Pyrrolidine dithiocarbamate alleviates the anti-tuberculosis drug-induced liver injury through JAK2/STAT3 signaling pathway:An experimental study

Objective:To study the effect of pyrrolidine dithiocarbamate(PDTC) on the anti-tuberculosis drug-induced liver injury and the molecular mechanism. Methods:Clean male SD rats were selected as experimental animals and randomly divided into normal group,model group,PDTC group and AG490 group. Animal model of anti-tuberculosis drug-induced liver injury was established by intragastric administration isoniazid + rifampicin. PDTC group received intraperitoneal injection of PDTC,and AG490 group received intraperitoneal injection of AG490. Twenty-eight days after intervention,the rats were executed,and the liver injury indexes,inflammation indexes and oxidative stress indexes in serum as well as JAK2/STAT3 expression,liver injury indexes,inflammation indexes and oxidative stress indexes in liver tissue were determined. Results:p-JAK2,p-STAT3,TNF-α,IL-1β,IL-6,ROS,8-OHdG and MDA expression in liver tissue as well as TBIL,ALT,AST,γ-GT,TNF-α,IL-1β,IL-6,ROS,8-OHdG and MDA levels in serum of model group were significantly higher than those of normal group while p-JAK2,p-STAT3,TNF-α,IL-1β,IL-6,ROS,8-OHdG and MDA expression in liver tissu as well as TBIL,ALT,AST,γ-GT,TNF-α,IL-1β,IL-6,ROS,8-OHdG and MDA levels in serum of PDTC group and AG490 group were significantly lower than those of model group. Conclusions:PDTC can inhibit the inflammation and oxidative stress mediated by JAK2/STAT3 signaling pathway to alleviate the anti-tuberculosis drug-induced liver injury.

Protective effect of pyrrolidine dithiocarbamate on liver injury induced by intestinal ischemia-reperfusion in rats

BACKGROUND: The nuclear translocation of transcription factors may be a critical factor in the intracellular pathway involved in ischemia/reperfusion(I/R) injury. The aim of the study was to evaluate the role of nuclear factor-kappa B (NF-κB) in the pathogenesis of liver injury induced by intestinal ischemia/reperfusion (IIR) and to investigate the effect of pyrrolidine dithiocarbamate (PDTC) on this liver injury. METHODS: Male Wistar rats were divided randomly into three experimental groups (8 rats in each): sham operation group (control group); intestinal/reperfusion group(I/R group): animals received 1-hour of intestinal ischemia and 2-hour reperfusion; and PDTC treatment group (PDTC group): animals that received I/R subject to PDTC treatment (100 mg/kg). The histological changes in the liver and intestine were observed, and the serum levels of tumor necrosis factor-α (TNF-α), alanine aminotransferase (ALT), aspartate aminotransferase (AST), liver superoxide dismutase (SOD), and nitrite/nitrate (NO) were measured. The immunohistochemical expression and Western blot analysis of liver NF-κB and intercellular adhesion molecule-1(ICAM-1) were observed. RESULTS: IIR induced liver injury characterized by the histological changes of liver edema, hemorrhage, polymorphonuclear neutrophil (PMN) infiltration, and elevated serum levels of AST and ALT. The serum TNF-α level was significantly higher than that of the control group(P<0.01) and a high level of liver oxidant product was observed (P<0.01). These changes were parallel to the positive expression of NF-κB and ICAM-1. After the administration of PDTC, the histological changes after liver injury were improved; the levels of SOD and NO in the liver were elevated and reduced, respectively (P<0.01). The expressions of ICAM-1 and NF-κB in the liver were weakened (P<0.01). CONCLUSION: NF-κB plays an important role in the pathogenesis of liver injury induced by HR. PDTC, an agent known to inhibit the activation of NF-κB, can reduce and prevent this injury.

Pyrrolidine dithiocarbamate and saxagliptin ameliorate ulcerative colitis in rats

Objective: To evaluate the antioxidant, immunomodulatory and anti-inflammatory activities of pyrrolidine dithiocarbamate and saxagliptin in rats with thioacetamide-induced ulcerative colitis. Methods: Animals were orally administered with a vehicle, sulfasalazine(500 mg/kg), pyrrolidine dithiocarbamate(100 mg/kg), and saxagliptin(10 mg/kg) for two weeks. Ulcerative colitis was induced by a single intrarectal instillation of thioacetamide on day 8. Colon samples were collected to assess mitogen-activated protein kinase(MAPK), phosphorylated extracellular signal-regulated kinase(ERK), c AMP response element-binding protein(CREB), interleukin-12(IL-12), caspase-3, β-defensin, inducible nitric oxide synthase(i NOS) and glucagon like peptide-1(GLP-1). Moreover, histopathological examination was performed. Results: Rats treated with thioacetamide caused increases in colonic MAPK, phosphorylated ERK, CREB, caspase-3, IL-12, β-defensin, i NOS, as well as decreases in body weight and GLP-1. In addition, distortion of colonic structure was found by histopathological examination. Pyrrolidine dithiocarbamate and saxagliptin mitigated colitis severity by improving body weight decrease and GLP-1, and reducing colonic MAPK, phosphorylated ERK, CREB, caspase-3, IL-12, β-defensin and i NOS. Conclusions: Pyrrolidine dithiocarbamate and saxagliptin are efficient against thioacetamide induced colitis through improving inflammatory and oxidative changes.

Activation of nuclear factor-kappa B and effects of pyrrolidine dithiocarbamate on TNBS-induced rat colitis

AIM: To explore the changes of nuclear factor-kappa B (NF-κB) DNA-binding activity, the expression of intercellular adhesion molecule-1 (ICAM-1) regulated by NF-κB at various times and to evaluate the effects of pyrrolidine dithiocarbamate (PDTC) on trinitrobenzene sulfonic acid (TNBS)-induced rat colitis.METHODS: TNBS of 0.6 mL was mixed with ethanol of 0.3 mL solution and instilled into the lumen of the rat colon. The rat models were divided into 6 groups, which were killed at 24 h, 3, 7, 14, and 21 d after enema. Colonic inflammation and damage were assessed by macroscopical and histological criteria. Activity of NF-κB DNA-binding was analyzed by electrophoresis mobility shift assays (EMSA).Expression of ICAM-1 was detected by in situ hybridization (ISH) and immunohistochemistry (IH). Then various doses of PDTC were injected into rat abdomen 30 min before enema with TNBS/ethanol as pretreatment. The rats were killed 4 h after enema and the colonic inflammation,myeloperoxidase (MPO) activity, malondialdehyde (MDA)level, and DNA-binding activity of NF-κB were assessed.Finally, PDTC was injected intraperitoneally after colitis was induced. Changes of morphology were assayed.RESULTS: During the first week, hyperemia, hemorrhage,edema and ulceration of the colonic mucosa appeared with predominant infiltration of leukocytes. Neutrophils,macrophages, lymphocytes infiltrated in mucosa and submucosa 14 d later. Fibroblasts and granuloma-like structures were also obviously seen. The binding activity of NF-κB began to increase at 24 h time point and reached a peak at 14 d, then decreased but still was higher than control group at 21 d (P＜0.01). Levels of tCAM-1 mRNA and protein significantly elevated at 24 h and the peak was at 21 d. Pretreatment with PDTC could attenuate the development of inflammation but not by reducing NF-κB activity. This attenuation of inflammation had a positive relationship with the dose of PDTC. PDTC at the dose of 100 mg/kg had no therapeutic effect after colitis was induced.CONCLUSION: NF-κB activation is an important event that may be involved in acute and chronic inflammation development and may contribute to self-protection against early inflammation damage. NF-κB also regulates ICAM-1expression during colonic inflammation. Pretreatment of PDTC may attenuate the inflammation development. But PDTC has no therapeutic effect after the colitis is induced.

Evaluation of the effect of pyrrolidine dithiocarbamate in suppressing inflammation in mice with dextran sodium sulfate-induced colitis

AIM: To evaluate the effect of pyrrolidine dithio- carbamate (PDTC; an NF-κB inhibitor) administered at low (50 mg/kg) and high (100 mg/kg) doses in suppressing colitis in mice with dextran sodium sulfate (DSS)-induced colitis. METHODS: Mice were divided into a DSS-untreated group (normal group), DSS-treated control group, DSS+PDTC-treated groupⅠ(low-dose group), and DSS+PDTC-treated groupⅡ (high-dose group). In each group, the disease activity index score (DAI score), intestinal length, histological score, and the levels of activated NF-κB and inflammatory cytokines (IL-1β and TNF-α) in tissue were measured. RESULTS: The DSS+PDTC-treated groupⅡ exhibited suppression of shortening of intestinal length and reduction of DAI score. Activated NF-κB level and IL-1β and TNF-α levels were significantly lower in DSS+PDTC- treated groupⅡ. CONCLUSION: These findings suggest that PDTC is useful for the treatment of ulcerative colitis.

Pyrrolidine Dithiocarbamate (PDTC) Attenuates Luteolin-Induced Apoptosis in Human Leukemia HL-60 Cells

Studies have indicated that flavonoid luteolin is a potential inhibitor of tumor cell proliferation and may function as an anticarcinogenic agent. Pyrrolidine dithiocarbamate (PDTC), a synthetic compound, may exhibit biphasic effects on apoptosis depending on the experimental context. Previously, we found that luteolin induced the activation of the proapoptotic proteins, such as Bad, Bid, and Bax, in HL-60 human leukemia cells. We also explored the modulatory effects and molecular mechanisms of PDTC on the cytotoxicity of luteolin in HL-60 cells;PDTC could interfere with luteolin’s ability to cleave poly(ADP-ribose)-polymerase (PARP) and DNA fragmentation of factor-45 (DFF-45). In the current study, we further investigated the effect of PDTC on the luteolin-induced death-receptor pathway and the cleavage of the Bcl-2 family members. We found that the combination of luteolin and PDTC increased the survival of the HL-60 cells such that PDTC inhibited both extrinsic and intrinsic pathways in luteolin-induced apoptosis.

Synthesis and Structure of a Ruthenium(Ⅱ)-dithiocarbamate Complex [RuH(CO)(PPh_3)_2(4-ClPhNHCS_2)]·C_6H_(14)

The title complex [RuH（CO）（PPh3）2（4-ClPhNHCS2）]·C6H14 has been prepared and characterized by X-ray diffraction analysis.It crystallizes in the triclinic system,space group P1 with a = 11.0817（2）,b = 14.3889（2）,c = 15.2136（2） ,α = 71.018（1）,β = 74.911（1）,γ = 85.146（1）°,V = 2214.86（6） 3,Z = 2,Mr = 900.4,Dc = 1.350 g/cm3,Mr = 900.40,μ（MoKα） = 0.616 mm-1,F（000） = 926,S = 1.016,the final R = 0.0478 and wR = 0.0947 for 6828 observed reflections with I 2σ（I） and 505 variables.The molecular structure of 1 consists of one neutral complex [RuH（CO）（PPh3）2（4-ClPhNHCS2）] and one hexane solvent molecule.The geometry around ruthenium is pseudo-octahedral with two trans-binding PPh3 ligands and one chelating bidentate 4-ClPhNHCS2- ligand via two sulfur atoms.The average Ru-S,Ru-P and Ru-H bond lengths are 2.4824（8）,2.3495（8） and 1.71（2）,respectively.The electrochemical properties of 1 have been studied in CH2Cl2 solution by cyclic voltammetry.

Syntheses,Characterization and Crystal Structures of Dithiocarbamate-based Mononuclear Palladium(Ⅱ) Complexes

By employing the dithiocarbamate salt （K（PPDC）, where PPDC = 4？-pyridyl-1-pipe-razine-4-dithiocarbamate） as the functional ligand and di-palladium complexes [（N^N）2Pd2（NO3ˉ）2]（NO3ˉ）2 （N^N = 2,2＇-bipyridine, bpy; 4,4＇-dimethylbipyridine, dmbpy） as corner, two novel single metal complexes with Pd（II） centers have been obtained. These organic-metal complexes were characterized by NMR, ESI-MS, elemental analysis, Uv-vis spectra and single-crystal X-ray diffraction analysis. Compound 1？（PF6）2 （[（bpy）Pd（PPDC）]·（PF6）2） crystallizes in triclinic, space group P , a = 8.3968（5）, b = 11.5565（7）, c = 18.2234（11）, α = 97.505（1）, β = 91.424（1）, γ = 106.146（1）o, C22H24N6S2P2F12Pd, Mr = 832.93, V = 1680.58（18） ？3, Z = 2, Dc = 1.646 Mg/m3, μ（MoKα） = 0.863 mm-1, F（000） = 828, the final R = 0.0455 and wR = 0.1390 for 6981 observed reflections with I 〉 2σ（I）. Similarly, compound 2？（PF6）2 （[（dmbpy）Pd（PPDC）]·（PF6）2） also crystallizes in triclinic, space group P , a = 13.9467（3）, b = 14.8390（2）, c = 17.0632（3） ？, α = 81.8680（10）, β = 87.051（2）, γ = 83.4590（10）o, C22H25N5S2P2F12Pd, Mr = 819.93, V = 3470.81（11） ？3, Z = 4, Dc = 1.569 Mg/m3, μ（CuKα） = 7.115 mm-1, F（000） = 1632, the final R = 0.0606 and wR = 0.1637 for 12835 observed reflections with I 〉 2σ（I）. Crystallography reveals that each metal center coordinates with two N atoms from bpy and two S atoms from PPDC in the square coordination mode. In the crystal structure of complex 1, a weak Pd……Pd interaction can be observed. Interestingly, it was also found that the mononuclear moieties of complex 2 could be packed into a 3-D porous framework via multiple intermolecular C–F……H hydrogen-boding interactions which extended in the a, b, and c axes with PF6ˉ anions frozen inside.

Synthesis, Crystal Structure and Antibacterial Activity of Dibenzyl Dithiocarbamate Bismuth Complex

A new complex, {Bi(Ⅲ)[S2CN(CH2C6H5)2]3}2·2CH3CN, was synthesized and characterized by elemental analysis, IR, TG, 1H NMR and X-ray single-crystal diffraction. The complex crystallizes in the triclinic system, space group P1, with a = 12.705(6), b = 13.430(6), c = 15.489(12) , α = 102.135(12), β = 99.649(12), γ = 111.521(9)°, Z = 1, V = 2315(2) A3, Dc = 1.531 g·cm-3, F(000) = 1068, μ = 4.116 mm-1, the final R = 0.0559, w R = 0.1069 and S =1.056. The coordinated geometry of Bi(Ⅲ) with six sulfur atoms from three ligands is a distorted pentagonal pyramidal configuration, and the structural system is formed by weak interactions of Bi···S between two molecules. The complex was valued for its antibacterial activity by agar-streak method. It was found that the complex is weakly active against three of the five tested bacterial organisms.

Synthesis and Crystal Structure of a Molybdenum Carbonyl Compound with Thiolate and Dithiocarbamate Ligands, [Bu_4N][(OC)_4Mo(μ-SC_6H_5)_2Mo(C_5H_(10)CNS_2)(CO)_2]

A di-molybdenum carbonyl compound containing thiolate and dithiocarbamate ligands, [Bu4N][（CO）4Mo（μ-SC6H5）2Mo（C5H10dtc）（CO）2] 1 （CsH10dtc = S2CNC5H10）, has been prepared by reaction of [Mo2（SC6H5）2（CO）8] with C5H10dtcNa and [NBu4]Br in acetone. It crystallizes in monoclinic, space group P21/n with a = 13.162（3）, b = 17.466（2）, c = 20.453（4） A,β= 100.77（1）°, Z = 4, V= 4619（2）A^3, C40H56Mo2N2O6S4, Mr = 980.95, De= 1.389 g/cm^3,μ = 7.66 cm^-1, F（000） = 1988 and R = 0.0746 for 5161 observed reflections with I 〉 2σ（I）. The complex contains a [Mo2S2]^2- planar core in which one Mo atom is chelated by a C5H10dtc ligand, leading to different coordination environments of the two Mo atoms. 95Mo NMR measurement indicates that the two Mo atoms are in different oxidation states.

Syntheses, Crystal Structures and Anticancer Activities of Dithiocarbamate Bismuth(Ⅲ) Complexes

Two novel complexes, [Bi（III）（S2CNBn Et）3] 1 and [Bi（III）（S2CNPPPy-2）3] 2, were synthesized and characterized by elemental analysis, IR, ^1H NMR, ^13C NMR, TG-DTG and X-ray single-crystal diffraction. The structure reveals that complex 1 belongs to the monoclinic system, space group P21/c with a = 11.457（5）, b = 17.437（8）, c = 33.439（14）, β= 98.499（8）o, Z = 4, V = 6607（5）3, Dc = 1.689 g·cm^-3, F（000） = 3328, μ= 5.742 mm^-1, the final R = 0.0906, w R = 0.1678 and S = 1.032. The structure indicates that complex 2 belongs to the triclinic system, space group P1 with a = 12.608（9）, b = 12.622（8）, c = 12.49（6）, α = 102.652（15）, β = 103.013（14）, γ = 109.698（10）o, Z = 1, V = 1762.3（19）3, Dc = 1.741 g·cm^-3, F（000） = 916, μ = 5.395 mm^-1, the final R = 0.048 2, w R = 0.1206 and S =1.046. In the two complexes, each six-coordinated Bi（III） atom adopts a pentagonal pyramidal configuration with six sulfur atoms from three ligands, and the dimmer structures are formed by weak interactions of Bi···S between two molecules. The anticancer activities of the two complexes were studied by evaluating their cytotoxicities against several human cancer cell lines using the MTT assay. Results indicated complex 2 showed moderate cytotoxic effects on the selected cancer cells.

Molybdenum Dithiocarbamates:A New Approach to "Old" Technology

A new method for the preparation of dinuclear molybdenum dithiocarbamates(MoDDCs) is reported.This new method allows for the control of the amount of sulfurization of the Mo2SxO4-x core(x = 1 to 4) in the dinuclear MoDDCs.This control assists in the tailoring of specific tribological properties of the MoDDCs in both greases and lubricating oils.

A Facile and Efficient One-Pot Regioselective Synthesis of 2-Hydroxyalkyl Dithiocarbamates under Catalyst-Free Conditions

A simple catalyst-free, and highly regioselective approach to 2-hydroxyalkyl dithiocarbamates is described which involves a one-pot reaction of various amines and carbon disulfide,CS2, with epoxides in ethanol at room temperature.

Synthesis, Characterization and Antimicrobial Activities of Sulfadiazine Schiff Base and Phenyl Dithiocarbamate Mixed Ligand Metal Complexes

Sulfadiazine Schiff base and Phenyl dithiocarbamate mixed ligand metal complexes have been synthesized and characterized. The characterization of metal complexes is based on the results of the solubility, colour, melting points and elemental analysis as well as the spectroscopic studies. The results obtained from the spectroscopic spectra revealed from the UV-visible showed the presence of chromophoric groups while the FTIR results confirmed that the metals coordinated through the two nitrogen atoms of Sulfadiazine Schiff base and sulphur atom of the N-phenyl dithiocarbamate. Also the antimicrobial activities of the metal complexes proved that the metal complexes are more active than the parent ligands.

The Lead-Chelating Effects of Substituted Dithiocarbamates

The effectiveness of certain substituted dithiocarbamates in mobilizing lead from preexposed rats was investigated.The animals received 10mg Pb/kg/day, intragastrically.for 8 weeks and were treated thereafter with 400μmol/kg, intraperitoneally.of morpholine dithiocarbamate, tetraammonium ethylenediamine diacetic acid dithiocarbamate (EDDTC), ammonium diethanolamine dithiocarbamate (ADDTC), sodium diethyldithiocarbamate.N-benzyl-D-glucamine dithiocarbamate (NBGDTC), or dimercaptosuccinic acid, daily for 5 days.All the chelating agents were effective in lowering the hepatic and renal burden of Pb.ADDTC.EDDTC, and NBGDTC were also able to lower the long bone Pb content.The lowering of Pb burden had no relationship to restoration of Pb-induced hematopoietic alterations.The relatively lower lipophilicity of substituted dithiocarbamates, owing to the presence of hydrophilic groups, seems to be advantageous in preventing passage of metal chelale into the brain.None of the substituted dithiocarbamates caused excessive excretion of urinary Cu and Zn.ADDTC and EDDTC appear to be promising in the management of Pb poisoning. 1990 Academic Press.Inc.

A Device for Rapid Detection of Dithiocarbamate Pesticide Residues in Fruits

Based on the chemical properties of dithiocarbamate pesticides,a device for rapid detection was developed in the paper,and the experimental conditions were optimized. Dithiocarbamate residues in fruits were successfully detected using molecular absorption spectro-photometry,and the recovery rate was over 80%.The rapid detection method was simple to operate with low cost,and was conducive to application in basic level and enterprise laboratories.

Synthesis, Characterization and <i>In-Vitro</i>Antimicrobial Studies of M²⁺+ Complexes of <i>p</i>-Chlorophenyl-, <i>p</i>-Bromophenyl-Dithiocarbamates

This study reports the synthesis of metal complexes of mixed ligands p-Chlorophenyl-, p-Bromophenyl-dithiocarbamates. The spectral properties of the metal complexes [Zn(II), Cd(II), Hg(II), Cu(II), Co(II) and Ni(II)] were obtained using the FTIR and UV/Vis spectroscopic techniques. Their antibacterial studies revealed that these complexes as well as the ligands could serve as possible antibacterial agents against pathogens. Melting point and solubility measurements were also carried out. It was observed that these complexes were non-toxic, non-electrolytic and stable solids.

Investigation of the False Positive Results of Dithiocarbamate Pesticides due to Endogenous Nonpathogenic Carbon Disulfide in Organically Grown Plants (Moringa oleifera)

Moringa plant belongs to the family Moringaceae within the order Brassicales. It is a magical plant which contains 7 timesthe vitamin C content of oranges,4 times the vitamin A content of carrots, 4 times the calcium content of milk ,3 times the potassiumcontent of bananas and 2 times the protein content of yogurt (gram to gram) . The most widely distributed species is Moringa oleiferaLam., referred as drumstick tree for the shape of its seed pods. Leaves of M. oleifera are rich in a unique glucosinolate namedglucomoringin. Moringa were found to release CS2 when submitted to experimental conditions of dithiocarbamate residue analysis.Gas chromatography was used to quantitate CS2 and control samples were obtained from Moringa plantations cultivated inexperimental areas, in which no treatment with fungicides of the dithiocarbamate group was applied. Endogenous CS2 levels werecompared with dithiocarbamate residues measured in Moringa samples from the field trials following applications of the macozeebfungicide. Use small leaves resulted in an observed decrease in CS2 concentration comparable to the large leaves. Temperature levelused has an effect on the concentration of CS2 measured as an indicator for dithiocarbamate pesticide residue. And use of hightemperature (100 °C) in drying of Moringa leaves after harvesting may lead to some biochemical changes that caused an observedincrease in CS2 concentration comparable to drying at (50 °C). Evidence for formation of dithiocarbamate compound from moringinin moringa leaves are metabolized/detoxified principally by the mercapturic acid pathway. Conjugation with glutathione (GSH)promoted by glutathione transferases gives rise to the corresponding Glucosinolate -. Isothiocyanate conjugates. These undergofurther enzymatic modifications to give rise sequentially to the cysteinylglycine cysteine - Isothiocyanate andN-acetylcysteine-Isothiocyanate conjugates, all of which are dithiocarbamates. The detection of an ion with a m/z value 430.40, withthe following fragments m/z 165.10 and m/z 121.05 respectively, among the ions analyzed by GC-MS gives a clear evidence forformation of dithiocarbamate by addition of glutathione to the isothiocyanate formed from glucosinolate in moringa plant.

Synthesis and Properties of Rare Earth Complexes with 3-Ferrocenyl-5-Phenyl-Pyrazoline-1-Dithiocarbamate

Sodium 3-ferrocenyl-5-phenyl-pyrazoline-1-dithiocarbamate (FPPD) reacts with anhydrous rare earth chlorides in absolute ethanol to give 14 new complexes.The results of elemental analysis show that the compositions of the complexes are RE(FPPD)_3·nH_2O(RE=La,Pr～Lu and Y;n=3).The ligand and its complexes have been characterized by infrared spectroscopy,UV-visible absorption spectroscopy,~1H-NMR spectra,thermal analysis and molar conductivity measurement.

DTCR协同水泥固化/稳定化重金属污染底泥的研究

采用二硫代氨基甲酸盐(DTCR)为添加剂协同水泥固化/稳定化重金属污染底泥,以抗压强度和颗粒固化体(粒径9.5mm)浸出毒性为指标确定水泥和DTCR的最优配比.通过酸雨条件(pH 3)下对颗粒固化体和整个固化体的浸出试验来评价固化/稳定化的效果.利用X射线衍射仪(XRD)和环境扫描电镜(ESEM)分析了固化/稳定化机理.结果表明,固化/稳定化的最优配比为水泥掺入量为50%(干底泥),DTCR掺入量为2%(干底泥).其固化体7d抗压强度为1.03MPa,颗粒固化体中重金属Cu,Zn,Pb,Cd的浸出浓度分别为0.105,4.65,0.232,0.123mg/L,能够达到安全填埋要求.酸雨条件下(pH 3)对颗粒固化体和整个固化体浸出研究表明,水泥、DTCR固化/稳定化底泥效果更好;XRD和ESEM分析表明,固化/稳定化的机理主要是水泥在水化反应时,能够形成水化产物Ca(OH)2、水化硅酸钙(C-S-H)和钙矾石(AFt),将重金属废物包容,并逐步硬化形成具有一定强度的水泥固化体.