque teno virus 基因组扩增测序 real-time PCR检测
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