This article tries to find out the few discrepancies between the teachers and their scholars as to oral activity, which shows that scholars' expectations and needs are not quite responded to. The versions chosen as a...This article tries to find out the few discrepancies between the teachers and their scholars as to oral activity, which shows that scholars' expectations and needs are not quite responded to. The versions chosen as a sample for this study come from different schools in the academy of Rabat Sal~ Zemmour Zaer area and belong to three different educational levels including common core, first year, and second year in addition to a total number of 40 teachers who have contributed to this research throughout the whole process. This research has relied on different instruments to collect the data including questionnaires, interviews, and class observation grids. Results showed that scholars' perceptions highly favour thematic variety. Findings also reported match findings of previous research in highlighting the importance of using tech facilities and multimedia to improve oral activity.展开更多
DEAR EDITOR,Understanding the different immune responses of wild and domestic caprid species is critical for addressing certain zoonotic diseases.In this study,we generated blood transcriptomes of 13 Siberian ibex and...DEAR EDITOR,Understanding the different immune responses of wild and domestic caprid species is critical for addressing certain zoonotic diseases.In this study,we generated blood transcriptomes of 13 Siberian ibex and domestic goat hybrids and performed allele-specific expression and splicing analyses.Results showed that genes exhibiting significant imbalance between the ibex and goat were highly related to the Toll-like receptor(TLR),antigen recognition,and immune activation pathways.Comparative genomic analysis of the species revealed that immune function-related genes were under strong selection pressure in the domestic goat.展开更多
China is a hotspot of relict plant species that were once widespread throughout the Northern Hemisphere.Recent research has demonstrated that the occurrence of long-term stable refugia in the mountainous regions of ce...China is a hotspot of relict plant species that were once widespread throughout the Northern Hemisphere.Recent research has demonstrated that the occurrence of long-term stable refugia in the mountainous regions of central and south-western China allowed their persistence through the late Neogene climate fluctuations.One of these relict lineages is Dipteronia,an oligotypic tree genus with a fossil record extending to the Paleocene.Here,we investigated the genetic variability,demographic dynamics and diversification patterns of the two currently recognized Dipteronia species(Dipteronia sinensis and D.dyeriana).Molecular data were obtained from 45 populations of Dipteronia by genotyping three cpDNA regions,two single copy nuclear genes and 15 simple sequence repeat loci.The genetic study was combined with niche comparison analyses on the environmental space,ecological niche modeling,and landscape connectivity analysis.We found that the two Dipteronia species have highly diverged both in genetic and ecological terms.Despite the incipient speciation processes that can be observed in D.sinensis,the occurrence of long-term stable refugia and,particularly,a dispersal corridor along Daba Shan-west Qinling,likely ensured its genetic and ecological integrity to date.Our study will not only help us to understand how populations of Dipteronia species responded to the tectonic and climatic changes of the Cenozoic,but also provide insight into how Arcto-Tertiary relict plants in East Asia survived,evolved,and diversified.展开更多
We describe a new species of Discostella from Guiyang based on light and scanning electron microscopic observations,as well as molecular analysis.This species is morphologically distinguished from other species by a r...We describe a new species of Discostella from Guiyang based on light and scanning electron microscopic observations,as well as molecular analysis.This species is morphologically distinguished from other species by a relatively smooth and slightly recessed central area with marginal striae of unequal lengths forked near the margin.Marginal fultoportulae and a single rimoportula are situated between every two costae or on the costae.Both morphological characters and the concatenated data of rbc L and SSU rDNA indicate that Discostella guiyangensis is a unique and new species.According to divergence time estimation analysis,Discostella guiyangensis is the oldest species among the Discostella species sequenced currently.In addition,we complement molecular data of Discostella asterocostata and Discostella stelligera.展开更多
Transportation accessibility has been treated as an important means of reducing the urban-rural income disparity.However,only a few studies have examined the effects of different types of transportation accessibility ...Transportation accessibility has been treated as an important means of reducing the urban-rural income disparity.However,only a few studies have examined the effects of different types of transportation accessibility on urban-rural income disparity and their spatial heterogeneity.Based on data from 285 prefecture-level(and above)Chinese cities in 2000,2005,2010,2015,and 2020,this study uses spatial econometric models to examine how highway accessibility and railway accessibility influence the urban-rural income disparity and to identify their spatial heterogeneity.The result reveals that highway accessibility and railway accessibility have‘coreperiphery’ring-like circle structures.The urban-rural income disparity exhibits strong spatial clustering effects.Both highway accessibility and railway accessibility are negatively associated with urban-rural income disparity,and the former having a greater effect size.Moreover,there is a substitution effect between highway accessibility and railway accessibility in the whole sample.Furthermore,these associations differ in geographic regions.In the central region,highway accessibility is more important in reducing the urban-rural income disparity,but its effect is weakened with the increase of railway accessibility.In the western region,railway accessibility has a larger effect on narrowing the urban-rural income disparity,and this effect is strengthened by the increase of highway accessibility.We conclude that improving transportation accessibility is conducive to reducing the urban-rural income disparity but its effect is spatial heterogenetic.Highways and railways should be developed in a coordinated manner to promote an integrated transport network system.展开更多
We study equations in divergence form with piecewise Cαcoefficients.The domains contain corners and the discontinuity surfaces are attached to the edges of the corners.We obtain piecewise C^(1,α) estimates across th...We study equations in divergence form with piecewise Cαcoefficients.The domains contain corners and the discontinuity surfaces are attached to the edges of the corners.We obtain piecewise C^(1,α) estimates across the discontinuity surfaces and provide an example to illustrate the issue regarding the regularity at the corners.展开更多
Besides the rapid retreating trend of Arctic sea-ice extent(SIE),this study found the most outstanding low-frequency variation of SIE to be a 4-6-year periodic variation.Using a clustering analysis algorithm,the SIE i...Besides the rapid retreating trend of Arctic sea-ice extent(SIE),this study found the most outstanding low-frequency variation of SIE to be a 4-6-year periodic variation.Using a clustering analysis algorithm,the SIE in most ice-covered regions was clustered into two special regions:Region-1 around the Barents Sea and Region-2 around the Canadian Basin,which were located on either side of the Arctic Transpolar Drift.Clear 4-6-year periodic variation in these two regions was identified using a novel method called“running linear fitting algorithm”.The rate of temporal variation of the Arctic SIE was related to three driving factors:the regional air temperature,the sea-ice areal flux across the Arctic Transpolar Drift,and the divergence of sea-ice drift.The 4-6-year periodic variation was found to have always been present since 1979,but the SIE responded to different factors under heavy and light ice conditions divided by the year 2005.The joint contribution of the three factors to SIE variation exceeded 83%and 59%in the two regions,respectively,remarkably reflecting their dynamic mechanism.It is proven that the process of El Niño-Southern Oscillation(ENSO)is closely associated with the three factors,being the fundamental source of the 4-6-year periodic variations of Arctic SIE.展开更多
In anurans,advertisement calls(ACs)are an essential form of intraspecific communication.This study evaluates geographical variation in the ACs of Leptobrachella ventripunctata in the Guizhou Plateau,southwestern China...In anurans,advertisement calls(ACs)are an essential form of intraspecific communication.This study evaluates geographical variation in the ACs of Leptobrachella ventripunctata in the Guizhou Plateau,southwestern China,and explores correlations between call characteristics,body size,and environmental factors.ACs are simple calls of L.ventripunctata,and apparent differences were observed in the ACs among different geographical populations of L.ventripunctata.The Call duration(CD)revealed a significant positive correlation with altitude and a significant negative correlation with temperature and humidity.Moreover,the Dominant frequency(DF)exhibited a significant negative correlation with altitude and the habitat closure degree and a significant positive correlation with temperature.These variations in ACs between different geographical populations of L.ventripunctata may critically impact the adaptive evolution of species,and the calls may also be relevant for environmental selection.展开更多
Magnetic field design is essential for the operation of Hall thrusters.This study focuses on utilizing a genetic algorithm to optimize the magnetic field configuration of SPT70.A 2D hybrid PIC-DSMC and channel-wall er...Magnetic field design is essential for the operation of Hall thrusters.This study focuses on utilizing a genetic algorithm to optimize the magnetic field configuration of SPT70.A 2D hybrid PIC-DSMC and channel-wall erosion model are employed to analyze the plume divergence angle and wall erosion rate,while a Farady probe measurement and laser profilometry system are set up to verify the simulation results.The results demonstrate that the genetic algorithm contributes to reducing the divergence angle of the thruster plumes and alleviating the impact of high-energy particles on the discharge channel wall,reducing the erosion by 5.5%and 2.7%,respectively.Further analysis indicates that the change from a divergent magnetic field to a convergent magnetic field,combined with the upstream shift of the ionization region,contributes to the improving the operation of the Hall thruster.展开更多
The longitudinal dispersion of the projectile in shooting tests of two-dimensional trajectory corrections fused with fixed canards is extremely large that it sometimes exceeds the correction ability of the correction ...The longitudinal dispersion of the projectile in shooting tests of two-dimensional trajectory corrections fused with fixed canards is extremely large that it sometimes exceeds the correction ability of the correction fuse actuator.The impact point easily deviates from the target,and thus the correction result cannot be readily evaluated.However,the cost of shooting tests is considerably high to conduct many tests for data collection.To address this issue,this study proposes an aiming method for shooting tests based on small sample size.The proposed method uses the Bootstrap method to expand the test data;repeatedly iterates and corrects the position of the simulated theoretical impact points through an improved compatibility test method;and dynamically adjusts the weight of the prior distribution of simulation results based on Kullback-Leibler divergence,which to some extent avoids the real data being"submerged"by the simulation data and achieves the fusion Bayesian estimation of the dispersion center.The experimental results show that when the simulation accuracy is sufficiently high,the proposed method yields a smaller mean-square deviation in estimating the dispersion center and higher shooting accuracy than those of the three comparison methods,which is more conducive to reflecting the effect of the control algorithm and facilitating test personnel to iterate their proposed structures and algorithms.;in addition,this study provides a knowledge base for further comprehensive studies in the future.展开更多
A generalized labeled multi-Bernoulli(GLMB)filter with motion mode label based on the track-before-detect(TBD)strategy for maneuvering targets in sea clutter with heavy tail,in which the transitions of the mode of tar...A generalized labeled multi-Bernoulli(GLMB)filter with motion mode label based on the track-before-detect(TBD)strategy for maneuvering targets in sea clutter with heavy tail,in which the transitions of the mode of target motions are modeled by using jump Markovian system(JMS),is presented in this paper.The close-form solution is derived for sequential Monte Carlo implementation of the GLMB filter based on the TBD model.In update,we derive a tractable GLMB density,which preserves the cardinality distribution and first-order moment of the labeled multi-target distribution of interest as well as minimizes the Kullback-Leibler divergence(KLD),to enable the next recursive cycle.The relevant simulation results prove that the proposed multiple-model GLMB-TBD(MM-GLMB-TBD)algorithm based on K-distributed clutter model can improve the detecting and tracking performance in both estimation error and robustness compared with state-of-the-art algorithms for sea clutter background.Additionally,the simulations show that the proposed MM-GLMB-TBD algorithm can accurately output the multitarget trajectories with considerably less computational complexity compared with the adapted dynamic programming based TBD(DP-TBD)algorithm.Meanwhile,the simulation results also indicate that the proposed MM-GLMB-TBD filter slightly outperforms the JMS particle filter based TBD(JMSMeMBer-TBD)filter in estimation error with the basically same computational cost.Finally,the impact of the mismatches on the clutter model and clutter parameter is investigated for the performance of the MM-GLMB-TBD filter.展开更多
Integrated sensing and communication(ISAC)is regarded as a pivotal technology for 6G communication.In this paper,we employ Kullback-Leibler divergence(KLD)as the unified performance metric for ISAC systems and investi...Integrated sensing and communication(ISAC)is regarded as a pivotal technology for 6G communication.In this paper,we employ Kullback-Leibler divergence(KLD)as the unified performance metric for ISAC systems and investigate constellation and beamforming design in the presence of clutters.In particular,the constellation design problem is solved via the successive convex approximation(SCA)technique,and the optimal beamforming in terms of sensing KLD is proven to be equivalent to maximizing the signal-to-interference-plus-noise ratio(SINR)of echo signals.Numerical results demonstrate the tradeoff between sensing and communication performance under different parameter setups.Additionally,the beampattern generated by the proposed algorithm achieves significant clutter suppression and higher SINR of echo signals compared with the conventional scheme.展开更多
Aims Possible shifts in the phenotypic performance along invasive plants’spreading route are rarely examined due to the discontinuous and incomplete records of exotic species.As the invasion history of common ragweed...Aims Possible shifts in the phenotypic performance along invasive plants’spreading route are rarely examined due to the discontinuous and incomplete records of exotic species.As the invasion history of common ragweed(Ambrosia artemisiifolia L.)is well documented in Hungary,its residence time is known for each location.By sampling a sequence of older to more recently established populations,we aimed to determine(i)whether there are phenotypic divergences along the historical spreading route of A.artemisiifolia;(ii)which traits are under selection during the invasion process and(iii)the extent of maternal effects on the individual’s performance.Methods We used a hierarchical sampling design to collect seeds from 64 individuals belonging to eight sites in four residence time categories(seven populations along the historical spreading route of ragweed in Hungary and one recently invaded site in Romania).We selected four large and four small individual plants in each population to control for maternal effects.The offspring were reared in a common garden located in Romania.Five vegetative phenotypic traits were measured at the end of the experiments and used in the subsequent analysis(plant height,basal diameter,number of secondary axes,length of the longest secondary axis and biomass).To summarize the variation of these highly correlated traits,principal component analysis was performed first and then the important components were used in linear mixed effect models.Important Findings The residence time categories were significantly distinguished by the first component,which compresses the variation of all five measured traits.The measures gradually decrease from populations with the longest residence time(introduced more than 65 years ago)towards the most recently established populations(established less than 30 years ago).These differences might reflect the invasion history of the populations:the longer the residence time the higher the chance to develop relevant traits beneficial in invasion process.The size of the mother plant significantly influenced not only the seed mass(inversely)but also the adult performance of its offspring(directly).展开更多
The genus Rhododendron(Ericaceae),with more than 1000 species highly diverse in f lower color,is providing distinct ornamental values and a model system for f lower color studies.Here,we investigated the divergence be...The genus Rhododendron(Ericaceae),with more than 1000 species highly diverse in f lower color,is providing distinct ornamental values and a model system for f lower color studies.Here,we investigated the divergence between two parental species with different f lower color widely used for azalea breeding.Gapless genome assembly was generated for the yellow-f lowered azalea,Rhododendron molle.Comparative genomics found recent proliferation of long terminal repeat retrotransposons(LTR-RTs),especially Gypsy,has resulted in a 125 Mb(19%)genome size increase in species-specific regions,and a significant amount of dispersed gene duplicates(13402)and pseudogenes(17437).Metabolomic assessment revealed that yellow f lower coloration is attributed to the dynamic changes of carotenoids/f lavonols biosynthesis and chlorophyll degradation.Time-ordered gene co-expression networks(TO-GCNs)and the comparison confirmed the metabolome and uncovered the specific gene regulatory changes underpinning the distinct f lower pigmentation.B3 and ERF TFs were found dominating the gene regulation of carotenoids/f lavonols characterized pigmentation in R.molle,while WRKY,ERF,WD40,C2H2,and NAC TFs collectively regulated the anthocyanins characterized pigmentation in the red-f lowered R simsii.This study employed a multi-omics strategy in disentangling the complex divergence between two important azaleas and provided references for further functional genetics and molecular breeding.展开更多
Background The genomes of worldwide poultry breeds divergently selected for performance and other phenotypic traits may also be affected by,and formed due to,past and current admixture events.Adaptation to diverse env...Background The genomes of worldwide poultry breeds divergently selected for performance and other phenotypic traits may also be affected by,and formed due to,past and current admixture events.Adaptation to diverse environments,including acclimation to harsh climatic conditions,has also left selection footprints in breed genomes.Results Using the Chicken 50K_CobbCons SNP chip,we genotyped four divergently selected breeds:two aboriginal,cold tolerant Ushanka and Orloff Mille Fleur,one egg-type Russian White subjected to artificial selection for cold tolerance,and one meat-type White Cornish.Signals of selective sweeps were determined in the studied breeds using three methods:(1)assessment of runs of homozygosity islands,(2)F_(ST) based population differential analysis,and(3)haplotype differentiation analysis.Genomic regions of true selection signatures were identified by two or more methods or in two or more breeds.In these regions,we detected 540 prioritized candidate genes supplemented them with those that occurred in one breed using one statistic and were suggested in other studies.Amongst them,SOX5,ME3,ZNF536,WWP1,RIPK2,OSGIN2,DECR1,TPO,PPARGC1A,BDNF,MSTN,and beta-keratin genes can be especially mentioned as candidates for cold adaptation.Epigenetic factors may be involved in regulating some of these important genes(e.g.,TPO and BDNF).Conclusion Based on a genome-wide scan,our findings can help dissect the genetic architecture underlying various phenotypic traits in chicken breeds.These include genes representing the sine qua non for adaptation to harsh environments.Cold tolerance in acclimated chicken breeds may be developed following one of few specific gene expression mechanisms or more than one overlapping response known in cold-exposed individuals,and this warrants further investigation.展开更多
Cryptic species are commonly misidentified because of high morphological similarities to other species.One group of plants that may harbor large numbers of cryptic species is the quillworts(Isoetes spp.),an ancient aq...Cryptic species are commonly misidentified because of high morphological similarities to other species.One group of plants that may harbor large numbers of cryptic species is the quillworts(Isoetes spp.),an ancient aquatic plant lineage.Although over 350 species of Isoetes have been reported globally,only ten species have been recorded in China.The aim of this study is to better understand Isoetes species diversity in China.For this purpose,we systematically explored the phylogeny and evolution of Isoetes using complete chloroplast genome(plastome)data,spore morphology,chromosome number,genetic structure,and haplotypes of almost all Chinese Isoetes populations.We identified three ploidy levels of Isoetes in Chinaddiploid(2n=22),tetraploid(2n=44),and hexaploid(2n=66).We also found four megaspore and microspore ornamentation types in diploids,six in tetraploids,and three in hexaploids.Phylogenetic analyses confirmed that I.hypsophila as the ancestral group of the genus and revealed that Isoetes diploids,tetraploids,and hexaploids do not form monophyletic clades.Most individual species possess a single genetic structure;however,several samples have conflicting positions on the phylogenetic tree based on SNPs and the tree based on plastome data.All 36 samples shared 22 haplotypes.Divergence time analysis showed that I.hypsophila diverged in the early Eocene(~48.05 Ma),and most other Isoetes species diverged 3-20 Ma.Additionally,different species of Isoetes were found to inhabit different water systems and environments along the Yangtze River.These findings provide new insights into the relationships among Isoetes species in China,where highly similar morphologic populations may harbor many cryptic species.展开更多
The underlying causes of biodiversity disparities among geographic regions have long been a fundamental theme in ecology and evolution.However,the patterns of phylogenetic diversity(PD) and phylogenetic beta diversity...The underlying causes of biodiversity disparities among geographic regions have long been a fundamental theme in ecology and evolution.However,the patterns of phylogenetic diversity(PD) and phylogenetic beta diversity(PBD) of congeners that are disjunctly distributed between eastern Asia-eastern North America(EA-ENA disjuncts) and their associated factors remain unknown.Here we investigated the standardized effect size of PD(SES-PD),PBD,and potentially associated factors in 11natural mixed forest sites(five in EA and six in ENA) where abundant EA-ENA disjuncts occur.We found that the disjuncts in ENA possessed higher SES-PD than those in EA at the continental scale(1.96vs-1.12),even though the number of disjunct species in ENA is much lower than in EA(128 vs 263).SESPD of the EA-ENA disjuncts tended to decrease with increasing latitude in 11 sites.The latitudinal diversity gradient of SES-PD was stronger in EA sites than in ENA sites.Based on the unweighted unique fraction metric(UniFrac) distance and the phylogenetic community dissimilarity,PBD showed that the two northern sites in EA were more similar to the six-site ENA group than to the remaining southern EA sites.Based on the standardized effect size of mean pairwise distances(SES-MPD),nine of eleven studied sites showed a neutral community structure(-1.96 ≤SES-MPD ≤1.96).Both Pearson’s r and structural equation modeling suggested that SES-PD of the EA-ENA disjuncts was mostly associated with mean divergence time.Moreover,SES-PD of the EA-ENA disjuncts was positively correlated with temperaturerelated climatic factors,although negatively correlated with mean diversification rate and community structure.By applying approaches from phylogenetics and community ecology,our work sheds light on historical patterns of the EA-ENA disjunction and paves the way for further research.展开更多
The estimation of model parameters is an important subject in engineering.In this area of work,the prevailing approach is to estimate or calculate these as deterministic parameters.In this study,we consider the model ...The estimation of model parameters is an important subject in engineering.In this area of work,the prevailing approach is to estimate or calculate these as deterministic parameters.In this study,we consider the model parameters from the perspective of random variables and describe the general form of the parameter distribution inference problem.Under this framework,we propose an ensemble Bayesian method by introducing Bayesian inference and the Markov chain Monte Carlo(MCMC)method.Experiments on a finite cylindrical reactor and a 2D IAEA benchmark problem show that the proposed method converges quickly and can estimate parameters effectively,even for several correlated parameters simultaneously.Our experiments include cases of engineering software calls,demonstrating that the method can be applied to engineering,such as nuclear reactor engineering.展开更多
Leaf-mining flies(Diptera:Agromyzidae)are a diverse family of small-bodied insects that feed on living plant tissues as larvae.Various species in this family are considered globally invasive and have caused great agri...Leaf-mining flies(Diptera:Agromyzidae)are a diverse family of small-bodied insects that feed on living plant tissues as larvae.Various species in this family are considered globally invasive and have caused great agricultural economic losses.In China,economically important vegetable crops have been seriously damaged by these pest insects,especially by species of the genus Liriomyza.However,these species are difficult to differentiate because of their morphological similarities,and the Chinese fauna remains poorly known.To explore the relevant pest species in China and their phylogeny,agromyzid leafminers were collected from 2016 to 2019,and identified based on morphological characteristics and DNA barcodes.In total,27 species from five genera of Agromyzidae were sampled and identified,including 16species of Liriomyza.Both mitochondrial and nuclear genes were used to reconstruct their phylogenetic relationships and estimate the divergence time.Highly congruent and well-supported phylogenetic trees were obtained using the Bayesian inference and maximum-likelihood methods.This analysis revealed two main clades in Liriomyza,and clade 2was inferred to have diverged from clade 1 approximately 27.40 million years ago(95%highest posterior density:23.03–31.52 million years ago)in the Oligocene.Differences were observed in the distribution patterns and host associations between the Liriomyza clades.Clade 2 species are distributed in cool,high-latitude environments,suggesting that they may have evolved into a cool-adapted lineage.展开更多
Sorghum[Sorghum bicolor(L.)Moench],a multipurpose C4 crop,is also a model species of the Poaceae family for plant research.During the process of domestication,the modification of seed dispersal mode is considered a ke...Sorghum[Sorghum bicolor(L.)Moench],a multipurpose C4 crop,is also a model species of the Poaceae family for plant research.During the process of domestication,the modification of seed dispersal mode is considered a key event,as the loss of seed shattering caused a significant increase in yield.In order to understand the seed shattering process in sorghum,we further studied eight previously identified divergent sorghum germplasm with different shattering degrees.We described their phenotypes in great detail,analyzed the histology of abscission zone,and conducted a gene co-expression analysis.We observed that the abscission layer of the most strong-shattering varieties began to differentiate before the 5-10 cm panicles development stage and was completely formed at flag leaf unfolding.The protective cells on the pedicels were also fully lignified by flowering.Through the weighted gene correlation network analysis(WGCNA),we mined for candidate genes involved in the abscission process at the heading stage.We found that these genes were mainly associated with such biological processes as hormone signal transmission(SORBI_3003G361300,SORBI_3006G216500,SORBI_3009G027800,SORBI_3007G077200),cell wall modification and degradation(SORBI_3002G205500,SORBI_3004G013800,SORBI_3010G022400,SORBI_3003G251800,SORBI_3003G254700,SORBI_3003G410800,SORBI_3009G162700,SORBI_3001G406700,SORBI_3004G042700,SORBI_3004G244600,SORBI_3001G099100),and lignin synthesis(SORBI_3004G220700,SORBI_3004G062500,SORBI_3010G214900,SORBI_3009G181800).Our study has provided candidate genes required for shedding for further study.We believe that function characterization of these genes may provide insight into our understanding of seed shattering process.展开更多
文摘This article tries to find out the few discrepancies between the teachers and their scholars as to oral activity, which shows that scholars' expectations and needs are not quite responded to. The versions chosen as a sample for this study come from different schools in the academy of Rabat Sal~ Zemmour Zaer area and belong to three different educational levels including common core, first year, and second year in addition to a total number of 40 teachers who have contributed to this research throughout the whole process. This research has relied on different instruments to collect the data including questionnaires, interviews, and class observation grids. Results showed that scholars' perceptions highly favour thematic variety. Findings also reported match findings of previous research in highlighting the importance of using tech facilities and multimedia to improve oral activity.
基金supported by the National Natural Science Foundation of China(32002140,32050410304)China Agriculture Research System of MOF and MARA(CARS-39-21)+3 种基金Natural Science Basic Research Program of Shaanxi(2021JCW-11)Natural Science Basic Research Program of Xinjiang(2022 XJRMY-01)Special Fund for Animal Husbandry of Shaanxi(K3030821073)China Postdoctoral Science Foundation(2020M682446)。
文摘DEAR EDITOR,Understanding the different immune responses of wild and domestic caprid species is critical for addressing certain zoonotic diseases.In this study,we generated blood transcriptomes of 13 Siberian ibex and domestic goat hybrids and performed allele-specific expression and splicing analyses.Results showed that genes exhibiting significant imbalance between the ibex and goat were highly related to the Toll-like receptor(TLR),antigen recognition,and immune activation pathways.Comparative genomic analysis of the species revealed that immune function-related genes were under strong selection pressure in the domestic goat.
基金co-supported by the National Natural Science Foundation of China(Grant No.31470311)the Ph.D.Programs Foundation of the Ministry of Education of China(Grant No.20136101130001).
文摘China is a hotspot of relict plant species that were once widespread throughout the Northern Hemisphere.Recent research has demonstrated that the occurrence of long-term stable refugia in the mountainous regions of central and south-western China allowed their persistence through the late Neogene climate fluctuations.One of these relict lineages is Dipteronia,an oligotypic tree genus with a fossil record extending to the Paleocene.Here,we investigated the genetic variability,demographic dynamics and diversification patterns of the two currently recognized Dipteronia species(Dipteronia sinensis and D.dyeriana).Molecular data were obtained from 45 populations of Dipteronia by genotyping three cpDNA regions,two single copy nuclear genes and 15 simple sequence repeat loci.The genetic study was combined with niche comparison analyses on the environmental space,ecological niche modeling,and landscape connectivity analysis.We found that the two Dipteronia species have highly diverged both in genetic and ecological terms.Despite the incipient speciation processes that can be observed in D.sinensis,the occurrence of long-term stable refugia and,particularly,a dispersal corridor along Daba Shan-west Qinling,likely ensured its genetic and ecological integrity to date.Our study will not only help us to understand how populations of Dipteronia species responded to the tectonic and climatic changes of the Cenozoic,but also provide insight into how Arcto-Tertiary relict plants in East Asia survived,evolved,and diversified.
基金Supported by the National Natural Science Foundation of China(No.31970217)the Scientific and Technological Innovation Programs of Higher Education Institution in Shanxi(No.2019L0070)。
文摘We describe a new species of Discostella from Guiyang based on light and scanning electron microscopic observations,as well as molecular analysis.This species is morphologically distinguished from other species by a relatively smooth and slightly recessed central area with marginal striae of unequal lengths forked near the margin.Marginal fultoportulae and a single rimoportula are situated between every two costae or on the costae.Both morphological characters and the concatenated data of rbc L and SSU rDNA indicate that Discostella guiyangensis is a unique and new species.According to divergence time estimation analysis,Discostella guiyangensis is the oldest species among the Discostella species sequenced currently.In addition,we complement molecular data of Discostella asterocostata and Discostella stelligera.
基金Under the auspices of National Natural Science Foundation of China(No.42371214,42101184)Chenguang Program of Shanghai Education Development Foundation and Shanghai Municipal Education Commission(No.22CGA27)Funded Projects for the Academic Leaders and Academic Backbone,Shaanxi Normal University(No.18QNGG013)。
文摘Transportation accessibility has been treated as an important means of reducing the urban-rural income disparity.However,only a few studies have examined the effects of different types of transportation accessibility on urban-rural income disparity and their spatial heterogeneity.Based on data from 285 prefecture-level(and above)Chinese cities in 2000,2005,2010,2015,and 2020,this study uses spatial econometric models to examine how highway accessibility and railway accessibility influence the urban-rural income disparity and to identify their spatial heterogeneity.The result reveals that highway accessibility and railway accessibility have‘coreperiphery’ring-like circle structures.The urban-rural income disparity exhibits strong spatial clustering effects.Both highway accessibility and railway accessibility are negatively associated with urban-rural income disparity,and the former having a greater effect size.Moreover,there is a substitution effect between highway accessibility and railway accessibility in the whole sample.Furthermore,these associations differ in geographic regions.In the central region,highway accessibility is more important in reducing the urban-rural income disparity,but its effect is weakened with the increase of railway accessibility.In the western region,railway accessibility has a larger effect on narrowing the urban-rural income disparity,and this effect is strengthened by the increase of highway accessibility.We conclude that improving transportation accessibility is conducive to reducing the urban-rural income disparity but its effect is spatial heterogenetic.Highways and railways should be developed in a coordinated manner to promote an integrated transport network system.
基金supported by National Natural Science Foundation of China(12061080,12161087 and 12261093)the Science and Technology Project of the Education Department of Jiangxi Province(GJJ211601)supported by National Natural Science Foundation of China(11871305).
文摘We study equations in divergence form with piecewise Cαcoefficients.The domains contain corners and the discontinuity surfaces are attached to the edges of the corners.We obtain piecewise C^(1,α) estimates across the discontinuity surfaces and provide an example to illustrate the issue regarding the regularity at the corners.
基金funded by a key project of the National Natural Science Foundation of China called“Research on the Energy Process of Rapid Change of Arctic”(Grant Nos.41941012 and 41976022)the National Natural Science Foundation of China(Grant Nos.42276239 and 42106221)+1 种基金the Natural Science Foundation of Shandong Province(Grant No.ZR2022MD076)Ph.D Foundation“Variation of Arctic Sea Ice Age and Its Relationship with Atmospheric Circulation Field”(Grant No.PY112101).
文摘Besides the rapid retreating trend of Arctic sea-ice extent(SIE),this study found the most outstanding low-frequency variation of SIE to be a 4-6-year periodic variation.Using a clustering analysis algorithm,the SIE in most ice-covered regions was clustered into two special regions:Region-1 around the Barents Sea and Region-2 around the Canadian Basin,which were located on either side of the Arctic Transpolar Drift.Clear 4-6-year periodic variation in these two regions was identified using a novel method called“running linear fitting algorithm”.The rate of temporal variation of the Arctic SIE was related to three driving factors:the regional air temperature,the sea-ice areal flux across the Arctic Transpolar Drift,and the divergence of sea-ice drift.The 4-6-year periodic variation was found to have always been present since 1979,but the SIE responded to different factors under heavy and light ice conditions divided by the year 2005.The joint contribution of the three factors to SIE variation exceeded 83%and 59%in the two regions,respectively,remarkably reflecting their dynamic mechanism.It is proven that the process of El Niño-Southern Oscillation(ENSO)is closely associated with the three factors,being the fundamental source of the 4-6-year periodic variations of Arctic SIE.
基金supported by the National Natural Science Foundation of China(32060307 and 31860610)Guizhou Provincial Science and Technology Planning Project[[2021]500].
文摘In anurans,advertisement calls(ACs)are an essential form of intraspecific communication.This study evaluates geographical variation in the ACs of Leptobrachella ventripunctata in the Guizhou Plateau,southwestern China,and explores correlations between call characteristics,body size,and environmental factors.ACs are simple calls of L.ventripunctata,and apparent differences were observed in the ACs among different geographical populations of L.ventripunctata.The Call duration(CD)revealed a significant positive correlation with altitude and a significant negative correlation with temperature and humidity.Moreover,the Dominant frequency(DF)exhibited a significant negative correlation with altitude and the habitat closure degree and a significant positive correlation with temperature.These variations in ACs between different geographical populations of L.ventripunctata may critically impact the adaptive evolution of species,and the calls may also be relevant for environmental selection.
基金funded by Shanghai Natural Science Foundation(No.12ZR1414700)。
文摘Magnetic field design is essential for the operation of Hall thrusters.This study focuses on utilizing a genetic algorithm to optimize the magnetic field configuration of SPT70.A 2D hybrid PIC-DSMC and channel-wall erosion model are employed to analyze the plume divergence angle and wall erosion rate,while a Farady probe measurement and laser profilometry system are set up to verify the simulation results.The results demonstrate that the genetic algorithm contributes to reducing the divergence angle of the thruster plumes and alleviating the impact of high-energy particles on the discharge channel wall,reducing the erosion by 5.5%and 2.7%,respectively.Further analysis indicates that the change from a divergent magnetic field to a convergent magnetic field,combined with the upstream shift of the ionization region,contributes to the improving the operation of the Hall thruster.
基金the National Natural Science Foundation of China(Grant No.61973033)Preliminary Research of Equipment(Grant No.9090102010305)for funding the experiments。
文摘The longitudinal dispersion of the projectile in shooting tests of two-dimensional trajectory corrections fused with fixed canards is extremely large that it sometimes exceeds the correction ability of the correction fuse actuator.The impact point easily deviates from the target,and thus the correction result cannot be readily evaluated.However,the cost of shooting tests is considerably high to conduct many tests for data collection.To address this issue,this study proposes an aiming method for shooting tests based on small sample size.The proposed method uses the Bootstrap method to expand the test data;repeatedly iterates and corrects the position of the simulated theoretical impact points through an improved compatibility test method;and dynamically adjusts the weight of the prior distribution of simulation results based on Kullback-Leibler divergence,which to some extent avoids the real data being"submerged"by the simulation data and achieves the fusion Bayesian estimation of the dispersion center.The experimental results show that when the simulation accuracy is sufficiently high,the proposed method yields a smaller mean-square deviation in estimating the dispersion center and higher shooting accuracy than those of the three comparison methods,which is more conducive to reflecting the effect of the control algorithm and facilitating test personnel to iterate their proposed structures and algorithms.;in addition,this study provides a knowledge base for further comprehensive studies in the future.
基金supported by the Fund for Foreign Scholars in University Research and Teaching Programs(B18039)Shaanxi Youth Fund(202J-JC-QN-0668).
文摘A generalized labeled multi-Bernoulli(GLMB)filter with motion mode label based on the track-before-detect(TBD)strategy for maneuvering targets in sea clutter with heavy tail,in which the transitions of the mode of target motions are modeled by using jump Markovian system(JMS),is presented in this paper.The close-form solution is derived for sequential Monte Carlo implementation of the GLMB filter based on the TBD model.In update,we derive a tractable GLMB density,which preserves the cardinality distribution and first-order moment of the labeled multi-target distribution of interest as well as minimizes the Kullback-Leibler divergence(KLD),to enable the next recursive cycle.The relevant simulation results prove that the proposed multiple-model GLMB-TBD(MM-GLMB-TBD)algorithm based on K-distributed clutter model can improve the detecting and tracking performance in both estimation error and robustness compared with state-of-the-art algorithms for sea clutter background.Additionally,the simulations show that the proposed MM-GLMB-TBD algorithm can accurately output the multitarget trajectories with considerably less computational complexity compared with the adapted dynamic programming based TBD(DP-TBD)algorithm.Meanwhile,the simulation results also indicate that the proposed MM-GLMB-TBD filter slightly outperforms the JMS particle filter based TBD(JMSMeMBer-TBD)filter in estimation error with the basically same computational cost.Finally,the impact of the mismatches on the clutter model and clutter parameter is investigated for the performance of the MM-GLMB-TBD filter.
基金supported in part by National Key R&D Program of China under Grant No.2021YFB2900200in part by National Natural Science Foundation of China under Grant Nos.U20B2039 and 62301032in part by China Postdoctoral Science Foundation under Grant No.2023TQ0028.
文摘Integrated sensing and communication(ISAC)is regarded as a pivotal technology for 6G communication.In this paper,we employ Kullback-Leibler divergence(KLD)as the unified performance metric for ISAC systems and investigate constellation and beamforming design in the presence of clutters.In particular,the constellation design problem is solved via the successive convex approximation(SCA)technique,and the optimal beamforming in terms of sensing KLD is proven to be equivalent to maximizing the signal-to-interference-plus-noise ratio(SINR)of echo signals.Numerical results demonstrate the tradeoff between sensing and communication performance under different parameter setups.Additionally,the beampattern generated by the proposed algorithm achieves significant clutter suppression and higher SINR of echo signals compared with the conventional scheme.
基金The research was supported by a PhD scholarship of the Hungarian Ministry of Education(A.F.)a scholarship(no.2008/A/19 E)of the Hungarian Academy of Sciences(A.F.).
文摘Aims Possible shifts in the phenotypic performance along invasive plants’spreading route are rarely examined due to the discontinuous and incomplete records of exotic species.As the invasion history of common ragweed(Ambrosia artemisiifolia L.)is well documented in Hungary,its residence time is known for each location.By sampling a sequence of older to more recently established populations,we aimed to determine(i)whether there are phenotypic divergences along the historical spreading route of A.artemisiifolia;(ii)which traits are under selection during the invasion process and(iii)the extent of maternal effects on the individual’s performance.Methods We used a hierarchical sampling design to collect seeds from 64 individuals belonging to eight sites in four residence time categories(seven populations along the historical spreading route of ragweed in Hungary and one recently invaded site in Romania).We selected four large and four small individual plants in each population to control for maternal effects.The offspring were reared in a common garden located in Romania.Five vegetative phenotypic traits were measured at the end of the experiments and used in the subsequent analysis(plant height,basal diameter,number of secondary axes,length of the longest secondary axis and biomass).To summarize the variation of these highly correlated traits,principal component analysis was performed first and then the important components were used in linear mixed effect models.Important Findings The residence time categories were significantly distinguished by the first component,which compresses the variation of all five measured traits.The measures gradually decrease from populations with the longest residence time(introduced more than 65 years ago)towards the most recently established populations(established less than 30 years ago).These differences might reflect the invasion history of the populations:the longer the residence time the higher the chance to develop relevant traits beneficial in invasion process.The size of the mother plant significantly influenced not only the seed mass(inversely)but also the adult performance of its offspring(directly).
基金This work was supported by grants from the Strategic Prior-ity Research Program,Chinese Academy of Sciences(Grant No.XDA23080000)Second Tibetan Plateau Scientific Expedition and Research(STEP)program(2019QZKK0502).
文摘The genus Rhododendron(Ericaceae),with more than 1000 species highly diverse in f lower color,is providing distinct ornamental values and a model system for f lower color studies.Here,we investigated the divergence between two parental species with different f lower color widely used for azalea breeding.Gapless genome assembly was generated for the yellow-f lowered azalea,Rhododendron molle.Comparative genomics found recent proliferation of long terminal repeat retrotransposons(LTR-RTs),especially Gypsy,has resulted in a 125 Mb(19%)genome size increase in species-specific regions,and a significant amount of dispersed gene duplicates(13402)and pseudogenes(17437).Metabolomic assessment revealed that yellow f lower coloration is attributed to the dynamic changes of carotenoids/f lavonols biosynthesis and chlorophyll degradation.Time-ordered gene co-expression networks(TO-GCNs)and the comparison confirmed the metabolome and uncovered the specific gene regulatory changes underpinning the distinct f lower pigmentation.B3 and ERF TFs were found dominating the gene regulation of carotenoids/f lavonols characterized pigmentation in R.molle,while WRKY,ERF,WD40,C2H2,and NAC TFs collectively regulated the anthocyanins characterized pigmentation in the red-f lowered R simsii.This study employed a multi-omics strategy in disentangling the complex divergence between two important azaleas and provided references for further functional genetics and molecular breeding.
基金supported by the Russian Science Foundation within the Project No.21-66-00007support of the Russian Ministry of Science and Higher Education。
文摘Background The genomes of worldwide poultry breeds divergently selected for performance and other phenotypic traits may also be affected by,and formed due to,past and current admixture events.Adaptation to diverse environments,including acclimation to harsh climatic conditions,has also left selection footprints in breed genomes.Results Using the Chicken 50K_CobbCons SNP chip,we genotyped four divergently selected breeds:two aboriginal,cold tolerant Ushanka and Orloff Mille Fleur,one egg-type Russian White subjected to artificial selection for cold tolerance,and one meat-type White Cornish.Signals of selective sweeps were determined in the studied breeds using three methods:(1)assessment of runs of homozygosity islands,(2)F_(ST) based population differential analysis,and(3)haplotype differentiation analysis.Genomic regions of true selection signatures were identified by two or more methods or in two or more breeds.In these regions,we detected 540 prioritized candidate genes supplemented them with those that occurred in one breed using one statistic and were suggested in other studies.Amongst them,SOX5,ME3,ZNF536,WWP1,RIPK2,OSGIN2,DECR1,TPO,PPARGC1A,BDNF,MSTN,and beta-keratin genes can be especially mentioned as candidates for cold adaptation.Epigenetic factors may be involved in regulating some of these important genes(e.g.,TPO and BDNF).Conclusion Based on a genome-wide scan,our findings can help dissect the genetic architecture underlying various phenotypic traits in chicken breeds.These include genes representing the sine qua non for adaptation to harsh environments.Cold tolerance in acclimated chicken breeds may be developed following one of few specific gene expression mechanisms or more than one overlapping response known in cold-exposed individuals,and this warrants further investigation.
基金This study was supported by the Key Laboratory of National Forestry and Grassland Administration for Orchid Conservation and Utilization(grant number OC202103)the Harbin Normal University Postgraduate Innovation Project(grant number HSDBSCX2021-01)+1 种基金the National Natural Science Foundation of China General Projects(grant number 32170216)the Hangzhou Science and Technology Development Project(grant number 20201203B113).
文摘Cryptic species are commonly misidentified because of high morphological similarities to other species.One group of plants that may harbor large numbers of cryptic species is the quillworts(Isoetes spp.),an ancient aquatic plant lineage.Although over 350 species of Isoetes have been reported globally,only ten species have been recorded in China.The aim of this study is to better understand Isoetes species diversity in China.For this purpose,we systematically explored the phylogeny and evolution of Isoetes using complete chloroplast genome(plastome)data,spore morphology,chromosome number,genetic structure,and haplotypes of almost all Chinese Isoetes populations.We identified three ploidy levels of Isoetes in Chinaddiploid(2n=22),tetraploid(2n=44),and hexaploid(2n=66).We also found four megaspore and microspore ornamentation types in diploids,six in tetraploids,and three in hexaploids.Phylogenetic analyses confirmed that I.hypsophila as the ancestral group of the genus and revealed that Isoetes diploids,tetraploids,and hexaploids do not form monophyletic clades.Most individual species possess a single genetic structure;however,several samples have conflicting positions on the phylogenetic tree based on SNPs and the tree based on plastome data.All 36 samples shared 22 haplotypes.Divergence time analysis showed that I.hypsophila diverged in the early Eocene(~48.05 Ma),and most other Isoetes species diverged 3-20 Ma.Additionally,different species of Isoetes were found to inhabit different water systems and environments along the Yangtze River.These findings provide new insights into the relationships among Isoetes species in China,where highly similar morphologic populations may harbor many cryptic species.
基金supported by the National Natural Science Foundation of China (Grant No.31461123001 to C.X.F.)the US National Science Foundation Dimensions of Biodiversity US-China Program (DEB-1442280 to P.S.S.and D.E.S.)+1 种基金the HZAU Talent Start-up Fund (Grant no.11042210014 to M.S.)the China Scholarship Council (Grant no.201806320056 to H.Y.L.)。
文摘The underlying causes of biodiversity disparities among geographic regions have long been a fundamental theme in ecology and evolution.However,the patterns of phylogenetic diversity(PD) and phylogenetic beta diversity(PBD) of congeners that are disjunctly distributed between eastern Asia-eastern North America(EA-ENA disjuncts) and their associated factors remain unknown.Here we investigated the standardized effect size of PD(SES-PD),PBD,and potentially associated factors in 11natural mixed forest sites(five in EA and six in ENA) where abundant EA-ENA disjuncts occur.We found that the disjuncts in ENA possessed higher SES-PD than those in EA at the continental scale(1.96vs-1.12),even though the number of disjunct species in ENA is much lower than in EA(128 vs 263).SESPD of the EA-ENA disjuncts tended to decrease with increasing latitude in 11 sites.The latitudinal diversity gradient of SES-PD was stronger in EA sites than in ENA sites.Based on the unweighted unique fraction metric(UniFrac) distance and the phylogenetic community dissimilarity,PBD showed that the two northern sites in EA were more similar to the six-site ENA group than to the remaining southern EA sites.Based on the standardized effect size of mean pairwise distances(SES-MPD),nine of eleven studied sites showed a neutral community structure(-1.96 ≤SES-MPD ≤1.96).Both Pearson’s r and structural equation modeling suggested that SES-PD of the EA-ENA disjuncts was mostly associated with mean divergence time.Moreover,SES-PD of the EA-ENA disjuncts was positively correlated with temperaturerelated climatic factors,although negatively correlated with mean diversification rate and community structure.By applying approaches from phylogenetics and community ecology,our work sheds light on historical patterns of the EA-ENA disjunction and paves the way for further research.
基金partially sponsored by the Natural Science Foundation of Shanghai(No.23ZR1429300)the Innovation Fund of CNNC(Lingchuang Fund)。
文摘The estimation of model parameters is an important subject in engineering.In this area of work,the prevailing approach is to estimate or calculate these as deterministic parameters.In this study,we consider the model parameters from the perspective of random variables and describe the general form of the parameter distribution inference problem.Under this framework,we propose an ensemble Bayesian method by introducing Bayesian inference and the Markov chain Monte Carlo(MCMC)method.Experiments on a finite cylindrical reactor and a 2D IAEA benchmark problem show that the proposed method converges quickly and can estimate parameters effectively,even for several correlated parameters simultaneously.Our experiments include cases of engineering software calls,demonstrating that the method can be applied to engineering,such as nuclear reactor engineering.
基金the National Key R&D Program of China(2021YFC2600400 and 2022YFC2601100)National Natural Science Foundation of China(31772236 and 31972344)Science and Technology Innovation Program of Chinese Academy of Agricultural Sciences(caascx-2022–2025IAS)。
文摘Leaf-mining flies(Diptera:Agromyzidae)are a diverse family of small-bodied insects that feed on living plant tissues as larvae.Various species in this family are considered globally invasive and have caused great agricultural economic losses.In China,economically important vegetable crops have been seriously damaged by these pest insects,especially by species of the genus Liriomyza.However,these species are difficult to differentiate because of their morphological similarities,and the Chinese fauna remains poorly known.To explore the relevant pest species in China and their phylogeny,agromyzid leafminers were collected from 2016 to 2019,and identified based on morphological characteristics and DNA barcodes.In total,27 species from five genera of Agromyzidae were sampled and identified,including 16species of Liriomyza.Both mitochondrial and nuclear genes were used to reconstruct their phylogenetic relationships and estimate the divergence time.Highly congruent and well-supported phylogenetic trees were obtained using the Bayesian inference and maximum-likelihood methods.This analysis revealed two main clades in Liriomyza,and clade 2was inferred to have diverged from clade 1 approximately 27.40 million years ago(95%highest posterior density:23.03–31.52 million years ago)in the Oligocene.Differences were observed in the distribution patterns and host associations between the Liriomyza clades.Clade 2 species are distributed in cool,high-latitude environments,suggesting that they may have evolved into a cool-adapted lineage.
基金supported by the National Key Research and Development Program of China(2018YFD1000706,2018YFD1000700).
文摘Sorghum[Sorghum bicolor(L.)Moench],a multipurpose C4 crop,is also a model species of the Poaceae family for plant research.During the process of domestication,the modification of seed dispersal mode is considered a key event,as the loss of seed shattering caused a significant increase in yield.In order to understand the seed shattering process in sorghum,we further studied eight previously identified divergent sorghum germplasm with different shattering degrees.We described their phenotypes in great detail,analyzed the histology of abscission zone,and conducted a gene co-expression analysis.We observed that the abscission layer of the most strong-shattering varieties began to differentiate before the 5-10 cm panicles development stage and was completely formed at flag leaf unfolding.The protective cells on the pedicels were also fully lignified by flowering.Through the weighted gene correlation network analysis(WGCNA),we mined for candidate genes involved in the abscission process at the heading stage.We found that these genes were mainly associated with such biological processes as hormone signal transmission(SORBI_3003G361300,SORBI_3006G216500,SORBI_3009G027800,SORBI_3007G077200),cell wall modification and degradation(SORBI_3002G205500,SORBI_3004G013800,SORBI_3010G022400,SORBI_3003G251800,SORBI_3003G254700,SORBI_3003G410800,SORBI_3009G162700,SORBI_3001G406700,SORBI_3004G042700,SORBI_3004G244600,SORBI_3001G099100),and lignin synthesis(SORBI_3004G220700,SORBI_3004G062500,SORBI_3010G214900,SORBI_3009G181800).Our study has provided candidate genes required for shedding for further study.We believe that function characterization of these genes may provide insight into our understanding of seed shattering process.