Frame and frequency synchronization are essential for orthogonal frequency division multiplexing (OFDM) systems. The frame offset owing to incorrect start point position of the fast Fourier transform (FFT) window,...Frame and frequency synchronization are essential for orthogonal frequency division multiplexing (OFDM) systems. The frame offset owing to incorrect start point position of the fast Fourier transform (FFT) window, and the carrier frequency offset (CFO) due to Doppler frequency shift or the frequency mismatch between the transmitter and receiver oscil ators, can bring severe inter-symbol interference (ISI) and inter-carrier interference (ICI) for the OFDM system. Relying on the relatively good correlation charac-teristic of the pseudo-noise (PN) sequence, a joint frame offset and normalized CFO estimation algorithm based on PN preamble in time domain is developed to realize the frame and frequency synchronization in the OFDM system. By comparison, the perfor-mances of the traditional algorithm and the improved algorithm are simulated under different conditions. The results indicate that the PN preamble based algorithm both in frame offset estimation and CFO estimation is more accurate, resource-saving and robust even under poor channel condition, such as low signal-to-noise ratio (SNR) and large normalized CFO.展开更多
A nonlinear damage model based on the combination of deformation and hysteretic energy and its validation with experiments are presented.Also,a combination parameter is defined to consider the mutual effect of deforma...A nonlinear damage model based on the combination of deformation and hysteretic energy and its validation with experiments are presented.Also,a combination parameter is defined to consider the mutual effect of deformation and hysteretic energy for different types of components in different loading stages.Four reinforced concrete (RC) columns are simulated and analyzed using the nonlinear damage model.The results indicate that the damage evolution evaluated by the model agrees well with the experimental phenomenon.Furthermore,the seismic damage evolution of a six-story RC frame was analyzed,revealing four typical failure modes according to the interstory drift distribution of the structure;the damage values calculated using the nonlinear damage model agree well with the four typical failure modes.展开更多
Based on four intra-technique combined solutions in SINEX format, we tried at first to assess the accuracy of each single-technique Earth Orientation Parameters(EOPs) series over a past time interval of at least 15 ye...Based on four intra-technique combined solutions in SINEX format, we tried at first to assess the accuracy of each single-technique Earth Orientation Parameters(EOPs) series over a past time interval of at least 15 years by comparison with IERS EOP 08C04 combined solution as the reference in this paper. The EOPs studied here mainly consist of four elements, i.e. polar motion(XPO, YPO), Universal Time(UT1-UTC) and length-of-day(LOD). We combined these intra-technique EOP series, each of them associated with a given space geodetic technique by taking advantage of the relationship of the first three EOP components and three rotational parameters which carry the orientation of technique-related reference frame with respect to the estimated weekly inter-technique combined reference frame. Results indicated that the discrepancy between the pole coordinates(XPO, YPO) series, extracted from the intra-SLR combined loose-constraint solutions and the IERS EOP 08C04, seemed to be clearly characterized by systematic errors. Although both the XPO and YPO series determined by intra-VLBI combination had no significant characteristic of system error, they had relatively large difference values at some point with respect to the IERS EOP 08C04, which may be limited by the quantity of observation stations. Since the number of GPS stations is on the increase aimed at better global coverage, the accuracy of pole coordinates provided by IGS was superior to that derived from other space-geodetic techniques. As for DORIS XPO and YPO series from intra-DORIS combined minimal-constraint solutions, the discrepancy range of the former with respect to IERS EOP 08C04 was a little smaller than that of the latter. The objective of this study is twofold: on the one hand to analysis individual EOP series derived from the various space-geodetic techniques, on the other hand to present the new inter-technique combined EOP solution consistent with weekly inter-technique combined reference frame.展开更多
AIM: To isolate a novel isoform of human HPO (HPO-205) from human fetal liver Marathon-ready cDNA and characterize its primary biological function. METHODS: 5'-RACE (rapid amplification of cDNA 5' ends) was us...AIM: To isolate a novel isoform of human HPO (HPO-205) from human fetal liver Marathon-ready cDNA and characterize its primary biological function. METHODS: 5'-RACE (rapid amplification of cDNA 5' ends) was used to isolate a novel isoform of hHPO in this paper. The constructed pcDNA(HPO-205), pcDNA(HPO) and pcDNA eukaryotic expression vectors were respectively transfected by lipofectamine method and the stimulation of DNA synthesis was observed by (3)H-TdR incorporation assay. Proteins extracted from different cells were analyzed by Western blot. RESULTS: A novel isoform of hHPO (HPO-205) encoding a 205 amino acid ORF corresponding to a translated production of 23 kDa was isolated and distinguished from the previous HPO that lacked the N-terminal 80 amino acids. The dose-dependent stimulation of DNA synthesis of HepG2 hepatoma cells by HPO-205 demonstrated its similar biological activity with HPO in vitro. The level of MAPK (Mitogen-activated protein kinase) phosphorylation by Western blot analysis revealed that HPO-205 might have the stronger activity of stimulating hepatic cell proliferation than that of HPO. CONCLUSION: A novel isoform of hHPO (HPO-205) was isolated from hepatic-derived cells. The comparison of HPO-205 and HPO will lead to a new insight into the structure and function of hHPO, and provide the new way of thinking to deeply elucidate the biological roles of HPO/ALR.展开更多
AIM:There are conflicting data about p53 function on cellular sensitivity to the cytotoxic action of 5-fluorouracil (5-FU). Therefore the objective of this study was to determine the combined effects of adenovirus-med...AIM:There are conflicting data about p53 function on cellular sensitivity to the cytotoxic action of 5-fluorouracil (5-FU). Therefore the objective of this study was to determine the combined effects of adenovirus-mediated wild-type (wt) p53 gene transfer and 5-FU chemotherapy on pancreatic cancer cells with different p53 gene status. METHODS:Human pancreatic cancer cell lines Capan-1^(p53mut), Capan-2^(p53wt),FAMPAC^(p53mut),PANC1^(p53mut),and rat pancreatic cancer cell lines AS^(p53wt) and DSL6A^(p53null) were used for in vitro studies.Following infection with different ratios of Ad- p53-particles (MOI) in combination with 5-FU,proliferation of tumor cells and apoptosis were quantified by cell proliferation assay (WST-1) and FACS (PI-staining).In addition,DSL6A syngeneic pancreatic tumor cells were inoculated subcutaneously in to Lewis rats for in vivo studies. Tumor size,apoptosis (TUNEL) and survival were determined. RESULTS:Ad-p53 gene transfer combined with 5-FU significantly inhibited tumor cell proliferation and substantially enhanced apoptosis in all four cell lines with an alteration in the p53 gene compared to those two cell lines containing wt-p53.In vivo experiments showed the most effective tumor regression in animals treated with Ad-p53 plus 5-FU.Both in vitro and in vivo analyses revealed that a sublethal dose of Ad-p53 augmented the apoptotic response induced by 5-FU. CONCLUSION:Our results suggest that Ad-p53 may synergistically enhance 5-FU-chemosensitivity most strikingly in pancreatic cancer cells lacking p53 function.These findings illustrate that the anticancer efficacy of this combination treatment is dependent on the p53 gene status of the target tumor cells.展开更多
i) Instead of x ̄n+ y ̄n = z ̄n ,we use as the general equation of Fermat's Last Theorem (FLT),where a and b are two arbitrary natural numbers .By means of binomial expansion ,(0.1) an be written as Because a ̄...i) Instead of x ̄n+ y ̄n = z ̄n ,we use as the general equation of Fermat's Last Theorem (FLT),where a and b are two arbitrary natural numbers .By means of binomial expansion ,(0.1) an be written as Because a ̄r-(-b) ̄r always contains a +b as its factor ,(0.2) can be written as where φ_r =[a ̄r-(-b) ̄r]/ (a+b ) are integers for r=1 . 2, 3. ...n (ii) Lets be a factor of a+b and let (a +b) = se. We can use x= sy to transform (0.3 ) to the following (0.4)(iii ) Dividing (0.4) by s ̄2 we have On the left side of (0.5) there is a polynomial of y with integer coefficient and on the right side there is a constant cφ/s .If cφ/s is not an integer ,then we cannot find an integer y to satisfy (0.5), and then FLT is true for this case. If cφ_n/s is an integer ,we may change a and c such the cφ_n/s≠an integer .展开更多
To improve frame synchronization precision, a scheme named training symbol correlation (TSC) is presented for orthogonal frequency division multiplexing ( OFDM ) system. Based on the solution from Schmidl and Cox,...To improve frame synchronization precision, a scheme named training symbol correlation (TSC) is presented for orthogonal frequency division multiplexing ( OFDM ) system. Based on the solution from Schmidl and Cox, a timing metric related to TSC scheme is put forward and examined. The specific method to select a threshold value provides more precise detection results, which can be shown by performance comparison between the two schemes through Monte Carlo simulation. Taking IEEE 802.1 la WLAN standard as an example, the proposed approach is superior to the most popular Schmidl scheme in terms of BER.展开更多
An ultrafast framing camera with a pulse-dilation device,a microchannel plate(MCP)imager,and an electronic imaging system were reported.The camera achieved a temporal resolution of 10 ps by using a pulse-dilation devi...An ultrafast framing camera with a pulse-dilation device,a microchannel plate(MCP)imager,and an electronic imaging system were reported.The camera achieved a temporal resolution of 10 ps by using a pulse-dilation device and gated MCP imager,and a spatial resolution of 100μm by using an electronic imaging system comprising combined magnetic lenses.The spatial resolution characteristics of the camera were studied both theoretically and experimentally.The results showed that the camera with combined magnetic lenses reduced the field curvature and acquired a larger working area.A working area with a diameter of 53 mm was created by applying four magnetic lenses to the camera.Furthermore,the camera was used to detect the X-rays produced by the laser-targeting device.The diagnostic results indicated that the width of the X-ray pulse was approximately 18 ps.展开更多
To achieve virtual full-duplex(VFD)communication using half-duplex radios,the rapid on-off-division(RODD)technique has been proposed in recent years.The time-hopping(TH)sequence is critical to controlling self-interfe...To achieve virtual full-duplex(VFD)communication using half-duplex radios,the rapid on-off-division(RODD)technique has been proposed in recent years.The time-hopping(TH)sequence is critical to controlling self-interference introduced in the paradigm.By constructing the collision model with a symbol level time scale,the periodic collision correlation function properties are introduced as the performance metric for the TH sequence in the RODD system.To achieve the best VFD performance,an optimization-based method for TH sequence design is proposed.In addition,the conventional TH frame structure design for RODD system is improved.Numerical simulations are presented to demonstrate that the proposed approach can significantly increase system performance.Results indicate that the TH sequence design is very effective for the RODD system.展开更多
在正交频分复用(orthogonal frequency division multiplexing,OFDM)系统接收端,错误估计快速傅里叶变换窗口起始点会导致OFDM帧偏差,从而造成严重的符号间干扰(inter symbol interference,ISI)以及子载波间干扰(inter carrier interfer...在正交频分复用(orthogonal frequency division multiplexing,OFDM)系统接收端,错误估计快速傅里叶变换窗口起始点会导致OFDM帧偏差,从而造成严重的符号间干扰(inter symbol interference,ISI)以及子载波间干扰(inter carrier interference,ICI)。首先建立一个带有帧同步的OFDM系统模型,然后通过理论推导得到帧偏差对OFDM系统的影响。利用伪随机序列自身较好的相关特性,设计了一种基于伪随机序列前导码的帧同步算法,并分析了3种基于循环前缀(cyclic prefix,CP)的传统帧同步算法。通过仿真实验,结果表明所提出的基于伪随机序列前导码的帧同步算法与传统算法相比,即使在较差的信道条件下,如在较低的信噪比或存在较大的载波频率偏移(carrier frequency offset,CFO)时,其帧偏差估计结果更为准确、稳定以及节省资源。展开更多
基于通信的列车控制(Communication-Based Train Control,CBTC)系统是一种采用先进的通信、计算机技术,连续控制、监测列车运行的列车控制系统.为了提高CBTC系统的传输效率,将正交频分复用技术(Orthogonal Frequency Division Multiplex...基于通信的列车控制(Communication-Based Train Control,CBTC)系统是一种采用先进的通信、计算机技术,连续控制、监测列车运行的列车控制系统.为了提高CBTC系统的传输效率,将正交频分复用技术(Orthogonal Frequency Division Multiplexing,OFDM)运用于CBTC系统,从OFDM系统的工作原理出发,研究了列车速度对于OFDM系统子载波非正交性的影响,并以无线局域网(Wireless Local Area Networks,WLAN)技术中的OFDM参数为例,对列车速度引起的信道相干时间变化进行了深入分析与计算.理论计算与仿真结果表明:列车速度对子载波间正交性的影响可以忽略不计;在同样报文长度、不同列车速度下,最低的传输速率并不一定能得到最低的误帧率(Frame Error Rate,FER);通信系统应该根据信道时变情况以及报文长度分别选择不同的传输速率以得到最低的FER.展开更多
基金supported by the National Natural Science Foundation of China(6130110561102069)+2 种基金the China Postdoctoral Science Foundation Funded Project(2013M531351)the Nanjing University of Aeronautics and Astronautics Founding(NN2012022)the Open Fund of Graduate Innovated Base(Laboratory)for the Nanjing University of Aeronautics and Astronautics(KFJJ120219)
文摘Frame and frequency synchronization are essential for orthogonal frequency division multiplexing (OFDM) systems. The frame offset owing to incorrect start point position of the fast Fourier transform (FFT) window, and the carrier frequency offset (CFO) due to Doppler frequency shift or the frequency mismatch between the transmitter and receiver oscil ators, can bring severe inter-symbol interference (ISI) and inter-carrier interference (ICI) for the OFDM system. Relying on the relatively good correlation charac-teristic of the pseudo-noise (PN) sequence, a joint frame offset and normalized CFO estimation algorithm based on PN preamble in time domain is developed to realize the frame and frequency synchronization in the OFDM system. By comparison, the perfor-mances of the traditional algorithm and the improved algorithm are simulated under different conditions. The results indicate that the PN preamble based algorithm both in frame offset estimation and CFO estimation is more accurate, resource-saving and robust even under poor channel condition, such as low signal-to-noise ratio (SNR) and large normalized CFO.
基金the National Natural Science Foundation of China(Grant 51578058)the Beijing Natural Science Foundation(Grant 8172038).
文摘A nonlinear damage model based on the combination of deformation and hysteretic energy and its validation with experiments are presented.Also,a combination parameter is defined to consider the mutual effect of deformation and hysteretic energy for different types of components in different loading stages.Four reinforced concrete (RC) columns are simulated and analyzed using the nonlinear damage model.The results indicate that the damage evolution evaluated by the model agrees well with the experimental phenomenon.Furthermore,the seismic damage evolution of a six-story RC frame was analyzed,revealing four typical failure modes according to the interstory drift distribution of the structure;the damage values calculated using the nonlinear damage model agree well with the four typical failure modes.
基金supported by the National Natural Science Foundation of China (grants 11773058)
文摘Based on four intra-technique combined solutions in SINEX format, we tried at first to assess the accuracy of each single-technique Earth Orientation Parameters(EOPs) series over a past time interval of at least 15 years by comparison with IERS EOP 08C04 combined solution as the reference in this paper. The EOPs studied here mainly consist of four elements, i.e. polar motion(XPO, YPO), Universal Time(UT1-UTC) and length-of-day(LOD). We combined these intra-technique EOP series, each of them associated with a given space geodetic technique by taking advantage of the relationship of the first three EOP components and three rotational parameters which carry the orientation of technique-related reference frame with respect to the estimated weekly inter-technique combined reference frame. Results indicated that the discrepancy between the pole coordinates(XPO, YPO) series, extracted from the intra-SLR combined loose-constraint solutions and the IERS EOP 08C04, seemed to be clearly characterized by systematic errors. Although both the XPO and YPO series determined by intra-VLBI combination had no significant characteristic of system error, they had relatively large difference values at some point with respect to the IERS EOP 08C04, which may be limited by the quantity of observation stations. Since the number of GPS stations is on the increase aimed at better global coverage, the accuracy of pole coordinates provided by IGS was superior to that derived from other space-geodetic techniques. As for DORIS XPO and YPO series from intra-DORIS combined minimal-constraint solutions, the discrepancy range of the former with respect to IERS EOP 08C04 was a little smaller than that of the latter. The objective of this study is twofold: on the one hand to analysis individual EOP series derived from the various space-geodetic techniques, on the other hand to present the new inter-technique combined EOP solution consistent with weekly inter-technique combined reference frame.
基金the National Natural Science Foundation of China,No.39830440
文摘AIM: To isolate a novel isoform of human HPO (HPO-205) from human fetal liver Marathon-ready cDNA and characterize its primary biological function. METHODS: 5'-RACE (rapid amplification of cDNA 5' ends) was used to isolate a novel isoform of hHPO in this paper. The constructed pcDNA(HPO-205), pcDNA(HPO) and pcDNA eukaryotic expression vectors were respectively transfected by lipofectamine method and the stimulation of DNA synthesis was observed by (3)H-TdR incorporation assay. Proteins extracted from different cells were analyzed by Western blot. RESULTS: A novel isoform of hHPO (HPO-205) encoding a 205 amino acid ORF corresponding to a translated production of 23 kDa was isolated and distinguished from the previous HPO that lacked the N-terminal 80 amino acids. The dose-dependent stimulation of DNA synthesis of HepG2 hepatoma cells by HPO-205 demonstrated its similar biological activity with HPO in vitro. The level of MAPK (Mitogen-activated protein kinase) phosphorylation by Western blot analysis revealed that HPO-205 might have the stronger activity of stimulating hepatic cell proliferation than that of HPO. CONCLUSION: A novel isoform of hHPO (HPO-205) was isolated from hepatic-derived cells. The comparison of HPO-205 and HPO will lead to a new insight into the structure and function of hHPO, and provide the new way of thinking to deeply elucidate the biological roles of HPO/ALR.
文摘AIM:There are conflicting data about p53 function on cellular sensitivity to the cytotoxic action of 5-fluorouracil (5-FU). Therefore the objective of this study was to determine the combined effects of adenovirus-mediated wild-type (wt) p53 gene transfer and 5-FU chemotherapy on pancreatic cancer cells with different p53 gene status. METHODS:Human pancreatic cancer cell lines Capan-1^(p53mut), Capan-2^(p53wt),FAMPAC^(p53mut),PANC1^(p53mut),and rat pancreatic cancer cell lines AS^(p53wt) and DSL6A^(p53null) were used for in vitro studies.Following infection with different ratios of Ad- p53-particles (MOI) in combination with 5-FU,proliferation of tumor cells and apoptosis were quantified by cell proliferation assay (WST-1) and FACS (PI-staining).In addition,DSL6A syngeneic pancreatic tumor cells were inoculated subcutaneously in to Lewis rats for in vivo studies. Tumor size,apoptosis (TUNEL) and survival were determined. RESULTS:Ad-p53 gene transfer combined with 5-FU significantly inhibited tumor cell proliferation and substantially enhanced apoptosis in all four cell lines with an alteration in the p53 gene compared to those two cell lines containing wt-p53.In vivo experiments showed the most effective tumor regression in animals treated with Ad-p53 plus 5-FU.Both in vitro and in vivo analyses revealed that a sublethal dose of Ad-p53 augmented the apoptotic response induced by 5-FU. CONCLUSION:Our results suggest that Ad-p53 may synergistically enhance 5-FU-chemosensitivity most strikingly in pancreatic cancer cells lacking p53 function.These findings illustrate that the anticancer efficacy of this combination treatment is dependent on the p53 gene status of the target tumor cells.
文摘i) Instead of x ̄n+ y ̄n = z ̄n ,we use as the general equation of Fermat's Last Theorem (FLT),where a and b are two arbitrary natural numbers .By means of binomial expansion ,(0.1) an be written as Because a ̄r-(-b) ̄r always contains a +b as its factor ,(0.2) can be written as where φ_r =[a ̄r-(-b) ̄r]/ (a+b ) are integers for r=1 . 2, 3. ...n (ii) Lets be a factor of a+b and let (a +b) = se. We can use x= sy to transform (0.3 ) to the following (0.4)(iii ) Dividing (0.4) by s ̄2 we have On the left side of (0.5) there is a polynomial of y with integer coefficient and on the right side there is a constant cφ/s .If cφ/s is not an integer ,then we cannot find an integer y to satisfy (0.5), and then FLT is true for this case. If cφ_n/s is an integer ,we may change a and c such the cφ_n/s≠an integer .
文摘To improve frame synchronization precision, a scheme named training symbol correlation (TSC) is presented for orthogonal frequency division multiplexing ( OFDM ) system. Based on the solution from Schmidl and Cox, a timing metric related to TSC scheme is put forward and examined. The specific method to select a threshold value provides more precise detection results, which can be shown by performance comparison between the two schemes through Monte Carlo simulation. Taking IEEE 802.1 la WLAN standard as an example, the proposed approach is superior to the most popular Schmidl scheme in terms of BER.
基金National Natural Science Foundation of China(NSFC)(No.11775147)Guangdong Basic and Applied Basic Research Foundation(Nos.2019A1515110130 and 2024A1515011832)+1 种基金Shenzhen Key Laboratory of Photonics and Biophotonics(ZDSYS20210623092006020)Shenzhen Science and Technology Program(Nos.JCYJ20210324095007020,JCYJ20200109105201936 and JCYJ20230808105019039).
文摘An ultrafast framing camera with a pulse-dilation device,a microchannel plate(MCP)imager,and an electronic imaging system were reported.The camera achieved a temporal resolution of 10 ps by using a pulse-dilation device and gated MCP imager,and a spatial resolution of 100μm by using an electronic imaging system comprising combined magnetic lenses.The spatial resolution characteristics of the camera were studied both theoretically and experimentally.The results showed that the camera with combined magnetic lenses reduced the field curvature and acquired a larger working area.A working area with a diameter of 53 mm was created by applying four magnetic lenses to the camera.Furthermore,the camera was used to detect the X-rays produced by the laser-targeting device.The diagnostic results indicated that the width of the X-ray pulse was approximately 18 ps.
基金Supported by the National Key Basic Research Program of China(973 Program)(613314)
文摘To achieve virtual full-duplex(VFD)communication using half-duplex radios,the rapid on-off-division(RODD)technique has been proposed in recent years.The time-hopping(TH)sequence is critical to controlling self-interference introduced in the paradigm.By constructing the collision model with a symbol level time scale,the periodic collision correlation function properties are introduced as the performance metric for the TH sequence in the RODD system.To achieve the best VFD performance,an optimization-based method for TH sequence design is proposed.In addition,the conventional TH frame structure design for RODD system is improved.Numerical simulations are presented to demonstrate that the proposed approach can significantly increase system performance.Results indicate that the TH sequence design is very effective for the RODD system.
文摘在正交频分复用(orthogonal frequency division multiplexing,OFDM)系统接收端,错误估计快速傅里叶变换窗口起始点会导致OFDM帧偏差,从而造成严重的符号间干扰(inter symbol interference,ISI)以及子载波间干扰(inter carrier interference,ICI)。首先建立一个带有帧同步的OFDM系统模型,然后通过理论推导得到帧偏差对OFDM系统的影响。利用伪随机序列自身较好的相关特性,设计了一种基于伪随机序列前导码的帧同步算法,并分析了3种基于循环前缀(cyclic prefix,CP)的传统帧同步算法。通过仿真实验,结果表明所提出的基于伪随机序列前导码的帧同步算法与传统算法相比,即使在较差的信道条件下,如在较低的信噪比或存在较大的载波频率偏移(carrier frequency offset,CFO)时,其帧偏差估计结果更为准确、稳定以及节省资源。
文摘基于通信的列车控制(Communication-Based Train Control,CBTC)系统是一种采用先进的通信、计算机技术,连续控制、监测列车运行的列车控制系统.为了提高CBTC系统的传输效率,将正交频分复用技术(Orthogonal Frequency Division Multiplexing,OFDM)运用于CBTC系统,从OFDM系统的工作原理出发,研究了列车速度对于OFDM系统子载波非正交性的影响,并以无线局域网(Wireless Local Area Networks,WLAN)技术中的OFDM参数为例,对列车速度引起的信道相干时间变化进行了深入分析与计算.理论计算与仿真结果表明:列车速度对子载波间正交性的影响可以忽略不计;在同样报文长度、不同列车速度下,最低的传输速率并不一定能得到最低的误帧率(Frame Error Rate,FER);通信系统应该根据信道时变情况以及报文长度分别选择不同的传输速率以得到最低的FER.